Functional (non-ulcerative) dyspepsia

The purpose of the study is to conduct a survey among students, to study the leading symptoms, as well as to identify risk factors in its development.Цель исследования – изучить ведущие симптомы и факторы риска развития ФД

Проявления апоптоза в субпопуляциях циркулирующих опухолевых клеток с фенотипами, ассоциированными со стволовостью и эпителиально-мезенхимальным переходом, при карциноме молочной железы

Introduction. Ability of circulating tumor cells (CTC) initiate metastases in distant sites is associated primarily with their resistance to apoptosis which allows them to retain viability in the blood. Knowledge of phenotypical signs associated with this ability would allow to predict the risk of metastases and optimize adjuvant therapy.Aim. To examine signs of apoptosis in CTC populations with various phenotypical characteristics.Materials and methods. The study included 58 patients with invasive breast carcinoma of unspecified type, stages T1–4N0–3M0. Cell concentrates extracted from patients’ whole blood were stained with an antibody cocktail against CK7 / 8, CD45, EpCAM, CD44, CD24, CD133, ALDH, N-cadherin which allowed to identify CTC with signs of stemness and epithelial-mesenchymal transition. Annexin V and 7‑amino-actinomycin D staining was used for evaluation of apoptosis stage in CTC populations.Results. Circulating tumor cells are characterized by heterogeneity in respect to signs of stemness and epithelial-mesenchymal transition and presence of early and late signs of apoptosis and necrosis. CTC phenotypes including co-expression of epithelial marker CK7 / 8 and stemness marker CD133 (but not CD44) are characterized by absence of signs of apoptosis. Co-expression of CK7 / 8 and CD133 in CTC with stemness markers CD44+ / C D24– is associated with development of early but not late signs of apoptosis and necrosis. Circulating tumor cells without co-expression of CK7 / 8 and CD133 could have both early and late signs of apoptosis and necrosis. Circulating tumor cells phenotypes with signs of early apoptosis expressing CD133 remain in blood after non-adjuvant chemotherapy opposed to CTC without CD133 expression.Conclusion. There are CTC phenotypical signs associated with stemness and epithelial-mesenchymal transition and linked to apoptosis resistance or sensitivity.Введение. Способность циркулирующих опухолевых клеток (ЦОК) инициировать метастазирование в отдаленных сайтах, прежде всего, связана с их резистентностью к апоптозу, что позволяет сохранить жизнеспособность в кровотоке. Знание фенотипических признаков, связанных с этой способностью, позволило бы прогнозировать риск развития метастазов и оптимизировать адъювантную терапию.Цель исследования – изучение проявлений апоптоза в популяциях ЦОК с различными фенотипическими характеристиками.Материалы и методы. В исследование включены 58 пациенток с инвазивной карциномой молочной железы неспецифического типа стадии T1–4N0–3M0. Клеточные концентраты, полученные из цельной крови пациентов, окрашены коктейлем антител к C K7 / 8, CD45, EpCAM, CD44, CD24, CD133, ALDH, N-кадгерину, что позволяет идентифицировать ЦОК с признаками стволовости и эпителиально-мезенхимального перехода. Окрашивание аннексином V и 7-аминоактиномицином D использовали для оценки разных стадий апоптоза каждой из популяций ЦОК.Результаты. Циркулирующие опухолевые клетки характеризуются выраженной гетерогенностью по проявлениям признаков стволовости и эпителиально-мезенхимального перехода и ассоциации с наличием ранних и поздних признаков апоптоза и некроза. Для ЦОК с фенотипами, включающими коэкспрессию эпителиального маркера CK7 / 8 и маркера стволовости CD133 (но не C D44), более характерно отсутствие признаков апоптоза. Коэкспрессия CK7 / 8 и C D133 ЦОК с маркерами стволовости CD44+ / C D24– ассоциирована с развитием ранних, но не поздних признаков апоптоза и некроза. Циркулирующие опухолевые клетки с отсутствием коэкспрессии CK7 / 8 и C D133 могли иметь и ранние, и поздние признаки апоптоза и некроза. Фенотипы ЦОК с признаками раннего апоптоза, экспрессирующие CD133, в отличие от ЦОК без экспрессии CD133, сохраняются в крови после неоадъювантной химиотерапии.Заключение. Существуют фенотипические признаки ЦОК, имеющие отношение к стволовости и эпителиально-мезенхимальному переходу, сопряженные с устойчивостью к апоптозу или чувствительностью к нему

Proceedings of the 24th Paediatric Rheumatology European Society Congress: Part three

From Springer Nature via Jisc Publications Router.Publication status: PublishedHistory: collection 2017-09, epub 2017-09-0

The influence of 1-α-oxycholesterol with antioxidants and calcium phosphate on the development of experimental periodontitis in rats

In experiments on 26 rats the influence of complex 1-α-oxycholesterol with antioxidants and calcium phosphate. The study was conducted on the background modeling of periodontal disease through a combination of prooxidants delagila and etilendiaminova acid chelating agent (EDTA)

Information Technologies of Optimizing Designs and Manufacturing Techniques of Rubber-metal Products

It is shown that a high percentage of defective rubber-metal articles at the manufacturing process output is caused by neglecting the subsystem parameters connectivity at the design stage separately within the design and technology, as well as between these subsystems.The research is aimed at increasing the rubber-metal articles production stability and improving the rubber products quality through the development and introduction of a new integrated approach to the design and technology parameters optimization.In the general system of the integrated design of rubber-metal shock-absorbers, the subsystems of designs and manufacturing technologies are singled out, and the correlations between the parameters within these subsystems and the parameters of different subsystems are identified.Optimization problems have different objective functions, in which arguments often coincide fully or within certain boundaries. This significantly complicates calculations since the optimization problem in this case is often multiobjective and multiextremal. To solve this problem, the method that involves complex evolutionary optimization by means of a genetic algorithm is applied.For this, new attributes of the genetic algorithm are created. In particular, new star-shaped character models (chromosomes), with internal links between individual parents and flexible constraints on the variation of the latter during optimization are developed. The result is a paradoxical conclusion: there is an additional possibility to perform multi-criteria optimization of the design and manufacturing technology of rubber-metal articles deeper than with Pareto optimization because Pareto optimization involves a single value for all iterations of search of objective functions in the evolutionary optimization, and the arguments on each iteration may differ on some, connection depth-dependant value when using the proposed method

T15

Despite the abundance of theoretical evidences displaying the considerable role of the “soil” in metastasis progression, the majority of cancer research and cancer therapy pathogenetic methods focused mainly on the tumor cells. There is still no clear clinical data showing the role of “soil” in the metastasis. Identification of factors and mechanisms driving the conditions promoting tumor dissemination may lead to therapeutic strategy to detect and prevent metastases at the earliest step. It has been established that bone marrow-derived hematopoietic progenitor cells home to pre-metastatic sites before tumor cells infiltrate in these sites. These hematopoietic progenitor cells form regulatory cell cluster of stromal microenvironment (premetastatic niche) to promote secondary tumor growth. In this context, the aim of our study was to evaluate the level of different pools of circulating tumor cells and bone marrow progenitor cells in the blood of patients with breast cancer in the dynamics of neoadjuvant chemotherapy. Materials and methods: Patients (prospective study) with newly diagnosed invasive breast cancer in the age range 18–50 years and tumor volume of 2.0(> or =) cm, referred at the Tomsk Cancer Research Institute for treatment were included into the study. Eligibility criteria were as follows: the patient’s informed consent to participate in research; morphologically verified diagnosis of invasive carcinoma of non-specific type; luminal B-1, -2, triple negative (basal-like subtype included), HER2 positive breast cancer; T2-4N0-3M0; preserved menstrual function; satisfactory performance status (on a scale (< or =) ECOG 2). Exclusion Criteria were: other than luminal histological type of breast cancer, multiple primary cancer; chronic inflammatory diseases in the acute stage. Samples of venous blood taken from breast cancer patients before biopsy, after biopsy and after each subsequent course of neoadjuvant chemotherapy (NACHT) were served as a study material. The various pools of circulating tumor cells and bone-marrow derived progenitor cells were determined using monoclonal antibodies to EpCam, CD44, CD45, CD24, N-cadherin, CD34, CD133, CD202, VEGFR1 and CD90, labeled with different fluorochromes on flow cytometry BD FACSCanto ™ II. Results: The study showed that each succeeding course of NACHT increased blood levels of circulating tumor cells, and bone marrow progenitor cells with a phenotype characteristic of EPC (endothelial progenitor cells) (CD34 + CD45–VEGFR + CD133 + CD202 +) and MSC (mesenchymal progenitor stem cells fibroblasts) (CD34–CD90 + VEGFR1–CD45–CD202–). Influence of the NACHT on hematopoietic stem cells HSC (VEGFR1 + CD34 + CD45lowSD202–) and progenitor cells HPC – CD34 + CD45 + CD90–VEGFR1 + CD133 – was ambiguous. Conclusion: Neoadjuvant chemotherapy increases blood levels of circulating tumor cells, endothelial progenitor cells and mesenchymal stem cells progenitor fibroblasts, thus promoting the formation of premetastatic niche. The study was conducted with financial support from the Council for Grants of the President of the Russian Federation (Russia) for the state support of young philosophy doctors, agreements of SC 114.120.14.168-MD Russian Scientific Foundation (Russia), Grant No. 14-15-00318 (sample collection) and Russian Foundation for Basic Research (Russia), Grant No. 15-34-20864. We acknowledge support of this work by the Tomsk State University Competitiveness Improvement Program (Russia)

CYTOKINE GENE EXPRESSION ASSOCIATED WITH TUMOR AND PREMETASTATIC NICHES IN BREAST CANCER STROMA

The tumor niche in the microenvironment of the primary tumor is believed to reflect events occurring in the premetastatic niche. In our study, based on the assessment of the expression of cytokine genes associated with inflammation and invasive and metastatic phenotype of tumor cells in the tumor microenvironment, their contribution to the creation of favorable conditions for the development of tumor and metastases was described. Material and methods. The main clinical and pathological parameters in 12 patients with invasive breast carcinoma of non-specific type (IC NST) were studied. The expression of 13 genes encoding key cytokines and chemokines in the stroma of IC NST was assessed using the PALM laser microdissection system and real-time polymerase chain reaction. Results. A direct correlation between the size of the primary tumor and the expression levels of IL1b and CXCL8 genes was found. Furthermore, it was shown that the proliferative activity of tumor cells was inversely correlated with the expression CCL2 gene that attracted monocytes. The expression of IL6 and IL8 genes involves the differentiation of monocytes into M2 macrophages, which can stimulate tumor cell invasiveness. However, microenvironment cells were found not to express MST1, FGF7, EGF genes, protein products of which provide invasive and metastatic progression of tumor cells. In our study, no significant differences in gene expression levels between patients with and without lymph node metastases were found. Nevertheless, the use of the multivariate data analysis allowed us to reveal a close relationship between the studied parameters related to a high risk of lymph node metastasis. Conclusion. A wide range of cytokines involved in the development of inflammation, recruitment of monocytes, as well as secretion of factors promoting both tumor growth and lymphatic metastasis was identified. Similar events in premetastatic niche might contribute to the development macrometastasis