1,593 research outputs found
Weak magnetic fields in white dwarfs and their direct progenitors?
We have carried out a re-analysis of polarimetric data of central stars of
planetary nebulae, hot subdwarfs, and white dwarfs taken with FORS1 (FOcal
Reducer and low dispersion Spectrograph) on the VLT (Very Large Telescope), and
added a large number of new observations in order to increase the sample. A
careful analysis of the observations using only one wavelength calibration for
the polarimetrically analysed spectra and for all positions of the retarder
plate of the spectrograph is crucial in order to avoid spurious signals. We
find that the previous detections of magnetic fields in subdwarfs and central
stars could not be confirmed while about 10% of the observed white dwarfs have
magnetic fields at the kilogauss level.Comment: 6 pages, Proceedings of the 18th European White Dwarf Workshop, ASP
Conference Serie
Non-invasive and non-destructive measurements of confluence in cultured adherent cell lines
Many protocols used for measuring the growth of adherent monolayer cells in vitro are invasive, destructive and do not allow for the continued, undisturbed growth of cells within flasks. Protocols often use indirect methods for measuring proliferation. Microscopy techniques can analyse cell proliferation in a non-invasive or non-destructive manner but often use expensive equipment and software algorithms. In this method images of cells within flasks are captured by photographing under a standard inverted phase contract light microscope using a digital camera with a camera lens adaptor. Images are analysed for confluence using ImageJ freeware resulting in a measure of confluence known as an Area Fraction (AF) output. An example of the AF method in use on OVCAR8 and UPN251 cell lines is included.
•Measurements of confluence from growing adherent cell lines in cell culture flasks is obtained in a non-invasive, non-destructive, label-free manner.
•The technique is quick, affordable and eliminates sample manipulation.
•The technique provides an objective, consistent measure of when cells reach confluence and is highly correlated to manual counting with a haemocytometer. The average correlation co-efficient from a Spearman correlation (n = 3) was 0.99 ± 0.008 for OVCAR8 (p = 0.01) and 0.99 ± 0.01 for UPN251 (p = 0.01) cell lines
A New Monte Carlo Algorithm for Protein Folding
We demonstrate that the recently proposed pruned-enriched Rosenbluth method
(P. Grassberger, Phys. Rev. E 56 (1997) 3682) leads to extremely efficient
algorithms for the folding of simple model proteins. We test them on several
models for lattice heteropolymers, and compare to published Monte Carlo
studies. In all cases our algorithms are faster than all previous ones, and in
several cases we find new minimal energy states. In addition to ground states,
our algorithms give estimates for the partition sum at finite temperatures.Comment: 4 pages, Latex incl. 3 eps-figs., submitted to Phys. Rev. Lett.,
revised version with changes in the tex
Aberrant phenotype in human endothelial cells of diabetic origin: Implications for saphenous vein graft failure?
Type 2 diabetes (T2DM) confers increased risk of endothelial dysfunction, coronary heart disease, and vulnerability to vein graft failure after bypass grafting, despite glycaemic control. This study explored the concept that endothelial cells (EC) cultured from T2DM and nondiabetic (ND) patients are phenotypically and functionally distinct. Cultured human saphenous vein-(SV-) EC were compared between T2DM and ND patients in parallel. Proliferation, migration, and in vitro angiogenesis assays were performed; western blotting was used to quantify phosphorylation of Akt, ERK, and eNOS. The ability of diabetic stimuli (hyperglycaemia, TNF-α, and palmitate) to modulate angiogenic potential of ND-EC was also explored. T2DM-EC displayed reduced migration (30%) and angiogenesis (40%) compared with ND-EC and a modest, nonsignificant trend to reduced proliferation. Significant inhibition of Akt and eNOS, but not ERK phosphorylation, was observed in T2DM cells. Hyperglycaemia did not modify ND-EC function, but TNF-α and palmitate significantly reduced angiogenic capacity (by 27% and 43%, resp.), effects mimicked by Akt inhibition. Aberrancies of EC function may help to explain the increased risk of SV graft failure in T2DM patients. This study highlights the importance of other potentially contributing factors in addition to hyperglycaemia that may inflict injury and long-term dysfunction to the homeostatic capacity of the endothelium
Visions, Values, and Videos: Revisiting Envisionings in Service of UbiComp Design for the Home
UbiComp has been envisioned to bring about a future dominated by calm
computing technologies making our everyday lives ever more convenient. Yet the
same vision has also attracted criticism for encouraging a solitary and passive
lifestyle. The aim of this paper is to explore and elaborate these tensions
further by examining the human values surrounding future domestic UbiComp
solutions. Drawing on envisioning and contravisioning, we probe members of the
public (N=28) through the presentation and focus group discussion of two
contrasting animated video scenarios, where one is inspired by "calm" and the
other by "engaging" visions of future UbiComp technology. By analysing the
reasoning of our participants, we identify and elaborate a number of relevant
values involved in balancing the two perspectives. In conclusion, we articulate
practically applicable takeaways in the form of a set of key design questions
and challenges.Comment: DIS'20, July 6-10, 2020, Eindhoven, Netherland
Discovery of magnetic fields in central stars of planetary nebulae
For the first time we have directly detected magnetic fields in central stars
of planetary nebulae by means of spectro-polarimetry with FORS1 at the VLT. In
all four objects of our sample we found kilogauss magnetic fields, in NGC1360
and LSS1362 with very high significance, while in EGB5 and Abell36 the
existence of a magnetic field is probable but with less certainty. This
discovery supports the hypothesis that the non-spherical symmetry of most
planetary nebulae is caused by magnetic fields in AGB stars. Our high discovery
rate demands mechanisms to prevent full conservation of magnetic flux during
the transition to white dwarfs.Comment: 8 pages, 4 figures, Accepted for Publication by Astronomy &
Astrophysics See also press release by A&A on their homepage
www.edpsiences.or
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