Microchip electrophoresis bioanalytical applications

Microchip electrophoresis (MCE) is a novel analytical technique resulting from miniaturization of capillary electrophoresis (CE) to a planar microfabricated separation device. The consequences of the transfer of CE to MCE in terms of benefits and drawbacks have been identified and commented. The strategies developed to overcome the unfavourable features of the chip with respect to the capillary are briefly described. A method for simultaneous separation of catecholamines and their cationic metabolites has been developed on the microchip. The addition of three modifiers was required to resolve all analytes. The sensitivity of on-chip amperometric detection has been improved by employing an enzyme-catalyzed reaction on the amperometric electrode, as well as by using a carbon nanotube-modified electrode. The developed analytical methodology has been successfully applied for a direct on-chip determination of catecholamines and their metabolites in a mouse brain homogenate. The feasibility of performing affinity measurements as well as isoelectric focusing on the microchip has been demonstrated and available applications of these two electrophoretic modes on a chip have been reviewed. A commercial Shimadzu microchip station has for the first time been applied for high-throughput microchip isoelectric focusing of therapeutic proteins and obtained results have been compared to conventional capillary isoelectric focusing

Results and observations from the reconstruction of continuous time series of mortality by cause of death

Though many countries have been collecting cause-of-death information from death certificates for more than a century, these valuable data sources remain underexplored. This is certainly due in part to the frequent revisions of the International Classification of Diseases (ICD), which have caused breaks in data continuity. This paper describes the methodological background and the results of the transition between the eighth and the ninth revision of ICD for the territory of former West Germany. Using practical examples, we examine the difficulties encountered and discuss the solutions applied.causes of death, mortality, West Germany

Reconstruction of continuous series of mortality by cause of death in West Germany for the years 1968-1997

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GEOPOL®. The innovated environment friendly inorganic binder system

This paper deals with the complete technology of inorganic geopolymer binder system GEOPOL® which is a result of long term research and development. The objective of this paper is to provide a theoretical and practical overview of the GEOPOL® binder system and introduce possible ways of moulds and cores production in foundries. GEOPOL® is a unique inorganic binder system, which is needed and welcomed in terms of the environment, the work environment, and the sustainable resources. The GEOPOL® technology is currently used in the foundries for three basic production processes/technologies: (1) for self-hardening moulding mixtures, (2) sand mixtures hardened by gaseous carbon dioxide and (3) the hot box technology with hot air hardening. The GEOPOL® technology not only solves the binder system and the ways of hardening, but also deals with the entire foundry production process. Low emissions produced during mixing of sand, moulding, handling, and pouring bring a relatively significant improvement of work conditions in foundries (no VOCs). A high percentage of the reclaim sand can be used again for the preparation of the moulding mixture.Web of Science19111610

Concept of leak tightness monitoring at underground gas storage

The most important part of the operation of each underground gas storage facility is the implementation of a regular monitoring system for leak tightness monitoring. This article deals with the design of a proposal leak tightness monitoring concept in underground gas storage in porous reservoirs (depleted gas/oil reservoirs, aquifer reservoirs). This concept interconnects all commonly used and established leak tightness monitoring methodologies and evaluates them in time with comparison with historical results with reference to possible solutions or implementation of remedial measure

Concept of e-shop

Bakalářská práce „Návrh elektronického obchodu“ se zabývá konceptuálním modelováním dat aplikovaných na konkrétním návrhu e-shopu společnosti. Teoretická východiska zahrnují uvedení do problematiky konceptuálního modelování a osvětlují právní aspekty důležité pro elektronické obchodování. Praktická část charakterizuje vybranou společnost a poukazuje na výhody implementace návrhu, kterým je konceptuální model složený z entit, relací a jejich zakreslení do E-R diagramů.Bachelor thesis „Concept of e-shop“ deals with conceptual data modelling that is apllied to specific concept of company’s e-shop. Theoretical basis includes introduction to conceptual data modelling and legal aspects important for e-commerce. Practical part describes chosen company and points to advantages of concept implementation. Conceptual model consists of entities, relationships and their presentation in E-R diagrams.

Wine Farmstead in Jaroslavice

Bakalářská práce řeší území bývalé cihelny v Jaroslavicích. Novou náplní území je rodinné vinařství. Pozemek je významně poznamenán původní náplní a tento fakt výrazně určuje nové využití jednotlivých částí pozemku. Objekt vinařství je navrhován v návaznosti na komunikaci od obce Jaroslavice. Vytváří předěl mezi severní a jižní částí pozemku, které mají odlišné funkce. Objekt svým tvarem reaguje na terén do něhož je vtlačen. Pod jednou střechou ukrývá dva odlišné provozy, které se svou podstatou propisují do fasády a utváří tak charakter domu.This bachelor thesis analyzes an area of brickfield in Jaroslavice. The new content of the area is a family vineyard. The original purpose is significant all around which strongly affects the new use of every part of the area. The object of the vineyard is designed in the connection of comunication from the village Jaroslavice. It divides the area into the north and south part which both have different purpose. The object with its shape reacts to the terrain it is dug into. Under one roof you can find two divergent services which with its principles reflect on the fasade and so make the character of the house.

Non-traditional foods containing probiotic bacteria

Probiotické mikroorganismy jsou obsaženy kromě běžných mléčných produktů také v netradičních potravinách konzumovaných zejména v asijských zemích. V práci je uveden přehled vybraných méně obvyklých potravin s obsahem probiotických mikroorganismů, které nejsou řazeny mezi mléčné fermentované výrobky. Jsou diskutovány pozitivní účinky probiotických potravin na zdravotní stav člověka. Experimentální část práce se zabývá identifikací probiotických mikroorganismů přítomných ve vybraném probiotickém výrobku asijského původu. DNA byla z produktu izolována metodou fenolové extrakce a za využití magnetických částic. Přítomnost probiotických MO byla zjišťována za využití metody polymerázové řetězové reakce v reálném čase (qPCR).Probiotic microorganisms are contained in common dairy products as well in unconventional probiotic products consumed especially in Asian countries. In the thesis is shown overview of chosen less common foodstuffs containing probiotic microorganisms, which no belong to fermented dairy products. In the thesis are discussed positive health benefits of probiotics foodstuffs on the host. The experimental part deals with the identification of probiotic microorganisms, which are contained in chosen probiotic product from Asia. DNA was isolated from the product by use phenol extraction and using magnetic particles. Probiotic microorganisms in chosen product were detected using method real-time polymerase chain reaction (qPCR).