From book to stage to screen: semiotic transformations of Gothic horror genre conventions

This paper adopts a multimodal social semiotic approach for exploring the semiotic changes involved in the transformation of a novel into stage and screen productions. It examines how semiotic resources are deployed in each medium through elements of mise-en-scène, such as speech, music, sound, lighting, props, staging, and cinematographic techniques, and the viewing perspectives that are thus established for audiences. The genre of Gothic horror is selected for this purpose, given how this form of performance has transfixed audiences for centuries and has been adapted for both the stage and the screen. In order to demonstrate how each performance medium has produced its own unique set of foregrounding devices to enthral and captivate audiences, a comparative analysis of excerpts from the novel The Woman in Black by Susan Hill, a videotaped theatrical performance, and the 1989 British television film of the same name is undertaken. The paper discusses the implications of the multimodal semiotic approach for developing a better understanding of the semiotic transformations that horror genre conventions undergo in different media and the viewership positions that are thus re-drawn for audiences. The paper concludes with a view of multimodal recontextualisation processes which form the underlying basis of human sociocultural life

Mechanisms of nuclear content loading to exosomes.

Exosome cargoes are highly varied and include proteins, small RNAs, and genomic DNA (gDNA). The presence of gDNA suggests that different intracellular compartments contribute to exosome loading, resulting in distinct exosome subpopulations. However, the loading of gDNA and other nuclear contents into exosomes (nExo) remains poorly understood. Here, we identify the relationship between cancer cell micronuclei (MN), which are markers of genomic instability, and nExo formation. Imaging flow cytometry analyses reveal that 10% of exosomes derived from cancer cells and <1% of exosomes derived from blood and ascites from patients with ovarian cancer carry nuclear contents. Treatment with genotoxic drugs resulted in increased MN and nExos both in vitro and in vivo. We observed that multivesicular body precursors and exosomal markers, such as the tetraspanins, directly interact with MN. Collectively, this work provides new insights related to nExos, which have implications for cancer biomarker development

The extent of and factors associated with self-reported overdose and self-reported receipt of naloxone among people who inject drugs (PWID) in England, Wales and Northern Ireland.

BACKGROUND: Overdose is a major cause of death among PWID, and for opioid overdoses naloxone administration can reduce harm. However, globally there is limited national level data on the extent of non-fatal overdose and naloxone uptake. The first national level data on the extent of self-reported overdose and self-reported receipt of naloxone among UK PWID, providing a baseline to monitor the impact of the recent policy change regarding naloxone availability, is presented. METHODS: Data on self-reported overdose and receipt of naloxone during the preceding year for 2013-2014 from a national survey of PWID was analysed. Participants who reported injecting during the preceding year were included. RESULTS: Participants (3850) were predominantly male (75%); mean age was 36 years. The most commonly injected drugs were: heroin (91%), crack (45%) and amphetamine (29%). 15% (591) reported overdosing during the preceding year. There were no differences in the proportion reporting overdose by age or gender, but overdose was more common among those who: injected multiple drugs; recently ceased addiction treatment; injected with used needles/syringes; ever had transactional sex; had used a sexual health clinic or emergency department and lived in Wales or Northern Ireland. Among those reporting an overdose during the preceding year, a third reported two to four overdoses and 7.5% five or more overdoses; half reported receiving naloxone. Those reporting naloxone receipt in the preceding year were more likely to: live in Wales or Northern Ireland; ever received used needles/syringes; ever been imprisoned; and less likely to have injected two drug types. CONCLUSION: These data provide a baseline for monitoring the impact of the 2015 UK policy change to improve take-home naloxone access. Interventions tackling overdose should promote naloxone awareness and access, and target those who; are poly-drug injectors, have ceased treatment, share needles/syringes and whose drug use links to sexual activity

Analysis of social attitude to the new end use of recycled water for household laundry in Australia by the regression models

Recycled water for household laundry can be regarded as a promising strategy to alleviate the current demand on scarce water supplies. Public acceptability becomes fairly important to ensure the successful establishment and development of this new end use. To address the issue, this study conducted social surveys in two locations of Australia, Port Macquarie and Melbourne, where respondents were asked 17 questions. The regression models provide conclusions about which characteristics are more likely to lead to the acceptance of recycled water from society. Three attitudinal variables (RWAlterDW, Attitude and Cost) and three psychological variables (Odour, Reading and SmallUnit) were found to be the key driving forces behind domestic water reuse behaviour. These findings could drive the future research direction to achieve better public perception of this new end use of recycled water. © 2013 Elsevier Ltd

Vision and perception of community on the use of recycled water for household laundry: A case study in Australia

This study investigates the community perception of household laundry as a new end use of recycled water in three different locations of Australia through a face to face questionnaire survey (n=478). The study areas were selected based on three categories of (1) non-user, (2) perspective user and (3) current user of recycled water. The survey results indicate that significantly higher number (70%) of the respondents supported the use of recycled water for washing machines (χ2=527.40, df=3; p=0.000). Significant positive correlation between the overall support for the new end use and the willingness of the respondents to use recycled water for washing machine was observed among all users groups (r=0.43, p=0.000). However, they had major concerns regarding the effects of recycled water on the aesthetic appearance of cloth, cloth durability, machine durability, odour of the recycled water and cost along with the health issues. The perspective user group had comparatively more reservations and concerns about the effects of recycled water on washing machines than the non-users and the current users (χ2=52.73, df=6; p=0.000). Overall, community from all three study areas are willing to welcome this new end use as long as all their major concerns are addressed and safety is assured. © 2013 Elsevier B.V

Addressing international students on Australian and Chinese university webpages: A comparative study

This article investigates the discursive construction of international students on Australian and Chinese university webpages that are directed at international students. The international student theme webpages of three Australian and three Chinese universities were examined in relation to how verbal and visual semiotic resources were co-deployed on the webpages to address international students in the climate of neoliberal thinking in higher education. The tools used for analysis and interpretation were informed by socio-semiotic approaches to multimodal discourse analysis, international education discourses and communication accommodation theories. It was found that the Australian and Chinese university webpages differed in several salient ways to the effect that international students were portrayed as agentive and informed individuals to explore a study abroad experience at the Australian universities versus being explicitly guided through their study abroad at the Chinese universities. These results were compared and interpreted as reflecting each country’s conception of transnational education. The article concludes with a summary of the impacts of cultural and intercultural factors and neoliberal thinking in higher education on multimodal representations of international students on university webpages and directions for further research

A comprehensive framework for the assessment of new end uses in recycled water schemes

Nowadays, recycled water has provided sufficient flexibility to satisfy short-term freshwater needs and increase the reliability of long-term water supplies in many water scarce areas, which becomes an essential component of integrated water resources management. However, the current applications of recycled water are still quite limited that are mainly associated with non-potable purposes such as irrigation, industrial uses, toilet flushing and car washing. There is a large potential to exploit and develop new end uses of recycled water in both urban and rural areas. This can greatly contribute to freshwater savings, wastewater reduction and water sustainability. Consequently, the paper identified the potentials for the development of three recycled water new end uses, household laundry, livestock feeding and servicing, and swimming pool, in future water use market. To validate the strengths of these new applications, a conceptual decision analytic framework was proposed. This can be able to facilitate the optional management strategy selection process and thereafter provide guidance on the future end use studies within a larger context of the community, processes, and models in decision-making. Moreover, as complex evaluation criteria were selected and taken into account to narrow down the multiple management alternatives, the methodology can successfully add transparency, objectivity and comprehensiveness to the assessment. Meanwhile, the proposed approach could also allow flexibility to adapt to particular circumstances of each case under study. © 2013 Elsevier B.V

Herschel/PACS observations of the host galaxy of GRB 031203

We present Herschel/PACS observations of the nearby (z = 0.1055) dwarf galaxy that has hosted the long gamma-ray burst (LGRB) 031203. Using the PACS data, we have been able to place constraints on the dust temperature, dust mass, total infrared (IR) luminosity and IR-derived star formation rate (SFR) for this object. We find that the GRB host galaxy (GRBH) 031203 has a total IR luminosity of 3 × 1010 L⊙ placing it in the regime of the IR-luminous galaxy population. Its dust temperature and specific SFR are comparable to that of many high-redshift (z = 0.3–2.5) IR-detected GRB hosts (Tdust > 40 K; sSFR > 10 Gyr−1); however, its dust-to-stellar mass ratio is lower than what is commonly seen in IR-luminous galaxies. Our results suggest that GRBH 031203 is undergoing a strong starburst episode and its dust properties are different to those of local dwarf galaxies within the same metallicity and stellar mass range. Furthermore, our measurements place it in a distinct class to the well-studied nearby host of GRB 980425 (z = 0.0085), confirming the notion that GRB host galaxies can span a large range in properties even at similar cosmological epochs, making LGRBs an ideal tool in selecting samples of star-forming galaxies up to high redshift