Homogénéisation des signaux isotopiques, 18O et 3H, dans un système hydrologique de haute montagne : la Vallée d'Aoste (Italie)

L'analyse de l'origine et de la dynamique des écoulements souterrains dans l'aquifère du milieu alluvial de la plaine d'Aoste (Italie) a été menée en étudiant les teneurs isotopiques (18O et 3H) des eaux sur l'ensemble du système hydrologique. La démarche a consisté en un suivi isotopique des précipitations pluvio-neigeuses, des sources de versants, du réseau de surface et de l'aquifère alluvial, complété par des analyses ponctuelles concernant l'horizon superficiel des glaciers et les sous-écoulements glaciaires. Le signal d'entrée en 18O porté par les précipitations présente une grande variabilité liée au contexte orographique (effet d'écran) et climatique (pluie ou neige). Celle-ci est mise en évidence par les changements saisonniers du gradient 18O/altitude. En moyenne annuelle (1994), ce gradient établi en Vallée d'Aoste à partir de mesures sur les précipitations récoltées entre 300 et 3500 m d'altitude (sur huit stations) est de - 0.18 ± 0.02 ‰ pour 100 m, avec un écart-type (s) des valeurs de ± 4,8 ‰. L'ensemble des résultats en 18O montre au cours du cycle hydrologique, un amortissement à la fois important et progressif du signal d'entrée fourni par les précipitations. Cet amortissement, de l'ordre de 30 fois dans l'aquifère alluvial (sigma=± 0.15 ‰), est particulièrement sensible au niveau de la glace et des sous-écoulements glaciaires. En ce qui concerne les résultats en 3H, les teneurs mesurées dans les précipitations se révèlent être étroitement liées avec l'origine et la trajectoire des masses d'air humide. Par ailleurs, on note une bonne concordance entre les temps de séjour des eaux dans l'aquifère alluvial calculés à partir des valeurs en 18O et ceux fournis par le 3H.The hydrogeological study of the alluvial aquifer of the Aosta plain (Italy) is chosen as one of the most appropriate examples representative of the Italian-French-Swiss Alps. This study has been carried out using two environmental isotopes (18O and 3H) for groundwater samples taken from this aquifer in order to shed light on the origin of water and the hydrodynamic characteristics of the aquifer.The surface area of this aquifer is 70 km_ and mean elevation ranges between 400 and 700 m (asl). This surface area constitutes a part of the watershed area that amounts 2400 km_ with a mean elevation of 2200 m (asl). The watershed possesses several mountain peaks, Mt. Blanc, Mt. Cervin, Mt. Grand Paradis, the elevation of which rise to over 4000 m.The input signal, quantitative and qualitative as well, provided by the precipitation shows a wide variability linked to both orographic (screen effect) and climatic (rain or snow) influences. This variability is marked by differences in the 18O/altitude gradient, which is seasonally dependent. A unique and linear relation is observed during spring and summer ; in autumn and winter results show an important gradient up to 2000 m but a less important one for higher altitudes.The mean annual (1994) gradient of - 0.18 ± 0.02 ‰ for 100 m is determined in Aosta valley for altitudes between 300 and 3500 m, and the standard deviation (sigma) of the mean volume-weighted value for precipitation is ± 4.8 ‰. Furthermore, the 18O values measured in the alluvial aquifer have shown a very low dispersion (sigma=± 0.15 ‰), which corresponds to a buffering effect of a factor 30. These results evidenced, thus, an homogenization process within the different water bodies.To analyze this homogenization process, the different components of the hydrologic system have been studied, i.e. the monitoring of the springs and runoff waters, with complementary local measurements of the upper part of ice cores and the glacier underflows. Using standard deviations (sigma) as criteria for the homogenization process, one can see a large and progressive decrease along the hydrological cycle, with a special amplitude in ice cores and glacier underflows : precipitation (± 4.8 ‰), ice core (± 0.8 ‰), glacier underflow (± 0.10 ‰), spring (± 0.15 ‰), surface runoff network (± 0.3 ‰), alluvial aquifer (± 0.15 ‰).In ice cores, the different process : freezing and thawing, compression, and vapor / liquid / solid exchanges have caused the 18O homogenization. Dealing with the springs on the slopes of the valley, the preferential flow paths linked to the fracturation lead to a mixing of waters and hence to a very buffered outflow signal. The combined effect of all these explains the stability and the uniformity of the data found in the alluvial water body.As far as the 3H concentrations are concerned, the values measured are tightly linked with the origin and the transport of humid air masses. For closely spaced stations, a significant difference in the input signal has been correlated to various climatic influences. Using 3H data from the Thonon-les-Bains station (included in the WMO-IAEA observation network) as input, the calculated transit times of 2.5 to 7.5 years are very close to those calculated with 18O, i.e. 4 to 7 years

A Methodological Framework for the Evaluation of Syndromic Surveillance Systems: A Case Study of England

Background: Syndromic surveillance complements traditional public health surveillance by collecting and analysing health indicators in near real time. The rationale of syndromic surveillance is that it may detect health threats faster than traditional surveillance systems permitting more timely, and hence potentially more effective public health action. The effectiveness of syndromic surveillance largely relies on the methods used to detect aberrations. Very few studies have evaluated the performance of syndromic surveillance systems and consequently little is known about the types of events that such systems can and cannot detect. Methods: We introduce a framework for the evaluation of syndromic surveillance systems that can be used in any setting based upon the use of simulated scenarios. For a range of scenarios this allows the time and probability of to be determined and uncertainty is fully incorporated. In addition, we demonstrate how such a framework can model the benefits of increases in the number of centres reporting syndromic data and also determine the minimum size of outbreaks that can or cannot be detected. Here, we demonstrate its utility using simulations of national influenza outbreaks and localised outbreaks of cryptosporidiosis. Results: Influenza outbreaks are consistently detected with larger outbreaks being detected in a more timely manner. Small cryptosporidiosis outbreaks (<1000 symptomatic individuals) are unlikely to be detected. We also demonstrate the advantages of having multiple syndromic data streams (e.g. emergency attendance data, telephone helpline data, general practice consultation data) as different streams are able to detect different types outbreaks with different efficacy (e.g. emergency attendance data are useful for the detection of pandemic influenza but not for outbreaks of cryptosporidiosis). We also highlight that for any one disease, the utility of data streams may vary geographically, and that the detection ability of syndromic surveillance varies seasonally (e.g. an influenza outbreak starting in July is detected sooner than one starting later in the year). We argue that our framework constitutes a useful tool for public health emergency preparedness in multiple settings. Conclusions: The proposed framework allows the exhaustive evaluation of any syndromic surveillance system and constitutes a useful tool for emergency preparedness and response

Respiratory viruses in children hospitalized for acute lower respiratory tract infection in Ghana

<p>Abstract</p> <p>Background</p> <p>Acute respiratory tract infections are one of the major causes of morbidity and mortality among young children in developing countries. Information on the viral aetiology of acute respiratory infections in developing countries is very limited. The study was done to identify viruses associated with acute lower respiratory tract infection among children less than 5 years.</p> <p>Method</p> <p>Nasopharyngeal samples and blood cultures were collected from children less than 5 years who have been hospitalized for acute lower respiratory tract infection. Viruses and bacteria were identified using Reverse Transcriptase Real-Time Polymerase Chain Reaction and conventional biochemical techniques.</p> <p>Results</p> <p>Out of 128 patients recruited, 33(25.88%%, 95%CI: 18.5% to 34.2%) were positive for one or more viruses. Respiratory Syncytial Virus (RSV) was detected in 18(14.1%, 95%CI: 8.5% to 21.3%) patients followed by Adenoviruses (AdV) in 13(10.2%, 95%CI: 5.5% to 16.7%), Parainfluenza (PIV type: 1, 2, 3) in 4(3.1%, 95%CI: 0.9% to 7.8%) and influenza B viruses in 1(0.8%, 95%CI: 0.0 to 4.3). Concomitant viral and bacterial co-infection occurred in two patients. There were no detectable significant differences in the clinical signs, symptoms and severity for the various pathogens isolated. A total of 61.1% (22/36) of positive viruses were detected during the rainy season and Respiratory Syncytial Virus was the most predominant.</p> <p>Conclusion</p> <p>The study has demonstrated an important burden of respiratory viruses as major causes of childhood acute respiratory infection in a tertiary health institution in Ghana. The data addresses a need for more studies on viral associated respiratory tract infection.</p

Tracking virus outbreaks in the twenty-first century

Emerging viruses have the potential to impose substantial mortality, morbidity and economic burdens on human populations. Tracking the spread of infectious diseases to assist in their control has traditionally relied on the analysis of case data gathered as the outbreak proceeds. Here, we describe how many of the key questions in infectious disease epidemiology, from the initial detection and characterization of outbreak viruses, to transmission chain tracking and outbreak mapping, can now be much more accurately addressed using recent advances in virus sequencing and phylogenetics. We highlight the utility of this approach with the hypothetical outbreak of an unknown pathogen, 'Disease X', suggested by the World Health Organization to be a potential cause of a future major epidemic. We also outline the requirements and challenges, including the need for flexible platforms that generate sequence data in real-time, and for these data to be shared as widely and openly as possible

GMOs in animal agriculture: time to consider both costs and benefits in regulatory evaluations

In 2012, genetically engineered (GE) crops were grown by 17.3 million farmers on over 170 million hectares. Over 70% of harvested GE biomass is fed to food producing animals, making them the major consumers of GE crops for the past 15 plus years. Prior to commercialization, GE crops go through an extensive regulatory evaluation. Over one hundred regulatory submissions have shown compositional equivalence, and comparable levels of safety, between GE crops and their conventional counterparts. One component of regulatory compliance is whole GE food/feed animal feeding studies. Both regulatory studies and independent peer-reviewed studies have shown that GE crops can be safely used in animal feed, and rDNA fragments have never been detected in products (e.g. milk, meat, eggs) derived from animals that consumed GE feed. Despite the fact that the scientific weight of evidence from these hundreds of studies have not revealed unique risks associated with GE feed, some groups are calling for more animal feeding studies, including long-term rodent studies and studies in target livestock species for the approval of GE crops. It is an opportune time to review the results of such studies as have been done to date to evaluate the value of the additional information obtained. Requiring long-term and target animal feeding studies would sharply increase regulatory compliance costs and prolong the regulatory process associated with the commercialization of GE crops. Such costs may impede the development of feed crops with enhanced nutritional characteristics and durability, particularly in the local varieties in small and poor developing countries. More generally it is time for regulatory evaluations to more explicitly consider both the reasonable and unique risks and benefits associated with the use of both GE plants and animals in agricultural systems, and weigh them against those associated with existing systems, and those of regulatory inaction. This would represent a shift away from a GE evaluation process that currently focuses only on risk assessment and identifying ever diminishing marginal hazards, to a regulatory approach that more objectively evaluates and communicates the likely impact of approving a new GE plant or animal on agricultural production systems

Aquifer-river exchanges in an alpine valley: Quantification and modelling (Valee D'Aoste, Italy)

A mathematical model is applied to the Aoste Valley (Italy), which is a good example of the hydrological workings of alluvial rock basin aquifers in mountainous regions. The course of the Dorea Baltea follows the valley, which between the altitudes of 500 and 600 m is dominated by summits reaching more than 3000 m. The lacustrine- and fluviatile-type sedimentary deposits are accompanied by several alluvial cones that, due to their varying nature, confer very diverse hydrodynamic characteristics to the land. In this particular context, the source and drainage of the Dorea control the hydrodynamics of the aquifers and influence the spatial dispersion of the physicochemical properties of the groundwater. The model also led to quantify and determine the zones and types of exchanges with the river. Simulation of lowering the water table in the river showed the variable sensitivity of the water table piezometry with various spatial impacts. Finally, the stream-aquifer impact is highlighted by the spatial evolution of the sulphate contents coming from deep lateral sources. Taking into account the stream-aquifer exchanges in an underground-flow mathematical model gives a better understanding of the workings of the valley aquifers and thus their management, especially for the development of watercourses in mountainous regions

Quantitative histomorphometric analysis of halved iliac crest bone biopsies yield comparable ROD diagnosis as full 7.5mm wide samples

BACKGROUND AND OBJECTIVES: Histomorphometric analysis of a transiliac bone biopsy is the gold standard for the diagnosis of renal osteodystrophy (ROD). This procedure is costly, invasive and usually performed with a trephine with an internal diameter of 7.5 mm. Our objective was to evaluate the accuracy of ROD diagnosis on halved histological bone sections to determine if they are comparable to the standard 7.5 mm samples. DESIGN: We included 68 bone biopsies performed in CKD patients for diagnostic purposes with a 7.5 mm diameter trephine. Quantitative histomorphometric analysis of the whole bone samples was performed including assessment of bone mineralization, turnover and volume. Each histological section (representing the whole 7.5 mm diameter biopsy) was then divided lengthwise in two hemisections (representing the 3.5 mm diameter biopsy). Histomorphometric analysis was repeated this time on the two hemibiopsies for each sample, blinded from initial results. Diagnoses were classified as osteitis fibrosa, adynamic bone disease, mixed uremic bone disease, osteomalacia or other. Correlations between the whole sample and the hemibiopsies for each parameter were studied. Concordance between the various bone parameters and final ROD diagnosis obtained from the whole section versus the two hemi sections was evaluated. RESULTS: Highly significant correlations were found between parameters measured on the whole section and the corresponding hemisections, with r coefficient of 0.98 for osteoid surface and thickness and bone formation rate, 0.97 for osteoclast surface, and 0.96 for bone volume (p \textless 0.001). Final diagnosis was in full accordance between the whole biopsy and the two corresponding hemi-biopsies in 91% of cases. CONCLUSIONS: Accurate diagnosis of ROD type was obtained by evaluation of bone surface areas of 3 mm diameter. These data suggest that small invasive bone biopsies might provide accurate ROD diagnostics while decreasing both invasiveness and cost of the procedure

Complexité des circulations dans l'aquifère alluvial de la plaine d'Aoste (Italie) : mise en évidence par l'hydrogéochimie [Complexity of the groundwater flow in the alluvial aquifer of the Aosta plain (Italy): Study with hydrogeochemistry]

The Aosta plain like any intra-ridge alluvial valley, is it hydrogeological system where a multilayer detritic aquifer is recharged by several subwatersheds. In such valleys the preservation of the chemical (mass transfert) and hydraulic (pressure transfert) signals from upstream (input) to downstream (output) can no longer be accepted. Considering the fact that vertical heterogeneities engender hydraulic and chemical discontinuities, in Aosta plain the occurrence of lateral discontinuities in the same sedimentary body. ill hydraulic continuity, should also be taken into account. The geochemical evolution is the result of multiple natural and anthropogenic influences which mark Out the groundwater flows. In the Aosta plain these mechanisms have been set up in space and time by the means of water chemistry and environmental isotopes