Compiled and Edited Tennessee Laws Pertaining to Animals

Foreward: The editors have designed this book principally to serve as an edited collection of Tennessee statutes relating to animals. Because these statutes were collected and edited, the collection is necessarily incomplete. The criteria and process used by the editors in selecting statutes of interest to our target audiences were thoughtfully conceived. However, the choices they made may differ from those that you would make. We welcome feedback from you—including suggestions for future editions—as to how we can better edit this resource to serve your needs. Moreover, especially because this book is a selective collection of statutory law available on a specific date, readers should not rely on this book as a source of legal advice. The legislature of the State of Tennessee can and will add, modify, and repeal laws in every legislative session. Additionally, courts have the opportunity to interpret and add to the law with each new case that properly comes before them. Finally, ordinances and other rules serve to make these laws applicable and enforceable on a local level. The interaction of these sources of regulation is complex and the rules of conduct that result from that interaction are best identified and explained by attorneys licensed to practice in the State of Tennessee that are familiar with the applicable statutes, court cases, ordinances, and other rules. We urge you to seek counsel from these legal advisors in interpreting and using the statutes presented in this book; a mere reading of the statutes is not enough to ensure complete understanding

The Arp2/3 Complex, UNC-115/abLIM, and UNC-34/Enabled Regulate Axon Guidance and Growth Cone Filopodia Formation in Caenorhabditis Elegans

Background While many molecules involved in axon guidance have been identified, the cellular and molecular mechanisms by which these molecules regulate growth cone morphology during axon outgrowth remain to be elucidated. The actin cytoskeleton of the growth cone underlies the formation of lamellipodia and filopodia that control growth cone outgrowth and guidance. The role of the Arp2/3 complex in growth cone filopodia formation has been controversial, and other mechanisms of growth cone filopodia formation remain to be described. Results Here we show that mutations in genes encoding the Arp2/3 complex (arx genes) caused defects in axon guidance. Analysis of developing growth cones in vivo showed that arx mutants displayed defects in filopodia and reduced growth cone size. Time-lapse analysis of growth cones in living animals indicated that arx mutants affected the rate of growth cone filopodia formation but not filopodia stability or length. Two other actin modulatory proteins, UNC-115/abLIM and UNC-34/Enabled, that had been shown previously to affect axon guidance had overlapping roles with Arp2/3 in axon guidance and also affected the rate of filopodia initiation but not stability or length. Conclusion Our results indicate that the Arp2/3 complex is required cell-autonomously for axon guidance and growth cone filopodia initiation. Furthermore, they show that two other actin-binding proteins, UNC-115/abLIM and UNC-34/Enabled, also control growth cone filopodia formation, possibly in parallel to Arp2/3. These studies indicate that, in vivo, multiple actin modulatory pathways including the Arp2/3 complex contribute to growth cone filopodia formation during growth cone outgrowth

03. Notch Signaling Plays a Key Role in Regulating Adult Stem Cell Osteogenic Differentiation

Human adipose-derived stem cells (hASCs) are an easily accessible type of multipotent stem cell that boasts immense potential as a cell source for personalized regenerative medicine. Given their tremendous clinical potential, our research strives to better understand hASCs and their potential use in combating degenerative bone diseases such as osteoporosis and trauma related degradation. We are specifically interested in deciphering the role of the highly conserved Notch signaling pathway and how it might be manipulated to enhance bone differentiation and regeneration. The Notch signaling pathway is a cell-to-cell contact dependent pathway that is involved in cellular homeostasis, stem cell differentiation, and cell fate determination. Notch1 and Notch3 are two receptors in the Notch signaling pathway that play a critical role in osteogenesis and self-renewal. To better understand the role of these two receptors, siRNA knockdowns are performed. Small interfering RNAs lead to the degradation of a significant amount of a targeted mRNA transcript, and in turn results in a significant decrease in protein expression. The literature indicates that a decrease in Notch1 expression leads to a decrease in osteogenic differentiation. Given data in the literature for other types of cells, we expect to confirm that the decrease in Notch1 leads to a decrease in hASC osteogenesis while the decrease in Notch3 may lead to enhanced differentiation. Identifying the role of each receptor will aid in identifying potential therapeutic targets for treating bone degeneration and loss in the future

The Arp2/3 complex, UNC-115/abLIM, and UNC-34/Enabled regulate axon guidance and growth cone filopodia formation in Caenorhabditis elegans

<p>Abstract</p> <p>Background</p> <p>While many molecules involved in axon guidance have been identified, the cellular and molecular mechanisms by which these molecules regulate growth cone morphology during axon outgrowth remain to be elucidated. The actin cytoskeleton of the growth cone underlies the formation of lamellipodia and filopodia that control growth cone outgrowth and guidance. The role of the Arp2/3 complex in growth cone filopodia formation has been controversial, and other mechanisms of growth cone filopodia formation remain to be described.</p> <p>Results</p> <p>Here we show that mutations in genes encoding the Arp2/3 complex (<it>arx </it>genes) caused defects in axon guidance. Analysis of developing growth cones <it>in vivo </it>showed that <it>arx </it>mutants displayed defects in filopodia and reduced growth cone size. Time-lapse analysis of growth cones in living animals indicated that <it>arx </it>mutants affected the rate of growth cone filopodia formation but not filopodia stability or length. Two other actin modulatory proteins, UNC-115/abLIM and UNC-34/Enabled, that had been shown previously to affect axon guidance had overlapping roles with Arp2/3 in axon guidance and also affected the rate of filopodia initiation but not stability or length.</p> <p>Conclusion</p> <p>Our results indicate that the Arp2/3 complex is required cell-autonomously for axon guidance and growth cone filopodia initiation. Furthermore, they show that two other actin-binding proteins, UNC-115/abLIM and UNC-34/Enabled, also control growth cone filopodia formation, possibly in parallel to Arp2/3. These studies indicate that, <it>in vivo</it>, multiple actin modulatory pathways including the Arp2/3 complex contribute to growth cone filopodia formation during growth cone outgrowth.</p

Star-Formation in the Ultraluminous Infrared Galaxy F00183-7111

We report the detection of molecular CO(1-0) gas in F00183-7111, one of the most extreme Ultra-Luminous Infrared Galaxies known, with the Australia Telescope Compact Array. We measure a redshift of 0.3292 for F00183-7111 from the CO(1-0) line and estimate the mass of the molecular gas in 00183 to be 1 $\times$ 10$^{10}$ M$_{\odot}$. We find that F00183-7111 is predominately powered by the AGN and only $\sim$14 per cent of the total luminosity is contributed by star-formation (SFR $\sim$220 M$_{\odot}$ yr$^{-1}$). We also present an optical image of F00183-7111, which shows an extension to the East. We searched for star-formation in this extension using radio continuum observations but do not detect any. This suggests that the star-formation is likely to be predominately nuclear. These observations provide additional support for a model in which the radio emission from ULIRGs is powered by an intense burst of star-formation and by a radio-loud AGN embedded in its nucleus, both triggered by a merger of gas-rich galaxies.Comment: 5 pages, 2 figures, Accepted for publication in MNRAS Letters Accepted 2014 January 19. Received 2013 December 30; in original form 2013 November 2

Iris: an Extensible Application for Building and Analyzing Spectral Energy Distributions

Iris is an extensible application that provides astronomers with a user-friendly interface capable of ingesting broad-band data from many different sources in order to build, explore, and model spectral energy distributions (SEDs). Iris takes advantage of the standards defined by the International Virtual Observatory Alliance, but hides the technicalities of such standards by implementing different layers of abstraction on top of them. Such intermediate layers provide hooks that users and developers can exploit in order to extend the capabilities provided by Iris. For instance, custom Python models can be combined in arbitrary ways with the Iris built-in models or with other custom functions. As such, Iris offers a platform for the development and integration of SED data, services, and applications, either from the user's system or from the web. In this paper we describe the built-in features provided by Iris for building and analyzing SEDs. We also explore in some detail the Iris framework and software development kit, showing how astronomers and software developers can plug their code into an integrated SED analysis environment.Comment: 18 pages, 8 figures, accepted for publication in Astronomy & Computin

The European Federation of Organisations for Medical Physics policy statement no 14 : the role of the medical physicist in the management of safety within the magnetic resonance imaging environment : EFOMP recommendations

This European Federation of Organisations for Medical Physics (EFOMP) Policy Statement outlines the way in which a Safety Management System can be developed for MRI units. The Policy Statement can help eliminate or at least minimize accidents or incidents in the magnetic resonance environment and is recommended as a step towards harmonisation of safety of workers, patients, and the general public regarding the use of magnetic resonance imaging systems in diagnostic and interventional procedures.peer-reviewe

The effects of physical contact type on the internal and external demands during a rugby league match simulation protocol.

This is an Accepted Manuscript of an article published by Taylor & Francis in Journal of Sports Sciences on 09/02/2016, available online: DOI: 10.1080/02640414.2016.1140907This study investigated how the type of contact influences physiological, perceptual and locomotive demands during a simulated rugby league match. Eleven male university rugby league players performed two trials of the rugby league movement simulation protocol for forwards (RLMSP-i) with a traditional soft tackle bag (BAG) and a weighted tackle sled (SLED) to replicate contact demands. Locomotive rate, sprint speed, tackle intensity, heart rate, rating of perceived exertion and blood lactate concentration were analysed in four periods during the first and second bout of both trials. Countermovement jump (CMJ) was measured before and immediately after each trial. More time was spent in heart rate zone between 90 – 100% HRpeak during the first (effect size ± 95% confidence interval: 0.44 ± 0.49) and second bout (0.44 ± 0.43), and larger (0.6 ± 0.69) decrements in CMJ performance were observed during SLED (5.9, s = 4.9%) compared to BAG (2.6, s = 5.4%). Sprint into contact speed was faster during BAG compared to SLED in the first (1.10 ± 0.92) and second bout (0.90 ± 0.90), which impaired high intensity running ability but did not increase physiological strain. Changing the type of contact during the match simulation subtly altered both the internal and external load on participants. These findings indicate that tackle training apparatus should be considered regarding the outcome of a training session

Landscape of overlapping gene expression in the equine placenta

Increasing evidence suggests that overlapping genes are much more common in eukaryotic genomes than previously thought. These different-strand overlapping genes are potential sense-antisense (SAS) pairs, which might have regulatory effects on each other. In the present study, we identified the SAS loci in the equine genome using previously generated stranded, paired-end RNA sequencing data from the equine chorioallantois. We identified a total of 1261 overlapping loci. The ratio of the number of overlapping regions to chromosomal length was numerically higher on chromosome 11 followed by chromosomes 13 and 12. These results show that overlapping transcription is distributed throughout the equine genome, but that distributions differ for each chromosome. Next, we evaluated the expression patterns of SAS pairs during the course of gestation. The sense and antisense genes showed an overall positive correlation between the sense and antisense pairs. We further provide a list of SAS pairs with both positive and negative correlation in their expression patterns throughout gestation. This study characterizes the landscape of sense and antisense gene expression in the placenta for the first time and provides a resource that will enable researchers to elucidate the mechanisms of sense/antisense regulation during pregnancy