Sporstoff-forsøk i Ranfjorden 2012-2013

Rapporten omhandlar resultat av sporstoff-forsøk gjennomført av NIVA i Ranfjorden i 2012 og 2013, i samband med vurderingar for spreiinga av fin- og grovfraksjon avgangen frå malmoppredninga ved Rana Gruber. Eksisterande avgangsløysing med utslepp på 45 m (finfraksjon) og 30 m (grovfraksjon) har medført nedslamming av botnen i indre deler av fjorden, og spreiing av finstoffet i sjøen rundt. Sporstoff-forsøka vart gjennomført for å verifisere denne spreiinga. Finstoffet når i hovudsak ikkje grunnare enn 8 m djup for finfraksjon, og 25 m djup for grov. Det var sporadisk utslag for sporstoff nær overflata som kan vere assosiert med den finaste delen av finfraksjonen.Rana Gruber A

FerryBox-assisted monitoring of mixed layer pH in the Norwegian Coastal Current

The evaluation of marine carbonate system variability and the impacts of ocean acidification (OA) on coastal marine ecosystems increasingly rely on monitoring platforms capable of delivering near real-time in situ carbonate system observations. These observations are also used for developing models and scenarios of OA, including potential impacts on marine ecosystem structure and function. An embedded flow-through spectrophotometric pH detection system has been developed alongside an underway seawater sampling system – termed a FerryBox – operating on ships of opportunity (SOOP), and can deliver a continuous data stream of mixed layer seawater pH with an in situ uncertainty of < 0.003. We report metrological approaches behind the pH detection procedure and the evaluation of dye addition perturbation with analytical precision as low as 0.0005. In addition, we present field-based observations from a deployment of the pH detection system along the Norwegian Coastal Current in winter, spring, and summer periods of 2015. Spring and summertime pH was generally ~ 0.1 higher, and up to ~ 0.255 higher, in comparison to winter pH observations. Here we show the necessity for a regular, high density monitoring approach, and the suitability of this pH detection technique for unmanned observational platforms

Ciprofloxacin removal from non-clinical environment: A critical review of current methods and future trend prospects

Antibiotics in the environment represent a significant threat to global public health. Ciprofloxacin (CIP) is one of the second generation groups of synthetic fluoroquinolones and the most widely used antibiotics worldwide. The current work aimed to review and analyze the current methods used for eliminating CIP and identify the ap�proaches for more advanced technologies that could provide more removal efficiency for CIP removal from the non-clinical environment. The VOSviewer software tool was used to build and visualize bibliometric networks by creating a map based on bibliographic data for keywords and most countries published on the CIP removal from the Scopus database. The present review analyses the sustainable methods for removing CIP from the non-clinical environment and highlights the most efficient techniques used to remove CIP. The adsorption process of CIP is highly efficient, with a removal percentage of 95%. The microbial electrolysis ultraviolet cell (MEUC) procedure removed 100% of CIP. The degradation of CIP by UV/H2O2/O3 and its sub-processes increased the degradation of CIP from 41.2% to 98.5%. The photocatalytic degradation exhibit 92.81% removal of CIP from wastewater samples. The three-dimensional (3D) porous graphene has excellent adsorption properties to eliminate CIP by 93% in water purification. Each method has advantages and disadvantages based on price, time and presence of toxic by-products. This review is expected to serve as a base for recent research and assist researchers in developing alternative CIP treatment approaches with more efficient removal methods

Reduction of bacteria in storage system of sewage effluents

The present work is aimed to investigate the linear regression model of total coliform (TC), faecal coli-form (FC) and enterococci (ENT) responses in the storage system of sewage effluents at different temperatures (room temperature 25 ± 2 °C, 55 and 65 °C). Five litres (v/v) of sewage effluent samples was stored at room temperature (25 ± 2 °C) for 1, 2, 3 and 4 weeks. In order to investigate the response of bacteria to the storage system at thermal conditions, the sewage samples were stored at the tempera-tures of 55 and 65 °C in a water bath shaker for 10, 20, 30, 40, 50, 60, 70, 80, 90, 100, 110 and 120 min, respectively. The results indicated that the storage system at room tem-perature significantly (p < 0.01) effected the reduction of TC (33%), FC (36.6%) and ENT (47.8%). Moreover, sew-age effluent met WHO guidelines after two weeks of stor-age period at room temperature. The storage system at 55 and 65 °C had more significant influence (p < 0.01) on TC, FC and ENT. The reductions for TC, FC and ENT were 49.6 versus 64, 47.7 versus 83.2% and 47.4 versus 57.3%, respectively. ENT (Gram-positive bacteria) exhibited more resistance to the storage system at 65 °C than TC and FC (both are Gram-negative bacteria). This might be due to the differences in the cell wall structure. It can be concluded that the storage system of sewage effluents has a significant potential for the reduction of indicator bacteria

Methotrexate Treatment of Newly Diagnosed RA Patients Is Associated With DNA Methylation Differences at Genes Relevant for Disease Pathogenesis and Pharmacological Action

Background: Methotrexate (MTX) is the fi rst line treatment of rheumatoid arthritis (RA), and methylation changes in bulk T cells have been reported after treatment with MTX. We have investigated cell-type speci fi c DNA methylation changes across the genome in naïve and memory CD4 + T cells before and after MTX treatment of RA patients. DNA methylation pro fi les of newly diagnosed RA patients (N=9) were assessed by reduced representation bisul fi te sequencing. Results: We found that MTX treatment signi fi cantly in fl uenced DNA methylation levels at multiple CpG sites in both cell populations. Interestingly, we identi fi ed differentially methylated sites annotated to two genes; TRIM15 and SORC2, previously reported to predict treatment outcome in RA patients when measured in bulk T cells. Furthermore, several of the genes, including STAT3, annotated to the signi fi cant CpG sites are relevant for RA susceptibility or the action of MTX. Conclusion: We detected CpG sites that were associated with MTX treatment in CD4 + naïve and memory T cells isolated from RA patients. Several of these sites overlap genetic regions previously associated with RA risk and MTX treatment outcome

A global database of dissolved organic matter (DOM) concentration measurements in coastal waters (CoastDOM v1)

Measurements of dissolved organic carbon (DOC), nitrogen (DON), and phosphorus (DOP) con-centrations are used to characterize the dissolved organic matter (DOM) pool and are important components ofbiogeochemical cycling in the coastal ocean. Here, we present the first edition of a global database (CoastDOMv1; available at https://doi.org/10.1594/PANGAEA.964012, L\uf8nborg et al., 2023) compiling previously pub-lished and unpublished measurements of DOC, DON, and DOP in coastal waters. These data are complementedby hydrographic data such as temperature and salinity and, to the extent possible, other biogeochemical variables(e.g. chlorophyll a, inorganic nutrients) and the inorganic carbon system (e.g. dissolved inorganic carbon andtotal alkalinity). Overall, CoastDOM v1 includes observations of concentrations from all continents. However,most data were collected in the Northern Hemisphere, with a clear gap in DOM measurements from the SouthernHemisphere. The data included were collected from 1978 to 2022 and consist of 62 338 data points for DOC,20 356 for DON, and 13 533 for DOP. The number of measurements decreases progressively in the sequenceDOC &gt; DON &gt; DOP, reflecting both differences in the maturity of the analytical methods and the greater focuson carbon cycling by the aquatic science community. The global database shows that the average DOC concen-tration in coastal waters (average \ub1 standard deviation (SD): 182 \ub1 314 μmol C L−1; median: 103 μmol C L−1) is13-fold higher than the average coastal DON concentration (13.6 \ub1 30.4 μmol N L−1; median: 8.0 μmol N L−1),which is itself 39-fold higher than the average coastal DOP concentration (0.34 \ub1 1.11 μmol P L−1; median:0.18 μmol P L−1). This dataset will be useful for identifying global spatial and temporal patterns in DOM and willhelp facilitate the reuse of DOC, DON, and DOP data in studies aimed at better characterizing local biogeochem-ical processes; closing nutrient budgets; estimating carbon, nitrogen, and phosphorous pools; and establishing abaseline for modelling future changes in coastal waters