Understanding temporal rhythms and travel behaviour at destinations: Potential ways to achieve more sustainable travel

This paper analyses the roles played by time in destination-based travel behaviour. It contrasts clock time's linear view of time with fragmented time, instantaneous time, fluid time and flow, time out and the multiple temporalities of tourism experiences. It explores temporal issues in a destination travel context, using qualitative techniques. Data were captured using diary photography, diary-interview method with tourists at a rural destination; their spatial and temporal patterns were captured using a purpose built smartphone app. The analysis revealed three temporal themes influencing travel behaviour: time fluidity; daily and place-related rhythms; and control of time. Three key messages emerge for future sustainable tourist destination-based travel systems. Given the strong desire for temporal fluidity, transport systems should evolve beyond clock-time regimes. Second, temporal forces favour personal modes of transport (car, walk, cycle), especially in rural areas where public transport cannot offer flexibility. Third, the car is personalised and perceived to optimise travel fluidity and speed, but is currently unsustainable. Imaginative initiatives, using new mobile media technology can offer new positive and proactive car travel, utilising spare public and private vehicle capacity. Research is needed to implement mechanisms for individualised space-time scheduling and collective vehicle use strategies. © 2013 Copyright Taylor and Francis Group, LLC

Sixth Sense Transport : Challenges in Supporting Flexible Time Travel

In this paper, we consider the challenges associated with providing a mobile computing system that helps users enjoy a more flexible relationship between time and travel. Current travel plans, especially in Western cultures, are dominated by a strict notion of time. The need to conform to schedules leads to increased pressures for travellers and inefficiencies when these schedules cannot be met. We are interested in exploring the extent to which mobile computing can be used to help travellers relax these schedules and adopt a more opportunistic approach to travel – potentially helping to reduce the environmental, financial and societal costs of modern travel

Clinical features, anaesthetic management and perioperative complications seen in three horses with pheochromocytoma

Three horses presenting with colic signs to the Equine Referral Hospital at The Royal Veterinary College underwent general anaesthesia between September 2013 and November 2017 for emergency exploratory laparotomy. No obvious cause for the colic signs was identified in two horses, while a haemoperitoneum was identified in the third. All horses were euthanased within 12 hours of surgery due to deteriorating haemodynamic instability and/or intractable pain. Postmortem examination revealed an adrenal mass in each case, confirmed to be a pheochromocytoma on histopathology. In retrospect, each horse had some hallmark characteristics consistent with a functional pheochromocytoma, including hyperglycaemia and hyperlactataemia. Extremely high packed cell volume (PCV) (>65 per cent) was also identified in two horses, with a high-normal PCV found in the haemoperitoneum case. Perioperative haemodynamic instability was predominantly characterised by episodes of intermittent hypertension and tachycardia

Homeless population

The aim was to derive and analyze a model for numbers of homeless and non-homeless people in a borough, in particular to see how these figures might be affected by different policies regarding housing various categories of people. Most attention was focused on steady populations although the stability of these and possible timescales of dynamic problems were also discussed. The main outcome of this brief study is the identification of the key role played by the constant k_1 - the constant which fixes the speed at which the homeless are rehoused in permanent council property. Reducing this constant, i.e. making the system "fairer" with less priority to accommodating homeless families, appears to have little effect on the sizes of other categories on the waiting list but there is a marked increase in the number of households in temporary accommodation. The model, indicated by the size of its longest time-scale, should be modified to allow for births etc. It could be varied by allowing people to remove themselves from the register or by allowing the rates at which registered and unregistered people become homeless to differ, but these modifications are unlikely to substantially change the main result. The inclusion of movement from the homeless to the general population would have the effect of limiting the numbers in temporary accommodation. However, it is thought this effect is very small so a great reduction in k_1 would be needed for this flow to become significant

Syntaxin 5 Is Required for Copper Homeostasis in Drosophila and Mammals

Copper is essential for aerobic life, but many aspects of its cellular uptake and distribution remain to be fully elucidated. A genome-wide screen for copper homeostasis genes in Drosophila melanogaster identified the SNARE gene Syntaxin 5 (Syx5) as playing an important role in copper regulation; flies heterozygous for a null mutation in Syx5 display increased tolerance to high dietary copper. The phenotype is shown here to be due to a decrease in copper accumulation, a mechanism also observed in both Drosophila and human cell lines. Studies in adult Drosophila tissue suggest that very low levels of Syx5 result in neuronal defects and lethality, and increased levels also generate neuronal defects. In contrast, mild suppression generates a phenotype typical of copper-deficiency in viable, fertile flies and is exacerbated by co-suppression of the copper uptake gene Ctr1A. Reduced copper uptake appears to be due to reduced levels at the plasma membrane of the copper uptake transporter, Ctr1. Thus Syx5 plays an essential role in copper homeostasis and is a candidate gene for copper-related disease in humans

Control of Alzheimer's Amyloid Beta Toxicity by the High Molecular Weight Immunophilin FKBP52 and Copper Homeostasis in Drosophila

FK506 binding proteins (FKBPs), also called immunophilins, are prolyl-isomerases (PPIases) that participate in a wide variety of cellular functions including hormone signaling and protein folding. Recent studies indicate that proteins that contain PPIase activity can also alter the processing of Alzheimer's Amyloid Precursor Protein (APP). Originally identified in hematopoietic cells, FKBP52 is much more abundantly expressed in neurons, including the hippocampus, frontal cortex, and basal ganglia. Given the fact that the high molecular weight immunophilin FKBP52 is highly expressed in CNS regions susceptible to Alzheimer's, we investigated its role in Aβ toxicity. Towards this goal, we generated Aβ transgenic Drosophila that harbor gain of function or loss of function mutations of FKBP52. FKBP52 overexpression reduced the toxicity of Aβ and increased lifespan in Aβ flies, whereas loss of function of FKBP52 exacerbated these Aβ phenotypes. Interestingly, the Aβ pathology was enhanced by mutations in the copper transporters Atox1, which interacts with FKBP52, and Ctr1A and was suppressed in FKBP52 mutant flies raised on a copper chelator diet. Using mammalian cultures, we show that FKBP52 (−/−) cells have increased intracellular copper and higher levels of Aβ. This effect is reversed by reconstitution of FKBP52. Finally, we also found that FKBP52 formed stable complexes with APP through its FK506 interacting domain. Taken together, these studies identify a novel role for FKBP52 in modulating toxicity of Aβ peptides

Detection of Genetically Altered Copper Levels in Drosophila Tissues by Synchrotron X-Ray Fluorescence Microscopy

Tissue-specific manipulation of known copper transport genes in Drosophila tissues results in phenotypes that are presumably due to an alteration in copper levels in the targeted cells. However direct confirmation of this has to date been technically challenging. Measures of cellular copper content such as expression levels of copper-responsive genes or cuproenzyme activity levels, while useful, are indirect. First-generation copper-sensitive fluorophores show promise but currently lack the sensitivity required to detect subtle changes in copper levels. Moreover such techniques do not provide information regarding other relevant biometals such as zinc or iron. Traditional techniques for measuring elemental composition such as inductively coupled plasma mass spectroscopy are not sensitive enough for use with the small tissue amounts available in Drosophila research. Here we present synchrotron x-ray fluorescence microscopy analysis of two different Drosophila tissues, the larval wing imaginal disc, and sectioned adult fly heads and show that this technique can be used to detect changes in tissue copper levels caused by targeted manipulation of known copper homeostasis genes

Pancreas transplantation with grafts obtained from donation after cardiac death or donation after brain death results in comparable outcomes

IntroductionPancreas organ shortages and long recipient waitlist times are critical components that limit recipients from receiving a pancreas transplant. Over the last decade, our center has been using donation after cardiac death (DCD) donors as an adjunct to donation after brain death (DBD) donors to expand the organ pool. The aim of this study was to compare recipient and graft survival between DCD and DBD recipients.MethodsA retrospective single center propensity matched analysis (2011–2020) of 32 DCD vs 96 DBD pancreas transplants was performed.Results8-year recipient survival was similar between DCD and DBD groups (87.4% vs 92.7%, p=0.35) as was simultaneous kidney and pancreas transplant (SPK) 8-year kidney (88.9 vs 96.9%, p=0.219) and pancreas graft survival (77.4% vs 86.7%, p=0.344). There was no difference in vascular thrombosis rate between DCD and DBD pancreas grafts (3.1% vs 7.3%, p=0.73). DCD kidneys had a higher rate of DGF vs DBD kidneys (28.1% vs 6.3%, p=0.004), without any significant difference in long term kidney failure (12.5% vs 8.3%, p=0.5).DiscussionRecipients of DCD grafts demonstrate equivalent long-term patient and graft survival compared to DBD recipients for pancreas transplantation. Increased utilization of well selected DCD donors is a safe strategy to increase the donor pool

Ambient Temperature Influences Australian Native Stingless Bee (Trigona carbonaria) Preference for Warm Nectar

The interaction between flowers and insect pollinators is an important aspect of the reproductive mechanisms of many plant species. Several laboratory and field studies indicate that raising flower temperature above ambient can be an advantage in attracting pollinators. Here we demonstrate that this preference for warmer flowers is, in fact, context-dependent. Using an Australian native bee as a model, we demonstrate for the first time a significant shift in behaviour when the ambient temperature reaches 34°C, at which point bees prefer ambient temperature nectar over warmer nectar. We then use thermal imaging techniques to show warmer nectar maintains the flight temperature of bees during the period of rest on flowers at lower ambient temperatures but the behavioural switch is associated with the body temperature rising above that maintained during flight. These findings suggest that flower-pollinator interactions are dependent upon ambient temperature and may therefore alter in different thermal environments

Método para quantificação do consumo energético no ciclo de vida de equipamentos hidrossanitários

A escolha entre diferentes materiais, produtos ou sistemas empregados na construção civil pode levar em consideração diferentes critérios, sejam estes econômicos, sociais, culturais e ambientais. No caso de equipamentos hidrossanitários, a avaliação econômica e do consumo de recursos, tais como a água, são os critérios mais comumente empregados. O presente artigo objetiva a proposição de um método para quantificação do consumo energético de equipamentos hidrossanitários com base no conceito de Análise do Ciclo de Vida. Essa quantificação permite a comparação entre o desempenho de diferentes equipamentos hidrossanitários com base no dispêndio energético total. No presente artigo o método proposto é aplicado em um estudo de caso analisando dois modelos de torneira (convencional e com fechamento hidromecânico). O consumo energético total no ciclo de vida das torneiras convencional e economizadora de água utilizadas no estudo foi de respectivamente, 151,66 MJ e 127,58 MJ. A etapa de uso das torneiras apresentou peso preponderante nos resultados, com participação de 64,6% para a torneira convencional e 56,5% para a torneira com fechamento hidromecânico. O método proposto se mostrou adequado para quantificação do consumo energético no ciclo de vida e posterior escolha entre diferentes equipamentos hidrossanitários que exerçam a mesma função