Predictors of major complications and the association with oncological outcomes after radical cystectomy for bladder cancer:A nationwide registry study

Introduction:Radical cystectomy improves survival of patients with muscle invasive and high-risk non-muscle invasive bladder cancer, but is a challenging surgical procedure as patients may experience major complications after surgery.Objectives:To assess the incidence of Clavien-Dindo ≥3 complications in patients who underwent radical cystectomy and to assess the association of these complications with pre-operative and peroperative parameters. The secondary aim was to study the association of complications with long-term oncological outcome.Methods:A nationwide registry was set up in 19 Dutch hospitals that studied patients with muscle invasive bladder cancer and high-risk non-muscle invasive bladder cancer treated by radical cystectomy. Major complications were classified as complications that were related to uretero-ileal anastomosis, intra-abdominal (e.g. urinoma, bowel leakage) infectious and cardiovascular complications. Multivariable logistic regression analyses were performed to assess the correlation between these groups and perioperative, clinical and pathological factors. Kaplan-Meier survival curves were constructed to analyze the correlation between complications and overall survival.Results:The study population consisted of 1,464 patients, of whom 420 (29%) developed severe complications. The most common complications were intra-abdominal (n=328, 60%) and uretero-ileal anastomosis related (n=92, 17%). Male gender (odds ratio 1.6, p=0.007), American Society of Anaesthesiologists score ≥3 (odds ratio 1.6, p=0.003), Charlson Comorbidity Index score ≥5 (odds ratio 2.1, p=0.002) and blood loss >700ml (odds ratio 1.4, p=0.044) were associated with severe complications. In addition, open radical cystectomy was associated with multiple complications (odds ratio 2.6, p=0.001). Furthermore, the overall survival of patients with major complications was worse than those who had no major complications. The median overall survival was 3.8 years versus 6.2 years for patients with and without severe complications (p<0.001).Conclusions:In a real-world setting, 29% of patients undergoing radical cystectomy developed severe complications. The risk of severe complications was higher in men, patients with impaired pre-operative condition, and in those who underwent open surgery. Severe complications had a negative impact on overall survival

Soil and Rhizosphere Associated Fungi in Gray Mangroves (Avicennia marina) from the Red Sea — A Metagenomic Approach

Covering a quarter of the world’s tropical coastlines and being one of the most threatened ecosystems, mangroves are among the major sources of terrestrial organic matter to oceans and harbor a wide microbial diversity. In order to protect, restore, and better understand these ecosystems, researchers have extensively studied their microbiology, yet few surveys have focused on their fungal communities. Our lack of knowledge is even more pronounced for specific fungal populations, such as the ones associated with the rhizosphere. Likewise, the Red Sea gray mangroves (Avicennia marina) remain poorly characterized, and understanding of their fungal communities still relies on cultivation-dependent methods. In this study, we analyzed metagenomic datasets from gray mangrove rhizosphere and bulk soil samples collected in the Red Sea coast, to obtain a snapshot of their fungal communities. Our data indicated that Ascomycota was the dominant phylum (76%–85%), while Basidiomycota was less abundant (14%–24%), yet present in higher numbers than usually reported for such environments. Fungal communities were more stable within the rhizosphere than within the bulk soil, both at class and genus level. This finding is consistent with the intrinsic patchiness in soil sediments and with the selection of specific microbial communities by plant roots. Our study indicates the presence of several species on this mycobiome that were not previously reported as mangrove-associated. In particular, we detected representatives of several commercially-used fungi, e.g., producers of secreted cellulases and anaerobic producers of cellulosomes. These results represent additional insights into the fungal community of the gray mangroves of the Red Sea, and show that they are significantly richer than previously reported

Inclusive quarkonium production in pp collisions at √s = 5.02 TeV

This article reports on the inclusive production cross section of several quarkonium states, $\textrm{J}/\psi$, $\psi \mathrm{(2S)}$, $\Upsilon \mathrm (1S)$, $\Upsilon \mathrm{(2S)}$, and $\Upsilon \mathrm{(3S)}$, measured with the ALICE detector at the LHC, in pp collisions at $\sqrt{s} = 5.02$ TeV. The analysis is performed in the dimuon decay channel at forward rapidity ($2.5< y < 4$). The integrated cross sections and transverse-momentum ($p_{\textrm{T}}$) and rapidity ($y$) differential cross sections for $\textrm{J}/\psi$, $\psi \mathrm{(2S)}$, $\Upsilon \mathrm (1S)$, and the $\psi \mathrm{(2S)}$-to-$\textrm{J}/\psi$ cross section ratios are presented. The integrated cross sections, assuming unpolarized quarkonia, are: $\sigma _{\textrm{J}/\psi }$ ($p_{\textrm{T}} <20$ GeV/c) = 5.88 ± 0.03 ± 0.34$~\mu$b, $\sigma _{\psi \mathrm{(2S)}}$ ($p_{\textrm{T}} <12$ GeV/c) = 0.87 ± 0.06 ± 0.10$~\mu$b, $\sigma _{\Upsilon \mathrm (1S)}$ ($p_{\textrm{T}} <15$ GeV/c) = 45.5 ± 3.9 ± 3.5 nb, $\sigma _{\Upsilon \mathrm{(2S)}}$ ($p_{\textrm{T}} <15$ GeV/c) = 22.4 ± 3.2 ± 2.7 nb, and $\sigma _{\Upsilon \mathrm{(3S)}}$ ($p_{\textrm{T}} <15$ GeV/c) = 4.9 ± 2.2 ± 1.0 nb, where the first (second) uncertainty is the statistical (systematic) one. For the first time, the cross sections of the three $\Upsilon$ states, as well as the $\psi \mathrm{(2S)}$ one as a function of $p_{\textrm{T}}$ and $y$, are measured at $\sqrt{s} = 5.02$ TeV at forward rapidity. These measurements also significantly extend the $\textrm{J}/\psi$ $p_{\textrm{T}}$ reach and supersede previously published results. A comparison with ALICE measurements in pp collisions at $\sqrt{s} = 2.76$, 7, 8, and 13 TeV is presented and the energy dependence of quarkonium production cross sections is discussed. Finally, the results are compared with the predictions from several production models.publishedVersio

Inclusive J/ψ production at midrapidity in pp collisions at √s=13 TeV

We report on the inclusive $\text {J}/\psi$ production cross section measured at the CERN Large Hadron Collider in proton–proton collisions at a center-of-mass energy $\sqrt{s}~=~13$ TeV. The $\text {J}/\psi$ mesons are reconstructed in the $\text {e}^{+}\text {e}^{-}$ decay channel and the measurements are performed at midrapidity ($|y|<0.9$) in the transverse-momentum interval $0<p_{\mathrm{T}} <40$ GeV/$c$, using a minimum-bias data sample corresponding to an integrated luminosity $L_{\text {int}} = 32.2~\text {nb}^{-1}$ and an Electromagnetic Calorimeter triggered data sample with $L_{\text {int}} = 8.3~\mathrm {pb}^{-1}$. The $p_{\mathrm{T}}$-integrated $\text {J}/\psi$ production cross section at midrapidity, computed using the minimum-bias data sample, is $\text {d}\sigma /\text {d}y|_{y=0} = 8.97\pm 0.24~(\text {stat})\pm 0.48~(\text {syst})\pm 0.15~(\text {lumi})~\mu \text {b}$. An approximate logarithmic dependence with the collision energy is suggested by these results and available world data, in agreement with model predictions. The integrated and $p_{\mathrm{T}}$-differential measurements are compared with measurements in pp collisions at lower energies and with several recent phenomenological calculations based on the non-relativistic QCD and Color Evaporation models.publishedVersio

First measurements of N-subjettiness in central Pb-Pb collisions at √sNN = 2.76 TeV

The ALICE Collaboration reports the first fully-corrected measurements of the N-subjettiness observable for track-based jets in heavy-ion collisions. This study is performed using data recorded in pp and Pb-Pb collisions at centre-of-mass energies of s√ = 7 TeV and sNN−−−√ = 2.76 TeV, respectively. In particular the ratio of 2-subjettiness to 1-subjettiness, τ2/τ1, which is sensitive to the rate of two-pronged jet substructure, is presented. Energy loss of jets traversing the strongly interacting medium in heavy-ion collisions is expected to change the rate of two-pronged substructure relative to vacuum. The results are presented for jets with a resolution parameter of R = 0.4 and charged jet transverse momentum of 40 ≤ pT,jet ≤ 60 GeV/c, which constitute a larger jet resolution and lower jet transverse momentum interval than previous measurements in heavy-ion collisions. This has been achieved by utilising a semi-inclusive hadron-jet coincidence technique to suppress the larger jet combinatorial background in this kinematic region. No significant modification of the τ2/τ1 observable for track-based jets in Pb-Pb collisions is observed relative to vacuum PYTHIA6 and PYTHIA8 references at the same collision energy. The measurements of τ2/τ1, together with the splitting aperture angle ∆R, are also performed in pp collisions at s√ = 7 TeV for inclusive jets. These results are compared with PYTHIA calculations at s√ = 7 TeV, in order to validate the model as a vacuum reference for the Pb-Pb centre-of-mass energy. The PYTHIA references for τ2/τ1 are shifted to larger values compared to the measurement in pp collisions. This hints at a reduction in the rate of two-pronged jets in Pb-Pb collisions compared to pp collisions.publishedVersio

Anisotropic flow of identified hadrons in Xe-Xe collisions at √sNN = 5.44 TeV

Measurements of elliptic (v2) and triangular (v3) flow coefficients of π±, K±, p+$\overline{\mathrm{p}}$, ${\mathrm{K}}_{\mathrm{S}}^0$, and Λ+$\overline{\Lambda}$ obtained with the scalar product method in Xe-Xe collisions at $\sqrt{s_{\mathrm{NN}}}$ = 5.44 TeV are presented. The results are obtained in the rapidity range |y| < 0.5 and reported as a function of transverse momentum, pT, for several collision centrality classes. The flow coefficients exhibit a particle mass dependence for pT < 3 GeV/c, while a grouping according to particle type (i.e., meson and baryon) is found at intermediate transverse momenta (3 < pT < 8 GeV/c). The magnitude of the baryon v2 is larger than that of mesons up to pT = 6 GeV/c. The centrality dependence of the shape evolution of the pT-differential v2 is studied for the various hadron species. The v2 coefficients of π±, K±, and p+$\overline{\mathrm{p}}$ are reproduced by MUSIC hydrodynamic calculations coupled to a hadronic cascade model (UrQMD) for pT < 1 GeV/c. A comparison with vn measurements in the corresponding centrality intervals in Pb-Pb collisions at $\sqrt{s_{\mathrm{NN}}}$ = 5.02 TeV yields an enhanced v2 in central collisions and diminished value in semicentral collisions.publishedVersio

Experimental Evidence for an Attractive p-φ Interaction

This Letter presents the first experimental evidence of the attractive strong interaction between a proton and a ϕ meson. The result is obtained from two-particle correlations of combined p-ϕ⊕ p¯-ϕ pairs measured in high-multiplicity pp collisions at s√=13  TeV by the ALICE Collaboration. The spin-averaged scattering length and effective range of the p-ϕ interaction are extracted from the fully corrected correlation function employing the Lednický-Lyuboshits approach. In particular, the imaginary part of the scattering length vanishes within uncertainties, indicating that inelastic processes do not play a prominent role for the p-ϕ interaction. These data demonstrate that the interaction is dominated by elastic p-ϕ scattering. Furthermore, an analysis employing phenomenological Gaussian- and Yukawa-type potentials is conducted. Under the assumption of the latter, the N-ϕ coupling constant is found to be gN-ϕ=0.14±0.03(stat)±0.02(syst). This work provides valuable experimental input to accomplish a self-consistent description of the N-ϕ interaction, which is particularly relevant for the more fundamental studies on partial restoration of chiral symmetry in nuclear medium.publishedVersio

KS0 - and (anti-) Λ -hadron correlations in pp collisions at √s=13 TeV

Two-particle Azimuthal correlations are measured with the ALICE apparatus in pp collisions at s√=13s=13 TeV to explore strangeness- and multiplicity-related effects in the fragmentation of jets and the transition regime between bulk and hard production, probed with the condition that a strange meson (KS0KS0) or baryon (ΛΛ) with transverse momentum pT>3pT>3 GeV/cc is produced. Azimuthal correlations between kaons or ΛΛ hyperons with other hadrons are presented at midrapidity for a broad range of the trigger (3<ptriggT<203<pTtrigg<20 GeV/cc) and associated particle pTpT (1 GeV/cc <passocT<ptriggT<pTassoc<pTtrigg), for minimum-bias events and as a function of the event multiplicity. The near- and away-side peak yields are compared for the case of either KS0KS0 or ΛΛ(Λ¯¯¯¯Λ¯) being the trigger particle with that of inclusive hadrons (a sample dominated by pions). In addition, the measurements are compared with predictions from PYTHIA 8 and EPOS LHC event generators.publishedVersio

UPAYA MENINGKATKAN MOTIVASI BELAJAR SISWA MELALUI MEDIA GAME EDUCATION DALAM PEMBELAJARAN IPS DI MTs HASBUR RACHMAN ALIF DESA LENGKONG WETAN KABUPATEN MAJALENGKA

ASEP SAMSUL MA'ARIF : Berdasarkan Observasiawal di kelas VII MTs Hasbur Rachman Alif terlihat proses pembelajaran IPS masih menggunakan media spidol dan papan tulis. Sehingga banyak siswa yang kurang memperhatikan guru pada saat proses pembelajaran., Hal ini berakibat pada kejenuhan dan kebosanan yang berdampak pada hasil belajar siswa yang kurang dari KKM yang diterapkan di MTs Hasbur Rachman Alif yakni 70. Tujuan penelitian ini untuk mengetahui bagaimana penerapan pembelajaran dengan menggunakan media Game Education pada mata pelajaran IPS Terpadu, aktivitas siswa dan kinerja guru dengan pembelajaran dengan menggunakan media Game Education, dan mengetahui motivasi belajar siswa sebelum dan setelah diterapkannya pembelajaran dengan menggunakan media Game education pada mata pelajaran IPS Terpadu di MTs Hasbur Rachman Alif. Pembelajaran dengan menggunakan media Game Education menekankan motivasi siswa untuk berpikir kritis dan menuangkan. Hal ini memudahkan siswa untuk mengakses materi IPS yang banyak dimanapun, serta mempemudah daya ingat siswa tentang apayatgtelah didapatkan selama proses pembelajaran. Penelitian ini menggunakan Penelitian Tindakan Kelas (pTK) yang dilaksanakan selama tiga siklus. Teknik Pengumpulan data menggunakan observasi, tes, dan dokumentasi. Subjek penelitian ini adalah siswa kelas VII MTs Hasbur Rachman Alif sebanyak 31 siswa yang terdiri dari 18 siswa laki-laki dan 13 siswa perempuan. Pembelajaran dengan menggunakan media Game Education dapat meningkatkan motivasi belajar siswa hal ini terlihat dari hasil tes yang telah dilakukan selama tiga siklus, dimana pada pratindakan (pretes) nilai sebesar 65,25 dan ketuntasan belajar 25 yo, siklus I nilai rata-rata sebesar 76,75 dan ketuntasan_ belajar siswa sebesar 65,5%o siklus II nilai rata-rata siswa 85,75 dan nilai ketuntasan belajar sebesar 90% siklus III dengan nilai rata-rata 92,25 dan ketuntasan belajar mengalami peningkatanya itu 97,5yo. Dengan demikian dapat disimpulkan bahwa pembelajaran dengan menggunakan media Game Education merupakan salah satu upaya untuk meningkatkan hasil belajar siswa pada mata pelajaran IPS Terpadu di MTs Hasbur Rachman Alif. Kata Kunci: Game Education, MotivasiBelajar, PTK

Dehydroepiandrosterone sulfate mediates activation of transcription factors CREB and ATF-1 via a Gα11-coupled receptor in the spermatogenic cell line GC-2

AbstractDehydroepiandrosterone sulfate (DHEAS) is a circulating steroid produced in the adrenal cortex, brain, and gonads. Whereas a series of investigations attest to neuroprotective effects of the steroid in the brain, surprisingly little is known about the physiological effects of DHEAS on cells of the reproductive system. Here we demonstrate that DHEAS acting on the spermatogenic cell line GC-2 induces a time- and concentration-dependent phosphorylation of c-Src and Erk1/2 and activates the transcription factors activating transforming factor-1 (ATF-1) and cyclic AMP-responsive element binding protein (CREB). These actions are consistent with the non-classical signaling pathway of testosterone and suggest that DHEAS is a pro-androgen that is converted into testosterone in order to exert its biological activity. The fact, however, that steroid sulfatase mRNA was not detected in the GC-2 cells and the clear demonstration of DHEAS-induced activation of Erk1/2, ATF-1 and CREB after silencing the androgen receptor by small interfering RNA (siRNA) clearly contradict this assumption and make it appear unlikely that DHEAS has to be converted in the cytosol into a different steroid in order to activate the kinases and transcription factors mentioned. Instead, it is likely that the DHEAS-induced signaling is mediated through the interaction of the steroid with a membrane-bound G-protein-coupled receptor, since silencing of Guanine nucleotide-binding protein subunit alpha-11 (Gnα11) leads to the abolition of the DHEAS-induced stimulation of Erk1/2, ATF-1, and CREB. The investigation presented here shows a hormone-like activity of DHEAS on a spermatogenic cell line. Since DHEAS is produced in male and female reproductive organs, these findings could help to define new roles for DHEAS in the physiology of reproduction