The IPIN 2019 Indoor Localisation Competition—Description and Results

IPIN 2019 Competition, sixth in a series of IPIN competitions, was held at the CNR Research Area of Pisa (IT), integrated into the program of the IPIN 2019 Conference. It included two on-site real-time Tracks and three off-site Tracks. The four Tracks presented in this paper were set in the same environment, made of two buildings close together for a total usable area of 1000 m 2 outdoors and and 6000 m 2 indoors over three floors, with a total path length exceeding 500 m. IPIN competitions, based on the EvAAL framework, have aimed at comparing the accuracy performance of personal positioning systems in fair and realistic conditions: past editions of the competition were carried in big conference settings, university campuses and a shopping mall. Positioning accuracy is computed while the person carrying the system under test walks at normal walking speed, uses lifts and goes up and down stairs or briefly stops at given points. Results presented here are a showcase of state-of-the-art systems tested side by side in real-world settings as part of the on-site real-time competition Tracks. Results for off-site Tracks allow a detailed and reproducible comparison of the most recent positioning and tracking algorithms in the same environment as the on-site Tracks

A novel infrared laser device that measures multilateral parameters of stepping performance for assessment of fall risk in elderly individuals [corrected].

Avoiding falls requires fast and appropriate step responses in real-life situations. We developed a step-tracking device that uses an infrared laser sensor for convenient assessment of stepping performance, including concurrent assessment of temporal and spatial parameters. In the present study, we created a new index for assessment of fall risk that uses step speed and accuracy measurements. The purpose of this study was to determine whether the new index could discriminate between elderly individuals with different risks of falling

Preparations of Histone and Nucleosome

In eukaryote cells chromatin is composed of DNA, histone and nonhistone proteins. Among them histone was isolated from chromatin by the replacement with protamine. followed by a gel filtration for the separation of whole histone from the residual protamine. Whole histone was further fractionated to two fractions, A and B. These histone preparations were more native and soluble than that obtained by acid extraction. A DNA-protein complex, nucleosome was prepared by the DNase II digestion of calf thymus chromatin, but perfect digestion to nucleosome monomer could not be carried out. Protein: DNA ratio of this nucleosome (2.07) suggested that nonhistone proteins were also present in nucleosome

Preparations of Histone and Nucleosome

In eukaryote cells chromatin is composed of DNA, histone and nonhistone proteins. Among them histone was isolated from chromatin by the replacement with protamine. followed by a gel filtration for the separation of whole histone from the residual protamine. Whole histone was further fractionated to two fractions, A and B. These histone preparations were more native and soluble than that obtained by acid extraction. A DNA-protein complex, nucleosome was prepared by the DNase II digestion of calf thymus chromatin, but perfect digestion to nucleosome monomer could not be carried out. Protein: DNA ratio of this nucleosome (2.07) suggested that nonhistone proteins were also present in nucleosome

Stress response to laparoscopic liver resection

Background

動物培養細胞の増殖に影響を及ぼす因子に関する研究(I) : 増殖測定簡便法の開発とその応用

We deviced a new method for measurement of cell propagation rate which was more easy and accurate than the conventional "replicate culture method", and discussed on some experimental conditions. At first, the effect of cellular concentration on propagation was studied to determine the optimum concentration at seeding. When MA-30 culture flask was used, the optimum one was 3.2 × 10^4 cells/ml. Then, the effect of medium components was studied and it became apparent that RFL cells could divide at least one time and propagate for 50 hours even in the serum-free synthesized medium. On the other hand, the cells could not propagate but begin to liberate from the glass wall of the culture flask after 6 hour-incubation in a balanced salts solution, PBS. Thus the period of PBS treatment should be restricted within 6 hours. Furthermore, cellular toxicity of dimethyl sulfoxide, DMSO, was tested to know its permissible concentration. Since the treatment with 1 % DMSO for 6 hours had little effect on the propagation of RFL cells, this reagent may be a good solvent in addition tests of water-insoluble substances.従来の重複培養法とくらべ簡便かつ高精度の細胞増殖率測定法を考案し,実施条件について若干の検討を行つた.まず細胞濃度の増殖に及ぼす影響について調べ,細胞まき込み濃度はMA-30型培養瓶を用いる場合は3.2×10^4個/mlが適当であることを見出した.つぎに培養液成分の影響について調べ,細胞は無血清培地中でも50時間にわたり増殖能を維持しうることを明らかにした.一方PBS中では細胞数の減少が認められなかつたのは初期の6時間にすぎず,細胞の生存は比較的短時間しか可能でなかつた.またDMSO添加による影響についても検討し,1%,6時間処理では細胞の増殖に殆んど影響を及ぼさなかつたことから,DMSOが脂溶性化合物添加試験において良好な溶剤となりうることを明らかにした

Study of Factors Affecting Growth of Cultured Mammalian Cells (I) : Development of a Simplified Method of Cell Growth Measurement and Its Aplication

We deviced a new method for measurement of cell propagation rate which was more easy and accurate than the conventional \u22replicate culture method\u22, and discussed on some experimental conditions. At first, the effect of cellular concentration on propagation was studied to determine the optimum concentration at seeding. When MA-30 culture flask was used, the optimum one was 3.2 × 10^4 cells/ml. Then, the effect of medium components was studied and it became apparent that RFL cells could divide at least one time and propagate for 50 hours even in the serum-free synthesized medium. On the other hand, the cells could not propagate but begin to liberate from the glass wall of the culture flask after 6 hour-incubation in a balanced salts solution, PBS. Thus the period of PBS treatment should be restricted within 6 hours. Furthermore, cellular toxicity of dimethyl sulfoxide, DMSO, was tested to know its permissible concentration. Since the treatment with 1 % DMSO for 6 hours had little effect on the propagation of RFL cells, this reagent may be a good solvent in addition tests of water-insoluble substances.従来の重複培養法とくらべ簡便かつ高精度の細胞増殖率測定法を考案し,実施条件について若干の検討を行つた.まず細胞濃度の増殖に及ぼす影響について調べ,細胞まき込み濃度はMA-30型培養瓶を用いる場合は3.2×10^4個/mlが適当であることを見出した.つぎに培養液成分の影響について調べ,細胞は無血清培地中でも50時間にわたり増殖能を維持しうることを明らかにした.一方PBS中では細胞数の減少が認められなかつたのは初期の6時間にすぎず,細胞の生存は比較的短時間しか可能でなかつた.またDMSO添加による影響についても検討し,1%,6時間処理では細胞の増殖に殆んど影響を及ぼさなかつたことから,DMSOが脂溶性化合物添加試験において良好な溶剤となりうることを明らかにした