Physical and Oxidative Stability of Functional olive Oil-in-Water Emulsions Formulated Using Olive Mill Wastewater and Whey Proteins

The present paper reports on the use of phenolic extracts from olive mill wastewater (OMW) in model olive oil-in-water (O/W) emulsions to study their effect on their physical and chemical stability. Spray-dried OMW polyphenols were added to a model 20% olive O/W emulsion stabilized with whey protein isolate (WPI) and xanthan gum, in phosphate buffer solution at pH 7. The emulsions were characterised under accelerated storage conditions (40 °C) up to 30 days. Physical stability was evaluated by analysing the creaming rate, mean particle size distribution and mean droplet size, viscosity and rheological properties, while chemical stability was assessed through the measurement of primary and secondary oxidation products. The rheological behaviour and creaming stability of the emulsions were dramatically improved by using xanthan gum, whereas the concentration of WPI and the addition of encapsulated OMW phenolics did not result in a significant improvement of physical stability. The formation of oxidation products was higher when higher concentrations of encapsulated polyphenols were used, indicating a possible binding with the WPI added in the system as a natural emulsifier. This paper might help in solving the issue of using the olive mill wastewater from olive processing in formulating functional food products with high antioxidant activity and improved health properties

An Assessment of Weather-Related Risks in Europe: Maps of Flood and Drought Risks

This technical report describes the adopted methodology and the outputs produced during the first 18 months of life of the 'ADAM' project. ADAM (Adaptation and Mitigation Strategies: Supporting European Climate Policy) is an Integrated Project financed under thematic priority 'Global Change and Ecosystems' of the 6th framework programme (for further information, see www.adam.info) The task 'A2.1 - An assessment of weather-related risks in Europe' has the following main objective: 'Quantify and map weather-related extreme-event risks to public and private capital assets, human lives, and agriculture/forestry/tourism, and identify high-risk areas (hot spots) on which to focus more detailed analysis.' The key innovative aspects of the work herein presented are manifold: - the quantification of the probabilistic monetary impact of extreme events; - the combined use of modelling techniques and of observed data to supply the lack of information at the various scales of relevance of the study; - the estimation of uncertainty arising from limitations in data availability and modelling assumptions; - the geographical scale (continental) of the exercise. The key outputs of task A2.1 are digital maps of risks from natural extremes at European scale identifying monetary/economic losses. The maps are furnished as input to other tasks of package A2 for successive modelling exercises and analysis. As defined in the project work-plan, task A.21 has duration of 24 months. The 18-month deliverables are maps of flood and drought risks. The report focuses on inland river flood damage to properties and infrastructures and on climatic stresses (drought and heat waves) in agriculture. Population exposure has only been addressed in a partial study and it's therefore not included in the final monetary losses assessment. The work on floods has been carried out by the Institute for Environment and Sustainability of the Joint Research Centre; the work on droughts and heat waves by the Department of Agronomy and Land Management - University of Florence. The methodology is centred on the risk paradigm of the research community. The risk is defined as a product of hazard, exposure and vulnerability where: - Hazard is the threatening natural event including its probability/magnitude of occurrence; - Exposure is the values/humans that are present at the location related to a given event; - Vulnerability is the lack of resistance to damaging/destructive forces (damage function). This definition has been applied to extreme events such as floods and heat/water stresses, with the due adjustments required by data availability and specific modelling techniques.JRC.H.7-Land management and natural hazard

FPGA Implementation of Gaussian Mixture Model Algorithm for 47 fps Segmentation of 1080p Video

Circuits and systems able to process high quality video in real time are fundamental in nowadays imaging systems. The circuit proposed in the paper, aimed at the robust identification of the background in video streams, implements the improved formulation of the Gaussian Mixture Model (GMM) algorithm that is included in the OpenCV library. An innovative, hardware oriented, formulation of the GMM equations, the use of truncated binary multipliers, and ROM compression techniques allow reduced hardware complexity and increased processing capability. The proposed circuit has been designed having commercial FPGA devices as target and provides speed and logic resources occupation that overcome previously proposed implementations. The circuit, when implemented on Virtex6 or StratixIV, processes more than 45 frame per second in 1080p format and uses few percent of FPGA logic resources

Estimation of Vehicle Longitudinal Velocity with Artificial Neural Network

Vehicle dynamics control systems have a fundamental role in smart and autonomous mobility, where one of the most crucial aspects is the vehicle body velocity estimation. In this paper, the problem of a correct evaluation of the vehicle longitudinal velocity for dynamic control applications is approached using a neural networks technique employing a set of measured samples referring to signals usually available on-board, such as longitudinal and lateral acceleration, steering angle, yaw rate and linear wheel speed. Experiments were run on four professional driving circuits with very different characteristics, and the vehicle longitudinal velocity was estimated with different neural network training policies and validated through comparison with the measurements of the one acquired at the vehicle’s center of gravity, provided by an optical Correvit sensor, which serves as the reference (and, therefore, exact) velocity values. The results obtained with the proposed methodology are in good agreement with the reference values in almost all tested conditions, covering both the linear and the nonlinear behavior of the car, proving that artificial neural networks can be efficiently employed onboard, thereby enriching the standard set of control and safety-related electronics

Effect of Salt Addition and Fermentation Time on Phenolics, Microbial Dynamics, Volatile Organic Compounds, and Sensory Properties of the PDO Table Olives of Gaeta (Italy)

‘Oliva di Gaeta’ is almost certainly the most important and well-known PDO denomination for table olives in Italy. Their production is based on a specific two-stage trade preparation called the ‘Itrana’ method. In this work, we investigated how variations in the duration of the initial water fermentation (i.e., 15 and 30 days) and the salt concentration (i.e., 6% and 8% NaCl) influence the chemical features, microbial dynamics, polyphenols, volatile organic compounds, and sensory features of ‘Oliva di Gaeta’. The time of the addition of salt did not affect the final concentration in the brine, but a longer initial water fermentation (before salt addition) led to lower pH values. The bacterial count constantly increased until the salt addition (i.e., either 15 or 30 days), while the yeast population peaked on day 30. Generally, the two different salt concentrations did not affect the count of microorganisms at the end of fermentation, with the only exception being a higher lactic acid bacteria count for the treatment with 6% salt added at 30 days. At commercial maturity, the crucial bitter tastant oleuropein was not completely removed from the drupes, and differences in salt concentration and the length of the first-stage water fermentation did not influence its content at the end of olive curing. Richer volatile profiles of olives were detected with higher-salt treatments, while the combination of low salt and early saline treatment provided a more distinct profile. Longer initial water fermentation caused a small increase in some phenolic compounds (e.g., iso-verbascoside, verbascoside, and hydroxytyrosol-glucoside). A panel test indicated that salt application at 30 days resulted in a more “Sour” and “Bitter” taste, irrespective of the salt concentration. The low salt concentration coupled with the late saline treatment resulted in more “Fruity” notes, probably due to the higher production of esters by lactobacilli. The slightly bitter perception of the olives was consistent with the partial removal of oleuropein. Our work revealed the characteristics of the ‘Itrana’ method and that the variation in salt concentration and its time of application changes parameters ranging from the microbial dynamics to the sensory profile. Specifically, our data indicate that 6% NaCl coupled with a longer initial water fermentation is the most different condition: it is less effective in blocking microbial growth but, at the same time, is more potent in altering the nutritional (e.g., polyphenols) and sensorial qualities (e.g., bitterness and fruitiness) of ‘Oliva di Gaeta’

Proteomic Analysis of Sera from Common Variable Immunodeficiency Patients Undergoing Replacement Intravenous Immunoglobulin Therapy

Common variable immunodeficiency is the most common form of symptomatic primary antibody failure in adults and children. Replacement immunoglobulin is the standard treatment of these patients. By using a differential proteomic approach based on 2D-DIGE, we examined serum samples from normal donors and from matched, naive, and immunoglobulin-treated patients. The results highlighted regulated expression of serum proteins in naive patients. Among the identified proteins, clusterin/ApoJ serum levels were lower in naive patients, compared to normal subjects. This finding was validated in a wider collection of samples from newly enrolled patients. The establishment of a cellular system, based on a human hepatocyte cell line HuH7, allowed to ascertain a potential role in the regulation of CLU gene expression by immunoglobulins

Interest in hearing loss in geriatric medicine: a survey of members of the <em>Societa' Italiana Geriatria Ospedale e Territorio</em> (SIGOT)

Hearing loss is a common condition in older people. Increasing research has shown that this condition is associated with a higher risk of several health problems. The aim of this survey made among the members of the Societa' Italiana Geriatria Ospedale e Territorio (SIGOT) is to explore how far hearing loss is known in the Italian geriatric community and how to develop a collaboration with other services. A short survey (taking approximately 5 min to complete) was freely available on the SIGOT website and disseminated using social channels. The questionnaire was available during the whole year of 2021. The questionnaire specifically addressed general characteristics/ demographics, general attention to hearing loss problem in older people and relationships with the National Health System, and personal opinions regarding hearing loss. A total of 122 participants (mainly females and aging between 61-70 years) from all Italian Regions were included. The SIGOT members answered that they consider hearing loss as clinically relevant is always important (55.7%). Unfortunately, many members had not audiological centers or specialists available. Moreover, 38.5% of SIGOT members reported that the possibility of correction of hearing loss with prostheses or cochlear implants in older people is very good for older patients and that in 87.7% a significant improvement in quality of life was observed. The interest in hearing loss problems in older people perceived by the SIGOT members is elevated, but some barriers, particularly in the availability of other specialists and in the services given by National Health System are still very limited

The TgsGP gene is essential for resistance to human serum in Trypanosoma brucei gambiense

Trypanosoma brucei gambiense causes 97% of all cases of African sleeping sickness, a fatal disease of sub-Saharan Africa. Most species of trypanosome, such as T. b. brucei, are unable to infect humans due to the trypanolytic serum protein apolipoprotein-L1 (APOL1) delivered via two trypanosome lytic factors (TLF-1 and TLF-2). Understanding how T. b. gambiense overcomes these factors and infects humans is of major importance in the fight against this disease. Previous work indicated that a failure to take up TLF-1 in T. b. gambiense contributes to resistance to TLF-1, although another mechanism is required to overcome TLF-2. Here, we have examined a T. b. gambiense specific gene, TgsGP, which had previously been suggested, but not shown, to be involved in serum resistance. We show that TgsGP is essential for resistance to lysis as deletion of TgsGP in T. b. gambiense renders the parasites sensitive to human serum and recombinant APOL1. Deletion of TgsGP in T. b. gambiense modified to uptake TLF-1 showed sensitivity to TLF-1, APOL1 and human serum. Reintroducing TgsGP into knockout parasite lines restored resistance. We conclude that TgsGP is essential for human serum resistance in T. b. gambiense

Dyslipidemia and changes in lipid profiles associated with rheumatoid arthritis and initiation of anti–tumor necrosis factor therapy

Objective To investigate the frequency of lipid testing in clinical practice and to explore the relationship between rheumatoid arthritis (RA), dyslipidemia, and other cardiovascular (CV) risk factors with RA treatment. Methods Patients in this retrospective database study were ages ≥18 years and had ≥2 physician diagnoses for RA or osteoarthritis (OA; comparator group) between March 2004 and March 2008. Outcomes of interest included the percentage of RA and OA patients receiving lipid tests, lipid profiles (total cholesterol, low‐density lipoprotein [LDL] cholesterol, and high‐density lipoprotein [HDL] cholesterol) of RA versus OA patients, and lipid profiles of RA patients before and after initiation with a tumor necrosis factor (TNF) inhibitor. We used multivariable regression to control potential confounders between the cohorts. Results Over a median ≥2‐year followup, fewer RA patients than OA patients had ≥1 lipid test (62.0% [95% confidence interval (95% CI) 61.5–62.5] versus 69.8% [95% CI 69.5–70.1]). Mean total cholesterol and LDL cholesterol were each 4 mg/dl lower in the RA cohort ( P < 0.0001); HDL cholesterol was similar between the cohorts. Across the RA cohort, 25.2% of patients had suboptimal LDL cholesterol levels (≥130 mg/dl). Among RA patients not receiving lipid‐lowering therapy who initiated TNF inhibitor therapy (n = 96), mean total cholesterol and LDL cholesterol increased by 5.4 and 4.0 mg/dl, respectively. Conclusion Patients with RA were less likely to be tested for hyperlipidemia and had more favorable lipid profiles than patients with OA. TNF inhibitor therapy modestly increased all lipid parameters. Additional studies are needed to determine the effect of traditional CV risk factors and inflammation and the impact of biologic agents on CV outcomes in RA patients.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/93521/1/21693_ftp.pd