Structural changes in intestinal enteroendocrine cells after ileal interposition in normal rats

INTRODUCTION: No therapeutic approach has significantly impacted the progression of diabetes. As early improvement of glicaemic control is observed after bariatric surgeries, there is currently a search for surgical procedures that can promote euglycemia also in non-obese patients. Glicaemic control can be achieved by increasing the blood concentration of GLP-1, a hormone produced by L cells that are more densely concentrated in the terminal ileum. The interposition of ileal segment to a more anterior region (proximal jejunum) can promote a greater stimulation of the L cells by poorly digested food, increasing the production of GLP-1 and reflecting on glicaemic control.
AIMS: To investigate long-term histological modifications of intestinal mucosa of rats submitted to interposition of ileum segment to a proximal region (jejunum).
METHODS: Forty 8-week old male Wistar-EPM1 rats (Rattus norvegicus albinus) were randomly distributed into 3 groups: the Interposition Group (IG) was subjected to ileal interposition, the Sham Group (SG) was subjected to sham operations, and the Control Group (CG) was not subjected to surgery. All animals were followed until the 60th postoperative day (8 postoperative week) when they were euthanized. Segments of jejunum and ileum from all groups were collected and analyzed by optical microscopy and immunohistochemistry.
RESULTS: No structural nor histological changes in intestinal L cells in the interposed intestinal segment and other intestinal segments were noted after ileal interposition surgery. 
CONCLUSION: As L cells endocrine characteristics were likely maintained, the use of metabolic surgical techniques for the treatment of metabolic diseases, especially diabetes, seems to be justified

First-year experience of a Brazilian tertiary medical center in supporting severely ill patients using extracorporeal membrane oxygenation

OBJECTIVES: The aim of this manuscript is to describe the first year of our experience using extracorporeal membrane oxygenation support. METHODS: Ten patients with severe refractory hypoxemia, two with associated severe cardiovascular failure, were supported using venous-venous extracorporeal membrane oxygenation (eight patients) or veno-arterial extracorporeal membrane oxygenation (two patients). RESULTS: The median age of the patients was 31 yr (range 14-71 yr). Their median simplified acute physiological score three (SAPS3) was 94 (range 84-118), and they had a median expected mortality of 95% (range 87-99%). Community-acquired pneumonia was the most common diagnosis (50%), followed by P. jiroveci pneumonia in two patients with AIDS (20%). Six patients were transferred from other ICUs during extracorporeal membrane oxygenation support, three of whom were transferred between ICUs within the hospital (30%), two by ambulance (20%) and one by helicopter (10%). Only one patient (10%) was anticoagulated with heparin throughout extracorporeal membrane oxygenation support. Eighty percent of patients required continuous venous-venous hemofiltration. Three patients (30%) developed persistent hypoxemia, which was corrected using higher positive end-expiratory pressure, higher inspired oxygen fractions, recruitment maneuvers, and nitric oxide. The median time on extracorporeal membrane oxygenation support was five (range 3-32) days. The median length of the hospital stay was 31 (range 3-97) days. Four patients (40%) survived to 60 days, and they were free from renal replacement therapy and oxygen support. CONCLUSIONS: The use of extracorporeal membrane oxygenation support in severely ill patients is possible in the presence of a structured team. Efforts must be made to recognize the necessity of extracorporeal respiratory support at an early stage and to prompt activation of the extracorporeal membrane oxygenation team

MANEJO DE CRANIECTOMIA DESCOMPRESSIVA EM NEUROCIRURGIA PEDIÁTRICA

The aim of this article is to provide a comprehensive overview of pediatric decompressive craniectomy, covering from surgical decision-making to post-operative care and reintegration. The multidisciplinary analysis emphasizes the importance of monitoring, pain management, psychosocial support, and continuous education, aiming not only for surgical effectiveness but also the overall well-being of the child for a successful recovery. Methodology: This involves an integrative review with literature search in specialized databases such as PubMed and Scopus, using relevant terms related to decompressive craniectomy and pediatric neurosurgery, descriptors: "Craniectomy," "Decompressive Craniectomy," "Child Health," "Pediatric Care." Results: In the development, the article explores the phases of pediatric decompressive craniectomy, encompassing surgical decision-making, post-operative care, and reintegration. It emphasizes the importance of aspects such as monitoring, pain management, psychosocial support, and continuous education to promote a complete recovery. The integrative approach seeks a holistic understanding, considering both medical and emotional-social aspects. Conclusion: Pediatric decompressive craniectomy requires comprehensive care, including monitoring and emotional support. Successful reintegration is promoted through continuous guidance, covering home care and school adaptations. This approach aims for surgical effectiveness and overall well-being in recovery.O objetivo deste artigo é oferecer uma visão abrangente sobre a craniectomia descompressiva em crianças, abordando desde a decisão cirúrgica até os cuidados pós-operatórios e reintegração. A análise multidisciplinar destaca a importância da monitorização, manejo da dor, apoio psicossocial e educação contínua, visando não apenas a eficácia cirúrgica, mas também o bem-estar global da criança para uma recuperação bem-sucedida. Metodologia: Trata-se de uma revisão integrativa com a busca de literatura em bases de dados especializadas, como PubMed e Scopus, utilizando termos relevantes relacionados à craniectomia descompressiva e neurocirurgia pediátrica, os descritores: “Craniectomia”, “Craniectomia Descompressiva”, “Saúde da Criança”, “Cuidado Infantil”. Resultados: No desenvolvimento, o artigo explora as fases da craniectomia descompressiva pediátrica, abrangendo a decisão cirúrgica, cuidados pós-operatórios e reintegração. Destaca a importância de aspectos como monitorização, manejo da dor, apoio psicossocial e educação contínua para promover uma recuperação completa. A abordagem integrativa busca uma compreensão holística, considerando tanto os aspectos médicos quanto os emocionais e sociais. Conclusão: A craniectomia descompressiva em crianças demanda cuidados abrangentes, incluindo monitorização e suporte emocional. A reintegração bem-sucedida é promovida por meio de orientações contínuas, abrangendo cuidados em casa e adaptações escolares. Essa abordagem visa eficácia cirúrgica e bem-estar total na recuperação

Leucemia Linfoblástica Aguda (LLA) na população pediátrica: marcadores moleculares e implicações terapêuticas

A Leucemia Linfoblástica Aguda (LLA) é uma forma comum de câncer pediátrico, representando cerca de 80% dos casos de leucemia em crianças. A patologia é caracterizada pela proliferação descontrolada de células-tronco hematopoéticas na medula óssea, e avanços recentes na pesquisa genômica têm proporcionado uma compreensão mais profunda da complexidade molecular subjacente à doença. O presente estudo tem como objetivo oferecer uma visão abrangente dos principais marcadores moleculares e implicações terapêuticas associadas à LLA na população pediátrica. Este estudo, baseado em uma revisão sistemática da literatura científica, abrange o período de 2013 a 2023, utilizando as bases de dados PubMed (Medline), Cochrane Library e Scientific Electronic Library Online (SciELO). Marcadores moleculares preponderantes, como rearranjos cromossômicos específicos (t(12;21), t(1;19), t(9;22)), mutações genéticas distintivas (ETV6-RUNX1, E2A-PBX1, TP53) e amplificação do gene BCR-ABL1, têm sido objeto de estudo aprofundado. Esses marcadores desempenham um papel crucial na estratificação de risco e prognóstico, permitindo uma abordagem mais personalizada no tratamento da LLA em crianças. As implicações terapêuticas derivadas desses marcadores são vastas, destacando a promissora era das terapias direcionadas. Terapias específicas para mutações, como aquelas direcionadas à mutação BCR-ABL1, e inovações em imunoterapia estão moldando o cenário do tratamento da LLA, proporcionando resultados mais eficazes e menos tóxicos. Os resultados destacam a eficácia das terapias direcionadas e a necessidade contínua de pesquisa para otimizar a intervenção terapêutica, melhorar a qualidade de vida dos pacientes pediátricos afetados pela LLA e explorar novas facetas do tratamento. Em conclusão, este artigo fornece uma análise aprofundada dos marcadores moleculares e terapias associadas à LLA na população pediátrica, destacando avanços significativos e delineando áreas para investigação futura

Effects of hospital facilities on patient outcomes after cancer surgery: an international, prospective, observational study

Background Early death after cancer surgery is higher in low-income and middle-income countries (LMICs) compared with in high-income countries, yet the impact of facility characteristics on early postoperative outcomes is unknown. The aim of this study was to examine the association between hospital infrastructure, resource availability, and processes on early outcomes after cancer surgery worldwide.Methods A multimethods analysis was performed as part of the GlobalSurg 3 study-a multicentre, international, prospective cohort study of patients who had surgery for breast, colorectal, or gastric cancer. The primary outcomes were 30-day mortality and 30-day major complication rates. Potentially beneficial hospital facilities were identified by variable selection to select those associated with 30-day mortality. Adjusted outcomes were determined using generalised estimating equations to account for patient characteristics and country-income group, with population stratification by hospital.Findings Between April 1, 2018, and April 23, 2019, facility-level data were collected for 9685 patients across 238 hospitals in 66 countries (91 hospitals in 20 high-income countries; 57 hospitals in 19 upper-middle-income countries; and 90 hospitals in 27 low-income to lower-middle-income countries). The availability of five hospital facilities was inversely associated with mortality: ultrasound, CT scanner, critical care unit, opioid analgesia, and oncologist. After adjustment for case-mix and country income group, hospitals with three or fewer of these facilities (62 hospitals, 1294 patients) had higher mortality compared with those with four or five (adjusted odds ratio [OR] 3.85 [95% CI 2.58-5.75]; p<0.0001), with excess mortality predominantly explained by a limited capacity to rescue following the development of major complications (63.0% vs 82.7%; OR 0.35 [0.23-0.53]; p<0.0001). Across LMICs, improvements in hospital facilities would prevent one to three deaths for every 100 patients undergoing surgery for cancer.Interpretation Hospitals with higher levels of infrastructure and resources have better outcomes after cancer surgery, independent of country income. Without urgent strengthening of hospital infrastructure and resources, the reductions in cancer-associated mortality associated with improved access will not be realised

De Amsterdamse Raadhuisprijsvraag. Een polemiek in kaart gebracht.

In 1936 schreef de gemeente Amsterdam een prijsvraag uit voor het nieuw te bouwen raadhuis aan het Frederiksplein. Op deze prijsvraag en de ingezonden resultaten werd gereageerd in de media. Het doel van dit onderzoek, dat is uitgevoerd in het kader van een bachelorscriptie, is om de polemiek omtrent deze prijsvraag in kaart te brengen gedurende de jaren 1936 tot en met 1940. Hiervoor is onderzoek gedaan in twee kranten en twee architectuurtijdschriften, namelijk het Algemeen Handelsblad, De Telegraaf, Bouwkundig Weekblad Architectura en het R.K. Bouwblad. Deze bronnen zijn digitaal doorzocht. Het blijkt dat veel auteurs zich teleurgesteld tonen over de prijsvraag. Deze teleurstelling is te verdelen in vier thema’s. Allereerst is er veel aan te merken op het gekozen bouwterrein. Daarnaast kunnen de ontwerpen die de jury gekozen heeft niet op ieders goedkeuring rekenen. Verder is ook de jury een punt van discussie en tenslotte is niet iedereen het eens met het verloop van de prijsvraag. Opvallend is dat de teleurstelling nauwelijks in het Bouwkundig Weekblad geuit wordt. Dit tijdschrift was het officiële orgaan van de Bond van Nederlandse Architecten en de redactie komt regelmatig in het harnas tegen de kritiek die op de prijsvraag wordt geuit. Helaas bleken niet alle edities van genoemde media volledig online te staan en omwille van de grootte van dit onderzoek was het niet mogelijk om uitgebreid archiefonderzoek te verrichten. Vermoedelijk wordt wel de kern van de polemiek behandeld, maar details zouden verder ingevuld kunnen worden als de overige edities ook gedigitaliseerd zouden worden. Ook zou het interessant kunnen zijn om het onderzoek naar de polemiek uit te breiden naar andere media, zoals de Nieuwe Rotterdamsche Courant

OS CUIDADOS DE ENFERMAGEM AO CLIENTE COM HIV/AIDS EM UM HOSPITAL UNIVERSITÁRIO NA DÉCADA DE 1980

RESUMO: Investigação sobre as estratégias dos enfermeiros para configurar os cuidados de enfermagem aos clientes com HIV/Aids no Hospital Universitário Gaffrée e Guinle, da Universidade Federal do Estado do Rio de Janeiro, no período de 1983-1987. Objetivos: analisar as estratégias empreendidas pelos enfermeiros diante do desafio de cuidar dos clientes com HIV/Aids nesse Hospital; e, discutir a eficácia simbólica dessas estratégias para o reconhecimento da importância do enfermeiro no cuidado ao cliente com HIV/Aids. Estudo histórico-social; com nove enfermeiros que se envolveram com os cuidados aos clientes com HIV/Aids. Resultados: evidenciamos que o pouco conhecimento sobre o HIV/Aids, o medo e a discriminação foram os principais entraves para os cuidados de enfermagem aos clientes, nos primeiros anos da epidemia, nas enfermarias do Hospital, mas os enfermeiros não se abateram, foram em busca da (re) atualização do habitus. Conclusão: a partir desse momento, os enfermeiros tornaram-se porta-vozes do cuidado aos clientes com HIV/Aids e começaram a reorganizar o cuidado de enfermagem nas enfermarias desse Hospital, o que possibilitou ofertar aos clientes com HIV/Aids um cuidado humanizado, digno e pautado no respeito ao ser humano e na valorização da vida. Descritores: História da enfermagem; cuidados de enfermagem; enfermagem

Resposta defensiva a potenciais predadores de camundongos Swiss expostos a água contendo resíduos de cigarro

Não há