INFLUÊNCIA DAS CONDIÇÕES DE SECAGEM NAS PROPRIEDADES FUNCIONAIS DA Aphanothece microscopica Nägeli CULTIVADA NO EFLUENTE DA PARBOILIZAÇÃO DO ARROZ

O objetivo deste trabalho foi caracterizar a solubilidade e a capacidade emulsificante da biomassa da cianobactéria Aphanothece microscopica Nägeli em diferentes condições de secagem. Produziu-se a biomassa na água residuária do processo de parboilização do arroz, inoculando 100 mg.L-1 de Aphanothece microscopica Nägeli na fase exponencial de crescimento em reator cilíndrico de mistura completa, na ausência de luz, pH 8,0, 30ºC, razão C/N 50 e tempo de detenção hidráulica de 72h. A biomassa foi separada do efluente por centrifugação e desidratada em secador descontínuo de bandejas nas condições de 40, 50 e 60ºC em espessuras de biomassa de 3, 5 e 7 mm. A solubilidade protéica foi avaliada em pH 2, 3, 4, 6, 8, 9 e 10 e a capacidade emulsificante determinada em pH 7,0. Os resultados demonstraram que as maiores solubilidades protéicas ocorreram em pH 10, havendo incremento da capacidade emulsificante nas camadas de biomassa de 3 mm de espessura

In search of a tolerance-induction strategy for cow's milk allergies: significant reduction of beta-lactoglobulin allergenicity via transglutaminase/cysteine polymerization

OBJECTIVE: To explore the use of β-lactoglobulin polymerized using microbial transglutaminase and heating to identify whether protein polymerization could reduce in vivo allergenicity and maintain in vitro and ex vivo immunoreactivity for use in tolerance-induction protocols. METHODS: Based on previous protocols applied in mice and children, we performed in vivo challenges (using a skin prick test) with native and polymerized β-lactoglobulin in adult patients with an IgE-mediated allergy to plactoglobulin. In vitro humoral immunoreactivity was analyzed using immunoblotting. Cell-mediated immunoreactivity was analyzed using ex vivo challenges with native and polymerized β-lactoglobulin and monitored by leukocyte adherence inhibition tests. RESULTS: The skin tests demonstrated that there was a significant reduction in immediate cutaneous reactivity after polymerization. Polymerization did not decrease the immunoblotting detection of s-IgE specific to β-lactoglobulin. Cell-mediated immunoreactivity, as assessed by ex vivo challenges and leukocyte adherence inhibition tests, did not exhibit significant differences between leukocytes challenged with native versus polymerized β-lactoglobulin. CONCLUSIONS: The polymerization of β-lactoglobulin decreased in vivo allergenicity and did not decrease in vitro humoral or ex vivo cell-mediated immunoreactivity. Therefore, we conclude that inducing polymerization using transglutaminase represents a promising technique to produce suitable molecules for the purpose of designing oral/ sublingual tolerance induction protocols for the treatment of allergies

Social distancing measures to control the COVID-19 pandemic: potential impacts and challenges in Brazil.

The COVID-19 pandemic has challenged researchers and policy makers to identify public safety measures forpreventing the collapse of healthcare systems and reducingdeaths. This narrative review summarizes the available evidence on the impact of social distancing measures on the epidemic and discusses the implementation of these measures in Brazil. Articles on the effect of social distancing on COVID-19 were selected from the PubMed, medRXiv and bioRvix databases. Federal and state legislation was analyzed to summarize the strategies implemented in Brazil. Social distancing measures adopted by the population appear effective, particularly when implemented in conjunction with the isolation of cases and quarantining of contacts. Therefore, social distancing measures, and social protection policies to guarantee the sustainability of these measures, should be implemented. To control COVID-19 in Brazil, it is also crucial that epidemiological monitoring is strengthened at all three levels of the Brazilian National Health System (SUS). This includes evaluating and usingsupplementary indicators to monitor the progression of the pandemic and the effect of the control measures, increasing testing capacity, and making disaggregated notificationsand testing resultstransparentand broadly available

Estudos Artísticos

O 18º número da revista croma apresenta 15 artigos que prosseguem os objetivos editoriais desta revista. Trata-se de desafiar os autores ou criadores apresentar a obra de outros artistas preferencialmente originários dos países de expressão portuguesa ou espanhola.info:eu-repo/semantics/publishedVersio

Uso do teste LF-LAM para diagnóstico de Tuberculose ativa em paciente que vive com HIV

A tuberculose (TB) é uma doença infectocontagiosa granulomatosa, de alta infectividade e baixa patogenicidade, de tropismo primariamente respiratório, mas que pode afetar outros órgãos, como linfonodos, pele, rins, intestino e cérebro. É imperativo excluir o diagnóstico de TB em pacientes diagnosticados com HIV. Este estudo se trata do relato de caso de um paciente de 30 anos, gênero masculino, sem comorbidades prévias, que comparece à Unidade de Pronto Atendimento (UPA) queixando-se de desconforto respiratório aos pequenos esforços, tosse seca, febrícula aferida diariamente, diaforese, disfagia e perda ponderal de 15 kg. Feita a suspeição clínica de tuberculose, optou-se pela solicitação do teste rápido molecular para TB, no entanto, o paciente não conseguiu produzir a amostra necessária para análise. Assim, foi solicitado o teste de antígeno urinário lipoarabinomanano, que foi positivo, permitindo o tratamento precoce dessa grave infecção. Confirmou-se, também, o diagnóstico de HIV. O desconhecimento de métodos diagnósticos alternativos para pacientes de alto risco para TB é um complexo desafio em saúde pública, dada a elevada taxa de morbimortalidade nos portadores dessa doença, sobretudo, naqueles coinfectados com o vírus HIV

Acceptability and preference drivers of red wines produced from vitis labrusca and hybrid grapes

Wines produced from non-Vitis vinifera varieties have great economic importance in Brazil and represent more than 80% of the national production, but scientific information regarding the quality of these wines is rare. The objective of this research was to determine consumer acceptability, the sensory profile and the chemical composition of the most consumed Brazilian red wines produced with Vitis labrusca and promising hybrid varieties, identifying the parameters that drive the preference of consumers. Commercial wines collected directly from different wineries were evaluated regarding their overall acceptance by 120 consumers. Twelve trained panelists developed the sensory profile of the wines using Quantitative Descriptive Analysis (QDA). Physicochemical analyses carried out to determine the pH, titratable and volatile acidity, total solids, alcohol degree, total phenolics, free SO2 and the reducing sugar contents of the wines. The data was analyzed by ANOVA, Tukey test, Internal Preference Mapping (MDPREF), Cluster analysis, Principal Component Analysis (PCA), Partial Least Square regression (PLS), and Extended Internal Preference Map (EPM). In general wines produced from Ives (V. labrusca) grape showed higher aroma/flavor notes described as sweet, grape, grape juice, blackberry and roses. The wines produced from the hybrid grape Máximo differed from those elaborated with the variety Ives, especially due to their higher intensity of earthy/mushroom, vegetative/green beans, woody and yeast sensory notes. The PLS and EPM analyses indicated that fruity notes associated with the aroma and flavor of grape and grape juice were sensory drivers of Brazilian consumers' preference. On the other hand, the majority of the consumers did not like the sensory notes described as earthy/mushroom, vegetative/green beans and yeast found in the wines produced from Máximo. The wine elaborated exclusively with the hybrid Seibel 2 and the wine containing the V. labrusca grapes Ives and Isabella were preferred by the majority of the consumers and only a minor segment of consumers appreciated the wines elaborated solely with Máximo grape. Thus the hybrid grape Seibel 2 and the recently developed hybrid Máximo were shown to be promising varieties for the winemaking of quality wines in Brazilian regions where the environmental conditions are not good for the cultivation of V. vinifera varieties62456466CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQ131017/2006-