Han River Chulmuntogi: A Study of Early Neolithic Korea

Han River Chulmuntogi: A Study of Early Neolithic Korea by Sarah M. Nelson: Scattered throughout the Korean peninsula are about 100 known sites which contain handmade pottery decorated with parallel incised lines. First described by Japanese archaeologists in the early part of this century, the pottery was thought to resemble Central European ceramics decorated with a multiple toothed tool, and so was designated in Japanese Kushimemon Doki, a translation of Kammkeramik (comb-pattern pottery). Translated into Korean, the same characters are pronounced Chulmuntogi, and sites with such pottery are known collectively in Korea as the Chulmuntogi Munhwa, the comb-pattern pottery culture. Although not descriptive of a majority of the pottery called Chulmun, this term will nevertheless be retained here since it has become established in Korea. It is well understood to cover a variety of other decorative techniques as well as possible comb markings on prehistory pottery in Korea.https://cedar.wwu.edu/easpress/1025/thumbnail.jp

Neutralizing monoclonal antibodies block human immunodeficiency virus type 1 infection of dendritic cells and transmission to T cells

Prevention of the initial infection of mucosal dendritic cells (DC) and interruption of the subsequent transmission of HIV-1 from DC to T cells are likely to be important attributes of an effective human immunodeficiency virus type 1 (HIV-1) vaccine. While anti-HIV-1 neutralizing antibodies have been difficult to elicit by immunization, there are several human monoclonal antibodies (MAbs) that effectively neutralize virus infection of activated T cells. We investigated the ability of three well-characterized neutralizing MAbs (IgG1b12, 2F5, and 2G12) to block HIV-1 infection of human DC. DC were generated from CD14+ blood cells or obtained from cadaveric human skin. The MAbs prevented viral entry into purified DC and the ensuing productive infection in DC/T-cell cultures. When DC were first pulsed with HIV-1, MAbs blocked the subsequent transmission to unstimulated CD3+ T cells. Thus, neutralizing antibodies can block HIV-1 infection of DC and the cell-to-cell transmission of virus from infected DC to T cells. These data suggest that neutralizing antibodies could interrupt the initial events associated with mucosal transmission and regional spread of HIV-1

Characterization of Metabolic, Diffusion, and Perfusion Properties in GBM: Contrast-Enhancing versus Non-Enhancing Tumor.

BackgroundAlthough the contrast-enhancing (CE) lesion on T1-weighted MR images is widely used as a surrogate for glioblastoma (GBM), there are also non-enhancing regions of infiltrative tumor within the T2-weighted lesion, which elude radiologic detection. Because non-enhancing GBM (Enh-) challenges clinical patient management as latent disease, this study sought to characterize ex vivo metabolic profiles from Enh- and CE GBM (Enh+) samples, alongside histological and in vivo MR parameters, to assist in defining criteria for estimating total tumor burden.MethodsFifty-six patients with newly diagnosed GBM received a multi-parametric pre-surgical MR examination. Targets for obtaining image-guided tissue samples were defined based on in vivo parameters that were suspicious for tumor. The actual location from where tissue samples were obtained was recorded, and half of each sample was analyzed for histopathology while the other half was scanned using HR-MAS spectroscopy.ResultsThe Enh+ and Enh- tumor samples demonstrated comparable mitotic activity, but also significant heterogeneity in microvascular morphology. Ex vivo spectroscopic parameters indicated similar levels of total choline and N-acetylaspartate between these contrast-based radiographic subtypes of GBM, and characteristic differences in the levels of myo-inositol, creatine/phosphocreatine, and phosphoethanolamine. Analysis of in vivo parameters at the sample locations were consistent with histological and ex vivo metabolic data.ConclusionsThe similarity between ex vivo levels of choline and NAA, and between in vivo levels of choline, NAA and nADC in Enh+ and Enh- tumor, indicate that these parameters can be used in defining non-invasive metrics of total tumor burden for patients with GBM

Effects of Foster Care Placements on the Mental Health of Abused Children in Florida

INTRODUCTION: As evidenced in the literature, when maltreated children are admitted into state care, they are frequently not afforded sufficient mental health treatment. Concomitantly, foster parents are often not given proper training in providing complex care for these children. As such, the current study aimed to examine the role that foster care has in the development of psychopathology in maltreated children and their caregivers. METHODS: Participants included 234 maltreated youths (ages 7 to 17) presenting for treatment at a community mental health center specializing in childhood trauma. Children and adolescents currently residing in foster care as well as in their biological home environments were included. RESULTS: Results of multiple regression models indicated that a history of foster care plays a significant role in the association between children and adolescents who have witnessed domestic violence and internalizing disorders, externalizing disorders, and parenting stress. DISCUSSION: Results from this study revealed that a lack of foster care history plays a significant role in moderating the development of psychopathology in children and adolescents who have witnessed domestic violence. This association was also found with parenting stress. Future research needs to further explicate the specific roles that a child’s living situation can play in future psychological impairment

Reflecting on the Past

150 years of Augustana storieshttps://digitalcommons.augustana.edu/ahsreflecting/1000/thumbnail.jp

Reflecting on the Past

150 years of Augustana storieshttps://digitalcommons.augustana.edu/ahsreflecting/1000/thumbnail.jp

Common Sense for Caring Organizations: Results from a Study of High-Performing Home Care Agencies and Nursing Homes

This study reports on results of a qualitative study of 21 high-performing Ohio nursing homes and home care agencies. The study focused on best practices for managing their direct care workforce to achieve high performance. The report includes the most prevalent practice themes as well as tips and management strategies

Measurement of Differentially Methylated INS DNA Species in Human Serum Samples as a Biomarker of Islet β Cell Death

The death of islet β cells is thought to underlie the pathogenesis of virtually all forms of diabetes and to precede the development of frank hyperglycemia, especially in type 1 diabetes. The development of sensitive and reliable biomarkers of β cell death may allow for early therapeutic intervention to prevent or delay the development of diabetes. Recently, several groups including our own have reported that cell-free, differentially methylated DNA encoding preproinsulin (INS) in the circulation is correlated to β cell death in pre-type 1 diabetes and new-onset type 1 diabetes. Here, we present a step-by-step protocol using digital PCR for the measurement of cell-free INS DNA that is differentially methylated at cytosine at position -69 bp (relative to the transcriptional start site). We demonstrate that the assay can distinguish between methylated and unmethylated cytosine at position -69 bp, is linear across several orders of magnitude, provides absolute quantitation of DNA copy numbers, and can be applied to samples of human serum from individuals with new-onset type 1 diabetes and disease-free controls. The protocol described here can be adapted to any DNA species for which detection of differentially methylated cytosines is desired, whether from circulation or from isolated cells and tissues, and can provide absolute quantitation of DNA fragments

Vemurafenib-resistant BRAF-V600E-mutated melanoma is regressed by MEK-targeting drug trametinib, but not cobimetinib in a patient-derived orthotopic xenograft (PDOX) mouse model.

Melanoma is a recalcitrant disease. The present study used a patient-derived orthotopic xenograft (PDOX) model of melanoma to test sensitivity to three molecularly-targeted drugs and one standard chemotherapeutic. A BRAF-V600E-mutant melanoma obtained from the right chest wall of a patient was grown orthotopically in the right chest wall of nude mice to establish a PDOX model. Two weeks after implantation, 50 PDOX nude mice were divided into 5 groups: G1, control without treatment; G2, vemurafenib (VEM) (30 mg/kg); G3; temozolomide (TEM) (25 mg/kg); G4, trametinib (TRA) (0.3 mg/kg); and G5, cobimetinib (COB) (5 mg/kg). Each drug was administered orally, daily for 14 consecutive days. Tumor sizes were measured with calipers twice a week. On day 14 from initiation of treatment, TRA, an MEK inhibitor, was the only agent of the 4 tested that caused tumor regression (P < 0.001 at day 14). In contrast, another MEK inhibitor, COB, could slow but not arrest growth or cause regression of the melanoma. First-line therapy TEM could slow but not arrest tumor growth or cause regression. The patient in this study had a BRAF-V600E-mutant melanoma and would be considered to be a strong candidate for VEM as first-line therapy, since VEM targets this mutation. However, VEM was not effective. The PDOX model thus helped identify the very-high efficacy of TRA against the melanoma PDOX and is a promising drug for this patient. These results demonstrate the powerful precision of the PDOX model for cancer therapy, not achievable by genomic analysis alone