Enhancing normal labour by adopting antenatal physiotherapy: a prospective study

Background: In the recent past there had been a steady rise in caesarean section rate as against the vaginal delivery. Information is still scarce on the benefits of physiotherapy and birthing experience of women who exercises in antenatal duration. Benefits of exercise during pregnancy include; prevention of excessive gestational weight gain, gestational diabetes, pre-eclampsia, decrease need for caesarean section and reduced low back pain. The objective of this study was to evaluation of antenatal physiotherapy on maternal and perinatal outcome and subjective evaluation of degree of labour pain perception.Methods: In the study 150 primigravida subjects in second to third trimester without any associated high risk factor and were willing to exercise during pregnancy were chosen.Results: In the study group, the average duration of latent phase [7.56 (±2.02) hours] and active phase of first stage of labour [3.26 (±1.06) hours] was significantly lesser as compared to the control group, 89.3% (n=134) delivered vaginally and 10.7% (n=16) subjects had to be taken for caesarean section. In the study group no perineal tear or need for any instrumentation (outlet forceps/ventouse) to facilitate delivery was seen. Higher proportion of the study group subjects i.e. 70.9% experienced moderate to mild pain during labour. and majority i.e. 72% subjects delivered newborns with birth weight >2.5 kg,with higher one minute and five minutes APGAR scores.Conclusions: This highlights the very need for physiotherapy to be inculcated into the curriculum during antenatal period with conducive maternal and foetal outcome as it inflates the chances of normal deliveries and abate the rates of cesarean section and subsequent, which is the dire need of the day

Phytotoxic effect of the extracts of Parthenium hysterophorus L. on the germination, seedling growth and biomass of some agricultural crops

This study was conducted to assess the phytotoxic effect of invasive species: Parthenium hysterophorus L. on the germination, seedling length and biomass of Zea mays, Macrotyloma uniflorum and Triticum aestivum, in order to explore its potential as noxious species. In this study, the allelopathic substances were extracted with distilled water and methanol from the aerial and underground part of P. hysterophorus L. in different concentration (1, 100, 150, 400 mg mL–1). The results of the study revealed that the methanol fraction of roots at 400 mg mL-1 concentration had the highest phytotoxic activity as compared to the double distilled water extract. Germination (%) was observed to be maximum (90%) for Z. mays with double distilled water above ground part extract at 1 mg mL-1 and lowest (10%) for M. uniflorum L. in 400 mg mL-1 both aerial and underground plant extracts at the last day of measurement. Mean shoot and root length were observed highest (17.6cm and 6.1cm respectively) in Z. mays with aerial double distilled water extract at 1 mg mL-1 concentration and it was found lowest (3.2cm and 2.8cm) in M. uniflorum with underground part double distilled water extract at 400 mg mL-1. Biomass was recorded highest (1.97 g/100cm2) in Z. mays in aerial part extract and lowest (0.83 g/100 cm2) in horse gram in underground part extract at concentration of 400mg mL–1 with double distilled water extract. In methanol, both aerial and underground part extracts, the germination observed only at 1mg mL-1 concentration

Ecological Studies of the River Krishna near Gadwal, Telangana with Reference to Water Quality

Ecological studies in the river Krishna have been studies for a period of two years (May 2015 to April 2017). Physico-chemical and phycological aspects have been investigated to assess the quality of water. The water was well oxygenated with an alkaline pH, organic matter, chlorides and nutrients were recorded at low concentrations. Diatoms constituted the dominant group of algae. Multiple regression analysis was employed to discover the relative importance of various physico-chemical variables on the abundance and distribution of algae. Mathematical equations were derived involving the physico-chemical variables for better prediction of algal number

A signature motif mediating selective interactions of BCL11A with the NR2E/F subfamily of orphan nuclear receptors

Despite their physiological importance, selective interactions between nuclear receptors (NRs) and their cofactors are poorly understood. Here, we describe a novel signature motif (F/YSXXLXXL/Y) in the developmental regulator BCL11A that facilitates its selective interaction with members of the NR2E/F subfamily. Two copies of this motif (named here as RID1 and RID2) permit BCL11A to bind COUP-TFs (NR2F1;NR2F2;NR2F6) and Tailless/TLX (NR2E1), whereas RID1, but not RID2, binds PNR (NR2E3). We confirmed the existence of endogenous BCL11A/TLX complexes in mouse cortex tissue. No interactions of RID1 and RID2 with 20 other ligand-binding domains from different NR subtypes were observed. We show that RID1 and RID2 are required for BCL11A-mediated repression of endogenous γ-globin gene and the regulatory non-coding transcript Bgl3, and we identify COUP-TFII binding sites within the Bgl3 locus. In addition to their importance for BCL11A function, we show that F/YSXXLXXL/Y motifs are conserved in other NR cofactors. A single FSXXLXXL motif in the NR-binding SET domain protein NSD1 facilitates its interactions with the NR2E/F subfamily. However, the NSD1 motif incorporates features of both LXXLL and FSXXLXXL motifs, giving it a distinct NR-binding pattern in contrast to other cofactors. In summary, our results provide new insights into the selectivity of NR/cofactor complex formation

Factors Associated with Mortality in Coronavirus-Associated Mucormycosis: Results from Mycotic Infections in COVID-19 (MUNCO) Online Registry

Background: COVID-19-associated mucormycosis (CAM) is associated with high morbidity and mortality. MUNCO is an international database used to collect clinical data on cases of CAM in real time. Preliminary data from the Mycotic Infections in COVID-19 (MUNCO) online registry yielded 728 cases from May to September 2021 in four South Asian countries and the United States. A majority of the cases (694; 97.6%) consisted of a mucormycosis infection. The dataset allowed for the analysis of the risk factors for adverse outcomes from CAM and this analysis is presented in this paper. Methods: The submission of cases was aided by a direct solicitation and social media online. The primary endpoints were full recovery or death measured on day 42 of the diagnosis. All patients had histopathologically confirmed CAM. The groups were compared to determine the contribution of each patient characteristic to the outcome. Multivariable logistic regression models were used to model the probability of death after a CAM diagnosis. Results: The registry captured 694 cases of CAM. Within this, 341 could be analyzed as the study excluded patients with an unknown CAM recovery status due to either an interruption or a lack of follow up. The 341 viable cases consisted of 258 patients who survived after the completion of treatment and 83 patients who died during the period of observation. In a multivariable logistic regression model, the factors associated with an increased risk of mortality include old age (OR = 1.04, 95% CI 1.02–1.07, p = 0.001), history of diabetes mellitus (OR 3.5, 95% CI 1.01–11.9, p = 0.02) and a lower BMI (OR 0.9, 95% CI 0.82–0.98, p = 0.03). Mucor localized to sinus disease was associated with 77% reduced odds of death (OR = 0.23, 95% CI 0.09–0.57, p = 0.001), while cerebral mucor was associated with an increased odds of death (OR = 10.96, 95% CI 4.93–24.36, p = ≤0.0001). Conclusion: In patients with CAM, older age, a history of diabetes and a lower body mass index is associated with increased mortality. Disease limited to the sinuses without a cerebral extension is associated with a lower risk of mortality. Interestingly, the use of zinc and azithromycin were not associated with increased mortality in our study

Laboratory preparedness for SARS-CoV-2 testing in India: Harnessing a network of Virus Research & Diagnostic Laboratories

BACKGROUND & OBJECTIVES: An outbreak of respiratory illness of unknown aetiology was reported from Hubei province of Wuhan, People's Republic of China, in December 2019. The outbreak was attributed to a novel coronavirus (CoV), named as severe acute respiratory syndrome (SARS)-CoV-2 and the disease as COVID-19. Within one month, cases were reported from 25 countries. In view of the novel viral strain with reported high morbidity, establishing early countrywide diagnosis to detect imported cases became critical. Here we describe the role of a countrywide network of VRDLs in early diagnosis of COVID-19. METHODS: The Indian Council of Medical Research (ICMR)-National Institute of Virology (NIV), Pune, established screening as well as confirmatory assays for SARS-CoV-2. A total of 13 VRDLs were provided with the E gene screening real-time reverse transcription-polymerase chain reaction (rRT-PCR) assay. VRDLs were selected on the basis of their presence near an international airport/seaport and their past performance. The case definition for testing included all individuals with travel history to Wuhan and symptomatic individuals with travel history to other parts of China. This was later expanded to include symptomatic individuals returning from Singapore, Japan, Hong Kong, Thailand and South Korea. RESULTS: Within a week of standardization of the test at NIV, all VRDLs could initiate testing for SARS-CoV-2. Till February 29, 2020, a total of 2,913 samples were tested. This included both 654 individuals quarantined in the two camps and others fitting within the case definition. The quarantined individuals were tested twice - at days 0 and 14. All tested negative on both occasions. Only three individuals belonging to different districts in Kerala were found to be positive. INTERPRETATION & CONCLUSIONS: Sudden emergence of SARS-CoV-2 and its potential to cause a pandemic posed an unsurmountable challenge to the public health system of India. However, concerted efforts of various arms of the Government of India resulted in a well-coordinated action at each level. India has successfully demonstrated its ability to establish quick diagnosis of SARS-CoV-2 at NIV, Pune, and the testing VRDLs

An Epidemiological Analysis of SARS-CoV-2 Genomic Sequences from Different Regions of India

The number of Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) cases is increasing in India. This study looks upon the geographic distribution of the virus clades and variants circulating in different parts of India between January and August 2020. The NPS/OPS from representative positive cases from different states and union territories in India were collected every month through the VRDLs in the country and analyzed using next-generation sequencing. Epidemiological analysis of the 689 SARS-CoV-2 clinical samples revealed GH and GR to be the predominant clades circulating in different states in India. The northern part of India largely reported the ‘GH’ clade, whereas the southern part reported the ‘GR’, with a few exceptions. These sequences also revealed the presence of single independent mutations—E484Q and N440K—from Maharashtra (first observed in March 2020) and Southern Indian States (first observed in May 2020), respectively. Furthermore, this study indicates that the SARS-CoV-2 variant (VOC, VUI, variant of high consequence and double mutant) was not observed during the early phase of virus transmission (January–August). This increased number of variations observed within a short timeframe across the globe suggests virus evolution, which can be a step towards enhanced host adaptation