Long-Term Safety of Tedizolid in a Patient With Spondilodiscitis After Switch From Linezolid Due to Toxicity

The patient is a 57-year-old man with liver cirrhosis, Bricker anastomosis after a radical cystoprostatectomy and, a history of bacteremias caused by extended-spectrum -lactamase-positive Escherichia coli, Enterococcus faecium, and Candida albicans. He presented with persistent low back pain and was diagnosed with vertebral osteomyelitis, for which he received ertapenem-linezolid treatment. However, after 20 days, linezolid had to be discontinued because of myelotoxicity and metabolic acidosis. The patient was switched to tedizolid, which, in combination with ertapenem, was successfully given for 114 days until biopsy showed no growth of gram-positive cocci. We conclude that tedizolid can be an alternative to linezolid in case of toxicity, especially in long-term treatments

Current profiles and AC losses of a superconducting strip with elliptic cross-section in perpendicular magnetic field

The case of a hard type II superconductor in the form of strip with elliptic cross-section when placed in transverse magnetic field is studied. We approach the problem in two steps, both based on the critical-state model. First we calculate numerically the penetrated current profiles that ensure complete shielding in the interior, without assuming an a priori form for the profiles. In the second step we introduce an analytical approximation that asumes that the current profiles are ellipses. Expressions linking the sample magnetization to the applied field are derived covering the whole range of applied fields. The theoretical predictions are tested by the comparison with experimental data for the imaginary part of AC susceptibility.Comment: 12 pages; 3 figure

Caracterización y principales patologías de la arenisca “Piedra Dorada” empleada en los edificios históricos de las ciudades de Úbeda y Baeza (Jaén, Sur de España)

La arenisca conocida como “Piedra Dorada” es uno de los materiales más empleados en la construcción de gran parte de los edificios históricos de la provincia de Jaén. Un ejemplo de ello se encuentra en las ciudades de Úbeda y Baeza, declaradas Patrimonio Cultural de la Humanidad por la Unesco. Se ha realizado una caracterización detallada de la roca, en sus principales variedades: “Viva”, “Franca” y “Jabaluna”. Ha sido analizado tanto material de las canteras originales como muestras de monumentos. En el presente trabajo se determinan las principales características mineralógicas, texturales y geoquímicas así como las propiedades físicas de las variedades de “Piedra Dorada”. Además se profundiza en el estudio de las principales patologías encontradas en los edificios históricos de las ciudades anteriormente citadas. A partir de los diferentes parámetros determinados se ha establecido un orden de calidad de las variedades de “Piedra Dorada”. También se han caracterizado los mecanismos de deterioro entre los que se encuentran: colonización biológica (líquenes, algas, musgos y plantas superiores), ascenso capilar, depósitos superficiales (sales y costras negras) y deterioros debidos a factores antrópicos como fugas en la red de alcantarillado, ausencia de cubiertas y bajantes, etc. Este estudio ha permitido conocer en detalle una de las piedras de construcción más importantes de Andalucía con el fin de garantizar su perdurabilidad a través de los años. The sandstone known as “Piedra Dorada”, or golden stone, is one of the most commonly used materials in the construction of many of the main historic buildings in the province of Jaén. Many examples can be found in the cities of Úbeda and Baeza, declared World Heritage Cities by UNESCO. A thorough characterization of their main varieties: “Viva”, “Franca” and “Jabaluna” has been done. Original quarry materials as well as samples of monuments have been analyzed. In this study we present a characterization of the mineralogical, textural and geochemistry features as well as the main physical properties of the varieties of “Piedra Dorada”. Furthermore, this research investigates in depth the main pathologies found in historic buildings in the cities mentioned above. We have established an order of quality of the varieties mentioned from the different parameters determined. We have also characterized the deterioration mechanisms amongst which are: biological colonization (lichens, algae, moss and higher plants), an increase in capillary action, surface deposits (salts and black crust) and anthropic factors such as leaks into the sewer network, absence of covers and downspouts, etc. This study has allowed us to know in detail one of the most important construction stones of Andalusia in order to ensure its durability for future years

Conservation status of Narcissus perezlarae Font Quer (Amaryllidaceae) in the Valencian Community

Narcissus perezlarae está incluido en el Catálogo Valenciano de Especies de Flora Amenazadas de la Comunidad Valenciana (Orden 6/2013) en la categoría "En peligro de extinción". Estudios recientes han demostrado su origen híbrido (= N. cavanillesii × N. miniatus [= N. serotinus auct.]). Por otro lado, el mayor especialista y monografía del género reclamó recientemente su autonomía como especie independiente, como N. piifontianus. Esta especie es endémica de la península Ibérica, presente en Alto Alentejo (Portugal), Sevilla y Cádiz (España), y en la parte oriental de la Cordillera Bética, entre Alicante y Valencia. Sin embargo, si se tratara como una especie independiente, sería un endemismo exclusivamente restringido a la Comunidad Valenciana. Siete poblaciones naturales han sido monitoreadas, cuyos datos censales oscilan anualmente. El último censo contiene 1.634 plantas. Desde 2010, se han llevado a cabo cinco experiencias de translocación en la provincia de Alicante. De un total de 8.970 ejemplares introducidos se ha registrado una tasa de supervivencia del 28,4% (2.545 plantas) en el último censo. Las poblaciones recién creadas aumentan el número de localidades conocidas de la especie en la Comunidad Valenciana y amplían considerablemente su distribución a lo largo del territorio.Narcissus perezlarae is included in the Valencian Catalogue of Threatened Species of the Valencian Community (Order 6/2013) in the category "In danger of extinction". Recent studies have demonstrated its hybridogenic origin (= N. cavanillesii × N. miniatus [= N. serotinus auct.]). On the other hand, the greatest specialist and monograph of the genus has most recently claimed its autonomy as an independent species, as N. piifontianus. This species is endemic to the Iberian Peninsula, present in Alto Alentejo (Portugal), Sevilla and Cadiz (Spain), and in the eastern part of the Baetic Mountains, between Alicante and Valencia. However, if treated as an independent species, it would be an endemism exclusively restricted to the Valencian Community. Seven natural populations have been monitored, whose census data oscillate annually. The latest census contains 1634 plants. Since 2010, five translocation experiences have been carried out in Alicante province. A total amount of 8,970 plants were translocated and a survival rate of 28,4% (2,545 plants) was observed in the latest census. The newly created populations increase the number of known locations of the species in the Valencian Community and considerably expand their distribution range along the territory

Protocolo de sedo-analgesia para prevención del espasmo radial en hemodinámica cardíaca

Introducción y objetivo: El espasmo es la complicación más habitual en los cateterismos por arteria radial. Su frecuencia oscila entre el 10-30% y puede ser un factor limitante que impida la realización del cateterismo por esa vía. El objetivo de este estudio es evaluar con un nuevo protocolo de sedo-analgesia la reducción de la frecuencia del espasmo radial y la disminución de la ansiedad del paciente. Material y método: Estudio aleatorizado y prospectivo de 300 pacientes sometidos a cateterismo radial. Se randomizaron dos grupos, el Grupo I (n=150) con la pauta de sedación habitual (10mg diazepam sl) y el Grupo II (n=150) con una pauta de sedación con 2 mg de Midazolam + 0,035 mg/kg de Cloruro Mórfico y en caso de procedimientos de más de 45 minutos se añadía Fentanilo a 1 mcgr/kg. Resultados y conclusión: No se observaron diferencias significativas entre los dos grupos estudiados en cuanto a las características basales. La edad media de la población fue de 65 ± 11 años; 223 pacientes (74%) fueron hombres y el índice de masa corporal (IMC) medio 27,7 ± 3,8. Los pacientes del Grupo II presentaron reducción significativa del espasmo respecto a los del Grupo I (9,3% frente a 22,6%; p=0,002). También se objetivó una reducción significativa del dolor (2,05 frente a 2,77; p=0,007). La pauta sedo-analgésica propuesta demostró ser eficaz en la reducción del espasmo radial y del dolor durante el cateterismo

Body composition, nutritional profile and muscular fitness affect bone health in a sample of schoolchildren from Colombia: The Fuprecol Study

This is the final version of the article. Available from the publisher via the DOI in this record.The objective of the present study is to investigate the relationships between body composition, nutritional profile, muscular fitness (MF) and bone health in a sample of children and adolescents from Colombia. Participants included 1118 children and adolescents (54.6% girls). Calcaneal broadband ultrasound attenuation (c-BUA) was obtained as a marker of bone health. Body composition (fat mass and lean mass) was assessed using bioelectrical impedance analysis. Furthermore height, weight, waist circumference and Tanner stage were measured and body mass index (BMI) was calculated. Standing long-jump (SLJ) and isometric handgrip dynamometry were used respectively as indicators of lower and upper body muscular fitness. A muscular index score was also computed by summing up the standardised values of both SLJ and handgrip strength. Dietary intake and degree of adherence to the Mediterranean diet were assessed by a 7-day recall questionnaire for food frequency and the Kidmed questionnaire. Poor bone health was considered using a z-score cut off of ≤−1.5 standard deviation. Once the results were adjusted for age and Tanner stage, the predisposing factors of having a c-BUA z-score ≤−1.5 standard deviation included being underweight or obese, having an unhealthy lean mass, having an unhealthy fat mass, SLJ performance, handgrip performance, and unhealthy muscular index score. In conclusion, body composition (fat mass and lean body mass) and MF both influenced bone health in a sample of children and adolescents from Colombia. Thus promoting strength adaptation and preservation in Colombian youth will help to improve bone health, an important protective factor against osteoporosis in later life.: We would like to acknowledge to Bogota District Education Department for supporting data collection for this study. The authors also thank the participating Bogota District students, teachers, schools, and staff. The “FUPRECOL Study” was possible given the financial support provided by the Instituto Colombiano para el Desarrollo de la Ciencia y la Tecnología “Francisco José de Caldas” COLCIENCIAS (Contract Number 671-2014 Code 122265743978). Also, during the completion of this paper, AG-H was visiting researchers at the University of Rosario (CEMA, Bogotá, Colombia), supported with grants awarded by Proyectos Basales y Vicerrectoría de Investigación, Desarrollo e Innovación” (Universidad de Santiago de Chile, USACH)

Accelerated Variant of Idiopathic Pulmonary Fibrosis: Clinical Behavior and Gene Expression Pattern

Idiopathic pulmonary fibrosis (IPF) is characterized by the insidious onset of dyspnea or cough. However, a subset of patients has a short duration of symptoms with rapid progression to end-stage disease. In this study, we evaluated clinical and molecular features of "rapid" and "slow" progressors with IPF

Immune-related gene expression profiling after PD-1 blockade in non–small cell lung carcinoma, head and neck squamous cell carcinoma, and melanoma

Antibody targeting of the immune checkpoint receptor PD1 produces therapeutic activity in a variety of solid tumors, but most patients exhibit partial or complete resistance to treatment for reasons that are unclear. In this study, we evaluated tumor specimens from 65 patients with melanoma, lung nonsquamous, squamous cell lung or head and neck cancers who were treated with the approved PD1-targeting antibodies pembrolizumab or nivolumab. Tumor RNA before anti-PD1 therapy was analyzed on the nCounter system using the PanCancer 730-Immune Panel, and we identified 23 immune-related genes or signatures linked to response and progression-free survival (PFS). In addition, we evaluated intra- and interbiopsy variability of PD1, PD-L1, CD8A, and CD4 mRNAs and their relationship with tumor-infiltrating lymphocytes (TIL) and PD-L1 IHC expression. Among the biomarkers examined, PD1 gene expression along with 12 signatures tracking CD8 and CD4 T-cell activation, natural killer cells, and IFN activation associated significantly with nonprogressive disease and PFS. These associations were independent of sample timing, drug used, or cancer type. TIL correlated moderately (~0.50) with PD1 and CD8A mRNA levels and weakly (~0.35) with CD4 and PD-L1. IHC expression of PD-L1 correlated strongly with PD-L1 (0.90), moderately with CD4 and CD8A, and weakly with PD1. Reproducibility of gene expression in intra- and interbiopsy specimens was very high (total SD <3%). Overall, our results support the hypothesis that identification of a preexisting and stable adaptive immune response as defined by mRNA expression pattern is reproducible and sufficient to predict clinical outcome, regardless of the type of cancer or the PD1 therapeutic antibody administered to patients

A direct physical interaction between Nanog and Sox2 regulates embryonic stem cell self-renewal

Embryonic stem (ES) cell self-renewal efficiency is determined by the Nanog protein level. However, the protein partners of Nanog that function to direct self-renewal are unclear. Here, we identify a Nanog interactome of over 130 proteins including transcription factors, chromatin modifying complexes, phosphorylation and ubiquitination enzymes, basal transcriptional machinery members, and RNA processing factors. Sox2 was identified as a robust interacting partner of Nanog. The purified Nanog–Sox2 complex identified a DNA recognition sequence present in multiple overlapping Nanog/Sox2 ChIP-Seq data sets. The Nanog tryptophan repeat region is necessary and sufficient for interaction with Sox2, with tryptophan residues required. In Sox2, tyrosine to alanine mutations within a triple-repeat motif (S X T/S Y) abrogates the Nanog–Sox2 interaction, alters expression of genes associated with the Nanog-Sox2 cognate sequence, and reduces the ability of Sox2 to rescue ES cell differentiation induced by endogenous Sox2 deletion. Substitution of the tyrosines with phenylalanine rescues both the Sox2–Nanog interaction and efficient self-renewal. These results suggest that aromatic stacking of Nanog tryptophans and Sox2 tyrosines mediates an interaction central to ES cell self-renewal