Gene action and component of genetic variance analysis in the thermo sensitive genetic male sterile (TGMS) line in rice (Oryza sativa L.)

The field experiment conducted with eighteen TGMS lines, seventy two F1’s and four checks in randomized complete block design with three replications at the Norman E. Borlaug Crop Research Centre of Govind Ballabh Pant University of Agriculture and Technology, Pantnagar during Kharif 2013 and Kharif 2014. Estimates of variance of general combining ability were lower than those of variance of specific combining ability for all the traits e.g panicle number per plant (0.54), 1000 grain weight (1.50), harvest index (3.83) and grain yield per plant (17.09) showing preponderance of non-additive gene action except panicle length (1.28) and spikelet number per panicle(522.70) which showed high gca and indicated additive gene action. High estimates of broad sense heritability coupled with high genetic advance in mean percentage for Spikelet number per panicle (98 and 27.23 respectively). For grain yield per plant (98 and 14.38) and grain number per panicle (97 and 19.93) high heritability coupled with moderate genetic advance (98 and 14.38) indicated the scope of getting better recombinants. Selection will be more effective for the traits those have high heritability and high genetic advance

Inter-relationship and path analysis of different traits of two line hybrid in rice (Oryza sativa L.)

The present study was undertaken with the objective to determine the nature and magnitude of variability, degree of association between yield and it`s component characters and their direct and indirect effects on grain yield in rice. The experiment was conducted on rice in year 2013-14 at Borlaug Crop Research Center of G. B. Pant University of Agriculture and Technology, Pantnagar, Uttarakhand. All the traits had positive correlation to seed yield per plant of rice except days to flowering at genotypic level and at the phenotypic level. At phenotypic level panicle length (0.2145) and harvest index (0.4713) had highly significant level and positive correlation to seed yield per plant. Grain number per panicle (0.1550) and panicle number per plant (0.1398) showed positive and level of significant correlation with seed yield per plant. The days to flowering (-0.1879) have highly significant level with negative correlation to seed yield per plant. Path analysis showed the positive and direct effect on seed yield per plant at genotypic and phenotypic level for panicle number per plant (0.393 and 0.380 respectively), panicle length (0.236 and 0.198), grain number per panicle (0.646 and 0.112) and harvest index (0.443 and 0.448). While days to flowering (-0.175 and -0.167) and plant height (-0.037 and -0.008) had negative direct effect. The traits which showed positive correlation and positive direct effect on seed yield per plant can be used to increase seed yield for further breeding programs and may be given due importance in selection during rice breeding programme

Primera cita de Botrytis cinerea sobre Lilium polyphyllum, especie en peligro de extinción en Uttarakhand, India

Dhyani, A. High Altitude Plant Physiology Research Centre. Srinagar, Pauri Garhwal, Uttarakhand, India.Nautiyal, B. P. Department of Horticulture. Aromatic and Medicinal Plant, Mizoram University, Aizawl, India.Nautiyal, M. C. High Altitude Plant Physiology Research Centre. Srinagar, Pauri Garhwal, Uttarakhand, India.Rivera, Marta Carolina. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Producción Vegetal. Cátedra de Fitopatología. Buenos Aires, Argentina.Prasad, D. University of Agriculture and Technology. College of Forestry and Hill Agriculture.Plant Pathology Section. Ranichauri, India.Singh, K. P. University of Agriculture and Technology. College of Forestry and Hill Agriculture.Plant Pathology Section. Ranichauri, India.157-159Lilium polyphyllum es una planta herbácea que se encuentra en estado de peligro en ambientes naturales de Uttarakhand, India. La enfermedad conocida como moho gris fue detectada sobre esta especie desde Julio a Agosto (2008-2009) causando muerte de inflorescencias y varas. Botrytis cinerea fue aislado consistentemente de inflorescencias. Su patogenicidad fue confirmada mediante inoculación de plantas de L. polyphyllum cultivadas en macetas en un invernáculo. Como resultado, se cita a Botrytis cinerea por primera vez como causante de moho gris sobre Lilium polyphyllum en India, cuyos síntomas son tizón de flores y varas. Esta enfermedad puede afectar seriamente a este hospedante en riesgo durante su estado reproductivo. A nuestro entender, no existe otro antecedente de B. cinerea sobre este hospedante

Cultivation of Angelica archangelica Linn.: evaluation for economical viability at two different climatic conditions

ABSTRACT Cultivation of an important sub alpine-alpine medicinal and aromatic herb, Angelica archangelica Linn. (Apiaceae), was carried out at two different climatic zone at 2200 m asl (Pothivasa, PV) and 3600 m asl (Tungnath, TN) altitudes in Garhwal, North west Himalaya, India. These altitudes represent temperate and alpine region of the Himalaya. Since the species has a very few wild populations, cultivation of the species is recommended for its medicinal use. After the establishment of sufficient quantity of seedlings, they were transplanted in both sites at the age of two, three and four months for the comparative accounts on survival, growth and yield and, to test the appropriate age of seedlings for transplantation and climatic suitability for cultivation of the species. Organic cultivation protocol was adopted by using different farm yard manure (FYM) as treatments. Yield was minimum in youngest seedlings (two months old ) transplanted at TN in ordinary alpine soil (control) and maximum under polyhouse beds at PV after three years of growth. Observations on yield at different climatic zone revealed that it was maximum in PV (temperate site) as compared to alpine site (natural site) in all treatments. Seedlings of different age also had better survival, growth and yield at the temperate site though; two months old seedlings after transplantation had comparatively slow growth and yield than those of three and four months seedlings. These observations clearly suggested that temperate region (2200 m) is suitable for cultivation of A. archangelica. Addition of manure (leaf litter in particular) and polyhouse cultivation further improved the yield. Economical viability of the cultivation was also observed and presented here

Ecological features and traditional knowledge of Roscoea alpina Royle a medicinal plant in Himalaya

167-173Roscoea alpina Royle (Zingiberaceae) commonly known as Kakoli is a perennial medicinal plant endemic to the Himalayas. It has been used in vitality strengthening Ayurveda groups i.e., Astavarga and Jeevaniyagana and in preparation of Ayurvedic formulations e.g., Chyavanprasha and Divya Pidantak Tail. Considering its medicinal uses, high demand and overexploitation, natural habitats were surveyed in subalpine and alpine regions of Garhwal Himalaya for population estimation and to identify elite germplasm. R. alpina frequency was recorded more than 60% in Tungnath, Dayara, Valley of Flowers and Kedarnath populations. However, plant density and area occupied were low compared to other species of subalpine and alpine site. For threat category assessment, IUCN Red List Categories and Criteria were used and conservation status was assigned, based on site to site and for entire Garhwal region of the Western Himalaya. R. alpina, based on extent of occurrence was categorized as Vulnerable and based on number of mature individuals as Endangered for the Garhwal Himalaya. Further, habitat destruction and degradation were the major threats for population reduction in the wild. Morphological variation revealed plants from Tungnath and Kedarnath may be used for future propagation and domestication programs

NK cells augment oncolytic adenovirus cytotoxicity in ovarian cancer

Oncolytic viruses (OVs) can trigger profound innate and adaptive immune responses, which have the potential both to potentiate and reduce the activity of OVs. Natural killer (NK) cells can mediate potent anti-viral and anti-tumoral responses, but there are no data on the role of NK cells in oncolytic adenovirus activity. Here, we have used two different oncolytic adenoviruses—the Ad5 E1A CR2-deletion mutant dl922-947 (group C) and the chimeric Ad3/Ad11p mutant enadenotucirev (group B)—to investigate the effect of NK cells on overall anti-cancer efficacy in ovarian cancer. Because human adenoviruses do not replicate in murine cells, we utilized primary human NK cells from peripheral blood and ovarian cancer ascites. Our results show that dl922-947 and enadenotucirev do not infect NK cells, but induce contact-dependent activation and anti-cancer cytotoxicity against adenovirus-infected ovarian cancer cells. Moreover, manipulation of NK receptors DNAM-1 (DNAX accessory molecule-1) and TIGIT (T cell immunoreceptor with Ig and ITIM domains) significantly influences NK cytotoxicity against adenovirus-infected cells. Together, these results indicate that NK cells act to increase the activity of oncolytic adenovirus in ovarian cancer and suggest that strategies to augment NK activity further via the blockade of inhibitory NK receptor TIGIT could enhance therapeutic potential of OVs

Effect of seed moisture content and storage temperature on seed longevity of hemp (Cannabis sativa)

Hemp (Cannabis sativa L.) is one of the earliest domesticated plants grown for its protein and oil rich seed, fiber and psychoactive substances and it is one of the earliest known medicinal plants in human history. Studies were conducted on seed germinability (germination test) and viability (topographical tetrazoliun chloride test) in three seed lots to determine the seed quality. Studies conducted on effect of five seed moisture contents (5, 7, 8, 10 and 12 % on fresh weight basis), three storage temperature (ambient, 15°C and -20°C) and eight storage periods (0, 3, 6, 9, 12, 18, 24 and 36 months) on seed longevity revealed that the critical moisture content (moisture content required in seeds for retaining initial germination after storage of seeds up to 36 months) of seeds for ambient storage condition of Delhi was 5 %, which increased to 7 % in 15°C and 12 % at -20°C storage temperature. The seeds are desiccation as well as chilling tolerant, therefore, exhibit orthodox storage behavior and are ideal for ex-situ conservation of seeds in seed/gene banks

Bacterial Endophytes Enhance Competition by Invasive Plants

Premise of the study: Invasive plants can alter soil microbial communities and profoundly alter ecosystem processes. In the invasive grass Sorghum halepense, these disruptions are consequences of rhizome-associated bacterial endophytes. We describe the effects of N2-fixing bacterial strains from S. halepense (Rout and Chrzanowski, 2009) on plant growth and show that bacteria interact with the plant to alter soil nutrient cycles, enabling persistence of the invasive. Methods: We assessed fluxes in soil nutrients for ~4 yr across a site invaded by S. halepense. We assayed the N2-fixing bacteria in vitro for phosphate solubilization, iron chelation, and production of the plant-growth hormone indole-3-acetic acid (IAA). We assessed the plant’s ability to recruit bacterial partners from substrates and vertically transmit endophytes to seeds and used an antibiotic approach to inhibit bacterial activity in planta and assess microbial contributions to plant growth. Key results: We found persistent alterations to eight biogeochemical cycles (including nitrogen, phosphorus, and iron) in soils invaded by S. halepense. In this context, three bacterial isolates solubilized phosphate, and all produced iron siderophores and IAA in vitro. In growth chamber experiments, bacteria were transmitted vertically, and molecular analysis of bacterial community fingerprints from rhizomes indicated that endophytes are also horizontally recruited. Inhibiting bacterial activity with antibiotics resulted in significant declines in plant growth rate and biomass, with pronounced rhizome reductions. Conclusions: This work suggests a major role of endophytes on growth and resource allocation of an invasive plant. Indeed, bacterial isolate physiology is correlated with invader effects on biogeochemical cycles of nitrogen, phosphate, and iron