L’évolution de la polygamie en milieu rural sénégalais : institution en crise ou en mutation ?

On annonce depuis longtemps le déclin de la polygamie en Afrique. Pourtant, ces pratiques restent courantes dans de nombreux pays de la région, surtout en milieu rural. Depuis les années 1970, la crise économique et agricole a entraîné de profonds changements dans l’organisation des ménages et laisse entrevoir une remise en question des fondements de la polygamie. L’étude de l’évolution de la polygamie dans la zone d’étude de Niakhar, au Sénégal, met en évidence la complexité des forces en jeu : bien que les niveaux restent stables au cours du temps, les hommes des générations récentes entrent en première union polygame moins rapidement que leurs aînés. En combinant des données qualitatives avec celles d’une enquête biographique rétrospective, cette étude vise à identifier quels processus influent sur le calendrier d’entrée en union polygame des hommes au fil des générations. La confrontation des différentes données laisse croire à l’adoption d’attitudes visant la redéfinition des fondements de la polygamie plutôt que sa remise en question, redéfinition ayant pour objectif d’en assurer la pérennité.The decline of polygyny in sub-Saharan Africa has been predicted for decades but the levels remain high in many countries, especially in rural areas. Since the 1970s, the economic and agricultural crisis has strongly affected household organization, suggesting that the bases for polygamy might be in question. The study of polygamous behaviours in the Niakhar rural area in Senegal highlights the different forces at work: despite relatively stable levels in recent decades, younger men are entering their first polygamous union later than their elders. By combining qualitative data with the results of a retrospective biographical survey, this study seeks to identify the processes that affect the timing of men’s first polygamous union across different cohorts. The results suggest an adoption of new attitudes, involving a redefinition of the bases for polygamy (rather than a questioning of polygamy), in order to ensure the continuation of such practices

Reporting results back in Health and demographic surveillance systems (HDSS): an ethical requirement and a strategy for improving health behaviours

This paper addresses the issue of reporting results back in Health and demographic surveillance systems (HDSS). In these particular research platforms, populations are constantly solicited through the longitudinal demographic follow-up and additional surveys. Therefore, reporting results back directly to participants should be considered as a strong ethical requirement. However, like in most health oriented research, results are mostly disseminated among decision makers and local authorities. Therefore, HDSS residents increasingly question the objectives of these studies. Using a participatory approach, 3 days were organized in 2015 to report back findings based on 50 years of research on population, health and environment in the Niakhar HDSS in Senegal. Drawing from the evaluation conducted among a sample of participants to the event, we show that beyond the ethical dimension, such activities may also contribute to change populations’ attitudes to research practices and further influence individuals’ health behaviors at the local level.

Separation Methods hyphenated to Mass Spectrometry for the Characterization of the Protein Glycosylation at the Intact Level

International audienceGlycosylation is one of the most common post-translational modifications of proteins that affects their biological activity, solubility, and half-life. Therefore, its characterization is of great interest in proteomic, particularly from a diagnostic and therapeutic point of view. However, the number and type of glycosylation sites, the degree of site occupancy and the different possible structures of glycans can lead to a very large number of isoforms for a given protein, called glycoforms. The identification of these glycoforms constitutes an important analytical challenge. Indeed, to attempt to characterize all of them, it is necessary to develop efficient separation methods associated with a sensitive and informative detection mode, such as mass spectrometry (MS). Most analytical methods are based on bottom-up proteomics, which consists in the analysis of the protein at the glycopeptides level after its digestion. Even if this approach provides essential information, including the localization and composition of glycans on the protein, it is also characterized by a loss of information on macro-heterogeneity, i.e. the nature of the glycans present on a given glycoform. The analysis of glycoforms at the intact level can overcome this disadvantage. The aim of this review is to detail the state-of-the art of separation methods that can be easily hyphenated with MS for the characterization of protein glycosylation at the intact level. The different electrophoretic and chromatographic approaches are discussed in detail. The miniaturization of these separation methods is also discussed with their potential applications. While the first studies focused on 2 the development and optimization of the separation step to achieve high resolution between isoforms, the recent ones are much more application-oriented, such as clinical diagnosis, quality control, and glycoprotein monitoring in formulations or biological samples

Extraction sélective de la cocaïne d'échantillons biologiques avec un polymère à empreintes moléculaires monolithique et miniaturisé et une analyse en ligne par nano-chromatographie en phase liquide

International audienceWe report the on-line coupling of a monolithic molecularly imprinted polymer to nano-liquid chromatography for the selective analysis of cocaine and its main metabolite, benzoylecgonine, in complex biological samples. After the screening of different synthesis conditions, a monolithic molecularly imprinted polymer was in situ synthesized into a 100 µm internal diameter fused-silica capillary using cocaine as template, methacrylic acid as functional monomer, and trimethylolpropane trimethacrylate as cross-linker. Scanning electron microscopy was used to assess the homogeneous morphology of the molecularly imprinted polymer and its permeability was measured. Its selectivity was evaluated by nano-liquid chromatography-ultraviolet, leading to imprinting factors of 3.2 ± 0.5 and 2.2 ± 0.3 for cocaine and benzoylecgonine, respectively, on polymers resulting from three independent syntheses, showing the high selectivity and the repeatability of the synthesis. After optimizing the extraction protocol to promote selectivity, the monolithic molecularly imprinted polymer was successfully on-line coupled with nano-liquid chromatography-ultraviolet for the direct extraction and analysis of cocaine present in spiked human plasma and saliva samples. The repeatability of the obtained extraction recovery, between 85.4-98.7% for a plasma sample spiked at 100 ng.mL-1 , was high with relative standard deviation values lower than 5.8% for triplicate analyses on each of the three independently synthesized molecularly imprinted polymers. A linear calibration range was achieved between 100 and 2000 ng mL-1 (R 2 = 0.999). Limits of quantification of 14.5 ng mL-1 and 6.1 ng mL-1 were achieved in plasma and urine samples, respectively. The very clean-baseline of the resulting chromatogram illustrated the high selectivity brought by the monolithic molecularly imprinted polymer that allows the removal of a huge peak corresponding to the elution of interfering compounds and the easy determination of the target analyte in these complex biological samples

Characterization of phenolic profile alterations in metal-polluted bee pollen via capillary electrophoresis

International audienceBee pollen is a conglomerate of plant pollens, and its nutritional contents include bioactive compounds with antioxidant/antiradical potentials. These potentials are conditioned by botanical origin. In Chile, the native flora is diverse and promising in terms of bioactive compounds, but many plants grow in metal-polluted areas. The associated bioaccumulation could negatively affect the antioxidant/antiradical abilities of bee pollen. To assess the relationship between the bioaccumulation of metals and the antioxidant activity of pollen, complete bee pollen was collected near and far from the Llaima Volcano, albeit in ranges that ensured the same botanical origins. Mellisopalynological analysis determined Escallonia rubra pollen was the most abundant native flora in complete bee pollen. Therefore, E. rubra pollen collected near and far from the Llaima Volcano was evaluated for the following: phenolic compounds via colorimetric assays; antioxidant activity via ferric reducing/antioxidant power assays; antiradical activity via 1,1-diphenyl-2-picrylhydrazyl radical assays; and metal contents via inductively coupled plasma optical emission spectrometry. Llaima samples had higher Cu and Fe but lower Mn contents and lower antioxidant and antiradical capacities than did the control samples. These results were supported by subsequent fortification assays in Llaima E. rubra samples. In fortified samples with significantly higher metal contents, antiradical and antioxidant abilities decreased. Moreover, shifts in migration times were found for naringenin, rutine, and caffeic acid after capillary electrophoresis (CE) analysis in fortified samples. In conclusion, the results indicated an inverse correlation between metal contents and antioxidant/antiradical potentials in bee pollen

Study of mechanical behavior on single use bags welding under gamma irradiation

Since a long time, biopharmaceutical industry utilizes more and more single use plastic bags due to its very easy use (long shelf-lives, mechanical properties), preparation, and storage properties (oxygen and water barriers). These plastic bags are composed of two welded multilayer polymer films. To ensure the function of the closure and the non-contamination from the external environment, welding must answer to several parameters according to norm (“ISO 15747,” 2018) and standard (F02 Committee, n.d.). In this present study, the behavior of weldings on Ethylene Vinyl Acetate (EVA) single use bags under gamma irradiation have been studied. Mechanical tests have been performed at several gamma irradiation doses (from 0 kGy to 270 kGy) and at different location of the bag (Figure 1). The first objective is to study the impact of gamma irradiation dose on the welding mechanical tensile behavior. The second objective is to evaluate the impact of the welding location on the welding tensile properties. Each tensile curve (Figure 2) has been decomposed in 6 characteristic points which were evaluated with Principal Component Analysis (PCA): Ultimate Tensile strength at break (UTS), Ultimate elongation or elongation at break, 1st Yield-Strength (Y1 Strength), 1st Yield-Strain (Y1 Strain), 2nd Yield-Strength (Y2 Strength), 2nd Yield-Strain (Y2 Strain). The study showed that weldings are never impacted during tensile testing: this evaluation reveals that in fine the film cracks before the welding modification. Its function of closure and bag content preservation from external environment is fully achieved whatever the gamma irradiation dose and the welding location. Only the multilayer film on both sides of the welding is altered after 100% elongation strain. The EVA bag showed no degradation up to 115 kGy whereas they become to be altered at 270 kGy. The welding location on EVA bag showed different film mechanical behavior correlated to the polymer film extrusion process orientation. Please click Additional Files below to see the full abstract

Failure of hydrogenation in protecting polycyclic aromatic hydrocarbons from fragmentation

A recent study of soft X-ray absorption in native and hydrogenated coronene cations, C$_{24}$H$_{12+m}^+$ $m=0-7$, led to the conclusion that additional hydrogen atoms protect (interstellar) Polycyclic Aromatic Hydrocarbon (PAH) molecules from fragmentation [Reitsma et al., Phys. Rev. Lett. 113, 053002 (2014)]. The present experiment with collisions between fast (30-200 eV) He atoms and pyrene (C$_{16}$H$_{10+m}^+$, $m=0$, 6, and 16) and simulations without reference to the excitation method suggests the opposite. We find that the absolute carbon-backbone fragmentation cross section does not decrease but increases with the degree of hydrogenation for pyrene molecules.Comment: 10 pages, 5 figure

Ex vivo activity of the ACT new components pyronaridine and piperaquine in comparison with conventional ACT drugs against isolates of Plasmodium falciparum

<p>Abstract</p> <p>Background</p> <p>The aim of the present work was to assess i) <it>ex vivo </it>activity of pyronaridine (PND) and piperaquine (PPQ), as new components of artemisinin-based combination therapy (ACT), to define susceptibility baseline, ii) their activities compared to other partner drugs, namely monodesethylamodiaquine (MDAQ), lumefantrine (LMF), mefloquine (MQ), artesunate (AS) and dihydroartemisinin (DHA) against 181 <it>Plasmodium falciparum </it>isolates from African countries, India and Thailand, and iii) <it>in vitro </it>cross-resistance with other quinoline drugs, chloroquine (CQ) or quinine (QN).</p> <p>Methods</p> <p>The susceptibility of the 181 <it>P. falciparum </it>isolates to the nine anti-malarial drugs was assessed using the standard 42-hours ³H-hypoxanthine uptake inhibition method.</p> <p>Results</p> <p>The IC₅₀values for PND ranged from 0.55 to 80.0 nM (geometric mean = 19.9 nM) and from 11.8 to 217.3 nM for PPQ (geometric mean = 66.8 nM). A significant positive correlation was shown between responses to PPQ and PND responses (<it>rho </it>= 0.46) and between PPQ and MDAQ (<it>rho </it>= 0.30). No significant correlation was shown between PPQ IC₅₀and responses to other anti-malarial drugs. A significant positive correlation was shown between responses to PND and MDAQ (<it>rho </it>= 0.37), PND and LMF (<it>rho </it>= 0.28), PND and QN (<it>rho </it>= 0.24), PND and AS (<it>rho </it>= 0.19), PND and DHA (<it>rho </it>= 0.18) and PND and CQ (<it>rho </it>= 0.16). All these coefficients of correlation are too low to suggest cross-resistance between PPQ or PND and the other drugs.</p> <p>Conclusions</p> <p>In this study, the excellent anti-malarial activity of PPQ and PND was confirmed. The absence of cross-resistance with quinolines and artemisinin derivatives is consistent with the efficacy of the combinations of PPQ and DHA or PND and AS in areas where parasites are resistant to conventional anti-malarial drugs.</p