Learning for Transformation of Water Governance: Reflections on Design from the Climate Change Adaptation and Water Governance (CADWAGO) Project

This paper considers how learning for transformation of water governance in the context of climate change adaptation can be designed for and supported, drawing examples from the international climate change adaptation and water governance project (CADWAGO). The project explicitly set out to design for governance learning in the sense of developing elements of social infrastructure such as workshops, performances and online media to bring stakeholders together and to facilitate co-learning of relevance to governance. CADWAGO drew on a variety of international cases from past and ongoing work of the project partners. It created a forum for dialogue among actors from different contexts working at different levels and scales. The range of opportunities and constraints encountered are discussed, including the principles and practicalities of working with distributed processes of design and leadership of events. A range of concepts, tools and techniques were used to consider and facilitate individual and collective learning processes and outcomes associated with water governance in the context of climate adaptation. Questions were addressed about how elements of past, present and future water governance thinking and practice are connected and how multi-level systemic change in governance can take place. Some reflections onthe effectiveness of the design for learning process are included. The nature of the contribution that projects such as CADWAGO can make in learning for transformation of water governance practices is also critically considered

Finishing the euchromatic sequence of the human genome

The sequence of the human genome encodes the genetic instructions for human physiology, as well as rich information about human evolution. In 2001, the International Human Genome Sequencing Consortium reported a draft sequence of the euchromatic portion of the human genome. Since then, the international collaboration has worked to convert this draft into a genome sequence with high accuracy and nearly complete coverage. Here, we report the result of this finishing process. The current genome sequence (Build 35) contains 2.85 billion nucleotides interrupted by only 341 gaps. It covers ∼99% of the euchromatic genome and is accurate to an error rate of ∼1 event per 100,000 bases. Many of the remaining euchromatic gaps are associated with segmental duplications and will require focused work with new methods. The near-complete sequence, the first for a vertebrate, greatly improves the precision of biological analyses of the human genome including studies of gene number, birth and death. Notably, the human enome seems to encode only 20,000-25,000 protein-coding genes. The genome sequence reported here should serve as a firm foundation for biomedical research in the decades ahead

Double-trap measurement of the proton magnetic moment at 0.3 parts per billion precision

Precise knowledge of the fundamental properties of the proton is essential for our understanding of atomic structure as well as for precise tests of fundamental symmetries. We report on a direct high-precision measurement of the magnetic moment μ_p of the proton in units of the nuclear magneton μ_N. The result, μ_p = 2.79284734462 (±0.00000000082) μ_N, has a fractional precision of 0.3 parts per billion, improves the previous best measurement by a factor of 11, and is consistent with the currently accepted value. This was achieved with the use of an optimized double–Penning trap technique. Provided a similar measurement of the antiproton magnetic moment can be performed, this result will enable a test of the fundamental symmetry between matter and antimatter in the baryonic sector at the 10⁻¹⁰ level

Progress towards an improved comparison of the proton-to-antiproton charge-to-mass ratios

High-precision comparisons of the proton-to-antiproton charge-to-mass ratios provide sensitive tests of the fundamental charge, parity, time (CPT) invariance. In 2014, we performed such a measurement with a fractional precision of 69 parts in a trillion (p.p.t.). In this article, we describe technical developments which were implemented to improve the precision of our previous measurement by at least a factor of 3

350-fold improved measurement of the antiproton magnetic moment using a multi-trap method

We summarize our recent 1.5 parts per billion measurement of the antiproton magnetic moment using the multi Penning-trap system of the BASE collaboration. The result was achieved by combining the detection of individual spin-transitions of a single antiproton with a novel two-particle spectroscopy technique, which dramatically improved the data sampling rate. This measurement contributes to improve the test of the fundamental charge, parity, time reversal (CPT) invariance in the baryon sector by a factor of 350 compared to our last measurement, and by a factor of 3000 compared to the best competing measurement. We review the measurement technique and discuss the improved limits on CPT-violating physics imposed by this measurement

Antibody-directed evolution reveals a mechanism for enhanced neutralization at the HIV-1 fusion peptide site

Abstract The HIV-1 fusion peptide (FP) represents a promising vaccine target, but global FP sequence diversity among circulating strains has limited anti-FP antibodies to ~60% neutralization breadth. Here we evolve the FP-targeting antibody VRC34.01 in vitro to enhance FP-neutralization using site saturation mutagenesis and yeast display. Successive rounds of directed evolution by iterative selection of antibodies for binding to resistant HIV-1 strains establish a variant, VRC34.01_mm28, as a best-in-class antibody with 10-fold enhanced potency compared to the template antibody and ~80% breadth on a cross-clade 208-strain neutralization panel. Structural analyses demonstrate that the improved paratope expands the FP binding groove to accommodate diverse FP sequences of different lengths while also recognizing the HIV-1 Env backbone. These data reveal critical antibody features for enhanced neutralization breadth and potency against the FP site of vulnerability and accelerate clinical development of broad HIV-1 FP-targeting vaccines and therapeutics