Herpetofauna of a Ramsar Site: The Beeshazar and Associated Lakes, Chitwan National Park, Nepal

The Nepalese herpetofauna has been poorly studied, and little is known about the distribution of the country’s amphibians and reptiles. We surveyed the herpetofauna at Beeshazar and associated lakes, a Ramsar site situated in the buffer zone of Chitwan National Park, Nepal. We conducted surveys between June and July 2015 using line transects, and nocturnal and diurnal visual encounter surveys (VES). We recorded 47 species comprising 13 anurans, 11 lizards, 18 snakes, four turtles, and one crocodilian. This document will serve as source material for outreach activities in conservation awareness of the herpetofauna in the buffer zone of the park. The present study suggests that the species composition of Beeshazar and associated lakes is likely to increase with additional systematic inventories

Economic analysis of fish production using different feed types practiced in Dhanusha district, Nepal

A study on the economic analysis of fish production using different feed types practiced in Dhanusha district was conducted in 2018. Out of 600 fish farmers, sixty fish farmers(10%) from the Fish Superzone region i.e. Janakpur sub-metropolitan city, Bideh municipality, Sahidnagar municipality, Kamala municipality, Hanspur municipality, Janaknandani rural municipality and Aaurahi  rural municipality, selected using simple random sampling, were surveyed using semi-structured questionnaire. The study revealed that locally formulated mash feed was commonly used feed type in which rice bran and mustard oil cake (RB+MOC) was the principle feed ingredient. 55% of the farmers used rice bran and mustard oil cake (RB+MOC), 20% used rice bran, mustard oil cake and soybean (RB+MOC+SOB), 10% used rice bran, mustard oil cake and fish meal (RB+MOC+FM) and 15% farmers mineral and vitamin (RB+MOC+MIN/VIT) in their feed formulation. The productivity of the fish production in feed type RB+MOC+SOB (3.41±1.02) was significantly higher than other feed type.  The total variable cost per ha (5.23±2.11) was found significantly(p<0.05) higher in feed type RB+MOC+SOB. Similarly, gross margin (4.44±2.74) received by farmers of feed type RB+MOC+SOB was also significantly high(p<0.05). The Benefit: Cost (B:C) ratio of the study area was found to be 1.69, high being of feed type RB+MOC+SOB(1.84) compared to others. The feed type RB+MOC+SOB is seen as a economically profitable one in the study area because of the high productivity, profit and B:C ratio. High feed cost was the major problem followed by the unavailability of feed. Fish farming can be a profitable business in Dhanusha with large opportunity to increase the fish production with increasing protein sources in the feed used. Farmers should be provided sufficient information, trainings and be encouraged to incorporate the protein rich sources like soybean and fish meal in the feed prepared to increase the productivity and ultimately return

Fire and forage quality: Postfire regrowth quality and pyric herbivory in subtropical grasslands of Nepal

Fire is rampant throughout subtropical South and Southeast Asian grasslands. However, very little is known about the role of fire and pyric herbivory on the functioning of highly productive subtropical monsoon grasslands lying within the Cwa climatic region. We assessed the temporal effect of fire on postfire regrowth quality and associated pyric-herbivory in the subtropical monsoon grasslands of Bardia National Park, Nepal. Every year, grasslands are burned as a management intervention in the park, especially between March and May. Within a week after fire, at the end of March 2020, we established 60 m x 60 m plots within patches of burned grassland in the core area of the Park. We collected grass samples from the plots and determined physical and chemical properties of the vegetation at regular 30-day intervals from April to July 2020, starting from 30 days after fire to assess postfire regrowth forage quality. We counted pellet groups of cervids that are abundant in the area for the same four months from 2 m x 2 m quadrats that were permanently marked with pegs along the diagonal of each 60 m x 60 m plot to estimate intensity of use by deer to the progression of postfire regrowth. We observed strong and significant reductions in crude protein (mean value 9.1 to 4.1 [55% decrease]) and phosphorus (mean value 0.2 to 0.11 [45% decrease]) in forage collected during different time intervals, that is, from 30 days to 120 days after fire. Deer utilized the burned areas extensively for a short period, that is, up to two months after fire when the burned areas contained short grasses with a higher level of crude protein and phosphorus. The level of use of postfire regrowth by chital (Axis axis) differed significantly over time since fire, with higher intensity of use at 30 days after fire. The level of use of postfire regrowth by swamp deer (Rucervus duvaucelii) did not differ significantly until 90 days after fire, however, decreased significantly after 90 days since fire. Large-scale single event fires, thus, may not fulfil nutritional requirements of all species in the deer assemblage in these subtropical monsoon grasslands. This is likely because the nutritional requirements of herbivores differ due to differences in body size and physiological needs-maintenance, reproduction, and lactation. We recommend a spatiotemporal manipulation of fire to reinforce grazing feedback and to yield forage of high quality for the longest possible period for a sustainable high number of deer to maintain a viable tiger population within the park

Ancient Himalayan wolf (Canis lupus chanco) lineage in Upper Mustang of the Annapurna Conservation Area, Nepal

The taxonomic status of the wolf (Canis lupus) in Nepal’s Trans-Himalaya is poorly understood. Recent genetic studies have revealed the existence of three lineages of wolves in the Indian sub-continent. Of these, the Himalayan wolf, Canis lupus chanco, has been reported to be the most ancient lineage historically distributed within the Nepal Himalaya. These wolves residing in the Trans-Himalayan region have been suggested to be smaller and very different from the European wolf. During October 2011, six fecal samples suspected to have originated from wolves were collected from Upper Mustang in the Annapurna Conservation Area of Nepal. DNA extraction and amplification of the mitochondrial (mt) control region (CR) locus yielded sequences from five out of six samples. One sample matched domestic dog sequences in GenBank, while the remaining four samples were aligned within the monophyletic and ancient Himalayan wolf clade. These four sequences which matched each other, were new and represented a novel Himalayan wolf haplotype. This result confirms that the endangered ancient Himalayan wolf is extant in Nepal. Detailed genomic study covering Nepal’s entire Himalayan landscape is recommended in order to understand their distribution, taxonomy and, genetic relatedness with other wolves potentially sharing the same landscape

Dry season diet composition of four-horned antelope Tetracerus quadricornis in tropical dry deciduous forests, Nepal

It is essential to assess the feeding strategies of threatened species during resource-scarce seasons to understand their dietary niche breadth and inform appropriate habitat management measures. In this study, we examined the diet composition of four-horned antelope (FHA) Tetracerus and quadricornis, one of the least studied ungulate species, in Banke National Park, Nepal. A total of 53 fresh pellet groups were collected between December 2015 and January 2016 and analyzed using micro-histological fecal analysis technique. First, we prepared 133 micro-histological photographs of different parts of 64 reference plant species. Then we compared 1,590 fragments of 53 fecal samples with photographs of reference plants to assess the percentage of occurrence of different plant species in FHA diet. A total of 30 plant species belonging to 18 different families were identified in fecal samples. Chi-square goodness of fit tests showed that FHA appeared not to feed all plant uniformly. Out of 1,520 identified fragments in fecal samples, 1,300 were browse species and 220 were grass species. Browse represented 85.5% of the identified plant fragments, suggesting that FHA might be adopting a browser strategy at least during winter when grasses are low in abundance and their nutritive quality is poor. Tree species had the highest contribution in the diet (46.55%) followed by shrubs (24.52%). The family Gramineae was consumed in the highest proportion (27.68%) followed by Euphorbiaceae (11.95%). Overall, our results suggest that FHA has the feeding plasticity to adapt to resource fluctuation. Based on the findings of this study, we recommend that dicot plant species—particularly fruit trees and shrubs, which are the major source of nutrients for FHA during resource-lean, dry season—be conserved and natural regeneration of these taxa be promoted