Preculturing effect of thidiazuron on in vitro shoot multiplication and micropropagation round in Capparis decidua (Forsk.) an important multipurpose plant

An efficient protocol was developed for clonal multiplication of an important shrub: Capparis decidua (Forsk.) Edgew, through in vitro shoot induction and multiplication from nodal explants. Pretreatment of nodal explants in a liquid Murashige and Skoog (MS) medium augmented with various thidiazuron (TDZ) concentrations at relatively high levels (5–100 μM) for different time duration (4, 8, 12 and 16 d), proved a significant approach for in vitro shoot production. After an initial exposure time to TDZ, nodal explants were inoculated onto a MS basal medium devoid of TDZ for further induction and proliferation. The highest regeneration rate (85%), average number of shoots/explant (8.7 ± 0.22) and maximum shoot length (3.9 ± 0.33 cm) were obtained from the nodal explants exposed to 50 μM TDZ for 8 d. The nodal explants excised from the proliferated cultures of TDZ (50 μM) for 8 d were used as explants and showed an enhancement rate after next three round of in vitro propagation. Best results for rooting was obtained by ex vitro treatment of shoots with 200 μM indole-3-butyric acid (IBA) for 20 min. as it produced an average of 5.7 ± 0.41 roots per microshoot with 4.4 ± 0.39 cm root length in 84% shoots. Different planting substrates was tested for maximum survival of hardening off micropropagated plantlets and soilrite proved most effective than others as 97.1 ± 7.21 plantlets survived. All micropropagated plants grew well in natural conditions and showed similar morphology to the mother plant

Reducing the environmental impact of surgery on a global scale: systematic review and co-prioritization with healthcare workers in 132 countries

Abstract Background Healthcare cannot achieve net-zero carbon without addressing operating theatres. The aim of this study was to prioritize feasible interventions to reduce the environmental impact of operating theatres. Methods This study adopted a four-phase Delphi consensus co-prioritization methodology. In phase 1, a systematic review of published interventions and global consultation of perioperative healthcare professionals were used to longlist interventions. In phase 2, iterative thematic analysis consolidated comparable interventions into a shortlist. In phase 3, the shortlist was co-prioritized based on patient and clinician views on acceptability, feasibility, and safety. In phase 4, ranked lists of interventions were presented by their relevance to high-income countries and low–middle-income countries. Results In phase 1, 43 interventions were identified, which had low uptake in practice according to 3042 professionals globally. In phase 2, a shortlist of 15 intervention domains was generated. In phase 3, interventions were deemed acceptable for more than 90 per cent of patients except for reducing general anaesthesia (84 per cent) and re-sterilization of ‘single-use’ consumables (86 per cent). In phase 4, the top three shortlisted interventions for high-income countries were: introducing recycling; reducing use of anaesthetic gases; and appropriate clinical waste processing. In phase 4, the top three shortlisted interventions for low–middle-income countries were: introducing reusable surgical devices; reducing use of consumables; and reducing the use of general anaesthesia. Conclusion This is a step toward environmentally sustainable operating environments with actionable interventions applicable to both high– and low–middle–income countries

Transfer and Accumulation of Some Heavy Metals in Native Vegetation Plants

Phytoremediation procedure can be defined as the use of selected plants in order to eliminate some heavy metals from the soil, or wastewater in a cost-effective method. This study aimed to investigate the concentrations of heavy metals such as Cd, Pb, Cu, Zn and Cr in soils and vegetation plants grown in Wadi Hanifa, Riyadh city, Kingdom of Saudi Arabia. Five sites have been chosen for collected plant samples (shoot and root) for one year, and five plant species have been chosen which distributed in the study area including Ziziphus spina-christi, Prosopis juliflora, Rhazya stricta, Ochradenus baccatus and Conocarpus erectus. Determination of Cd, Pb, Cu, Zn and Cr has been done with ICP. Accumulation coefficient (AC), and translocation factor (TF) have been calculated to evaluate the ability of selected plants to extract the heavy metals from soil. The results indicated that Ziziphus spina-christi and Conocarpus erectus showed the high ability to accumulate the Pb and Zn in its root and shoot compared with other plants. The trend of heavy metal translocation factors for different plants was in the order of Cd > Cr > Pb > Cu > Zn. The accumulation coefficient (AC) of the Cd, Pb, Zn, Cu and Cr in the roots/soil of Ziziphus spina-christi, Prosopis juliflora, Rhazya stricta, Ochradenus baccatus and Conocarpus erectus were varied from 0.80 to 3.60. The order of AC in the shoot as follows: Pb > Cu > Zn > Cr > Cd, while in roots of as follows: Cd > Cr > Pb > Cr > Zn

Phytochemical and taxonomic evaluation of Rhazya stricta in Saudi Arabia

Rhazya stricta Decne is an important medicinal species used in indigenous medicinal herbal drugs to cure various diseases in South Asia (Pakistan, India and Afghanistan) and in the Middle East (e.g. Saudi Arabia, Qatar, United Arab Emirates (UAE), Iran and Iraq). Some of its alkaloids have been reported to have anticancerous properties. The aim of our study is to examine the morphological and taxonomical parameters for R. stricta in the Saudi Arabia; concentrations and distributions of some secondary metabolites; and also to determine the antioxidant and free radical scavenging activity. The results of present study showed that there was no influence of environment on the structure of stomata and trichomes as studying species with R. stricta. In conclusion our study shows no trichomes on leaf of R. stricta it is glabrous, whereas, variations between many secondary metabolites such as flavonoids and phenolic compounds occurred in response to changing climatic conditions

Field and Modeling Study on Manual and Automatic Irrigation Scheduling under Deficit Irrigation of Greenhouse Cucumber

The primary goal of all those working in the field of sustainable water management, particularly in the arid and semi-arid zones, is to increase irrigation efficiency, reduce irrigation water losses, and improve water productivity for all crops. This study assessed the automatic irrigation scheduling and irrigation management on the growth, yield, and water productivity of cucumber under greenhouse conditions. A field experiment was conducted using cucumber grown in aplastic greenhouse during the winter of 2017/18 and 2018/19 at the research farm station of the National Research Centre (NRC), El-Noubaria Region, Behaira Governorate, Egypt. In a split-plot experiment, two different methods to control irrigation scheduling (manual control (MC) and automatic control (AC)) were used in the main plots and three deficit irrigation treatments (100% of full irrigation (FI), 80% of FI, and 60% of FI). Through the obtained results, it was found that the use of the automatic control of the irrigation schedule led to an improvement in the productivity and quality characteristics of the cucumber crop. Automatic irrigation control created healthy conditions for the plant roots located under the least water stress. This led to an increase in nitrogen uptake at the ages of 3, 5, 7, and 9 weeks after planting in addition to improving the total leaf area and the chlorophyll content of leaves, which consequently had a greater effect on increasing yield and water productivity of cucumber. Although the highest values of cucumber productivity were obtained with irrigation at 100% of FI, there were no significant differences between 100% FI and 80% of FI, therefore it is preferable to irrigate at 80% of FI, and this means saving 20% of irrigation water that can be used to irrigate other areas. The SALTMED model simulating all of the following evaluation criteria performed well for soil moisture content and N-uptake as well as the leaves area, the yield, and water productivity of cucumber for all treatments for the two growing seasons 2017/18 and 2018/19, with the overall R2 of 0.882, 0.903, 0.975, 0.907, and 0.933, respectively

Grasses in Semi-Arid Lowlands—Community Composition and Spatial Dynamics with Special Regard to the Influence of Edaphic Factors

Edaphic properties have been widely shown to influence community composition and distribution. However, the degree to which edaphic factors can affect grasses in semi-arid lowlands is still little researched. We assessed the significance of nine edaphic factors to explain the distributions of 65 grass species with various ecological traits (i.e., the ecological indicator values for their preferred habitat) in the semi-arid lowlands of Pakistan. To record information on species composition and related ecological conditions, we selected 10 random sampling locations between 2020 and 2021. For each species, we determined the important value index (IVI) and looked at the primary indicator species that were identified using the indicator species analysis approach. The major genera were Setaria, Brachiaria, and Cenchrus with 6.15% species in each followed by Aristida, Panicum, and Eragrostis with 4.61% wild grass species, Bothriochloa, Bromus, Phragmites, Polypogon, Saccharum, Poa, Echinochloa, and Dactyloctenium with 3.07% species, whereas other genera had a single species each. In total, 80% of the species were native, while only 20% were introduced species. Microphylls accounted for 49.23% of the leaf size spectra of the grass flora in the study area. The other frequent traits included macrophylls (21.53%), nanophylls (20%), and leptophylls (9.23%). The major life forms were therophytes (56.92%) followed by hemicryptophytes (38.46%) and geophytes (4.61%). The results of an ordination analysis indicated that the distribution of grasses was significantly (p ≤ 0.002) influenced by several edaphic parameters, with pH having the greatest impact on species distribution. The analyses of indicator species showed that pH and EC were the most powerful and important edaphic factors for determining the composition of plant communities and indicator species. The significant indicator species in various ecosystems were Cynodon dactylon (L.) Pers. [email protected] and Leptochloa chinensis (L.) Nees (agroecosystem), Brachiaria reptans (L.) C.A. Gardner and C.E. Hubb, Dichanthium annulatum (Forssk.) Stapf, and Saccharum spontaneum L. (forest ecosystem), Cenchrus biflorus Roxb., Cenchrus ciliaris L., and Desmostachya bipinnata (L.) Stapf (urban ecosystem), Arundo donax L., Echinochloa crus-galli, and Phragmites australis (wetland ecosystem), and Saccharum spontaneum and Echinochloa crus-galli (L.) P. Beauv. (riparian ecosystem). We discovered that different species groupings had different habitat preferences and that soil pH had a significant beneficial effect on plant variety. These results provide a scientific roadmap for soil and plant restoration in semi-arid lowland habitats

Principal Component and Path Analysis for Trait Selection Based on the Assessment of Diverse Lentil Populations Developed by Gamma-Irradiated Physical Mutation

Publisher Copyright: Copyright © 2022 Sandip Debnath et al.Lentil is a notable legume crop valued for its high protein, vitamin, mineral, and amino acid (lysine and tryptophan) content. This crop has a narrow genetic base due to the formation of gene pool barriers during interspecific hybridization within and across species. Mutagenesis may be seen as a novel and alternative breeding technique for the production of new diversity. For the identification of new alleles, the creation of mutants followed by selection in subsequent generations would be necessary. Induction of mutation in lentil cv. Moitree by gamma rays therefore produced high variation for the majority of quantitative measures examined. Henceforth, principal component analysis (PCA) and path coefficient analysis were conducted to identify and exclude redundant mutant genotypes with similar traits as the success of breeding is dependent on understanding the relationship between morpho-agronomic traits and seed yield. As shown by the findings of this research, the total quantity of pods per mutant plant should be given considerable priority. The identified mutant genotypes, such as lines 24, 43, 28, 33, and 10, may be used as parents in future breeding or released directly following trials.Peer reviewe