Design and Implementation of Network Public Opinion Analysis System

Network public opinion analysis is an important way of information analysis processing. This paper based on the research of the related technologies, designs and realizes a new network public opinion analysis system. System mainly includes network data fetching part, fetching the data processing part, analyzes the processed data part and display part of the public opinion analysis results. In the document extraction part, used the web crawler technology, Larbin web crawler to realize the collection of web content; In public opinion information analysis part, the implementation of the new topic adopts an improved Single - Pass clustering algorithm. This algorithm is using of multi-center, using the title and body of the vector to compared two-way ,that is better reflect the dynamics of public opinion topics. Finally, in the network environment of a university, we have the tests repeatedly. The results show that the new public opinion analysis system running is stable and has good efficiency. The thesis has certain value for the development of other information analysis systems in the Internet

A process systems Engineering approach to analysis of fructose consumption in the liver system and consequences for Non-Alcoholic fatty liver disease

Metabolic disturbances to the liver system can induce lipid deposition and subsequently cause non-alcoholic fatty liver disease (NAFLD). Increasing consumption of fructose has been proposed as a crucial risk component in the development of NAFLD. Three potential therapeutic targets in the network were explored using a composite model of liver function. Introducing a fructose enriched diet under insulin resistance conditions was simulated to evaluate the effectiveness of the model in novel therapy design. In vitro experiments were conducted on rat liver samples to assess the robustness of the model predictions. Synergistic application of all three interventional points in silico has been predicted as the most effective treatment to reduce lipid production under both moderate and severe insulin resistance conditions. This study demonstrates how we can use system models together with in vivo experiments to explore the behaviour of the liver system in response to fructose variation and use it to help identify possible drug targets

_In vivo_ photoacoustic molecular imaging with simultaneous multiple selective targeting using antibody-conjugated gold nanorods

The use of gold nanorods for photoacoustic molecular imaging in vivo with simultaneous multiple selective targeting is reported. The extravasation of multiple molecular probes is demonstrated, and used to probe molecular information of cancer cells. This technique allows molecular profiles representing tumor characteristics to be obtained and a heterogeneous population of cancer cells in a lesion to be determined. The results also show that the image contrast can be enhanced by using a mixture of different molecular probes. In this study, HER2, EGFR, and CXCR4 were chosen as the primary target molecules for examining two types of cancer cells, OECM1 and Cal27. OECM1 cells overexpressed HER2 but exhibited a low expression of EGFR, while Cal27 cells showed the opposite expression profile. Single and double targeting resulted in signal enhancements of up to 3 dB and up to 5 dB, respectively, and hence has potential in improving cancer diagnoses

Multiple tumor suppressors regulate a HIF-dependent negative feedback loop via ISGF3 in human clear cell renal cancer.

Whereas VHL inactivation is a primary event in clear cell renal cell carcinoma (ccRCC), the precise mechanism(s) of how this interacts with the secondary mutations in tumor suppressor genes, including PBRM1, KDM5C/JARID1C, SETD2, and/or BAP1, remains unclear. Gene expression analyses reveal that VHL, PBRM1, or KDM5C share a common regulation of interferon response expression signature. Loss of HIF2α, PBRM1, or KDM5C in VHL-/-cells reduces the expression of interferon stimulated gene factor 3 (ISGF3), a transcription factor that regulates the interferon signature. Moreover, loss of SETD2 or BAP1 also reduces the ISGF3 level. Finally, ISGF3 is strongly tumor-suppressive in a xenograft model as its loss significantly enhances tumor growth. Conversely, reactivation of ISGF3 retards tumor growth by PBRM1-deficient ccRCC cells. Thus after VHL inactivation, HIF induces ISGF3, which is reversed by the loss of secondary tumor suppressors, suggesting that this is a key negative feedback loop in ccRCC. © 2018, Liao et al

The Effectiveness of Tepid Sponge Bath with 32oc and 37oc to Decrease Body Temperature at Toddler with Fever

Introduction: Tepid sponge bath is a therapeutic bath by washing all around of the body with warm water to decrease body temperature. Warm water that used were 32oC (nail warm) and 37oC (warm). The aimed of this study was to compare the effectivity of tepid sponge bath with 32oC and 37oC warm water on decreasing body temperature at toddler with fever. Method: A quasy experimental pre post test design was used in this study. The population was toddler who had body temperature ≥38oC which treated in anggrek pediatric room dr. Iskak public hospital Tulungagung. There were 26 respondents recruited by using purposive sampling technique and divided into two group, each of 13 respondents received tepid sponge bath with 32oC and others received tepid sponge bath with 37oC warm water. The independent variable was tepid sponge bath and dependent variable was body temperature. Data were collected by using digital termometere and noted in respondent observation, then analyzed by using Paired t-Test and Mann Withney U-Test. Result: The result showed that there was an effectivity on decreasing body temperature by giving tepid sponge bath with 32oC and 37oC warm water with significance level p=0.000 and there was a difference decreasing body temperature among both of them with significance level p=0.016. Discussion: It can be concluded that tepid sponge bath with 37oC warm water was more effective than tepid sponge bath with 32oC warm water. Further studies should be observed the effectivity of tepid sponge bath with more specific age, fever character and more time and respondent

A novel anti-virulence gene revealed by proteomic analysis in Shigella flexneri 2a

<p>Abstract</p> <p>Background</p> <p><it>Shigella flexneri </it>is a gram-negative, facultative pathogen that causes the majority of communicable bacterial dysenteries in developing countries. The virulence factors of <it>S. flexneri </it>have been shown to be produced at 37 degrees C but not at 30 degrees C. To discover potential, novel virulence-related proteins of <it>S. flexneri</it>, we performed differential in-gel electrophoresis (DIGE) analysis to measure changes in the expression profile that are induced by a temperature increase.</p> <p>Results</p> <p>The ArgT protein was dramatically down-regulated at 37 degrees C. In contrast, the ArgT from the non-pathogenic <it>E. coli </it>did not show this differential expression as in <it>S. flexneri</it>, which suggested that <it>argT </it>might be a potential anti-virulence gene. Competitive invasion assays in HeLa cells and in BALB/c mice with <it>argT </it>mutants were performed, and the results indicated that the over-expression of ArgT_Y225Dwould attenuate the virulence of <it>S. flexneri</it>. A comparative proteomic analysis was subsequently performed to investigate the effects of ArgT in <it>S. flexneri </it>at the molecular level. We show that HtrA is differentially expressed among different derivative strains.</p> <p>Conclusion</p> <p>Gene <it>argT </it>is a novel anti-virulence gene that may interfere with the virulence of <it>S. flexneri </it>via the transport of specific amino acids or by affecting the expression of the virulence factor, HtrA.</p

The Long-term Monitoring Results of Insight-HXMT in the First 4 Yr Galactic Plane Scanning Survey

The first X-ray source catalog of Insight-HXMT Galactic Plane (|b|<10deg) Scanning Survey (GPSS) is presented based on the data accumulated from June 2017 to August 2021. The 4 yr limit sensitivities at main energy bands can reach 8.2x10^(-12) erg/s/cm^2} (2-6 keV), 4.21x10^(-11) erg/s/cm^2 (7-40 keV) and 2.78x10^(-11) erg/s/cm^2 (25-100 keV). More than 1300 sources have been monitored at a wide band (1$-$100\,keV), of which 223 sources have a signal-to-noise ratio greater than 5. We combined the GPSS data of Insight-HXMT and MAXI and found it is feasible to obtain more complete long-term light curves from their scanning results. The flux variabilities at different energy bands of the 223 bright sources are analyzed based on the excess variances. It is found that the fluxes of X-ray binaries are more active than those of supernova remnants and isolated pulsars. Different types of binaries, e.g., low-mass X-ray binaries (LMXBs), high-mass X-ray binaries (HMXBs), neutron star binaries, and black hole binaries, also distinctively show different regularities. In addition, the relations between the hardness ratio (HR) and excess variances, and HR and source types are analyzed. It is obvious that the HRs of HMXBs tend to be harder than those of LMXBs and HMXBs tend to be more active than those of LMXBs.Comment: 43 pages, 26 figures, accepted for publication in ApJ

Practice on the Watershed Hydrological Experimental System Reconciling Deterministic and Stochastic Subjects Based on the System Complexity: 1. Theoretical Study

This is the first of a two-part series on the watershed hydrological experimental system (WHES). Since the foundational stage and developmental stage of hydrological basin study with a duration of more than ca. one century, facing with the changing environment and, the declined risk of field study while the catchment hydrology is trapped in a theoretical impasse, a third phase of renovation on hydrological experiments seems ready to come out inevitably. Learned from Chinese decades’ experiences on the field basin study for the question of what is wrong with the status quo, our exploratory idea is reported in this part. From the viewpoint of general system theory based on the paralleled concepts of the ancient Chinese and the Western, it is considered that the adequate method should face the characters of the complex dynamic system instead of previous static, linear system. From the viewpoint of another philosophical paralleled concept of the Middle Way, it should also face the operation and organizing of the mesoscopic systems for the organized complexity. Then, a framework of WHES is suggested with its organization based on the strategy of constrain complexity and add complexity and on the strategy of manipulation including the artificial-natural and controlled-natural objects. Such a trial framework, the Chuzhou WHES, is reported including the suggested critical zone experimental block (CZEB) instead of the experimental basin (EB) in the last decades