8,635 research outputs found
Heritability and fitness-related consequences of squid personality traits
Dumpling squid, Euprymna tasmanica, show consistent individual differences in behaviour that can be classified according to indices reflecting shy–bold, activity and reactivity responses. Using crosses of wild-caught single males to multiple females with known behavioural phenotypes, this study estimated patterns of additive genetic and residual variance in these behavioural traits from offspring of squid in two contexts, a threat (antipredator) and feeding (foraging) test. Genetic contributions to behavioural expression were dependent on test context. Behaviours in antipredator contexts had significant heritabilities (h2 = 0.2–0.8) while behaviours from foraging contexts had lesser additive genetic and greater residual components (h2 = 0.05–0.08). Personality trait variation in females was not related to her fecundity. Female boldness in foraging situations, which co-varied with body size, explained small but significant variation (∼21%) in brood hatching success, while successful fertilization was determined by positive assortion of mate pairs according to their shy–bold phenotype. These results are discussed in terms of the ecological and evolutionary significance of animal 'personality' traits in wild populations of animals
Long-term changes in soil microbial communities during primary succession
Soil microbial communities (SMCs) play a critical role in the cycling of carbon and nutrients in
terrestrial ecosystems, as well as regulating plant productivity and diversity. However, very
little is known about long-term (decades-centuries) structural changes in these communities.
The development of aboveground-belowground linkages during century-scale succession is
also poorly understood. Our study addressed this knowledge gap by investigating SMC and
plant communities undergoing primary succession on an 850-year chronosequence of lava
flows in Iceland. We hypothesised that communities of microfungi and bacteria would
respond to progressive changes in vegetation and that SMC diversity would increase with
terrain age. Soil samples were collected from three lava flows at different stages of primary
succession (165, 621 and 852 years after lava flow emplacement). Plant community
composition was surveyed as the samples were collected. The composition of the SMCs
present in the soil was determined using amplicon pyrosequencing. The physical and
chemical properties of the soil were also analysed. The results of the study indicated
changes in plant and fungal communities with increasing terrain age. Distinct plant and
fungal assemblages were identified on the three sites and both communities became richer
and more diverse with increasing terrain age. There was also evidence to suggest the
development of mycorrhizal associations on older sites. In contrast, the composition and
structure of the bacterial communities did not change systematically with terrain age.
Similarly, there were few changes in soil properties: SOM concentrations and pH, both of
which have been demonstrated to be important to SMCs, were constant across the
chronosequence. These results suggest that plant community composition is significant for
fungal communities, but less relevant for bacterial communities. This finding has implications
for studies of primary succession and the biogeochemical impact of vegetation change in
high-latitude ecosystems.This work was funded by a
Small Ecological Project Grant from the British Ecological Society (grant number 2812/3507)
and grants from the John Fell Fund, University of Oxford (grant number 091/148) and Trinity
College, University of Cambridge.This is the accepted manuscript. The final version is available at http://www.sciencedirect.com/science/article/pii/S003807171300429X
Systemic inflammatory response exacerbates the neuropsychological effects of induced hyperammonemia in cirrhosis
Background/Aims: Studies in acute liver failure show correlation between evidence of a systemic inflammatory response syndrome (SIRS) and progression of hepatic encephalopathy (HE). We tested the hypothesis that SIRS mediators, such as nitric oxide and proinflammatory cytokines, may exacerbate the neuropsychological effects of hyperammonemia in cirrhosis.Methods: Ten patients with cirrhosis were studied, 24-36 h after admission with clinical evidence of infection, and following its resolution. Hyperammonemia was induced by oral administration of an amino-acid (aa) solution mimicking hemoglobin composition. Inflammatory mediators, nitrate/nitrite, ammonia, aa profiles and a battery of neuropsychological tests were measured.Results: The hyperammonemia generated in response to the aa solution was similar prior to, and after resolution, of the inflammation (P = 0.77). With treatment of the infection there were significant reductions in white blood cell count (WBC), C-reactive protein (CRP), nitrate/nitrite, interleukin-6, interieukin-1beta and tumour necrosis factor alpha. Induced hyperammonemia resulted in significant worsening of the neuropsychological scores when patients showed evidence of SIRS but not after its resolution.Conclusions: The significant deterioration of neuropsychological test scores following induced hyperammonemia during the inflammatory state, but not after its resolution, suggests that the inflammation and its mediators may be important in modulating the cerebral effect of ammonia in liver disease. (C) 2003 European Association for the Study of the Liver. Published by Elsevier B.V. All rights reserved
GLP-1 action in the mouse bed nucleus of the stria terminalis
Glucagon-like peptide-1 (GLP-1) injected into the brain reduces food intake. Similarly, activation of preproglucagon (PPG) cells in the hindbrain which synthesize GLP-1, reduces food intake. However, it is far from clear whether this happens because of satiety, nausea, reduced reward, or even stress. Here we explore the role of the bed nucleus of the stria terminalis (BNST), an area involved in feeding control as well as stress responses, in GLP-1 responses. Using cre-expressing mice we visualized projections of NTS PPG neurons and GLP-1R-expressing BNST cells with AAV-driven Channelrhodopsin-YFP expression. The BNST displayed many varicose YFP+ PPG axons in the ventral and less in the dorsal regions. Mice which express RFP in GLP-1R neurons had RFP+ cells throughout the BNST with the highest density in the dorsal part, suggesting that PPG neuron-derived GLP-1 acts in the BNST. Indeed, injection of GLP-1 into the BNST reduced chow intake during the dark phase, whereas injection of the GLP-1 receptor antagonist Ex9 increased feeding. BNST-specific GLP-1-induced food suppression was less effective in mice on high fat (HF, 60%) diet, and Ex9 had no effect. Restraint stress-induced hypophagia was attenuated by BNST Ex9 treatment, further supporting a role for endogenous brain GLP-1. Finally, whole-cell patch clamp recordings of RFP+ BNST neurons demonstrated that GLP-1 elicited either a depolarizing or hyperpolarizing reversible response that was of opposite polarity to that under dopamine. Our data support a physiological role for BNST GLP-1R in feeding, and suggest complex cellular responses to GLP-1 in this nucleus
Methods for measuring fluoroscopic skin dose
This paper briefly reviews available technologies for measuring or estimating patient skin dose in the interventional fluoroscopic environment
Синтез нечетких систем автоматического управления генетическими алгоритмами по векторным критериям в среде MATLAB
Задачи многокритериального параметрического синтеза систем управления сведены к задачам оптимизации векторных целевых функций, решение которых позволяет удержать процесс синтеза систем в допустимой области. Для оптимизации векторных целевых функций систем автоматического управления модифицированы бинарный и непрерывный генетические алгоритмы. Показана эффективность применения модифицированных генетических алгоритмов для синтеза систем управления путем оптимизации векторных целевых функций. Рассмотрение задач синтеза линейных и нечетких ПИД регуляторов показало, что в задаче синтеза нечеткого регулятора определяется вектор переменных параметров большей размерности, а в модели системы управления вместо линейных уравнений применяются нелинейные уравнения с использованием системы нечеткого вывода
Characterization of the Prophage Repertoire of African Salmonella Typhimurium ST313 Reveals High Levels of Spontaneous Induction of Novel Phage BTP1
In the past 30 years,Salmonella bloodstream infections have become a significant health problem in sub-Saharan Africa and are responsible for the deaths of anestimated 390,000 people each year. The disease is predominantly caused by a recently described sequence type of SalmonellaTyphimurium: ST313, which has a distinctive set of prophage sequences. We have thoroughly characterized the ST313-associated prophages both genetically and experimentally. ST313 representative strain D23580 contains five full-length prophages: BTP1, Gifsy-2D23580, ST64BD23580, Gifsy-1D23580,and BTP5. We show that commonS.Typhimurium prophages Gifsy-2, Gifsy-1, andST64B are inactivated in ST313 by mutations. Prophage BTP1 was found to be a functional novel phage, and the first isolate of the proposed new species “Salmonellavirus BTP1”, belonging to the P22virusgenus. Surprisingly,∼109BTP1 virus particlesperml were detected in the supernatant of non-induced, stationary-phase culturesof strain D23580, representing the highest spontaneously induced phage titer so farreported for a bacterial prophage. High spontaneous induction is shown to be anintrinsic property of prophage BTP1, and indicates the phage-mediated lysis of around0.2% of the lysogenic population. The fact that BTP1 is highly conserved in ST313 poses interesting questions about the potential fitness costs and benefits of novel prophagesin epidemicS.Typhimurium ST313
Visible light responsive titanium dioxide (TiO<inf>2</inf>)
Titanium dioxide (TiO2) is one of the most researched semiconductor oxides that has revolutionised technologies in the field of environmental purification and energy generation. It has found extensive applications in heterogenous photocatalysis for removing organic pollutants from air and water and also in hydrogen production from photocatalytic water-splitting. Its use is popular because of its low cost, low toxicity, high chemical and thermal stability, But one of the critical limitations of TiO 2 as photocatalyst is its poor response to visible light. Several attempts have been made to modify the surface and electronic structures of TiO2 to enhance its activity in the visible light region such as noble metal deposition, metal ion loading, cationic and anionic doping and sensitisation, Most of the results improved photocatalytic performance under visible light irradiation. This paper attempts to review and update some of the information on the TiO2 photocatalytic technology and its accomplishment towards visible light region
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