566 research outputs found

    A Framework for Efficient Adaptively Secure Composable Oblivious Transfer in the ROM

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    Oblivious Transfer (OT) is a fundamental cryptographic protocol that finds a number of applications, in particular, as an essential building block for two-party and multi-party computation. We construct a round-optimal (2 rounds) universally composable (UC) protocol for oblivious transfer secure against active adaptive adversaries from any OW-CPA secure public-key encryption scheme with certain properties in the random oracle model (ROM). In terms of computation, our protocol only requires the generation of a public/secret-key pair, two encryption operations and one decryption operation, apart from a few calls to the random oracle. In~terms of communication, our protocol only requires the transfer of one public-key, two ciphertexts, and three binary strings of roughly the same size as the message. Next, we show how to instantiate our construction under the low noise LPN, McEliece, QC-MDPC, LWE, and CDH assumptions. Our instantiations based on the low noise LPN, McEliece, and QC-MDPC assumptions are the first UC-secure OT protocols based on coding assumptions to achieve: 1) adaptive security, 2) optimal round complexity, 3) low communication and computational complexities. Previous results in this setting only achieved static security and used costly cut-and-choose techniques.Our instantiation based on CDH achieves adaptive security at the small cost of communicating only two more group elements as compared to the gap-DH based Simplest OT protocol of Chou and Orlandi (Latincrypt 15), which only achieves static security in the ROM

    Structural elucidation of natural 2-hydroxy di- and tricarboxilic acids and esters, phenylpropanoid esters, and flavonoids extracted from the bulbs of Autonoë madeirensis using GC-EIMS, ESIMS and MS/MS techniques

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    Comunicação oral sob a forma de PosterAutonoë madeirensis (Menezes) Speta is a Hyacinthaceae endemic from the Portuguese Archipelago of Madeira.1,2 Previous studies on Hyacinthaceae species have shown that they produce a large number of biologically important secondary metabolites, such as triterpenoid and steroid glycosides3, flavone and homoisoflavanone glycosides4 and polyhydroxyalkaloids.5 Included in a systematic phytochemical study of the bulbs of A. madeirensis, we report now the structural identification of three different classes of compounds, mainly by GC-EIMS, ESIMS and tandem mass spectrometry. The phytochemical study was performed using total ethanolic extracts that were fractionated by solvents of increasing polarity. The compounds identified were 2-hydroxy di- and tricarboxilic acids and esters (malic acid, ethylmethylmalate, diethylmalate, citric acid, monomethylcitrate, monoethylcitrate and triethylcitrate), hydroxycinnamic esters (methyl p-coumarate, ethyl p-coumarate, and methyl ferulate), and the three major anthocyanins (cyanidin coumaroyl glycoside, peonidin coumaroyl glycoside and delphinidin diacetyl diglycoside) and a flavone diglucoside, being the interglucosidic linkage (12), to the best of our knowledge, reported for the first time in a diglucoside of apigenin. The analytic conditions, retention times and fragmentation pattern reported now may constitute a fast tool for the systematic identification of these compounds in plant extracts. References: 1- Speta, F., 1998. Systematishe Analyse de gattung Scilla L. s.l. (Hyacinthaceae). Phyton 38(1), 87-95, 121-123. 2- Speta, F., 1998. Hyacinthaceae. In: Kubitzki, K., (Ed.). The families and genera of vascular plants. III. Flowering plants: Monocotyledons. Springer Verlag, Berlin, pp. 261-285. 3- Kopp, B., Krenn, L., Draxler, M., Hoyer, A., Terkola, R., Vallaster, P., Robien, W., 1996. Bufadienolides from Urginea maritima from Egypt. Phytochemistry 42(2), 513-522. 4- Harborne, J.B., Williams, C.A., 1994. Recent advances in the chemosystematics of the monocotyledons. Phytochemistry 37(1), 3-18. 5- Kite, G.C., Grayer, R., Rudall, P., Simmonds, M.S.J., 2000. The potential for chemical characters in monocotyledon systematics. in: Wilson, K.L., Morrison, D.A., (Eds.). Monocotyledons: systematics and evolution. CSIRO, Melbourne, pp. 101-113.This research was carried out with financial support from Fundação para a Ciência e Tecnologia (FCT/POCTI/FEDER) and Project POCTI QUI/119/94 “Structure and Reactivity of Flavonoid Glycosides and Synthetic Analogues: A Fundamental Research by Mass Spectrometry”

    Hidrogenionic potential (pH) of the attractant, trap density and control threshold for Ceratitis capitata (Diptera: tephritidae) on Hamlin oranges in São Paulo central region, Brazil

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    This study evaluated the effect of initial pH values of 4.5, 6.5 and 8.5 of the attractant (protein bait) Milhocina® and borax (sodium borate) in the feld, on the capture of fruit flies in McPhail traps, using 1, 2, 4 and 8 traps per hectare, in order to estimate control thresholds in a Hamlin orange grove in the central region of the state of São Paulo. The most abundant fruit fly species was Ceratitis capitata, comprising almost 99% of the fruit flies captured, of which 80% were females. The largest captures of C. capitata were found in traps baited with Milhocina® and borax at pH 8.5. Captures per trap for the four densities were similar, indicating that the population can be estimated with one trap per hectare in areas with high populations. It was found positive relationships between captures of C. capitata and the number of Hamlin oranges damaged, 2 and 3 weeks after capture. It was obtained equations that correlate captures and damage levels which can be used to estimate control thresholds. The average loss caused in Hamlin orange fruits by C. capitata was 2.5 tons per hectare or 7.5% of production.Esta pesquisa teve como objetivos: avaliar o efeito do pH inicial, 4.5; 6.5 e 8.5, do atrativo proteico Milhocina® e bórax (tetraborato de sódio) na captura de moscas-das-frutas em armadilhas McPhail; estudar densidades de armadilhas, 1; 2; 4 e 8 por hectare, para estimar níveis de controle em laranja cv. Hamlin, na região central de São Paulo. A espécie predominante, com 99% das moscas-das-frutas capturadas, foi Ceratitis capitata, sendo 80% de fêmeas. As maiores capturas de C. capitata ocorreram nas armadilhas com Milhocina® e bórax em pH 8.5. As capturas, nas 4 densidades, foram semelhantes, indicando que a população pode ser estimada com uma armadilha por hectare em áreas de altas populações. Houve relações positivas entre capturas de C. capitata e o número de frutos danificados, 2 e 3 semanas após a captura. Assim, foram obtidas equações que relacionam a captura e o dano, possibilitando estimar níveis de controle desse inseto. As perdas médias causadas por C. capitata em laranja cv. Hamlin chegaram a 2,5 toneladas de frutos por hectare ou 7,5% da produção.info:eu-repo/semantics/publishedVersio

    An alternative method for screening lactic acid bacteria for the production of exopolysaccharides with rapid confirmation.

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    Abstract: The accumulation of exopolysaccharides (EPS) produced by microorganisms occurs in the presence of excess substrate and limiting conditions of elements that are essential to growth, such as nitrogen, phosphorus, sulfur, and magnesium. The presence of EPS produced by bacterial cells contributes to slime colonies formation in solid medium and increased viscosity in liquid medium. This paper proposes an alternative method for screening EPS-producing lactic acid bacteria using solid medium-containing discs of filter paper that are saturated with active cultures. The screening was carried out under different culture conditions varying the type of sugar, pH, and temperature. EPS production was visualized by the presence of mucoid colonies on the discs, which was confirmed by the formation of a precipitate when part of this colony was mixed with absolute alcohol. The established conditions for obtaining a high number of isolates producing EPS were 10% sucrose, pH 7.5 and 28 °C. This method proved to be effective and economical because several strains could be tested on the same plate, with immediate confirmation. Resumo: Em geral, o acúmulo de exopolissacarídeos (EPS) produzidos pelos microrganismos ocorre na presença de excesso de substrato e condições limitantes de elementos essenciais ao crescimento, como nitrogênio, fósforo, enxofre, ou magnésio. A presença de EPS associada com células bacterianas é reconhecida em meio sólido para a formação de colônias mucoides e no meio líquido pelo aumento da viscosidade. Este trabalho propõe um método alternativo para realizar triagem de bactérias láticas produtoras de EPS utilizando meio sólido contendo discos de papel de filtro impregnado pela cultura. A seleção foi realizada em diferentes condições de cultivo, variando o tipo de açúcar, pH e temperatura. A produção de EPS foi visualizada pela presença da colônia mucoide sobre os discos, que foram confirmadas pelo precipitado, quando parte desta colônia foi misturada com álcool absoluto. As condições de cultivo em que houve maior produção de EPS foram: sacarose 10%, pH 7,5 a 28 °C. Este método provou ser eficaz e econômico, uma vez que várias cepas podem ser testadas na mesma placa, sendo possível realizar uma prévia confirmação da produção do EPS

    Apreciação dos jogos por crianças e jovens em um festival de handebol

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    Nosso interesse pelo campo teórico da Praxiologia Motriz teve início no projeto de extensão universitária denominado “Ensinando e Aprendendo Handebol”, espaço no qual nos deparamos, já há quase 10 anos, com o desafio de ensinar handebol à crianças e jovens. Associados a este projeto de extensão, sentimos a necessidade de um grupo de pesquisa, no qual pudéssemos nos aprofundar nos estudos acerca da pedagogia dos esportes coletivos, surgindo, a partir do ano de 2009, o Núcleo de Estudos e Pesquisas das Abordagens Táticas dos Esportes Coletivos (NEPATEC).Departamento de Educación Físic

    Evaluation and molecular characterization of human adenovirus in drinking water supplies: viral integrity and viability assays

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    BACKGROUND: Human adenoviruses (HAdVs) are the second-leading cause of childhood gastroenteritis worldwide. This virus is commonly found in environmental waters and is very resistant to water disinfection and environmental stressors, especially UV light inactivation. Molecular techniques, such as PCR-based methods (Polymerase Chain Reaction), are commonly used to detect and identify viral contamination in water, although PCR alone does not allow the discrimination between infectious and non-infectious viral particles. A combination of cell culture and PCR has allowed detection of infectious viruses that grow slowly or fail to produce cytopathic effects (CPE) in cell culture. This study aimed to assess the integrity and viability of human adenovirus (HAdV) in environmental water and evaluate circulating strains by molecular characterization in three sites of the water supply in Florianópolis, Santa Catarina Island, Brazil: Peri Lagoon water, spring source water, and water from the public water supply system. METHODS: Water samples were collected, concentrated and HAdV quantified by real-time PCR. Viral integrity was evaluated by enzymatic assay (DNase I) and infectivity by plaque assay (PA) and integrated cell culture using transcribed mRNA (ICC-RT-qPCR). Samples containing particles of infectious HAdV were selected for sequencing and molecular characterization. RESULTS: The analyzed sites contained 83, 66 and 58% undamaged HAdV particles (defined as those in which the genetic material is protected by the viral capsid) at Peri Lagoon, spring source water and public supply system water, respectively. Of these, 66% of the particles (by PA) and 75% (by ICC-RT-qPCR) HAdV were shown to be infectious, due to being undamaged in Peri Lagoon, 33% (by PA) and 58% (by ICC-RT-qPCR) in spring source water and 8% (by PA) and 25% (by ICC-RT-qPCR) in the public water supply system. ICC-RT-qPCR, a very sensitive and rapid technique, was able to detect as low as 1 × 10(2) HAdV genome copies per milliliter of infectious viral particles in the environmental water samples. The molecular characterization studies indicated that HAdV-2 was the prevalent serotype. CONCLUSIONS: These results indicate a lack of proper public health measures. We suggest that HAdV can be efficiently used as a marker of environmental and drinking water contamination and ICC-RT-qPCR demonstrated greater sensitivity and speed of detection of infectious viral particles compared to PA

    Microstructure identification via detrended fluctuation analysis of ultrasound signals

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    We describe an algorithm for simulating ultrasound propagation in random one-dimensional media, mimicking different microstructures by choosing physical properties such as domain sizes and mass densities from probability distributions. By combining a detrended fluctuation analysis (DFA) of the simulated ultrasound signals with tools from the pattern-recognition literature, we build a Gaussian classifier which is able to associate each ultrasound signal with its corresponding microstructure with a very high success rate. Furthermore, we also show that DFA data can be used to train a multilayer perceptron which estimates numerical values of physical properties associated with distinct microstructures.Comment: Submitted to Phys. Rev.

    Modeling Architectures and Reference Models: Development and Maintenance Open Source ERP

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    The adoption Enterprise Resource Planning (ERPs) by small and medium-sized businesses may not possible its cost. At same time, whenever adapt ERP specific needs company, user becomes dependent developers due to the lack access and knowledge respective code. Free and open source software can promote advantages companies, however, for their adoption, it is necessary to develop techniques tools facilitate implementation and maintenance code. This article highlights the importance of defining modeling architectures and reference models for development and maintenance open source ERPs, especially the ERP5 project

    Miocardiopatia dilatada: Um relato de caso / Dilated cardiomyopathy: A case report

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    Introdução: A Miocardiopatia Dilatada é o subtipo mais comum de um grupo de doenças em que a disfunção primária se encontra no miocárdio. Caracteriza-se por dilatação ventricular esquerda, principalmente, associado a déficit contrátil e adelgaçamento de suas paredes. É responsável por mais de 30% dos casos de Insuficiência Cardíaca na população; e um diagnóstico precoce e início de tratamento adequado influenciam na sua morbimortalidade. Objetivos: Relatar o caso de um paciente diagnosticado com miocardiopatia dilatada e discutir sobre os métodos diagnósticos, possíveis etiologias e as dificuldades para defini-la. Métodos: Foi realizada uma coleta de informações em prontuário do paciente, seguida de entrevista e análise de exames realizados. Cerca de 24 artigos foram selecionados utilizando as plataformas do PubMed, UptoDate e Lilacs, além de 3 livros-texto e 1 diretriz. Relato de Caso: Paciente do sexo masculino, 35 anos, frequentador da APAE, há 6 anos iniciou quadro de cansaço, dispneia aos grandes esforços e tosse noturna. Após atendimento ambulatorial, foi evidenciado uma cardiomegalia à radiografia, iniciado tratamento clínico e encaminhado a Clínica de Insuficiência Cardíaca. Ao ecocardiograma, foi diagnosticado com miocardiopatia dilatada, com múltiplos sintomas de insuficiência cardíaca com baixa fração de ejeção, sem etiologia definida. Após anos estável, apresentou piora grave, necessitando implantar um ressincronizador cardíaco. Conclusão: A Miocardiopatia Dilatada é uma doença multifatorial, com sintomatologia variável, que dificulta seu diagnóstico rápido e definição da etiologia, influenciando no prognóstico e bem-estar do paciente.
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