1,355 research outputs found
A codon-optimized green fluorescent protein for live cell imaging in Zymoseptoria tritici
AbstractFluorescent proteins (FPs) are powerful tools to investigate intracellular dynamics and protein localization. Cytoplasmic expression of FPs in fungal pathogens allows greater insight into invasion strategies and the host-pathogen interaction. Detection of their fluorescent signal depends on the right combination of microscopic setup and signal brightness. Slow rates of photo-bleaching are pivotal for in vivo observation of FPs over longer periods of time. Here, we test green-fluorescent proteins, including Aequorea coerulescens GFP (AcGFP), enhanced GFP (eGFP) from Aequorea victoria and a novel Zymoseptoria tritici codon-optimized eGFP (ZtGFP), for their usage in conventional and laser-enhanced epi-fluorescence, and confocal laser-scanning microscopy. We show that eGFP, expressed cytoplasmically in Z. tritici, is significantly brighter and more photo-stable than AcGFP. The codon-optimized ZtGFP performed even better than eGFP, showing significantly slower bleaching and a 20–30% further increase in signal intensity. Heterologous expression of all GFP variants did not affect pathogenicity of Z. tritici. Our data establish ZtGFP as the GFP of choice to investigate intracellular protein dynamics in Z. tritici, but also infection stages of this wheat pathogen inside host tissue
Effects of size at birth, childhood growth patterns and growth hormone treatment on leukocyte telomere length
__Background__ Small size at birth and rapid growth in early life are associated with increased risk of cardiovascular disease in later life. Short children born small for gestational age (SGA) are treated with growth hormone (GH), inducing catch-up in length. Leukocyte telomere length (LTL) is a marker of biological age and shorter LTL is associated with increased risk of cardiovascular disease.
__Objectives__ To investigate whether LTL is influenced by birth size, childhood growth and long-term GH treatment.
__Methods__ We analyzed LTL in 545 young adults with differences in birth size and childhood growth patterns. Previously GH-treated young adults born SGA (SGA-GH) were compared to untreated short SGA (SGA-S), SGA with spontaneous catch-up to a normal body size (SGA-CU), and appropriate for gestational age with a normal body size (AGA-NS). LTL was measured using a quantitative PCR assay.
__Results__ We found a positive association between birth length and LTL (p = 0.04), and a trend towards a positive association between birth weight and LTL (p = 0.08), after adjustments for gender, age, gestational age and adult body size. Weight gain during infancy and childhood and fat mass percentage were not as
Holographic principle in the BDL brane cosmology
We study the holographic principle in the brane cosmology. Especially we
describe how to accommodate the 5D anti de Sitter Schwarzschild (AdSS)
black hole in the Binetruy-Deffayet-Langlois (BDL) approach of brane cosmology.
It is easy to make a connection between a mass of the AdSS black hole
and a conformal field theory (CFT)-radiation dominated universe on the brane in
the moving domain wall approach. But this is not established in the BDL
approach. In this case we use two parameters in the Friedmann
equation. These arise from integration and are really related to the choice of
initial bulk matter. If one chooses a bulk energy density to account
for a mass of the AdSS black hole and the static fifth dimension, a
CFT-radiation term with comes out from the bulk
matter without introducing a localized matter distribution on the brane. This
means that the holographic principle can be established in the BDL brane
cosmology.Comment: 9 pages, a version to appear in PR
Radio Bursts Associated with Flare and Ejecta in the 13 July 2004 Event
We investigate coronal transients associated with a GOES M6.7 class flare and
a coronal mass ejection (CME) on 13 July 2004. During the rising phase of the
flare, a filament eruption, loop expansion, a Moreton wave, and an ejecta were
observed. An EIT wave was detected later on. The main features in the radio
dynamic spectrum were a frequency-drifting continuum and two type II bursts.
Our analysis shows that if the first type II burst was formed in the low
corona, the burst heights and speed are close to the projected distances and
speed of the Moreton wave (a chromospheric shock wave signature). The
frequency-drifting radio continuum, starting above 1 GHz, was formed almost two
minutes prior to any shock features becoming visible, and a fast-expanding
piston (visible as the continuum) could have launched another shock wave. A
possible scenario is that a flare blast overtook the earlier transient, and
ignited the first type II burst. The second type II burst may have been formed
by the same shock, but only if the shock was propagating at a constant speed.
This interpretation also requires that the shock-producing regions were located
at different parts of the propagating structure, or that the shock was passing
through regions with highly different atmospheric densities. This complex
event, with a multitude of radio features and transients at other wavelengths,
presents evidence for both blast-wave-related and CME-related radio emissions.Comment: 14 pages, 6 figures; Solar Physics Topical Issue, in pres
Frozen spatial chaos induced by boundaries
We show that rather simple but non-trivial boundary conditions could induce
the appearance of spatial chaos (that is stationary, stable, but spatially
disordered configurations) in extended dynamical systems with very simple
dynamics. We exemplify the phenomenon with a nonlinear reaction-diffusion
equation in a two-dimensional undulated domain. Concepts from the theory of
dynamical systems, and a transverse-single-mode approximation are used to
describe the spatially chaotic structures.Comment: 9 pages, 6 figures, submitted for publication; for related work visit
http://www.imedea.uib.es/~victo
Recent developments in planet migration theory
Planetary migration is the process by which a forming planet undergoes a
drift of its semi-major axis caused by the tidal interaction with its parent
protoplanetary disc. One of the key quantities to assess the migration of
embedded planets is the tidal torque between the disc and planet, which has two
components: the Lindblad torque and the corotation torque. We review the latest
results on both torque components for planets on circular orbits, with a
special emphasis on the various processes that give rise to additional, large
components of the corotation torque, and those contributing to the saturation
of this torque. These additional components of the corotation torque could help
address the shortcomings that have recently been exposed by models of planet
population syntheses. We also review recent results concerning the migration of
giant planets that carve gaps in the disc (type II migration) and the migration
of sub-giant planets that open partial gaps in massive discs (type III
migration).Comment: 52 pages, 18 figures. Review article to be published in "Tidal
effects in Astronomy and Astrophysics", Lecture Notes in Physic
Gastric cancer and Helicobacter pylori: a combined analysis of 12 case control studies nested within prospective cohorts
BACKGROUND: The magnitude of the association
between Helicobacter pylori and
incidence of gastric cancer is unclear. H
pylori infection and the circulating antibody
response can be lost with development
of cancer; thus retrospective studies
are subject to bias resulting from classifi-
cation of cases as H pylori negative when
they were infected in the past.
AIMS: To combine data from all case control
studies nested within prospective
cohorts to assess more reliably the relative
risk of gastric cancer associated with H
pylori infection.To investigate variation in
relative risk by age, sex, cancer type and
subsite, and interval between blood sampling
and cancer diagnosis.
METHODS: Studies were eligible if blood
samples for H pylori serology were collected
before diagnosis of gastric cancer in
cases. Identified published studies and two
unpublished studies were included. Individual
subject data were obtained for
each. Matched odds ratios (ORs) and 95%
confidence intervals (95% CI) were calculated
for the association between H pylori
and gastric cancer.
RESULTS: Twelve studies with 1228 gastric
cancer cases were considered. The association
with H pylori was restricted to noncardia
cancers (OR 3.0; 95% CI 2.3–3.8)
and was stronger when blood samples for
H pylori serology were collected 10+ years
before cancer diagnosis (5.9; 3.4–10.3). H
pylori infection was not associated with an
altered overall risk of cardia cancer (1.0;
0.7–1.4).
CONCLUSIONS: These results suggest that
5.9 is the best estimate of the relative risk
of non-cardia cancer associated with H
pylori infection and that H pylori does not
increase the risk of cardia cancer. They
also support the idea that when H pylori
status is assessed close to cancer diagnosis,
the magnitude of the non-cardia
association may be underestimated
Structure Formation, Melting, and the Optical Properties of Gold/DNA Nanocomposites: Effects of Relaxation Time
We present a model for structure formation, melting, and optical properties
of gold/DNA nanocomposites. These composites consist of a collection of gold
nanoparticles (of radius 50 nm or less) which are bound together by links made
up of DNA strands. In our structural model, the nanocomposite forms from a
series of Monte Carlo steps, each involving reaction-limited cluster-cluster
aggregation (RLCA) followed by dehybridization of the DNA links. These links
form with a probability which depends on temperature and particle
radius . The final structure depends on the number of monomers (i. e. gold
nanoparticles) , , and the relaxation time. At low temperature, the
model results in an RLCA cluster. But after a long enough relaxation time, the
nanocomposite reduces to a compact, non-fractal cluster. We calculate the
optical properties of the resulting aggregates using the Discrete Dipole
Approximation. Despite the restructuring, the melting transition (as seen in
the extinction coefficient at wavelength 520 nm) remains sharp, and the melting
temperature increases with increasing as found in our previous
percolation model. However, restructuring increases the corresponding link
fraction at melting to a value well above the percolation threshold. Our
calculated extinction cross section agrees qualitatively with experiments on
gold/DNA composites. It also shows a characteristic ``rebound effect,''
resulting from incomplete relaxation, which has also been seen in some
experiments. We discuss briefly how our results relate to a possible sol-gel
transition in these aggregates.Comment: 12 pages, 10 figure
Functional diversity of chemokines and chemokine receptors in response to viral infection of the central nervous system.
Encounters with neurotropic viruses result in varied outcomes ranging from encephalitis, paralytic poliomyelitis or other serious consequences to relatively benign infection. One of the principal factors that control the outcome of infection is the localized tissue response and subsequent immune response directed against the invading toxic agent. It is the role of the immune system to contain and control the spread of virus infection in the central nervous system (CNS), and paradoxically, this response may also be pathologic. Chemokines are potent proinflammatory molecules whose expression within virally infected tissues is often associated with protection and/or pathology which correlates with migration and accumulation of immune cells. Indeed, studies with a neurotropic murine coronavirus, mouse hepatitis virus (MHV), have provided important insight into the functional roles of chemokines and chemokine receptors in participating in various aspects of host defense as well as disease development within the CNS. This chapter will highlight recent discoveries that have provided insight into the diverse biologic roles of chemokines and their receptors in coordinating immune responses following viral infection of the CNS
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