9 research outputs found
Incidence, predictors and criteria for the diagnosis of giardiasis in children
Background. The prevalence of giardiasis among children is 355 cases per 100,000, with 80 % of cases of infestation occurring in children under the age of 14 years. The urgency of the high prevalence of protozoal pathology in children is determined by the fact that its manifestations are often masked under various pathological conditions. The purpose was to study the incidence, predictors and criteria for the diagnosis of giardiasis in children. Materials and methods. Research design is a controlled randomized trial. The study included children suffering from giardiasis (nΒ = 55), aged 3 to 17 years. The comparison group consisted of 30 apparently healthy children. A survey of patients and collection of material performed at the outpatient and gastroenterology departments of the MHI βChernivtsi Regional Clinical Hospitalβ during 2014β2016. Laboratory and instrumental investigations were conducted in Ukrainian-German laboratory βBukintermedβ and childrenβs gastroenterology center βDigestβ. Indirect (serological one, macroscopy of the duodenal mucosa in endoscopic examination, indirect signs of giardiasis in duodenal biopsy samples) and direct methods were used (microscopy of the feces, duodenal contents, detection of vegetative forms of lamblia in duodenal biopsy samples during histological examination). A parent survey was conducted. Results. A positive result was obtained in 29 out of 565 subjects surveyed for lamblia (5.1 %). More often, additional examination was required for the presence of giardiasis in children suffering from chronic diseases of the upper gastrointestinal and hepatobiliary system, allergic reactions, intestinal dysbiosis and persistent eosinophilia. Children affected with giardiasis were more likely to have a concomitant illness than the children of the comparison group (p < 0.05). In 41.4 % of cases, children lived in conditions that were not satisfactory, did not always follow the rules of sanitary hygiene. We have established antenatal (health during pregnancy, the course of pregnancy, the presence of occupational hazards), intranatal (premature birth, stimulation of labor and cesarean section, birth trauma and asphyxia in children) and postnatal adverse factors (duration of breastfeeding, concomitant pathology). All histological features can be used as possible criteria for giardiasis in children, but with the highest probability, the following sign can be used β the presence of villous epithelium desquamation areas with simultaneous dominance of eosinophils and mast cells in the infiltrate. Conclusions. The incidence of giardiasis in children and adolescents is 5.1 %. Anamnestic factors (social, hygienic, medical and biological ones) established by us can be considered as predictors for the development of giardiasis in children. In the clinical picture of giardiasis, the defeat of the digestive tract dominates β in 96.3 % of children. To improve the accuracy of giardiasis diagnosis in children, it is necessary to use a set of direct and indirect methods of the diagnosis
Incidence, predictors and criteria for the diagnosis of giardiasis in children
ΠΠ΅ΡΠ°. ΠΠΈΠ²ΡΠ΅Π½Π½Ρ ΡΠ°ΡΡΠΎΡΠΈ, ΠΏΡΠ΅Π΄ΠΈΠΊΡΠΎΡΡΠ² Ρ ΠΊΡΠΈΡΠ΅ΡΡΡΠ² Π΄ΡΠ°Π³Π½ΠΎΡΡΠΈΠΊΠΈ Π»ΡΠΌΠ±Π»ΡΠΎΠ·Ρ Π² Π΄ΡΡΠ΅ΠΉ.
ΠΠΈΡΠ½ΠΎΠ²ΠΊΠΈ. Π§Π°ΡΡΠΎΡΠ° Π»ΡΠΌΠ±Π»ΡΠΎΠ·Ρ Ρ Π΄ΡΡΠ΅ΠΉ Ρ ΠΏΡΠ΄Π»ΡΡΠΊΡΠ² Π΄ΠΎΡΡΠ²Π½ΡΡ 5,1 %. ΠΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½Ρ Π½Π°ΠΌΠΈ Π°Π½Π°ΠΌΠ½Π΅ΡΡΠΈΡΠ½Ρ ΡΠ°ΠΊΡΠΎΡΠΈ (ΡΠΎΡΡΠ°Π»ΡΠ½Ρ, Π³ΡΠ³ΡΡΠ½ΡΡΠ½Ρ, ΠΌΠ΅Π΄ΠΈΠΊΠΎ-Π±ΡΠΎΠ»ΠΎΠ³ΡΡΠ½Ρ) ΠΌΠΎΠΆΠ½Π° ΡΠΎΠ·Π³Π»ΡΠ΄Π°ΡΠΈ ΡΠΊ ΠΏΡΠ΅Π΄ΠΈΠΊΡΠΎΡΠΈ ΡΠΎΠ·Π²ΠΈΡΠΊΡ Π»ΡΠΌΠ±Π»ΡΠΎΠ·Ρ Π² Π΄ΡΡΠ΅ΠΉ. Π£ ΠΊΠ»ΡΠ½ΡΡΡ Π»ΡΠΌΠ±Π»ΡΠΎΠ·Ρ Π΄ΠΎΠΌΡΠ½ΡΡ ΡΡΠ°ΠΆΠ΅Π½Π½Ρ ΡΡΠ°Π²Π½ΠΎΠ³ΠΎ ΡΡΠ°ΠΊΡΡ β Ρ 96,3 % Π΄ΡΡΠ΅ΠΉ. ΠΠ»Ρ ΠΏΡΠ΄Π²ΠΈΡΠ΅Π½Π½Ρ ΡΠΎΡΠ½ΠΎΡΡΡ Π΄ΡΠ°Π³Π½ΠΎΡΡΠΈΠΊΠΈ Π»ΡΠΌΠ±Π»ΡΠΎΠ·Ρ Π² Π΄ΡΡΠ΅ΠΉ Π½Π΅ΠΎΠ±Ρ
ΡΠ΄Π½ΠΎ Π²ΠΈΠΊΠΎΡΠΈΡΡΠΎΠ²ΡΠ²Π°ΡΠΈ ΠΊΠΎΠΌΠΏΠ»Π΅ΠΊΡ ΠΏΡΡΠΌΠΈΡ
Ρ Π½Π΅ΠΏΡΡΠΌΠΈΡ
ΠΌΠ΅ΡΠΎΠ΄ΡΠ² Π΄ΡΠ°Π³Π½ΠΎΡΡΠΈΠΊΠΈ.
Π¦Π΅Π»Ρ. ΠΠ·ΡΡΠ΅Π½ΠΈΠ΅ ΡΠ°ΡΡΠΎΡΡ, ΠΏΡΠ΅Π΄ΠΈΠΊΡΠΎΡΠΎΠ² ΠΈ ΠΊΡΠΈΡΠ΅ΡΠΈΠ΅Π² Π΄ΠΈΠ°Π³Π½ΠΎΡΡΠΈΠΊΠΈ Π»ΡΠΌΠ±Π»ΠΈΠΎΠ·Π° Ρ Π΄Π΅ΡΠ΅ΠΉ.
ΠΡΠ²ΠΎΠ΄Ρ. Π§Π°ΡΡΠΎΡΠ° Π»ΡΠΌΠ±Π»ΠΈΠΎΠ·Π° Ρ Π΄Π΅ΡΠ΅ΠΉ ΠΈ ΠΏΠΎΠ΄ΡΠΎΡΡΠΊΠΎΠ² ΡΠΎΡΡΠ°Π²Π»ΡΠ΅Ρ 5,1 %. ΠΠ½Π°ΠΌΠ½Π΅ΡΡΠΈΡΠ΅ΡΠΊΠΈΠ΅ ΡΠ°ΠΊΡΠΎΡΡ (ΡΠΎΡΠΈΠ°Π»ΡΠ½ΡΠ΅, Π³ΠΈΠ³ΠΈΠ΅Π½ΠΈΡΠ΅ΡΠΊΠΈΠ΅, ΠΌΠ΅Π΄ΠΈΠΊΠΎ-Π±ΠΈΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΠ΅) ΠΌΠΎΠΆΠ½ΠΎ ΡΠ°ΡΡΠΌΠ°ΡΡΠΈΠ²Π°ΡΡ ΠΊΠ°ΠΊ ΠΏΡΠ΅Π΄ΠΈΠΊΡΠΎΡΡ ΡΠ°Π·Π²ΠΈΡΠΈΡ Π»ΡΠΌΠ±Π»ΠΈΠΎΠ·Π° Ρ Π΄Π΅ΡΠ΅ΠΉ. Π ΠΊΠ»ΠΈΠ½ΠΈΠΊΠ΅ Π»ΡΠΌΠ±Π»ΠΈΠΎΠ·Π° Π΄ΠΎΠΌΠΈΠ½ΠΈΡΡΡΡ ΠΏΠΎΡΠ°ΠΆΠ΅Π½ΠΈΡ ΠΏΠΈΡΠ΅Π²Π°ΡΠΈΡΠ΅Π»ΡΠ½ΠΎΠ³ΠΎ ΡΡΠ°ΠΊΡΠ° (Ρ 96,3 % Π΄Π΅ΡΠ΅ΠΉ). ΠΠ»Ρ ΠΏΠΎΠ²ΡΡΠ΅Π½ΠΈΡ ΡΠΎΡΠ½ΠΎΡΡΠΈ Π΄ΠΈΠ°Π³Π½ΠΎΡΡΠΈΠΊΠΈ Π»ΡΠΌΠ±Π»ΠΈΠΎΠ·Π° Ρ Π΄Π΅ΡΠ΅ΠΉ Π½Π΅ΠΎΠ±Ρ
ΠΎΠ΄ΠΈΠΌΠΎ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°ΡΡ ΠΊΠΎΠΌΠΏΠ»Π΅ΠΊΡ ΠΏΡΡΠΌΡΡ
ΠΈ ΠΊΠΎΡΠ²Π΅Π½Π½ΡΡ
ΠΌΠ΅ΡΠΎΠ΄ΠΎΠ² Π΄ΠΈΠ°Π³Π½ΠΎΡΡΠΈΠΊΠΈ.
The purpose was to study the incidence, predictors and criteria for the diagnosis of giardiasis in children.
Conclusions. The incidence of giardiasis in children and adolescents is 5.1 %. Anamnestic factors (social, hygienic, medical and biological ones) established by us can be considered as predictors for the development of giardiasis in children. In the clinical picture of giardiasis, the defeat of the digestive tract dominates β in 96.3 % of children. To improve the accuracy of giardiasis diagnosis in children, it is necessary to use a set of direct and indirect methods of the diagnosis
The Functions of RNA-Dependent RNA Polymerases in Arabidopsis
One recently identified mechanism that regulates mRNA abundance is RNA silencing, and pioneering work in Arabidopsis thaliana and other genetic model organisms helped define this process. RNA silencing pathways are triggered by either self-complementary fold-back structures or the production of double-stranded RNA (dsRNA) that gives rise to small RNAs (smRNAs) known as microRNAs (miRNAs) or small-interfering RNAs (siRNAs). These smRNAs direct sequence-specific regulation of various gene transcripts, repetitive sequences, viruses, and mobile elements via RNA cleavage, translational inhibition, or transcriptional silencing through DNA methylation and heterochromatin formation. Early genetic screens in Arabidopsis were instrumental in uncovering numerous proteins required for these important regulatory pathways. Among the factors identified by these studies were RNA-dependent RNA polymerases (RDRs), which are proteins that synthesize siRNA-producing dsRNA molecules using a single-stranded RNA (ssRNA) molecule as a template. Recently, a growing body of evidence has implicated RDR-dependent RNA silencing in many different aspects of plant biology ranging from reproductive development to pathogen resistance. Here, we focus on the specific functions of the six Arabidopsis RDRs in RNA silencing, their ssRNA substrates and resulting RDR-dependent smRNAs, and the numerous biological functions of these proteins in plant development and stress responses