3,530 research outputs found
Inducible changes in cell size and attachment area due to expression of a mutant SWI/SNF chromatin remodeling enzyme
The SWI/SNF enzymes belong to a family of ATP-dependent chromatin remodeling enzymes that have been functionally implicated in gene regulation, development, differentiation and oncogenesis. BRG1, the catalytic core subunit of some of the SWI/SNF enzymes, can interact with known tumor suppressor proteins and can act as a tumor suppressor itself. We report that cells that inducibly express ATPase-deficient versions of BRG1 increase in cell volume, area of attachment and nuclear size upon expression of the mutant BRG1 protein. Examination of focal adhesions reveals qualitative changes in paxillin distribution but no difference in the actin cytoskeletal structure. Increases in cell size and shape correlate with over-expression of two integrins and the urokinase-type plasminogen activator receptor (uPAR), which is also involved in cell adhesion and is often over-expressed in metastatic cancer cells. These findings demonstrate that gene expression pathways affected by chromatin remodeling enzymes can regulate the physical dimensions of mammalian cell morphology
First annotated draft genomes of nonmarine ostracods (Ostracoda, Crustacea) with different reproductive modes.
Ostracods are one of the oldest crustacean groups with an excellent fossil record and high importance for phylogenetic analyses but genome resources for this class are still lacking. We have successfully assembled and annotated the first reference genomes for three species of nonmarine ostracods; two with obligate sexual reproduction (Cyprideis torosa and Notodromas monacha) and the putative ancient asexual Darwinula stevensoni. This kind of genomic research has so far been impeded by the small size of most ostracods and the absence of genetic resources such as linkage maps or BAC libraries that were available for other crustaceans. For genome assembly, we used an Illumina-based sequencing technology, resulting in assemblies of similar sizes for the three species (335-382 Mb) and with scaffold numbers and their N50 (19-56 kb) in the same orders of magnitude. Gene annotations were guided by transcriptome data from each species. The three assemblies are relatively complete with BUSCO scores of 92-96. The number of predicted genes (13,771-17,776) is in the same range as Branchiopoda genomes but lower than in most malacostracan genomes. These three reference genomes from nonmarine ostracods provide the urgently needed basis to further develop ostracods as models for evolutionary and ecological research
Isospin dependence of relative yields of and mesons at 1.528 AGeV
Results on and meson production in Ru +
Ru and Zr + Zr collisions at a beam kinetic
energy of 1.528 GeV, measured with the FOPI detector at GSI-Darmstadt, are
investigated as a possible probe of isospin effects in high density nuclear
matter. The measured double ratio ()/() is
compared to the predictions of a thermal model and a Relativistic Mean Field
transport model using two different collision scenarios and under different
assumptions on the stiffness of the symmetry energy. We find a good agreement
with the thermal model prediction and the assumption of a soft symmetry energy
for infinite nuclear matter while more realistic transport simulations of the
collisions show a similar agreement with the data but also exhibit a reduced
sensitivity to the symmetry term.Comment: 5 pages, 3 figures. accepted for publication in Phys. Rev.
Strange meson production in Al+Al collisions at 1.9A GeV
The production of K, K and (1020) mesons is studied in Al+Al
collisions at a beam energy of 1.9A GeV which is close or below the production
threshold in NN reactions. Inverse slopes, anisotropy parameters, and total
emission yields of K mesons are obtained. A comparison of the ratio of
kinetic energy distributions of K and K mesons to the HSD transport
model calculations suggests that the inclusion of the in-medium modifications
of kaon properties is necessary to reproduce the ratio. The inverse slope and
total yield of mesons are deduced. The contribution to K production
from meson decays is found to be [17 3 (stat) (syst)]
%. The results are in line with previous K and data obtained for
different colliding systems at similar incident beam energies.Comment: 16 pages, 11 figure
Centrality dependence of subthreshold meson production in Ni+Ni collisions at 1.9A GeV
We analysed the meson production in central Ni+Ni collisions at the
beam kinetic energy of 1.93A GeV with the FOPI spectrometer and found the
production probability per event of . This new data point allows for the first time
to inspect the centrality dependence of the subthreshold meson
production in heavy-ion collisions. The rise of meson multiplicity per
event with mean number of participants can be parameterized by the power
function with exponent . The ratio of to
production yields seems not to depend within the experimental
uncertainties on the collision centrality, and the average of measured values
was found to be .Comment: 9 pages, 5 figure
Measurement of and mesons in Al+Al collisions at 1.9 GeV
New measurement of sub-threshold and production is
presented. The experimental data complete the measurement of strange particles
produced in Al+Al collisions at 1.9 GeV measured with the FOPI detector at
SIS/GSI. The / yield ratio is found to be and is in good agreement with the
UrQMD model prediction. These measurements provide information on in-medium
cross section of - fusion which is the dominant process on
sub-threshold production.Comment: 5 pages, 4 figures, accepted for publication in Phys. Rev.
First analysis of anisotropic flow with Lee--Yang zeroes
We report on the first analysis of directed and elliptic flow with the new
method of Lee--Yang zeroes. Experimental data are presented for Ru+Ru reactions
at 1.69 AGeV measured with the FOPI detector at SIS/GSI. The results obtained
with several methods, based on the event-plane reconstruction, on Lee--Yang
zeroes, and on multi-particle cumulants (up to 5th order) applied for the first
time at SIS energies, are compared. They show conclusive evidence that
azimuthal correlations between nucleons and composite particles at this energy
are largely dominated by anisotropic flow.Comment: 5 pages, 3 figures, submitted to Phys. Rev. C Rapid Co
Direct comparison of phase-space distributions of K- and K+ mesons in heavy-ion collisions at SIS energies - evidence for in-medium modifications of kaons ?
The ratio of K- to K+ meson yields has been measured in the systems RuRu at
1.69 A GeV, Ru+Zr at 1.69 A GeV, and Ni+Ni at 1.93 A GeV incident beam kinetic
energy. The yield ratio is observed to vary across the measured phase space.
Relativistic transport-model calculations indicate that the data are best
understood if in-medium modifications of the kaons are taken into account.Comment: 14 pages including 3 figure
Two-proton small-angle correlations in central heavy-ion collisions: a beam-energy and system-size dependent study
Small-angle correlations of pairs of protons emitted in central collisions of
Ca + Ca, Ru + Ru and Au + Au at beam energies from 400 to 1500 MeV per nucleon
are investigated with the FOPI detector system at SIS/GSI Darmstadt.
Dependences on system size and beam energy are presented which extend the
experimental data basis of pp correlations in the SIS energy range
substantially. The size of the proton-emitting source is estimated by comparing
the experimental data with the output of a final-state interaction model which
utilizes either static Gaussian sources or the one-body phase-space
distribution of protons provided by the BUU transport approach. The trends in
the experimental data, i.e. system-size and beam energy dependences, are well
reproduced by this hybrid model. However, the pp correlation function is found
rather insensitive to the stiffness of the equation of state entering the
transport model calculations.Comment: 9 pages, 8 figures, accepted at Eur. Phys. Journ.
Multistrip multigap symmetric RPC
Abstract The characteristics of a symmetric multigap resistive plate chamber with multistrip readout electrode, recently developed by us, continued to be investigated. Studies of the time resolution, efficiency, average charge and dark rate as a function of applied voltage and the influence of the angle of incidence of the detected particle on these observables have been performed. Different type of discriminators have been tested
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