Resistance traits and AFLP characterization of diploid primitive tuber-bearing potatoes.

ISSN: 0925-986

On the stability conditions for theories of modified gravity in the presence of matter fields

Theoretical Physic

Cosmological constraints and phenomenology of a beyond-Horndeski model

We study observational constraints on a specific dark energy model in the framework of Gleyzes-Langlois-Piazza-Vernizzi theories, which extends the Galileon ghost condensate (GGC) to the domain of beyond Horndeski theories. In this model, we show that the Planck cosmic microwave background (CMB) data, combined with datasets of baryon acoustic oscillations, supernovae type Ia, and redshift-space distortions, give the tight upper bound |α(0)H|≤O(10−6) on today’s beyond-Horndeski (BH) parameter αH. This is mostly attributed to the shift of CMB acoustic peaks induced by the early-time changes of cosmological background and perturbations arising from the dominance of αH in the dark energy density. In comparison to the Λ cold dark matter (ΛCDM) model, our BH model suppresses the large-scale integrated-Sachs-Wolfe tail of CMB temperature anisotropies due to the existence of cubic Galileons, and it modifies the small-scale CMB power spectrum because of the different background evolution. We find that the BH model considered fits the data better than ΛCDM according to the χ2 statistics, yet the deviance information criterion (DIC) slightly favors the latter. Given the fact that our BH model with αH=0 (i.e., the GGC model) is favored over ΛCDM even by the DIC, there are no particular signatures for the departure from Horndeski theories in current observations.Theoretical Physic

Dissecting kinetically coupled quintessence: phenomenology and observational tests

We investigate an interacting dark energy model which allows for the kinetic term of the scalar field to couple to dark matter via a power-law interaction. The model is characterised by scaling solutions at early times, which are of high interest to alleviate the coincidence problem, followed by a period of accelerated expansion. We discuss the phenomenology of the background evolution and of the linear scalar perturbations and we identify measurable signatures of the coupling in the dark sector on the cosmic microwave background, the lensing potential auto-correlation and the matter power spectra. We also perform a parameter estimation analysis using data of cosmic microwave background temperature, polarisation and lensing, baryonic acoustic oscillations and supernovae. We find that the strength of the coupling between the dark sectors, regulated by the parameter α, is constrained to be of order 10-4. A model selection analysis does not reveal a statistical preference between ΛCDM and the Kinetic model

Cosmological data favor Galileon ghost condensate over Λ CDM

We place observational constraints on the Galileon ghost condensate model, a dark energy proposal in cubic-order Horndeski theories consistent with the gravitational-wave event GW170817. The model extends the covariant Galileon by taking an additional higher-order field derivative X2 into account. This allows for the dark energy equation of state wDE to access the region −2Theoretical Physic

A novel synthetic peptide from a tomato defensin exhibits antibacterial activities against Helicobacter pylori

Defensins are a class of cysteine-rich proteins, which exert broad spectrum antimicrobial activity. In this work, we used a bioinformatic approach to identify putative defensins in the tomato genome. Fifteen proteins had a mature peptide that includes the well-conserved tetradisulfide array. We selected a representative member of the tomato defensin family; we chemically synthesized its gamma-motif and tested its antimicrobial activity. Here, we demonstrate that the synthetic peptide exhibits potent antibacterial activity against Gram-positive bacteria, such as Staphylococcus aureus A170, Staphylococcus epidermidis, and Listeria monocytogenes, and Gram-negative bacteria, including Salmonella enterica serovar Paratyphi, Escherichia coli, and Helicobacter pylori. In addition, the synthetic peptide shows minimal (<5%) hemolytic activity and absence of cytotoxic effects against THP-1 cells. Finally, SolyC exerts an anti-inflammatory activity in vitro, as it downregulates the level of the proinflammatory cytokines TNF-alpha and IFN-gamma

Secondary metabolite profile in induced tetraploids of wild Solanum commersonii Dun.

The main aim of this work was to study the leaf secondary metabolite profiles of artificially induced tetraploids (2n1⁄44x1⁄448) of Solanum commersonii, a diploid (2n1⁄42x1⁄424) wild potato species. The tetraploid genotypes of S. commersonii were produced by oryzalin treatment. Both HPLC-UV and LC/ MS analyses revealed that there were no qualitative differences in the metabolite profiles between the diploid S. commersonii and its tetraploids. By contrast, the results showed that the phenylpropanoid content was generally significantly higher in the tetraploids than in the diploid S. commersonii. Concerning the glycoalkaloids (GAs), the results provided evidence that the content of minor GAs (solanidenediol triose, solanidadienol lycotetraose, and solanidenol lycotetraose) was higher in tetraploids than in the diploid progenitor, while the content of major GAs (dehydrodemissine and dehydrocommersonine) was significantly higher in diploid S. commersonii than in its tetraploid genotypes. The results are discussed from the practical perspective of potato biodiversity enhancement

The Distribution of Mass in the Orion Dwarf Galaxy

Dwarf galaxies are good candidates to investigate the nature of Dark Matter, because their kinematics are dominated by this component down to small galactocentric radii. We present here the results of detailed kinematic analysis and mass modelling of the Orion dwarf galaxy, for which we derive a high quality and high resolution rotation curve that contains negligible non-circular motions and we correct it for the asymmetric drift. Moreover, we leverage the proximity (D = 5.4 kpc) and convenient inclination (47{\deg}) to produce reliable mass models of this system. We find that the Universal Rotation Curve mass model (Freeman disk + Burkert halo + gas disk) fits the observational data accurately. In contrast, the NFW halo + Freeman disk + gas disk mass model is unable to reproduce the observed Rotation Curve, a common outcome in dwarf galaxies. Finally, we attempt to fit the data with a MOdified Newtonian Dynamics (MOND) prescription. With the present data and with the present assumptions on distance, stellar mass, constant inclination and reliability of the gaseous mass, the MOND "amplification" of the baryonic component appears to be too small to mimic the required "dark component". The Orion dwarf reveals a cored DM density distribution and a possible tension between observations and the canonical MOND formalism.Comment: 8 pages, 9 figures, accepted for publication in MNRA

ISOL@: an Italian SOLAnaceae genomics resource

BACKGROUND: Present-day '-omics' technologies produce overwhelming amounts of data which include genome sequences, information on gene expression (transcripts and proteins) and on cell metabolic status. These data represent multiple aspects of a biological system and need to be investigated as a whole to shed light on the mechanisms which underpin the system functionality.The gathering and convergence of data generated by high-throughput technologies, the effective integration of different data-sources and the analysis of the information content based on comparative approaches are key methods for meaningful biological interpretations.In the frame of the International Solanaceae Genome Project, we propose here ISOLA, an Italian SOLAnaceae genomics resource. RESULTS: ISOLA (available at http://biosrv.cab.unina.it/isola) represents a trial platform and it is conceived as a multi-level computational environment.ISOLA currently consists of two main levels: the genome and the expression level. The cornerstone of the genome level is represented by the Solanum lycopersicum genome draft sequences generated by the International Tomato Genome Sequencing Consortium. Instead, the basic element of the expression level is the transcriptome information from different Solanaceae species, mainly in the form of species-specific comprehensive collections of Expressed Sequence Tags (ESTs).The cross-talk between the genome and the expression levels is based on data source sharing and on tools that enhance data quality, that extract information content from the levels' under parts and produce value-added biological knowledge. CONCLUSIONS: ISOLA is the result of a bioinformatics effort that addresses the challenges of the post-genomics era. It is designed to exploit '-omics' data based on effective integration to acquire biological knowledge and to approach a systems biology view. Beyond providing experimental biologists with a preliminary annotation of the tomato genome, this effort aims to produce a trial computational environment where different aspects and details are maintained as they are relevant for the analysis of the organization, the functionality and the evolution of the Solanaceae family

Use of SSR and retrotransposon-based markers to interpret the population structure of native grapevines from Southern Italy

Native grapevines are the quintessential ele- ments of Southern Italy winemaking, and genomic char- acterization plays a role of primary importance for preservation and sustainable use of these unexploited genetic resources. Among the various molecular techniques available, SSR and retrotransposons-based markers result to be the most valuable for cultivars and biotypes distinc- tiveness. A total of 62 accessions including 38 local grape cultivars were analyzed with 30 SSR, four REMAP and one IRAP markers to assess their genetic diversity and obtain a complete genomic profiling. The use of VrZAG79, VrZAG112, VVS2, VVMD25 and VVMD5 combined with retrotransposon-based markers proved to be the most dis- criminating and polymorphic markers for the rapid and unambiguous identification of minority grapevines from Campania region, which is considered one of the most appreciated Italian districts for wine production. Results revealed 58 SSR marker-specific alleles, 22 genotype- specific SSR alleles, and four REMAP and IRAP private bands. Cases of synonymy and homonymy were discov- ered. In conclusion, we provided evidences that the inte- grating SSR and retrotransposon-based markers is an effective strategy to assess the genetic diversity of autochthonous grapes, allowing their easy identification