Modelling fixed plant and algal dynamics in rivers: an application to the River Frome

The development of eutrophication in river systems is poorly understood given the complex relationship between fixed plants, algae, hydrodynamics, water chemistry and solar radiation. However there is a pressing need to understand the relationship between the ecological status of rivers and the controlling environmental factors to help the reasoned implementation of the Water Framework Directive and Catchment Sensitive Farming in the UK. This research aims to create a dynamic, process-based, mathematical in-stream model to simulate the growth and competition of different vegetation types (macrophytes, phytoplankton and benthic algae) in rivers. The model, applied to the River Frome (Dorset, UK), captured well the seasonality of simulated vegetation types (suspended algae, macrophytes, epiphytes, sediment biofilm). Macrophyte results showed that local knowledge is important for explaining unusual changes in biomass. Fixed algae simulations indicated the need for the more detailed representation of various herbivorous grazer groups, however this would increase the model complexity, the number of model parameters and the required observation data to better define the model. The model results also highlighted that simulating only phytoplankton is insufficient in river systems, because the majority of the suspended algae have benthic origin in short retention time rivers. Therefore, there is a need for modelling tools that link the benthic and free-floating habitats

Characterization of PPAR-gamma 1 and PPAR-gamma 2 in Knockin and Knockout Mouse Models

The global epidemic of obesity and type II diabetes has led to a growing interest in the underlying mechanisms of metabolic diseases. The peroxisome proliferator-activated receptor gamma (PPARγ) is a member of the nuclear receptor superfamily, and is vital for the transcriptional regulation of adipogenesis, insulin sensitivity and lipid metabolism. In the mouse model, it has been demonstrated that global knockout of PPARγ leads to severe metabolic disturbance, resulting in embryonic lethality. However, the specific regulatory roles of its two protein isoforms, PPARγ1 and PPARγ2, remain uncertain, due to limitations of reagents and appropriate mouse models. To investigate the hypothesis that PPARγ1 and PPARγ2 are functionally distinct, we generated PPARγ1 and PPARγ2 tagged mice using CRISPR-Cas9 technology. PPARγ1 and PPARγ2 specific knockout mice were also generated incidentally during this process, via aberrant recombination. By reverse-transcription quantitative PCR (RT-qPCR), and western blot, we confirmed the presence of the appropriate tags in our PPARγ1 and PPARγ2 tagged mice, with no significant disruption to mRNA or protein expression. Furthermore, we found that PPARγ1 mRNA and protein expression levels were reduced in our PPARγ1 knockout model, compared to the wild type. Interestingly, we found that there was a complete loss of PPARγ2 protein expression, despite an increase in PPARγ2 mRNA expression in our PPARγ2 knockout model. These data suggest that we have successfully generated PPARγ1 and PPARγ2 knockin and knockout mice. Our mouse models provide a valuable tool to study the individual roles of PPARγ1 and PPARγ2 in adipogenesis, insulin sensitivity and metabolic disease

Foreword

This work reports on the performances of ohmic contacts fabricated on highly p-type doped 4H-SiC epitaxial layer selectively grown by vapor-liquid-solid transport. Due to the very high doping level obtained, the contacts have an ohmic behavior even without any annealing process. Upon variation of annealing temperatures, it was shown that both 500 and 800 °C annealing temperature lead to a minimum value of the Specific Contact Resistance (SCR) down to 1.3×10−6 Ω⋅cm2. However, a large variation of the minimum SCR values has been observed (up to 4×10−4 Ω⋅cm2). Possible sources of this fluctuation have been also discussed in this paper

Methoden zur Analyse der vokalen Gestaltung populärer Musik

Although voice and singing play a crucial role in many genres of popular music, to date there are only few approaches to an in-depth exploration of vocal expression. The paper aims at presenting new ways for describing, analysing and visualizing several aspects of singing using computer-based tools. After outlining a theoretical framework for the study of voice and singing in popular music, some of those tools are introduced and exemplified by vocal recordings from various genres (blues, gospel music, country music, jazz). Firstly, pitch gliding (slurs, slides, bends, melismas) and vibrato are discussed referring to a computer-based visualization of pitch contour. Secondly, vocal timbre and phonation (e.g. vocal roughness) are explored and visualized using spectrograms

Critical temperature of superconductor/ferromagnet bilayers

Superconductor/ferromagnet bilayers are known to exhibit nontrivial dependence of the critical temperature T_c on the thickness d_f of the ferromagnetic layer. We develop a general method for investigation of T_c as a function of the bilayer's parameters. It is shown that interference of quasiparticles makes T_c(d_f) a nonmonotonic function. The results are in good agreement with experiment. Our method also applies to multilayered structures.Comment: 4 pages, 2 EPS figures; the style file jetpl.cls is included. Version 2: typos correcte

Particle-in-cell Simulations of the Parallel Proton Firehose Instability Influenced by the Electron Temperature Anisotropy in Solar Wind Conditions

In situ observations of the solar wind show a limited level of particle temperature anisotropy with respect to the interplanetary magnetic field direction. Kinetic electromagnetic instabilities are efficient to prevent the excessive growth of the anisotropy of particle velocity distribution functions. Among them, the firehose instabilities are often considered to prevent the increase of the parallel temperature and hence to shape the velocity distribution functions of electrons and protons in the solar wind. We present a nonlinear modeling of the parallel firehose instability, retaining a kinetic description for both the electrons and protons. One-dimensional (1D) fully kinetic particle-in-cell simulations using the energy conserving semi-implicit method (ECsim) are performed to clarify the role of the electron temperature anisotropy in the development of the parallel proton firehose instability. We found that in the presence of an electron temperature anisotropy, such that the temperature parallel to the background magnetic field is higher than the temperature in the perpendicular direction, the onset of the parallel proton firehose instability occurs earlier and its growth rate is faster. The enhanced wave fluctuations contribute to the particle scattering reducing the temperature anisotropy to a stable, nearly isotropic state. The simulation results compare well with linear theory. A test case of 1D simulations at oblique angles with respect to the magnetic field is also considered, as a first step to study the cumulative effect of protons and electrons on the full spectrum of instabilities

Robustness of the filamentation instability as shock mediator in arbitrarily oriented magnetic field

The filamentation instability (sometimes also referred to as "Weibel") is a key process in many astrophysical scenario. In the Fireball model for Gamma Ray Bursts, this instability is believed to mediate collisionless shock formation from the collision of two plasma shells. It has been known for long that a flow aligned magnetic field can completely cancel this instability. We show here that in the general case where there is an angle between the field and the flow, the filamentation instability can never be stabilized, regardless of the field strength. The presented model analyzes the stability of two symmetric counter-streaming cold electron/proton plasma shells. Relativistic effects are accounted for, and various exact analytical results are derived. This result guarantees the occurrence of the instability in realistic settings fulfilling the cold approximation.Comment: To appear in Physics of Plasmas Letter

A system in balance? ? Implications of deep vertical mixing for the nitrogen budget in the northern Red Sea, including the Gulf of Aqaba (Eilat)

International audienceWe investigated the implications of deep winter mixing for the nitrogen budget in two adjacent systems, the northern Red Sea proper, and the Gulf of Aqaba. Both are subtropical oligotrophic water bodies. The main difference is that in the gulf deep winter mixing takes place regularly, whereas the northern Red Sea proper is permanently stratified. In the Gulf of Aqaba, we observed significantly lower nitrate deficits, i.e. deviations from the Redfield ratio, than in the northern Red Sea proper. Assuming that other external inputs and losses in N or P are very similar in both systems, the higher nitrate deficit can be explained by either lower nitrogen fixation in the (stratified) northern Red Sea, which seems unlikely. An alternative explanation would be higher rates of benthic denitrification than in the gulf. By comparing the two systems we have indirect evidence that benthic denitrification was much lower in the Gulf of Aqaba due to higher oxygen concentrations. This we attributed to the occurrence of deep winter mixing, and as a consequence, the nitrate deficit was close to zero (i.e. N:P ratio close to "Redfield"). If both nitrogen fixation and benthic denitrification take place, as in the northern Red Sea proper, the result was a positive nitrate deficit (i.e. a deficit in nitrate) in the ambient water. The nitrate deficit in the northern Red Sea was observed in spite of high iron deposition from the surrounding desert. Our results strongly support the concept of nitrogen as the proximate, and phosphate as the ultimate limiting nutrient for primary production in the sea. This must not be neglected in efforts for protecting the adjacent reefs against eutrophication