An assembly of nuclear bodies associates with the active VSG expression site in African trypanosomes

A Variant Surface Glycoprotein (VSG) coat protects bloodstream form Trypanosoma brucei. Prodigious amounts of VSG mRNA (~7-10% total) are generated from a single RNA polymerase I (Pol I) transcribed VSG expression site (ES), necessitating extremely high levels of localised splicing. We show that splicing is required for processive ES transcription, and describe novel ES-associated T. brucei nuclear bodies. In bloodstream form trypanosomes, the expression site body (ESB), spliced leader array body (SLAB), NUFIP body and Cajal bodies all frequently associate with the active ES. This assembly of nuclear bodies appears to facilitate the extraordinarily high levels of transcription and splicing at the active ES. In procyclic form trypanosomes, the NUFIP body and SLAB do not appear to interact with the Pol I transcribed procyclin locus. The congregation of a restricted number of nuclear bodies at a single active ES, provides an attractive mechanism for how monoallelic ES transcription is mediated

An enhancement to sea ice motion and age products at the National Snow and Ice Data Center (NSIDC)

A new version of sea ice motion and age products includes several significant upgrades in processing, corrects known issues with the previous version, and updates the time series through 2018, with regular updates planned for the future. First, we provide a history of these NASA products distributed at the National Snow and Ice Data Center. Then we discuss the improvements to the algorithms, provide validation results for the new (Version 4) and older versions, and intercompare the two. While Version 4 algorithm changes were significant, the impact on the products is relatively minor, particularly for more recent years. The changes in Version 4 reduce motion biases by &sim;&thinsp;0.01 to 0.02&thinsp;cm&thinsp;s&minus;1 and error standard deviations by &sim;&thinsp;0.3&thinsp;cm&thinsp;s&minus;1. Overall, ice speed increased in Version 4 over Version 3 by 0.5 to 2.0&thinsp;cm&thinsp;s&minus;1 over most of the time series. Version 4 shows a higher positive trend for the Arctic of 0.21&thinsp;cm&thinsp;s&minus;1 per decade compared to 0.13&thinsp;cm&thinsp;s&minus;1 per decade for Version 3. The new version of ice age estimates indicates more older ice than Version 3, especially earlier in the record, but similar trends toward less multiyear ice. Changes in sea ice motion and age derived from the product show a significant shift in the Arctic ice cover, from a pack with a high concentration of older ice to a sea ice cover dominated by first-year ice, which is more susceptible to summer melt. We also observe an increase in the speed of the ice over the time series &ge;&thinsp;30 years, which has been shown in other studies and is anticipated with the annual decrease in sea ice extent. </div

Air Corrosivity in U.S. Outdoor-Air-Cooled Data Centers is Similar to That in Conventional Data Centers

There is a concern that environmental-contamination caused corrosion may negatively affect Information Technology (IT) equipment reliability. Nineteen data centers in the United States and two in India were evaluated using Corrosion Classification Coupons (CCC) to assess environmental air quality as it may relate IT equipment reliability. The data centers were of two basic types: closed and outside-air cooled. A closed data center provides cool air to the IT equipment using air conditioning in which only a small percent age of the recirculation air is make-up air continuously supplied from outside to meet human health requirements. An outside-air cooled data center uses outside air directly as the primary source for IT equipment cooling. Corrosion measuring coupons containing copper and silver metal strips were placed in both closed and outside-air cooled data centers. The coupons were placed at each data center (closed and outside-air cooled types) with the location categorized into three groups: (1) Outside - coupons sheltered, located near or at the supply air inlet, but located before any filtering, (2) Supply - starting just after initial air filtering continuing inside the plenums and ducts feeding the data center rooms, and (3) Inside located inside the data center rooms near the IT equipment. Each coupon was exposed for thirty days and then sent to a laboratory for a corrosion rate measurement analysis. The goal of this research was to investigate whether gaseous contamination is a concern for U.S. data center operators as it relates to the reliability of IT equipment. More specifically, should there be an increased concern if outside air for IT equipment cooling is used To begin to answer this question limited exploratory measurements of corrosion rates in operating data centers in various locations were undertaken. This study sought to answer the following questions: (1) What is the precision of the measurements (2) What are the approximate statistical distributions of copper and silver corrosion rates in the sampled data centers(3) To what extent are copper and silver corrosion measurements related (4) What is the relationship of corrosion rate measurements between outside-air cooled data centers compared to closed data centers (5) How do corrosivity measurements relate to IT equipment failure rates The data from our limited sample size suggests that most United States data center operators should not be concerned with environmental gaseous contamination causing high IT equipment failure rates even when using outside-air cooling. The research team recommends additional basic research on how environmental conditions, specifically gaseous contamination, affect electronic equipment reliability

Workgroup Report: Incorporating In Vitro Alternative Methods for Developmental Neurotoxicity into International Hazard and Risk Assessment Strategies

This is the report of the first workshop on Incorporating In Vitro Alternative Methods for Developmental Neurotoxicity (DNT) Testing into International Hazard and Risk Assessment Strategies, held in Ispra, Italy, on 19–21 April 2005. The workshop was hosted by the European Centre for the Validation of Alternative Methods (ECVAM) and jointly organized by ECVAM, the European Chemical Industry Council, and the Johns Hopkins University Center for Alternatives to Animal Testing. The primary aim of the workshop was to identify and catalog potential methods that could be used to assess how data from in vitro alternative methods could help to predict and identify DNT hazards. Working groups focused on two different aspects: a) details on the science available in the field of DNT, including discussions on the models available to capture the critical DNT mechanisms and processes, and b) policy and strategy aspects to assess the integration of alternative methods in a regulatory framework. This report summarizes these discussions and details the recommendations and priorities for future work

A major genetic locus in <i>Trypanosoma brucei</i> is a determinant of host pathology

The progression and variation of pathology during infections can be due to components from both host or pathogen, and/or the interaction between them. The influence of host genetic variation on disease pathology during infections with trypanosomes has been well studied in recent years, but the role of parasite genetic variation has not been extensively studied. We have shown that there is parasite strain-specific variation in the level of splenomegaly and hepatomegaly in infected mice and used a forward genetic approach to identify the parasite loci that determine this variation. This approach allowed us to dissect and identify the parasite loci that determine the complex phenotypes induced by infection. Using the available trypanosome genetic map, a major quantitative trait locus (QTL) was identified on T. brucei chromosome 3 (LOD = 7.2) that accounted for approximately two thirds of the variance observed in each of two correlated phenotypes, splenomegaly and hepatomegaly, in the infected mice (named &lt;i&gt;TbOrg1&lt;/i&gt;). In addition, a second locus was identified that contributed to splenomegaly, hepatomegaly and reticulocytosis (&lt;i&gt;TbOrg2&lt;/i&gt;). This is the first use of quantitative trait locus mapping in a diploid protozoan and shows that there are trypanosome genes that directly contribute to the progression of pathology during infections and, therefore, that parasite genetic variation can be a critical factor in disease outcome. The identification of parasite loci is a first step towards identifying the genes that are responsible for these important traits and shows the power of genetic analysis as a tool for dissecting complex quantitative phenotypic traits

ESPRESSO: The next European exoplanet hunter

The acronym ESPRESSO stems for Echelle SPectrograph for Rocky Exoplanets and Stable Spectroscopic Observations; this instrument will be the next VLT high resolution spectrograph. The spectrograph will be installed at the Combined-Coud\'e Laboratory of the VLT and linked to the four 8.2 m Unit Telescopes (UT) through four optical Coud\'e trains. ESPRESSO will combine efficiency and extreme spectroscopic precision. ESPRESSO is foreseen to achieve a gain of two magnitudes with respect to its predecessor HARPS, and to improve the instrumental radial-velocity precision to reach the 10 cm/s level. It can be operated either with a single UT or with up to four UTs, enabling an additional gain in the latter mode. The incoherent combination of four telescopes and the extreme precision requirements called for many innovative design solutions while ensuring the technical heritage of the successful HARPS experience. ESPRESSO will allow to explore new frontiers in most domains of astrophysics that require precision and sensitivity. The main scientific drivers are the search and characterization of rocky exoplanets in the habitable zone of quiet, nearby G to M-dwarfs and the analysis of the variability of fundamental physical constants. The project passed the final design review in May 2013 and entered the manufacturing phase. ESPRESSO will be installed at the Paranal Observatory in 2016 and its operation is planned to start by the end of the same year.Comment: 12 pages, figures included, accepted for publication in Astron. Nach

HD 142527: quantitative disk polarimetry with SPHERE

We present high-precision photometry and polarimetry for the protoplanetary disk around HD142527, with a focus on determining the light scattering parameters of the dust. We re-reduced polarimetric differential imaging data of HD142527 in the VBB (735 nm) and H-band (1625 nm) from the ZIMPOL and IRDIS subinstruments of SPHERE/VLT. With polarimetry and photometry based on reference star differential imaging, we were able to measure the linearly polarized intensity and the total intensity of the light scattered by the circumstellar disk with high precision. We used simple Monte Carlo simulations of multiple light scattering by the disk surface to derive constraints for three scattering parameters of the dust: the maximum polarization of $P_{\rm max}$, the asymmetry parameter $g$, and the single-scattering albedo $\omega$. We measure a reflected total intensity of $51.4\pm1.5$ mJy and $206\pm12$ mJy and a polarized intensity of $11.3\pm0.3$ mJy and $55.1\pm3.3$ mJy in the VBB and H-band, respectively. We also find in the visual range a degree of polarization that varies between $28\%$ on the far side of the disk and $17\%$ on the near side. The disk shows a red color for the scattered light intensity and the polarized intensity, which are about twice as high in the near-infrared when compared to the visual. We determine with model calculations the scattering properties of the dust particles and find evidence for strong forward scattering ($g\approx 0.5-0.75$), relatively low single-scattering albedo ($\omega \approx 0.2-0.5$), and high maximum polarization ($P_{\rm max} \approx 0.5-0.75$) at the surface on the far side of the disk for both observed wavelengths. The optical parameters indicate the presence of large aggregate dust particles, which are necessary to explain the high maximum polarization, the strong forward-scattering nature of the dust, and the observed red disk color.Comment: 20 pages, 14 figure

HARPS3 for a roboticized Isaac Newton telescope

This is the author accepted manuscript. The final version is available from the publisher via the DOI in this record.We present a description of a new instrument development, HARPS3, planned to be installed on an upgraded and roboticized Isaac Newton Telescope by end-2018. HARPS3 will be a high resolution (R = 115,000) echelle spectrograph with a wavelength range from 380-690 nm. It is being built as part of the Terra Hunting Experiment - a future 10 year radial velocity measurement programme to discover Earth-like exoplanets. The instrument design is based on the successful HARPS spectrograph on the 3.6m ESO telescope and HARPS-N on the TNG telescope. The main changes to the design in HARPS3 will be: a customised fibre adapter at the Cassegrain focus providing a stabilised beam feed and on-sky fibre diameter ~ 1.4 arcsec, the implementation of a new continuous flow cryostat to keep the CCD temperature very stable, detailed characterisation of the HARPS3 CCD to map the effective pixel positions and thus provide an improved accuracy wavelength solution, an optimised integrated polarimeter and the instrument integrated into a robotic operation. The robotic operation will optimise our programme which requires our target stars to be measured on a nightly basis. We present an overview of the entire project, including a description of our anticipated robotic operation.R.H. acknowledges the Science and Technologies Facilities Council (STFC) for his PhD studentship award (2015).J.I.G.H. acknowledges financial support from the Spanish Ministry of Economy and Competitiveness (MINECO) under the 2013 Ram´on y Cajal program MINECO RYC-2013-14875.J.I.G.H., R.R., and S.S.T. also acknowledge the Spanish ministry project MINECO AYA2014-56359-P.NP and ES are grateful to Knut and Alice Wallenberg Foundation for a generous support of the Swedish contribution to the THE project.AD acknowledges the support from Russian Foundation for Basic Research as part of research grant 15-52-12371