Enhancing high-intensity actions during a basketball game after a strength training program with random recovery times between sets

To examine the effects of a strength training program with random recovery times between sets in consideration of several physical parameters, high-intensity actions (HIA), and spatial exploration index during a simulated basketball game. Twenty male basketball players (age: 19.45 ± 4.36 years) were assigned randomly, either to strength training group (n = 10), or a control group (n = 10). The strength training included: parallel back squat and bench press exercises, twice a week for the duration of 10 weeks, with two blocks of 5 sets × 5 repetitions interspersed with variable passive recovery (range = 15–35 sec.) between sets, and constant passive recovery (3-min) between blocks with the load that maximized propulsive power output. The pre- and post-test assessments included jumping (bilateral and unilateral), change-of-direction, straight sprinting, and a 5-on-5 full-court situation. The external training load was assessed using the local positioning system, and the internal load was recorded with the use of individual heart rate monitors. A significant interaction effect (group x time) was observed on countermovement jump (CMJ), unilateral right hops, high-intensity accelerations and decelerations, and peak accelerations and decelerations in the 5-on-5 full-court situation. Relative improvements observed and recorded in the training group on unilateral right hops, accelerations, and decelerations were correlated. Similar results were observed on 0–25m sprints, high-intensity decelerations, peak accelerations, and decelerations. Strength training paired with random recovery times enhanced physical and game-related aspects in the observed basketball players

The Representation of Women’s Roles in Television Series in Spain. Analysis of the Basque and Catalan Cases

The comparison of six Spanish television dramas has enabled us to analyse the role of women in these fictional products. In the series analysed there is a persistence of males playing the leading roles, as well as a reiteration of female stereotypes. In the case of the Catalan television dramas on TVC (El cor de la ciutat, Ventdelplà and Infidels) a more positive treatment of femininity could be observed in the series dealing with social customs. In the Basque productions on EITB (Goenkale, Martin and Mi querido Klikowsky) there is a perpetuation of the cliché of the Basque woman as being strong and capable of managing her home and family, as well as of her feelings. ----------------------------------------------------- La comparación de seis dramas televisivos españoles nos ha permitido analizar la imagen de la mujer en estos productos de ficción. En las series analizadas se observa una persistencia de la imagen del hombre en los papeles principales, así como un mantenimiento de los estereotipos femeninos. En el caso de los programas catalanes de TVC (El cor de la ciutat, Ventdelplà e Infidels) constatamos un mejor tratamiento de la imagen de la mujer en los productos costumbristas. En los programas vascos de la EITB (Goenkale, Martin y Mi querido Klikowsky) observamos una perpetuación del cliché de la mujer vasca fuerte y capaz de gestionar su casa, su familia y sus sentimientos

t(6;20)(q13;q12) LMBRD1/CHD6

Short communication on t t(6;20)(q13;q12) LMBRD1/CHD6, with data on clinics, and the genes implicated

t(5;11)(q35;q12) NSD1/FEN1

Review on t(5;11)(q35;q12) NSD1/FEN1, with data on clinics, and the genes implicated

Comparison of a manual and an automated tracking method for Tibetan Plateau vortices

Tibetan Plateau vortices (TPVs) are mesoscale cyclones originating over the Tibetan Plateau (TP) during the extended summer season (April-September). Most TPVs stay on the TP while a small number can move off the TP to the east. TPVs are known to be one of the main precipitation-bearing systems on the TP and moving-off TPVs have been associated with heavy precipitation and flooding downstream of the TP (e.g. Sichuan province, Yangtze River Valley). Identifying and tracking TPVs is difficult both due to their comparatively small horizontal extent (400 – 800 km) and the limited availability of soundings over the TP, which, in turn, constitutes a challenge for short-term predictions of TPV-related impacts and for the climatological study of TPVs. In this study, (i) manual tracking (MT) results using radiosonde data from a network over and downstream of the TP are compared with (ii) results obtained by an automated tracking (AT) algorithm applied to ERA-Interim reanalysis. Ten MT-TPV cases are selected based on method (i) and matched to and compared with the corresponding AT-TPVs identified with method (ii). Conversely, ten AT-TPVs are selected and compared with the corresponding MT-TPVs. In general, the comparison shows good results in cases where the underlying data are in good agreement, but considerable differences are also seen in some cases and explained in terms of differences in the tracking methods, data availability/coverage and disagreement between sounding and ERA-Interim data. Recommendations are given for future efforts in TPV detection and tracking, including in an operational weather forecasting context

Covid-19: A Disaster in the Linguistic Dimension of Different Countries

The article was submitted on 25.09.2020.Covid-19 is highly relevant in 2020; among other things, it is attracting new global socio-communicative and linguistic research. Scholars are addressing the linguistic response to the social and psychological situation in different countries in the era of coronavirus. Thus, the Editorial Board has created a forum for specialists to communicate (in writing), one which makes it possible to provide information about their sources on Covid-19 and illustrate theoretical materials. The participants chose to analyse different aspects of language during the pandemic; medical terminology and its relevant vocabulary were the same for all countries. The conversation goes beyond the scope of linguistics, as it is important for the researchers to characterise measures taken by governments to combat Covid-19 and the public’s reaction to them as reflected through language. Additionally, the authors focus on spontaneous linguistic responses to the pandemic in the form of language games, metaphors, and references to historical memory of combatting disasters. The pandemic has also caused long-standing forms of speech communication to change. Researchers from different European countries have took part: Arto Mustajoki (University of Helsinki, Finland; National Research University Higher School of Economics, Moscow, Russia), Nadezjda Zorikhina Nilsson (Stockholm University, Sweden), Rafael Guzmán Tirado (University of Granada, Spain), Anna Tous-Rovirosa and Daria Dergacheva (Autonomous University of Barcelona, Spain). The conversation was moderated by Irina Vepreva and Tatiana Itskovich (Ural Federal University Yekaterinburg, Russia).Тема Covid-19, актуальная для этого года, обретает новые грани как исследовательская проблема мирового масштаба в социокоммуникативном и лингвистическом аспектах. Исследователи обращаются к проблеме язы-кового реагирования на социально-психологическую обстановку, сложившуюся в разных странах в эпоху коронавируса. Объективные обстоятельства современного общения заставляют научное сообщество искать новый формат обсуждения насущных проблем. Предложенная редколлегией журнала форма письменного диалога позволяет как привести информацию об источниках высказываний о коронавирусе, так и подкрепить теоретические положения ссылками на научные изыскания. Участники дискуссии выбрали различные аспекты анализа языкового существования в период пандемии. В поле наблюдений были включены вопросы, связанные с лексиконом дискурса пандемии, который носит глобальный характер: медицинская терминология и актуальная лексика оказались общими для всех стран. Разговор выходит за рамки лингвистического обзора, поскольку важно было охарактеризовать действия правительства по борьбе с коронавирусом и общественную реакцию на эти действия, отраженную в языковых явлениях. В стороне не осталось обсуждение стихийно складывающейся языковой реакции на пандемию, выраженной в фактах языковой игры и метафорики, а также обращения к исторической памяти народа о противостоянии бедствиям. Пандемия оказала влияние и на устоявшиеся формы речевой коммуникации. В обсуждении приняли участие исследователи из разных стран Европы: Арто Мустайоки (А. М.; Хельсинкский университет, Финляндия; Национальный исследовательский университет «Высшая школа экономики», Москва, Россия )1, Надежда Зорихина-Нильссон (Н. З.-Н.; Стокгольмский университет, Швеция), Рафаэль Гусман Тирадо (Р. Г.-Т.; Гранадский университет, Испания), Анна Тоус-Ровироса, Дарья Дергачева (А. Т.-Р., Д. Д.; Свободный университет Барселоны, Испания). Модераторами обсуждения стали Ирина Вепрева и Татьяна Ицкович (И. В., Т. И.; Уральский федеральный университет, Екатеринбург, Росси я)2

Involvement in surface antigen expression by a moonlighting FG-repeat nucleoporin in trypanosomes

Components of the nuclear periphery coordinate a multitude of activities, including macromolecular transport, cell-cycle progression, and chromatin organization. Nuclear pore complexes (NPCs) mediate nucleocytoplasmic transport, mRNA processing, and transcriptional regulation, and NPC components can define regions of high transcriptional activity in some organisms at the nuclear periphery and nucleoplasm. Lineage-specific features underpin several core nuclear functions and in trypanosomatids, which branched very early from other eukaryotes, unique protein components constitute the lamina, kinetochores, and parts of the NPCs. Here we describe a phenylalanine-glycine (FG)-repeat nucleoporin, TbNup53b, that has dual localizations within the nucleoplasm and NPC. In addition to association with nucleoporins, TbNup53b interacts with a known trans-splicing component, TSR1, and has a role in controlling expression of surface proteins including the nucleolar periphery-located, procyclin genes. Significantly, while several nucleoporins are implicated in intranuclear transcriptional regulation in metazoa, TbNup53b appears orthologous to components of the yeast/human Nup49/Nup58 complex, for which no transcriptional functions are known. These data suggest that FG-Nups are frequently co-opted to transcriptional functions during evolution and extend the presence of FG-repeat nucleoporin control of gene expression to trypanosomes, suggesting that this is a widespread and ancient eukaryotic feature, as well as underscoring once more flexibility within nucleoporin function

Proteomic identification and characterization of hepatic glyoxalase 1 dysregulation in non-alcoholic fatty liver disease

Background: Non-alcoholic fatty liver disease (NAFLD) is the most common liver disease worldwide. However, its molecular pathogenesis is incompletely characterized and clinical biomarkers remain scarce. The aims of these experiments were to identify and characterize liver protein alterations in an animal model of early, diet-related, liver injury and to assess novel candidate biomarkers in NAFLD patients. Methods: Liver membrane and cytosolic protein fractions from high fat fed apolipoprotein E knockout (ApoE−/−) animals were analyzed by quantitative proteomics, utilizing isobaric tags for relative and absolute quantitation (iTRAQ) combined with nano-liquid chromatography and tandem mass spectrometry (nLC-MS/MS). Differential protein expression was confirmed independently by immunoblotting and immunohistochemistry in both murine tissue and biopsies from paediatric NAFLD patients. Candidate biomarkers were analyzed by enzyme-linked immunosorbent assay in serum from adult NAFLD patients. Results: Through proteomic profiling, we identified decreased expression of hepatic glyoxalase 1 (GLO1) in a murine model. GLO1 protein expression was also found altered in tissue biopsies from paediatric NAFLD patients. In vitro experiments demonstrated that, in response to lipid loading in hepatocytes, GLO1 is first hyperacetylated then ubiquitinated and degraded, leading to an increase in reactive methylglyoxal. In a cohort of 59 biopsy-confirmed adult NAFLD patients, increased serum levels of the primary methylglyoxal-derived advanced glycation endproduct, hydroimidazolone (MG-H1) were significantly correlated with body mass index (r = 0.520, p < 0.0001). Conclusion: Collectively these results demonstrate the dysregulation of GLO1 in NAFLD and implicate the acetylation-ubquitination degradation pathway as the functional mechanism. Further investigation of the role of GLO1 in the molecular pathogenesis of NAFLD is warranted. Keywords: Non-alcoholic fatty liver disease, Glyoxalase, Methylglyoxal, Proteomics, iTRA

SAM levels, gene expression of SAM synthetase, methionine synthase and ACC oxidase, and ethylene emission from N. suaveolens flowers

S′adenosyl-l-methionine (SAM) is a ubiquitous methyl donor and a precursor in the biosynthesis of ethylene, polyamines, biotin, and nicotianamine in plants. Only limited information is available regarding its synthesis (SAM cycle) and its concentrations in plant tissues. The SAM concentrations in flowers of Nicotiana suaveolens were determined during day/night cycles and found to fluctuate rhythmically between 10 and 50 nmol g−1 fresh weight. Troughs of SAM levels were measured in the evening and night, which corresponds to the time when the major floral scent compound, methyl benzoate, is synthesized by a SAM dependent methyltransferase (NsBSMT) and when this enzyme possesses its highest activity. The SAM synthetase (NsSAMS1) and methionine synthase (NsMS1) are enzymes, among others, which are involved in the synthesis and regeneration of SAM. Respective genes were isolated from a N. suaveolens petal cDNA library. Transcript accumulation patterns of both SAM regenerating enzymes matched perfectly those of the bifunctional NsBSMT; maximum mRNA accumulations of NsMS1 and NsSAMS1 were attained in the evening. Ethylene, which is synthesized from SAM, reached only low levels of 1–2 ppbv in N. suaveolens flowers. It is emitted in a burst at the end of the life span of the flowers, which correlates with the increased expression of the 1-aminocyclopropane-1-carboxylate oxidase (NsACO)

High-Throughput Sequencing of RNA Silencing-Associated Small RNAs in Olive (Olea europaea L.)

Small RNAs (sRNAs) of 20 to 25 nucleotides (nt) in length maintain genome integrity and control gene expression in a multitude of developmental and physiological processes. Despite RNA silencing has been primarily studied in model plants, the advent of high-throughput sequencing technologies has enabled profiling of the sRNA component of more than 40 plant species. Here, we used deep sequencing and molecular methods to report the first inventory of sRNAs in olive (Olea europaea L.). sRNA libraries prepared from juvenile and adult shoots revealed that the 24-nt class dominates the sRNA transcriptome and atypically accumulates to levels never seen in other plant species, suggesting an active role of heterochromatin silencing in the maintenance and integrity of its large genome. A total of 18 known miRNA families were identified in the libraries. Also, 5 other sRNAs derived from potential hairpin-like precursors remain as plausible miRNA candidates. RNA blots confirmed miRNA expression and suggested tissue- and/or developmental-specific expression patterns. Target mRNAs of conserved miRNAs were computationally predicted among the olive cDNA collection and experimentally validated through endonucleolytic cleavage assays. Finally, we use expression data to uncover genetic components of the miR156, miR172 and miR390/TAS3-derived trans-acting small interfering RNA (tasiRNA) regulatory nodes, suggesting that these interactive networks controlling developmental transitions are fully operational in olive