24 research outputs found
Using of advanced IT technologies and innovayive concepts of modern project management at preparing a newgeneration of managers
CsMgPO4
Caesium magnesium orthophosphate is built up from MgO4 and PO4 tetraĀhedra (both with . m. symmetry) linked together by corners, forming a three-dimensional framework. The Cs atoms have .m. site symmetry and are located in hexaĀgonal channels running along the a- and b-axis directions
The Theoretical Description for Chlorantraniliprole Electrochemical Determination, Assisted by Squaraine Dye ā Nano-CuS Composite
The theoretical description for the chlorantraniliprole electrochemical determination, assisted by the hybrid composite of squaraine dye with CuS nanoparticles has been described. The correspondent reaction mechanism has been proposed, and the correspondent mathematical model has been developed and analyzed by means of linear stability theory and bifurcation analysis. It has been shown that the chlorantraniprole electrochemical anodical determination on high potential may be efficiently provided by cupper sulfide nanoparticles, stabilized by the squaraine dye. On the other hand, the oscillatory and monotonic instability is also possible, being caused by DEL influences of the electrochemical stage.
DOI: http://dx.doi.org/10.17807/orbital.v13i3.151
Theoretical Description for Omeprazole Cathodical Electrochemical Determination, Assisted by Omeprazole Electrochemical Determination, Assisted by the Composite Poly(1,2,4-triazole) ā VO(OH)
Abstract: In this work, we describe theoretically the possibility of omeprazole electrochemical determination, assisted by the composite containing vanadium oxyhydroxide as an active substance and the polymer of 1,2,4-triazolic derivative as a mediator. The omeprazole molecule undergoes a sulfoxide to sulfide reduction process over a trivalent vanadium compound. The vanadium oxyhydroxide, at its turn, may be oxidized to a tetravalent state, represented in two forms. The electroanalytical process behavior will be illustrated by a trivariate equation-set, analysis of which confirms the efficiency of the composite of poly (1,2,4-triazole) with VO(OH). Vanadium (III) oxyhydroxide may be efficiently used for omeprazole detection both in pharmaceutical formulations, food, and biological liquids
A new class of glycomimetic drugs to prevent free fatty acid-induced endothelial dysfunction
Background:Ā CarbohydratesĀ playĀ aĀ majorĀ roleĀ inĀ cellĀ signalingĀ inĀ manyĀ biologicalĀ processes.Ā WeĀ have developedĀ aĀ setĀ ofĀ glycomimeticĀ drugsĀ thatĀ mimicĀ theĀ structureĀ ofĀ carbohydratesĀ andĀ representĀ aĀ novelĀ source ofĀ therapeuticsĀ forĀ endothelialĀ dysfunction,Ā aĀ keyĀ initiatingĀ factorĀ inĀ cardiovascularĀ complications. Purpose:Ā OurĀ objectiveĀ wasĀ toĀ determineĀ theĀ protectiveĀ effectsĀ ofĀ smallĀ moleculeĀ glycomimeticsĀ againstĀ free fattyĀ acidĀinducedĀ endothelialĀ dysfunction,Ā focusingĀ onĀ nitricĀ oxideĀ (NO)Ā andĀ oxidativeĀ stressĀ pathways. Methods:Ā FourĀ glycomimeticsĀ wereĀ synthesizedĀ byĀ theĀ stepwiseĀ transformationĀ ofĀ 2,5ĀdihydroxybenzoicĀ acidĀ to aĀ rangeĀ ofĀ 2,5ĀsubstitutedĀ benzoicĀ acidĀ derivatives,Ā incorporatingĀ theĀ keyĀ sulfateĀ groupsĀ toĀ mimicĀ the interactionsĀ ofĀ heparanĀ sulfate.Ā EndothelialĀ functionĀ wasĀ assessedĀ usingĀ acetylcholineĀinduced,Ā endotheliumdependent relaxationĀ inĀ mouseĀ thoracicĀ aorticĀ ringsĀ usingĀ wireĀ myography.Ā HumanĀ umbilicalĀ veinĀ endothelialĀ cell (HUVEC)Ā behaviorĀ wasĀ evaluatedĀ inĀ theĀ presenceĀ orĀ absenceĀ ofĀ theĀ freeĀ fattyĀ acid,Ā palmitate,Ā withĀ orĀ without glycomimeticsĀ (1ĀµM).Ā DAFĀ2Ā andĀ H2DCFĀDAĀ assaysĀ wereĀ usedĀ toĀ determineĀ nitricĀ oxideĀ (NO)Ā andĀ reactive oxygenĀ speciesĀ (ROS)Ā production,Ā respectively.Ā LipidĀ peroxidationĀ colorimetricĀ andĀ antioxidantĀ enzymeĀ activity assaysĀ wereĀ alsoĀ carriedĀ out.Ā RTĀPCRĀ andĀ westernĀ blottingĀ wereĀ utilizedĀ toĀ measureĀ Akt,Ā eNOS,Ā NrfĀ2, NQOĀ1Ā andĀ HOĀ1Ā expression. Results:Ā ExĀ vivoĀ endotheliumĀdependentĀ relaxationĀ wasĀ significantlyĀ improvedĀ byĀ theĀ glycomimeticsĀ under palmitateĀinducedĀ oxidativeĀ stress.Ā InĀ vitroĀ studiesĀ showedĀ thatĀ theĀ glycomimeticsĀ protectedĀ HUVECsĀ against theĀ palmitateĀinducedĀ oxidativeĀ stressĀ andĀ enhancedĀ NOĀ production.Ā WeĀ demonstrateĀ thatĀ theĀ protective effectsĀ ofĀ preĀincubationĀ withĀ glycomimeticsĀ occurredĀ viaĀ upregulationĀ ofĀ Akt/eNOSĀ signaling,Ā activationĀ ofĀ the Nrf2/AREĀ pathway,Ā andĀ suppressionĀ ofĀ ROSĀinducedĀ lipidĀ peroxidation. Conclusion:Ā WeĀ haveĀ developedĀ aĀ novelĀ setĀ ofĀ smallĀ moleculeĀ glycomimeticsĀ thatĀ protectĀ againstĀ freeĀ fattyĀ acidinduced endothelialĀ dysfunctionĀ andĀ thus,Ā representĀ aĀ newĀ categoryĀ ofĀ therapeuticĀ drugsĀ toĀ targetĀ endothelial damage,Ā theĀ firstĀ lineĀ ofĀ defenseĀ againstĀ cardiovascularĀ disease
COMPETENCIES APPROACH OF TEACHING INFORMATION SYSTEMS AND TECHNOLOGY OF THE FUTURE TEACHERS OF ECONOMICS
The Article is dedicated to possibility of the use competencies approach in process of the education information system and technology of the future teachers of the economics. It is thus suggested to use an educational information environment that includes the distance course 'Information systems and technology in the economy' and pedagogical software program 'Financial analysis and optimization' that has been designed by the authors of teaching information systems and technologies to future teachers of economics. During work in such educational information environment, students of economics specialities of pedagogical universities not only acquire competencies of the education information systems and technology in the economy but also professionally pedagogical competencies
The alternative approach to the preparation of complex calcium phosphates and their characterization
In this paper a new approach to the preparation of complex calcium-containing phosphates using molten chlorides and nitrates of alkaline metals as fluxes was described. The main advantages of used technique for synthesis of double phosphates comparing with traditional methods (solid state reactions or crystallization of a high temperature self-flux) were discussed. It was found that the phase composition of obtained crystalline products depends on type of salt melts, nature of alkaline metals and type of initial components. Formation conditions for compounds MICaPOā, MIāCaPāOā (MI - Na, K), CaāPāOā, CaāPOāCl and Caāā(POā)āClā were established. The synthesized phosphates were characterized using powder X-ray diffraction, Fourier transform infrared spectroscopy and optical microscopy
Stimulation of the endogenous hydrogen sulfide synthesis suppresses oxidative-nitrosative stress and restores endothelial-dependent vasorelaxation in old rats
Hydrogen sulfide (H2S) is an endogenous gas transmitter with profound effects on the cardiovascular system. We hypothesized that stimulation of H2S synthesis might alleviate age-associated changes in vascular reactivity. Pyridoxal-5-phosphate (PLP), the coenzyme of H2S-synthesizing enzymes, was administrated to old male Wistar rats per os at a dose of 0.7 mg/kg body mass once a day for 2 weeks. H2S content in the aortic tissue, markers of oxidative stress, inducible nitric oxide synthase (iNOS) and constitutive nitric oxide synthase (cNOS), arginase activities, and endothelium-dependent vasorelaxation of the aortic rings were studied. Our results showed that PLP restored endogenous H2S and low molecular weight S-nitrosothiol levels in old rat aorta to the levels detected in adults. PLP significantly reduced diene conjugate content, hydrogen peroxide and peroxynitrite generation rates, and iNOS and arginase activity in the aortic tissue of old rats. PLP also greatly improved acetylcholine-induced relaxation of old rat aorta (47.7% Ā± 4.8% versus 18.4% Ā± 4.1% in old rats, P < 0.05) that was abolished by NO inhibition with N-nitro-l-arginine methyl ester hydrochloride (L-NAME) or H2S inhibition with O-carboxymethylhydroxylamine (O-CMH). Thus, PLP might be used for stimulation of endogenous H2S synthesis and correction of oxidative and nitrosative stress and vessel tone dysfunction in aging and age-associated diseases.The accepted manuscript in pdf format is listed with the files at the bottom of this page. The presentation of the authors' names and (or) special characters in the title of the manuscript may differ slightly between what is listed on this page and what is listed in the pdf file of the accepted manuscript; that in the pdf file of the accepted manuscript is what was submitted by the author