Occurrence of dusky sweeper Pempheris adusta Bleeker, 1877 in Ratnagiri waters, Maharashtra

Pempheris adusta Bleeker, 1877, commonly called ‘dusky sweeper’ (Fig. 1), generally live on coral reefs and form schools in large caves among rocks. On 19/08/2009, a good number of specimens of P. adusta were observed at Mirkarwada landing centre in Ratnagiri (Fig. 2). They had elliptical, compressed body with iridescent, coppery silver colouration, because of which they were spectacular in appearance

Concept of Greeshma Rutucharya w.s.r. to Sarvanga Sundara and Ayurveda Rasayana Teekas of Ashtang Hrudayam

Health is the supreme foundation for the achievement of happy life, objective of Ayurveda is to accomplish the physical, mental, social and spiritual well being by adopting the preventive and promotive approach as well as treating the disease with its various remissive approaches. Ayurveda has given first priorty in maintaining the health of the individuals rather than treating diseases. It deals with different regimens maintain person’s health one of them is Rutucharya. Not following proper Rutucharya provoke the Doshas in turn causes diseases. Thus proper and thorough study of Rutucharya helps to adapt and prevent helath problems majorly those occurs in Greeshma Rutu (Summer Season). Acharya Vagbhata, the last among Brahattrayi collected many points from Charaka Samhita and Sushruta Samhita. So it is better to refer Ashtang Hrudayam is upgraded from among these Samhitas. So commentators of Ashtang Hrudayam, Sarvanga Sundara and Ayurveda Rasayana are studied regarding Greeshma Rutucharya

An overview on concept of Jatharagni and its role in Amotpatti

Agni in Ayurveda has a significant role to maintain body homeostasis, body functioning, metabolism of body and proper functioning of body. Agni in Ayurveda is correlated with digestive power of the body. There are many reasons behind the maintenance of Agni in Ayurveda. The main reason behind its maintenance is that it helps in growth and development of the body. Agni is innumerable because of its presence in each and every Paramanu of the body, according to the functions and site of action Agni has been divided in three types those are Jhataragni (digestive fire), Bhutagni and Dhatwagni. They work together to maintain the balance in the body as Jhatragni is maintaining metabolism. Imbalance is the physiology of Agni is the main cause for the formation of Ama which is the main reason for many diseases. It is also widely thought that if Agni is strong, then one is free from disease. So Jatharagni is important for every function of body. This article shows the importance of Jatharagni and its role in Amotpatti

Designer quantum spin Hall phase transition in molecular graphene

Graphene was the first material predicted to be a time-reversal-invariant topological insulator; however, the insulating gap is immeasurably small owing to the weakness of spin-orbit interactions in graphene. A recent experiment [1] demonstrated that designer honeycomb lattices with graphene-like "Dirac" band structures can be engineered by depositing a regular array of carbon monoxide atoms on a metallic substrate. Here, we argue that by growing such designer lattices on metals or semiconductors with strong spin-orbit interactions, one can realize an analog of graphene with strong intrinsic spin-orbit coupling, and hence a highly controllable two-dimensional topological insulator. We estimate the range of substrate parameters for which the topological phase is achievable, and consider the experimental feasibility of some candidate substrates.Comment: 5 pages, 3 figure

An optimised step-by-step protocol for measuring relative telomere length

Telomeres represent the nucleotide repeat sequences at the ends of chromosomes and are essential for chromosome stability. They can shorten at each round of DNA replication mainly because of incomplete DNA synthesis of the lagging strand. Reduced relative telomere length is associated with aging and a range of disease states. Different methods such as terminal restriction fragment analysis, real-time quantitative PCR (qPCR) and fluorescence in situ hybridization are available to measure telomere length; however, the qPCR-based method is commonly used for large population-based studies. There are multiple variations across qPCR-based methods, including the choice of the single-copy gene, primer sequences, reagents, and data analysis methods in the different reported studies so far. Here, we provide a detailed step-by-step protocol that we have optimized and successfully tested in the hands of other users. This protocol will help researchers interested in measuring relative telomere lengths in cells or across larger clinical cohort/study samples to determine associations of telomere length with health and disease

Synthesis and antiinflammatory activity of some 3-(2-thiazolyl)-1 ,2-benzisothiazoles

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Epigenetic and transcriptome profiling identifies a population of visceral adipose-derived progenitor cells with potential to differentiate into an endocrine pancreatic lineage

Type 1 diabetes (T1D) is characterized by the loss of insulin-producing β-cells in the pancreas. T1D can be treated using cadaveric islet transplantation, but this therapy is severely limited by a lack of pancreas donors. To develop an alternative cell source for transplantation therapy, we carried out the epigenetic characterization in nine different adult mouse tissues and identified visceral adipose-derived progenitors as a candidate cell population. Chromatin conformation, assessed using chromatin immunoprecipitation (ChIP) sequencing and validated by ChIP-polymerase chain reaction (PCR) at key endocrine pancreatic gene promoters, revealed similarities between visceral fat and endocrine pancreas. Multiple techniques involving quantitative PCR, in-situ PCR, confocal microscopy, and flow cytometry confirmed the presence of measurable (2–1000-fold over detectable limits) pancreatic gene transcripts and mesenchymal progenitor cell markers (CD73, CD90 and CD105; >98%) in visceral adipose tissue-derived mesenchymal cells (AMCs). The differentiation potential of AMCs was explored in transgenic reporter mice expressing green fluorescent protein (GFP) under the regulation of the Pdx1 (pancreatic and duodenal homeobox-1) gene promoter. GFP expression was measured as an index of Pdx1 promoter activity to optimize culture conditions for endocrine pancreatic differentiation. Differentiated AMCs demonstrated their capacity to induce pancreatic endocrine genes as evidenced by increased GFP expression and validated using TaqMan real-time PCR (at least 2–200-fold relative to undifferentiated AMCs). Human AMCs differentiated using optimized protocols continued to produce insulin following transplantation in NOD/SCID mice. Our studies provide a systematic analysis of potential islet progenitor populations using genome-wide profiling studies and characterize visceral adipose-derived cells for replacement therapy in diabetes

A Protocol for Measurement of Noncoding RNA in Human Serum

MicroRNAs (miRNAs) are small noncoding RNAs that act as regulators of gene expression by targeting mature messenger RNAs. Following the initial report of the presence of miRNAs in serum and plasma a number of studies have successfully demonstrated the use of these miRNAs as biomarkers of disease. Currently, there are many methods of isolating total RNA from liquid samples. Here, we describe a simple, cost effective method for extraction of RNA from human serum as well as subsequent real time PCR analysis of miRNA levels