A novel programmable lysozyme-based lysis system in Pseudomonas putida for biopolymer production

Indexación: Scopus; Web of Science.Cell lysis is crucial for the microbial production of industrial fatty acids, proteins, biofuels, and biopolymers. In this work, we developed a novel programmable lysis system based on the heterologous expression of lysozyme. The inducible lytic system was tested in two Gram-negative bacterial strains, namely Escherichia coli and Pseudomonas putida KT2440. Before induction, the lytic system did not significantly arrest essential physiological parameters in the recombinant E. coli (ECPi) and P. putida (JBOi) strain such as specific growth rate and biomass yield under standard growth conditions. A different scenario was observed in the recombinant JBOi strain when subjected to PHA-producing conditions, where biomass production was reduced by 25% but the mcl-PHA content was maintained at about 30% of the cell dry weight. Importantly, the genetic construct worked well under PHA-producing conditions (nitrogen-limiting phase), where more than 95% of the cell population presented membrane disruption 16 h post induction, with 75% of the total synthesized biopolymer recovered at the end of the fermentation period. In conclusion, this new lysis system circumvents traditional, costly mechanical and enzymatic cell-disrupting procedures.https://www.nature.com/articles/s41598-017-04741-2.pd

Geomorfología y paisaje en el ordenamiento territorial: valorizando el corredor inferior del río Mapocho

Considering the roles how biological corridor of the lower hydrological system of the Mapocho River, pointing to their integration in the urban planning of the respective communes from an ecologic, environmental and social perspective, is carried on a research about their geomorphological and landscape characteristics and the processes that are leading the evolution of the landscape. With this knowledge and from an ecosystemic perspective of the river territory, the limits of different homogeneous units were determined. Any geomorphological unit recognized, meaning also landscape systems, was characterized by its geology, sedimentology, soils and drainage, hydro-morphodynamic processes and human intervention, which allows through the determination of the quality and fragility of the geomorphic-landscape units, to establish land use proposals with their corresponding scopes on qualifications and restrictions. The resulting mapping contains implicit a proposal for land-use planning from the ecological planning point of view.    Considerando las funciones de corredor biológico del sistema hidrológico inferior del Río Mapocho, y apuntando a su integración a la planificación urbana de la intercomuna respectiva desde un punto de vista ecológico-ambiental y social, se reconocen sus características geomorfológicas y paisajísticas, y se establecen los procesos que lideran la evolución del modelado de las formas naturales. Con esta base se delimita el territorio fluvial desde una perspectiva ecosistémica y se determinan los límites de las diferentes unidades homogéneas resultantes. Cada unidad geomorfológica, en alta correspondencia con el sistema de paisajes, es caracterizada en función de su geología, sedimentología, suelos y drenaje, hidro-morfodinámica e intervención humana, lo cual permite mediante la determinación de la calidad y fragilidad de ellas, establecer propuestas de uso del suelo con sus correspondientes alcances sobre aptitudes y restricciones. La cartografía resultante contiene implícita una propuesta de ordenamiento del territorio desde el punto de vista de la planificación ecológica.  

Adoption of the personas technique in the open source software development process

The growth in the number of non-developer open source software (OSS) application users and the escalating use of these applications have led to the need and interest in developing usable OSS. OSS communities do not generally know how to apply usability techniques and are unclear about which techniques to use in each activity of the development process. The aim of our research is to adopt the Personas usability technique in the PSeInt OSS project and determine the feasibility of adapting the technique for application. To do this, we participated as volunteers in the project. We used the case study research method during technique application and participation in the community. As a result, we identified adverse conditions that were an obstacle to technique application and modified the technique to make it applicable. We can conclude from our experience that these changes were helpful for applying the technique, although it was not easy to recruit OSS users to participate in usability technique applicationThis research has been partly funded by several organizations, including the Government of Ecuador’s Secretariat of Higher Education, Science, Technology and Innovation (SENESCYT) through a scholarship and the State Technical University of Quevedo through doctoral training scholarships for university professors. Also this research was funded by the Spanish Ministry of Education, Culture and Sports FLEXOR and “Realizando Experimentos en la Industria del Software: Comprensión del Paso de Laboratorio a la Realidad” projects (TIN2014-52129-R and TIN2014-60490-P, respectively) and the eMadrid-CM “Investi-gación y Desarrollo de Tecnologías Educativas en la Comunidad de Madrid” project (S2013/ICE-2715

Forest growth dynamics of managed forests in the Southwestern Brazilian Amazon.

The objective of this work was to present the growth forest dynamics results obtained in two sites located in Acre and Amazonas states in the southwestern Brazilian Amazon.Abstracts of the XXV IUFRO World Congress

Formación de equipos en función de la extroversión de sus integrantes: un experimento replicado

Durante los cursos académicos 2010/2011 y 2011/2012 se han llevado a cabo un experimento y una réplica del mismo en el marco de la asignatura de bases de datos. La característica más destacable de estos estudios radica en que los equipos se han formado en función de un factor de personalidad de los miembros de los mismos, en concreto la extroversión. El objetivo de ambos estudios empíricos consiste en comprobar si el grado de extroversión del equipo (en función de la extroversión de los miembros del mismo) afecta, por un lado, a la calidad final de los productos software desarrollados y, por otro lado, a la satisfacción de los miembros del equipo durante el desarrollo de dichos productos. En este trabajo se presentan los resultados obtenidos en la réplica y la agregación de los resultados de ambos estudios. Como principal conclusión, cabe destacar que tanto la réplica como la agregación de resultados afianzan los resultados del experimento original, que establecían que a la hora de formar equipos, si el docente equilibra el número de estudiantes con carácter extrovertido y no extrovertido, conseguirá un alto nivel de satisfacción durante la realización de los proyectos sin que la calidad de los productos software desarrollados se vea mermada.A controlled experiment and a replication of it have been carried out during the academic courses 2010/2011 and 2011/2012 in the context of a databases subject. The most remarkable characteristic of these studies relies on the fact that the work-teams were set according to a personality factor of their members (extroversion). The goal of the studies is to check whether the extroversion degree of the teams (accordingly to the extroversion degree of their members) affects, on one hand, the global quality of the software products developed and, on the other hand, the satisfaction perceived by the members of the teams while developing those products. In this work, we present the results of the replication and the aggregation of both studies. As a main conclusion, all these results strengthen the conclusion obtained in the original controlled experiment. This conclusion states that balancing the number of extroverted and nonextroverted students in a team-work, makes the overall satisfaction level achieved to be the highest while the quality of the software products developed is not reduced

Are team personality and climate related to satisfaction and software quality? Aggregating results from a twice replicated experiment

This is the author’s version of a work that was accepted for publication in Information and Software Technology. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Information and Software Technology, [VOL 57, (2015)] DOI 10.1016/j.infsof.2014.09.002Context Research into software engineering teams focuses on human and social team factors. Social psychology deals with the study of team formation and has found that personality factors and group processes such as team climate are related to team effectiveness. However, there are only a handful of empirical studies dealing with personality and team climate and their relationship to software development team effectiveness. Objective We present aggregate results of a twice replicated quasi-experiment that evaluates the relationships between personality, team climate, product quality and satisfaction in software development teams. Method Our experimental study measures the personalities of team members based on the Big Five personality traits (openness, conscientiousness, extraversion, agreeableness, neuroticism) and team climate factors (participative safety, support for innovation, team vision and task orientation) preferences and perceptions. We aggregate the results of the three studies through a meta-analysis of correlations. The study was conducted with students. Results The aggregation of results from the baseline experiment and two replications corroborates the following findings. There is a positive relationship between all four climate factors and satisfaction in software development teams. Teams whose members score highest for the agreeableness personality factor have the highest satisfaction levels. The results unveil a significant positive correlation between the extraversion personality factor and software product quality. High participative safety and task orientation climate perceptions are significantly related to quality. Conclusions First, more efficient software development teams can be formed heeding personality factors like agreeableness and extraversion. Second, the team climate generated in software development teams should be monitored for team member satisfaction. Finally, aspects like people feeling safe giving their opinions or encouraging team members to work hard at their job can have an impact on software quality. Software project managers can take advantage of these factors to promote developer satisfaction and improve the resulting product.This research has been funded by the following projects: Experiment Replication and Synthesis Technologies in SE (MICINN TIN2011-23216) and Go Lite (MICINN TIN2011-24139)

Twenty years monitoring growth dynamics of a logged tropical forest in Western Amazon.

Resumo - Foi conduzido um estudo sobre a dinâmica de crescimento em floresta localizada no Estado do Acre na Amazônia sul ocidental, onde uma área de 20 ha foi explorada para extração de madeira em 1992. O estudo foi baseado em parcelas permanentes (1 ha) estabelecidas dentro da área explorada e na floresta não manejada, imediatamente após o fim das operações florestais. Os parâmetros da dinâmica florestal foram analisados em termos da biomassa seca acima do solo (BAS). Durante o período do estudo três eventos climáticos extremos causaram um elevado impacto tanto nas áreas exploradas como nas não perturbadas por exploração, produzindo perdas de biomassa superiores às estimadas para a exploração. Vinte anos após a exploração, as taxas de ingresso e mortalidade foram semelhantes às esperadas em uma área de floresta não perturbada por exploração e a recuperação da BAS foi significativamente mais alta nas áreas cortadas. Quando praticado de forma adequada, o manejo de floresta pode promover uma recuperação da BAS mais rápida. Nós sugerimos que o manejo florestal pode ser considerado como uma alternativa para adaptação a eventos extremos de clima por meio da promoção de perturbações controladas que minimizem a mortalidade de árvores e a perda de biomassa

Company family, innovation and colombian graphic industry: a bayesian estimation of a logistical model

This study presents a comparative analysis of the management of innovation among family and non-family companies of the Graphic Communication Industry in Colombia. For which a questionnaire was applied in order to know the divergences in the innovation process carried out by these two types of organizations. From this, the methodology of Generalized Linear Models (MLG) was used and the Bayesian inference was used on the parameters of the model, analyzing the effect of the family business, the products that commercialize on the management of innovation in goods observed as a product tangible Obtaining in this way, the identification of some characteristics of innovation management and divergences with non-family companies, among them: a tendency towards the type of preferred innovation, the different sources and objectives to innovate, and the factors that hinder its process of innovation

Prevalencia de PRM y RNM en tratamientos anticonceptivos hormonales. Eficacia de la IF en usuarias de estos tratamientos.

Objetivos- Principal: evaluación cuantitativa de los PRM y RNM asociados a los tratamientos anticonceptivos hormonales. Secundarios: Evaluar la asociación de RNM con Edad y nivel de estudios. Estudiar el grado de la intervención farmacéutica para resolver los RNM. Métodos: Estudio cuasi-experimental con 512 pacientes. Recogida de datos desde Octubre de 2015 hasta Marzo de 2016 en oficina de Farmacia Comunitaria. Para registrar y evaluar los PRM y RNM se utiliza el método DÁDER de primera visita. Cada paciente acude a tres visitas a lo largo del estudio. Resultados: En la Visita 1 se detectaron 503 RNM, en la Visita 2 se redujeron a 116 y en la Visita 3 a 45. En la Visita 1 se detectaron 853 PRM, en la Visita 2 141 y en la Visita 3 se redujeron a 90. Las intervenciones farmacéuticas son aceptadas en el 90,4% de los casos y resuelven el 91% de los RNM iniciales. Conclusiones: Casi el 82% de las pacientes usuarias de AH presentaban algún RNM. Al iniciar el estudio se detectó la presencia de más de un PRM por paciente, el tipo de PRM más prevalente es el incumplimiento, constituyendo el 52,4 % de los PRM totales. El incumplimiento puede conllevar una inefectividad del tratamiento que podría tener consecuencias tanto clínicas, como sociales y económicas. El buen resultado en cuanto a eficacia de la intervención farmacéutica invita a pensar en la necesidad de realizar este tipo de estudios con el fin de optimizar los resultados terapéuticos de los medicamentos