Responses to hydric stress in the seed-borne necrotrophic fungus Alternaria brassicicola

Alternaria brassicicola is a necrotrophic fungus causing black spot disease and is an economically important seed-borne pathogen of cultivated brassicas. Seed transmission is a crucial component of its parasitic cycle as it promotes long-term survival and dispersal. Recent studies, conducted with the Arabidopsis thaliana/A. brassicicola pathosystem, showed that the level of susceptibility of the fungus to water stress strongly influenced its seed transmission ability. In this study, we gained further insights into the mechanisms involved in the seed infection process by analyzing the transcriptomic and metabolomic responses of germinated spores of A. brassicicola exposed to water stress. Then, the repertoire of putative hydrophilins, a group of proteins that are assumed to be involved in cellular dehydration tolerance, was established in A. brassicicola based on the expression data and additional structural and biochemical criteria. Phenotyping of single deletion mutants deficient for fungal hydrophilin-like proteins showed that they were affected in their transmission to A. thaliana seeds, although their aggressiveness on host vegetative tissues remained intact

Transition alimentaire et essor économique : portrait en régions de la consommation de viandes au Vietnam

National audienc

Transition alimentaire et essor économique : portrait en régions de la consommation de viandes au Vietnam

National audienc

Effets et mécanismes d’action d'analogues structuraux de la quercétine sur la sécrétion d'insuline induite par le glucose

International audienc

Effets et mécanismes d’action d'analogues structuraux de la quercétine sur la sécrétion d'insuline induite par le glucose

International audienc

Effets et mécanismes d’action d'analogues structuraux de la quercétine sur la sécrétion d'insuline induite par le glucose

International audienc

Functional and pharmacological explorations of quercetin-like compounds on glucose-induced insulin secretion

International audienc

Functional and pharmacological explorations of quercetin-like compounds on glucose-induced insulin secretion

International audienc

Regulation of the positive transcriptional effect of PLZF through a non-canonical EZH2 activity

International audienceThe transcription factor PLZF (promyelocytic leukemia zinc finger protein) acts as an epigenetic regulator balancing self-renewal and differentiation of hematopoietic cells through binding to various chromatin-modifying factors. First described as a transcriptional repressor, PLZF is also associated with active transcription, although the molecular bases underlying the differences are unknown. Here, we reveal that in a hematopoietic cell line, PLZF is predominantly associated with transcribed genes. Additionally, we identify a new association between PLZF and the histone methyltransferase, EZH2 at the genomic level. We find that co-occupancy of PLZF and EZH2 on chromatin at PLZF target genes is not associated with SUZ12 or trimethylated lysine 27 of histone H3 (H3K27me3) but with the active histone mark H3K4me3 and active transcription. Removal of EZH2 leads to an increase of PLZF binding and increased gene expression. Our results suggest a new role of EZH2 in restricting PLZF positive transcriptional activity independently of its canonical PRC2 activity