Occurrence and Toxicological Risk Assessment of Polycyclic Aromatic Hydrocarbons and Heavy Metals in Drinking Water Resources of Southern China

Polycyclic aromatic hydrocarbons (PAHs) and heavy metals exposure is related to a variety of diseases and cancer development, posing a great health risk to humans. In this study, water samples were collected from nine important water sources in Guangdong, Guangxi and Hainan provinces to determine the degree of PAHs and heavy metals contamination. Overall, the total contents of 16 PAHs and heavy metals were found within the permissible levels. In human health risk assessment, the benzo(a)pyrene equivalent concentration (BaPeq) presented a much lower level than the guideline values announced by Chinese Environmental Protection Agency (CEPA) and United States Environmental Protection Agency (US EPA), demonstrating that the PAHs contamination level in drinking water was mostly acceptable. For heavy metals, the Chronic daily intake (CDI), hazard quotient (HQ) or hazard index (HI) suggested that the water quality in nine water sources was desirable and did not present a risk to human health

The Risk of Polychlorinated Biphenyls Facilitating Tumors in Hawaiian Green Sea Turtles (Chelonia mydas)

The Hawaiian green turtle (Chelonia mydas) is on the list of threatened species protected under the U.S. Endangered Species Act in 1978 in large part due to a severe tumor-forming disease named fibropapillomatosis. Chemical pollution is a prime suspect threatening the survival of C. mydas. In this study, PCBs concentrations were determined in 43 C. mydas plasma samples archived on Tern Island. The total PCBs concentration in male C. mydas (mean 1.10 ng/mL) was two times more than that of females (mean 0.43 ng/mL). The relationship between straight carapace length and PCBs concentration in females has also been studied, which was negatively related. To figure out the possible existence of correlations between PCBs and tumor status, we measured the PCBs concentration in turtles with no tumor, moderate or severe tumor affliction. PCBs concentration of two afflicted groups was much higher than the healthy group, suggesting that PCBs may play a role in fibropapillomatosis in Hawaiian green turtle

Dramatic red fluorescence enhancement and emission red shift of carbon dots following Zn/ZnO decoration

Dramatic red fluorescence enhancement and emission red shift of carbon dots following Zn/ZnO decoratio

Concentrations, Distribution, and Ecological Risk Assessment of Heavy Metals in Daya Bay, China

Surface sediment samples were collected from 19 sites throughout Daya Bay, China to study the concentrations, spatial distributions, potential ecological risk, and possible sources of heavy, including metals copper (Cu), zinc (Zn), arsenic (As), cadmium (Cd), nickel (Ni), lead (Pb), mercury (Hg), and chromium (Cr). The mean concentrations of the eight heavy metals were 24, 109, 6.5, 0.09, 35.3, 26.8, 0.07, and 109 µg g−1, respectively. The concentrations of most heavy metals were within range of those recorded in previous years. The spatial distribution pattern of most heavy metals were similar, with lowest values recorded along the southeast coast and the open sea area; the highest values were recorded in the northern Daya Bay, especially the northwest. Cu, Zn, As, Cd, Pb, and Hg were classified as Class I, and Ni and Cr were classified as Class II according to the Sediment Quality Guidelines (SQGs) of China. The potential ecological risk (Eif) indices of Cu, Zn, As, Pb, Ni, and Cr specify that these metals pose low risk to the ecosystem of the Bay, whereas Cd and Hg pose a very high risk in some sites. The geoaccumulation indices (Igeo) of Cu, Zn, As, Ni, and Cr specify weak or no pollution in Daya Bay, whereas those of Pb, Cd, and Hg in some sites indicate moderate or even high pollution. Spatial distribution, carbon/nitrogen analysis, Pearson correlation, and principal components analysis indicated that Cu, Zn, As, Pb, Ni, Cr, total organic carbon (TOC), and total nitrogen (TN) originated from the same sources. Ballast water or sewage from the cargo ships that park at the harbors or anchor in the Bay were the important sources for Cu, Zn, As, Pb, Ni, Cr, TOC, and TN. Other anthropogenic sources, such as agricultural runoff and aquaculture, might also be responsible, whereas Hg and Cd originated from other point sources

Microplastics in Surface Waters and Sediments from Guangdong Coastal Areas, South China

Microplastic is an emerging global pollutant that have attracted a great deal of attention from researchers and the public. Guangdong Province has a large population and a relatively well-developed economy, but lacks a modern pollution control system. Guangdong has a long coastline with varying levels of pollution, and little research has been conducted on microplastics. Therefore, we investigated the level of microplastic pollution in coastal areas of Guangdong Province, and the abundance of microplastics in surface waters and sediments at 13 sampling sites. The abundance of microplastics in surface water and sediment samples ranged from 850 to 3500 items/L and 433.3 to 4166.3 items/kg, respectively. Fiber is the main type of microplastics in both surface water and sediment. The sampled microplastics were typically transparent. Raman results show that the surface water sample mainly includes rayon (38.2%), polyethylene terephthalate (16.4%), and ethylene/vinyl acetate copolymer (12.7%). This study used the pollution load index method to evaluate the pollution risk of microplastics along the coast of Guangdong, and provided the basis for the formulation of environmental policy

Cell Signaling Pathway in 12-O-Tetradecanoylphorbol-13-acetate-Induced LCN2 Gene Transcription in Esophageal Squamous Cell Carcinoma

LCN2 is involved in various cellular functions, including transport of small hydrophobic molecules, protection of MMP9 from proteolytic degradation, and regulating innate immunity. LCN2 is elevated in multiple human cancers, frequently being associated with tumor size, stage, and invasiveness. Our previous studies have shown that LCN2 expression could be induced by 12-O-tetradecanoylphorbol-13-acetate (TPA) in esophageal squamous cell carcinoma (ESCC) by the binding of five nucleoproteins (MISP, KLF10, KLF15, PPP1R18, and RXRβ) at a novel TPA-responsive element (TRE), at −152~−60 bp of the 5′ flanking region of the LCN2 promoter. However, much is unknown about whether these proteins can respond to TPA stimulation to regulate LCN2 transactivation and which cell signaling pathways mediate this process. In this study, expression plasmids encoding these five nucleoproteins were stably transfected into EC109 cells. Then, stable transfectant was characterized by a Dual-Luciferase Reporter Assay System. RT-PCR, real-time PCR, western blotting, specific kinase inhibitor treatment, and bioinformatics analyses were applied in this study. We found that MISP, KLF10, KLF15, PPP1R18, and RXRβ proteins could strongly respond to TPA stimulation and activate LCN2 transcriptional expression. MEK, ERK, JNK, and P38 kinases were involved in the LCN2 transactivation. Furthermore, the MEK-ERK signal pathway plays a major role in this biological process but does not involve PKCα signaling