Mammary fibroadenoma in a lamb

A fibroadenoma was diagnosed in the left udder of a 3-month-old female Chios lamb. No recurrence was observed after surgery. Grossly, the tumor had a whitish-gray lobular appearance, and the lobules were interlaced with thin septa. Microscopically, the tumor was composed of proliferating fibroepithelial tissue, including differentiated ducts lined by whorls and interlacing bundles of abundant loose fibrovascular stroma. Immunohistochemistry revealed the ductal epithelium to be positive for pancytokeratin (AE1/AE3) and loose fibrovascular stroma was positive for vimentin and basal cells covering the ductal epithelium of alpha-smooth-muscle actin. Immunostaining for the estrogen and progesterone receptors was negative. A diagnosis of mammary fibroadenoma was made based on the histological and immunohistochemical findings

Cerebellum progesterone concentration decreased in canine distemper virus infection

/0000-0002-0636-4214WOS: 000244921000006PubMed: 16919304Progesterone has neuroprotective effects including augmentation of myelination in the central and peripheral nervous system. This study was designed to determine if demyelinating lesions in the cerebellum resulting from canine distemper virus (CDV) infection are associated with progesterone levels. Progesterone was measured using radioinummoassay in samples of the cerebellum, corpus callosum, medulla oblongata, parietal, frontal, temporal, and occipital cortices as well as cerebrospinal fluid (CSF) and plasma collected from ten CDV infected and six non-infected dogs. The cerebellum progesterone level was significantly different between CDV infected (0.66 +/- 0.09 ng/g) and control dogs (1.14 +/- 0.09 ng/g) (p 0.05). The cerebellum progesterone level was also significantly different between acute (0.71 +/- 0.05 ng/g) and chronic cases (0.61 +/- 0.09 ng/g) (p < 0.05). The CDV infected cerebella were also categorized histopathologically according to the severity of demyelinating lesions as mild (n = 5), moderate (n = 2), or severe (n = 3) among which the cerebellum progesterone level was significantly different (p < 0.05). Progesterone concentration was 0.71 +/- 0.05 ng/g in mild, 0.65 +/- 0.10 ng/g in moderate, and 0.56 +/- 0.07 ng/g in severe cases. In conclusion, progesterone concentration decreases in the cerebellum in CDV infection and the severity of demyelinating lesions is the greatest in cerebella with the lowest progesterone concentrations. The results suggest that local impairment of progesterone metabolism may be associated with the initiation and progression of cerebellar lesions in CDV infection. (c) 2006 Elsevier Ltd. All rights reserved

Myelin basic protein profile of central nervous system in experimentally induced demyelination and remyelination

Objective: The aim of the present study was to assess myelin basic protein (MBP) profiles of central nervous system in experimentally induced demyelination and remyelination

Comparison of lidocaine metabolism for different anesthesia techniques in rabbits with liver disease

Objective. This study was designed to investigate the serum lidocaine concentrations (SLC) after local infiltration anesthesia (IA) and mandibular anesthesias (MA) in rabbits with carbon tetrachloride (CCl4)-induced chronic liver damage (CLD)

The effects of aging on the central nervous system steroid profiles and myelin basic protein in rats

The aim of this study was to investigate the effects of aging on the central nervous system steroid and myelin basic protein (MBP) profiles. Forty-seven male Sprague-Dawley rats (newborn, 1, 6, 12 and 24-months-old) were studied. Tissues were obtained from the cerebellum and parietal, frontal, temporal cortex of the central nervous system of the rats for steroid extraction. The estradiol, progesteron, testosterone and dehydroepiandrosterone (DHEA) levels were measured by Enzyme Linked Immunosorbent Assay (ELISA). The average levels of estradiol (pg/g), progesteron (ng/g), DHEA (ng/g) and testosterone (ng/g) in the brain tissues were respectively 24.29, 4.59, 0.27, 0.92 in the newborn-rats; 4.18 +/- 1.10, 1.54 +/- 0.30, 0.28 +/- 0.01, 0.57 +/- 0.10 in the 1 month-old-rats; 11.02 +/- 1.10, 2.96 +/- 0.30, 0.27 +/- 0.01, 0.61 +/- 0.10 in the 6 month-old-rats; 15.80 +/- 1.10, 4.80 +/- 0.30, 0.28 +/- 0.10, 0.67 +/- 0.10 in the 12 month-old-rats; 20.07 +/- 1.10, 4.12 +/- 0.30, 0.28 +/- 0.01, 0.55 +/- 0.10 in the 24 month-old-rats. The myelin basic protein levels were determined by immunohistochemical staining and an elevation was observed in conjunction with the aging process. The results of the study indicate that the alterations in MBP, DHEA, progesterone, testosterone and estrodiol concentrations in the central nervous system of the rats during aging can be considered fundamental for future animal and human studies. (C) 2012, Editrice Kurti