Evaluation of fermented whole crop wheat, urea-treated processed whole crop wheat and maize silage for dairy cows

End of Project ReportThere has been increased interest in and increased usage of forages other than grass silage for feeding dairy cows during the winter period. This has arisen because of the inconsistency in making good quality grass silage and the low intake characteristics of this feed. The main objective of this project was to evaluate the effects on dairy cow intake and performance of offering fermented whole crop wheat (WCW) silage, urea-treated processed WCW and maize silage in mixtures with grass silage compared with grass silage alone. The value of these feeds as supplements to grazed grass in the Autumn for late lactation spring calving cows and their effect on dietary nitrogen (N) utilisation for milk protein production were also investigated. The first two experiments evaluated the effects of including 67% of the forage mixture on a dry matter (DM) basis as fermented WCW, urea-treated processed WCW (also known as “Alkalage”) or maize silage in comparison to grass silage alone in the diet of autumn calving cows. The fermented WCW and urea-treated processed WCW were harvested at a stubble height of approximately 20 cm. Sixty and 95% of the grain was milled/cracked in the urea-treated processed WCW in experiments 1 and 2, respectively. Forages were supplemented with concentrates of varying crude protein (CP) concentrations so as to maintain a similar CP concentration in the total dietary dry matter across treatments. In both experiments all the feeds were well preserved. The DM (g/kg) and starch contents (g/kg DM) in the fermented WCW, urea-treated processed WCW and maize silage in experiments 1 were 406 and 282, 733 and 324, 221 and 140 and in experiment 2 were 370 and 323, 763 and 341 and 302 and 324, respectively. Results from both experiments were similar. The three forage mixtures resulted in greater DM intake and greater fat plus protein production than grass silage. The largest effect on intake was obtained with the urea-treated processed WCW which probably reflected the greater DM content of this forage mixture compared with the others. Milk protein content was generally similar across the three forage mixtures and greater than on grass silage as the sole forage. In the third experiment short-straw urea-treated processed WCW (harvested at a stubble height of 35 cm) was evaluated in comparison to the fermented WCW (harvested at a stubble height of 20 cm), maize silage and grass silage. The DM (g/kg) and starch (g/kg DM) contents in the fermented WCW, urea-treated processed WCW and maize silage were 389 and 316, 795 and 382 and 346 and 301, respectively. Fifty nine percent of the grain was cracked/processed in the urea-treated processed UP-WCW .Level of inclusion of forages in the diets was the same as in the first two experiments and total dietary CP was again maintained at a similar concentration across treatments by offering concentrates of varying CP concentrations. The higher harvesting height of the urea-treated processed WCW resulted in a greater starch concentration in this feed in comparison to the first two experiments. The results however were very similar to those obtained in the first two experiments with the forage mixtures again increasing DM intake and fat plus protein yield. Inclusion of the short-straw urea-treated processed WCW did not result in greater fat plus protein production compared with the inclusion of fermented WCW or maize silage. The greatest efficiency of conversion of dietary nitrogen (N) to milk N was achieved with the maize silage mixture and the least efficiency with the urea-treated process WCW mixture. In experiment 4 the nutritive value of fermented WCW, urea-treated processed WCW and maize silage were evaluated in comparison to a concentrate as supplements to grazed grass for spring calving cows in the autumn. A treatment with a high grass allowance of 24 kgDM (> 4 cm)/cow per day was also included while the grass allowance on the supplemented treatments was restricted to 17 kgDM (> 4 cm)/cow per day. All the supplemented treatments and the high grass allowance treatment gave greater milk yields than the unsupplemented restricted grass treatment. The concentrate supplement resulted in the greatest solids corrected milk yield and this was greater than any of the forage supplemented treatments which were not significantly different from one another. In the final experiment the output of N in milk, urine and faeces was measured when grass silage, fermented WCW, urea-treated processed WCW and maize silage were fed as the sole forages plus 6 kg of a concentrate containing 338 g crude protein/kg DM. The proportion of consumed N excreted in urine and faeces was greatest on grass silage and urea-treated processed WCW with fermented WCW and maize silage resulting in the greatest proportion of consumed N being excreted in milk. Overall, the project demonstrated that including either type of WCW or maize silage with grass silage increased fat plus protein production and protein concentration to a similar extent compared to grass silage as the sole forage. All three forages increased DM intake with the greatest increase observed with urea-treated processed WCW. Because of this greater intake resulting in similar fat plus protein production conversion of dietary DM to milk solids was less efficient on urea-treated processed WCW based diets than on fermented WCW or maize silage based diets. Grass silage and urea-treated processed WCW based diets were least N efficient with less dietary N being incorporated into milk N than on fermented WCW or maize silage based diets. Comparing the responses on the forage mixtures relative to one another and to grass silage across experiments 1 to 3 indicates that neither degree of grain processing or harvesting height (within the ranges studied here) are of substantial importance in determining the nutritive value of urea-treated processed WCW. All of the three alternative forages gave similar solids corrected milk yield responses when used as buffer feeds for spring calved cows at pasture in the autumn but these responses were less than 50% of the response to a concentrate supplement

Being on the Juvenile Dermatomyositis Rollercoaster: a qualitative study

Objectives: Juvenile Dermatomyositis is a rare, potentially life-threatening condition with no known cure. There is no published literature capturinghow children and young people feel about their condition, from their perspective. This study was therefore unique in that it asked children and young people what is it like to live with Juvenile Dermatomyositis. Methods: Data wereobtained from fifteen young people with Juvenile Dermatomyositis, between eightand nineteen years of age from one Paediatric Rheumatology department using audio-recordedinterpretive phenomenology interviews. Data were analyzed phenomenologically, using a process that derives narratives from transcripts resulting in a collective composite of participants shared experiences, called a‘phenomenon’. Results:The overarching metaphor of a rollercoaster captures the phenomenon of living withJuvenile Dermatomyositisas a young person, with the ups and downs at different time points clearly described by those interviewed. The five themes plotted on the rollercoaster, began with confusion; followed by feeling different, being sick, steroidal and scared from the medications; uncertainty; and then ended with acceptance of the disease over time. Conclusion: Young people were able to talk about their experiences about having Juvenile Dermatomyositis. Our findings will aid clinicians in their practice by gaining a deeper understanding of what daily life is like and highlighting ways to enhance psychosocial functioning. Hopefully, this study and any further resulting studies,will raise understanding of Juvenile Dermatomyositis worldwide and will encourage health care professionals to better assess psychosocial needs in the future

Mapping the current psychology provision for children and young people with juvenile dermatomyositis

Objectives: Juvenile Dermatomyositis (JDM) is a rare, chronic autoimmune condition of childhood, with known psychosocial implications. In this study, we sought to establish current psychological support for children and young people across the UK with rheumatic conditions, with a specific focus on those with JDM. Methods: Electronic surveys were distributed to the 15 centres that belong to the JDM Research Group in the UK, collecting responses from health-care professionals in the fields of medicine, nursing and psychology. Results: One hundred per cent of professionals from medicine and nursing replied from all 15 centres. Of these, 7 (47%) did not have a named psychologist as part of their rheumatology team, despite the majority [13 (87%)] having >200 paediatric rheumatology patients. Of the remaining centres, hospital psychology provision varied considerably. When rating their service, only 3 (8%) of 40 professionals scored their service as five (where one is poor and five is excellent); there were wide discrepancies in these scores. Many challenges were discussed, including limited psychology provision, lack of time and difficulties in offering support across large geographical areas. Conclusion: Many of the challenges discussed are applicable to other centres worldwide. Suggestions have been proposed that might help to improve the situation for children and young people with rheumatic conditions, including JDM. Based on these findings, we suggest that rheumatology teams maximize use of these data to advocate and work toward more comprehensive psychology provision and support in their individual centres

Identification of two distinct intron elements involved in alternative splicing of beta-tropomyosin pre-mRNA

The rat beta-tropomyosin gene encodes two isoforms, termed skeletal muscle beta-tropomyosin and fibroblast last tropomyosim 1 (TM-1), via an alternative RNA processing mechanism. The gene contains 11 exons. Exons 1-5 and exons 8 and 9 are common to all mRNAs expressed from the gene. Exons 6 and 11 are used in fibroblasts, as well as smooth muscle, whereas exons 7 and 10 are used only in skeletal muscle. In the present studies we focused on the mutually exclusive internal alternative splice choice involving exon 6 (fibroblast-type splice) and exon 7 (skeletal muscle-type splice). We have identified two distinct elements in the intron, upstream of exon 7, involved in splice site selection. The first element is comprised of a polypyrimidine tract located 89-143 nucleotides upstream of the 3' splice site, which specifies the location of the lariat branchpoints used, 144-153 nucleotides upstream of exon 7. The 3' splice site AG dinucleotide has no role in selection of these branchpoints. The second element is comprised of intron sequences located between the polypyrimidine tract and the 3' splice site of exon 7. It contains an important determinant in alternative splice site selection, because deletion of these sequences results in the use of the skeletal muscle-specific exon in nonmuscle cells. We propose that the use of lariat branchpoints located far upstream from a 3' splice site may be a general feature of some alternatively excised introns, reflecting the presence of regulatory sequences located between the lariat branch site and the 3' splice site. The data also indicate that alternative splicing of the rat beta-tropomyosin gene is regulated by a somewhat different mechanism from that described for rat alpha-tropomyosin gene and the transformer-2 gene of Drosophila melanogaster

Privacy Architectures: Reasoning About Data Minimisation and Integrity

Privacy by design will become a legal obligation in the European Community if the Data Protection Regulation eventually gets adopted. However, taking into account privacy requirements in the design of a system is a challenging task. We propose an approach based on the specification of privacy architectures and focus on a key aspect of privacy, data minimisation, and its tension with integrity requirements. We illustrate our formal framework through a smart metering case study.Comment: appears in STM - 10th International Workshop on Security and Trust Management 8743 (2014

The Psychogeriatric Assessment Scales: a multidimensional alternative to categorical diagnoses of dementia and depression in the elderly

The Psychogeriatric Assessment Scales (PAS) provide an assessment of the clinical changes seen in dementia and depression. Principal components analysis and latent trait analysis were used to develop a set of scales to summarize these clinical changes. There are three scales derived from an interview with the subject (Cognitive Impairment, Depression, Stroke) and three from an interview with an informant (Cognitive Decline, Behaviour Change, Stroke). Results are reported on the reliability and validity of these scales using data from clinical samples in Sydney and Geneva and a population sample from Canberra. The scales were found to have excellent validity when judged against clinical diagnoses of dementia and depression and could distinguish Alzheimer's from vascular dementia. Cut-off points were developed to indicate correspondence between scale scores and clinical diagnoses. Percentile rank norms were developed from the Canberra population sample. The PAS is easy to administer and score and can be used by lay interviewers after training. It is intended for application both in research and in services for the elderl

Mesenchymal stem cell-based therapy for ischemic stroke

Ischemic stroke represents a major, worldwide health burden with increasing incidence. Patients affected by ischemic strokes currently have few clinically approved treatment options available. Most currently approved treatments for ischemic stroke have narrow therapeutic windows, severely limiting the number of patients able to be treated. Mesenchymal stem cells represent a promising novel treatment for ischemic stroke. Numerous studies have demonstrated that mesenchymal stem cells functionally improve outcomes in rodent models of ischemic stroke. Recent studies have also shown that exosomes secreted by mesenchymal stem cells mediate much of this effect. In the present review, we summarize the current literature on the use of mesenchymal stem cells to treat ischemic stroke. Further studies investigating the mechanisms underlying mesenchymal stem cells tissue healing effects are warranted and would be of benefit to the field

Vaginal metastasis of a Ewing sarcoma five years after resection of the primary tumor

A 35-year-old female presented with pain and swelling of the distal left radius. A diagnosis of Ewing sarcoma was made and she underwent neoadjuvant chemotherapy and surgery. Macroscopic viable areas remained on the map of the surgical specimen; as such, she was classified as a poor responder and received high dose adjuvant chemotherapy. She remained disease-free for five years, until age 40. A vaginal polyp was then detected during a routine gynaecologic examination. It was removed and histopathology revealed metastatic Ewing sarcoma

Understanding Racial HIV/STI Disparities in Black and White Men Who Have Sex with Men: A Multilevel Approach

Background: The reasons for black/white disparities in HIV epidemics among men who have sex with men have puzzled researchers for decades. Understanding reasons for these disparities requires looking beyond individual-level behavioral risk to a more comprehensive framework. Methods and Findings: From July 2010-Decemeber 2012, 803 men (454 black, 349 white) were recruited through venuebased and online sampling; consenting men were provided HIV and STI testing, completed a behavioral survey and a sex partner inventory, and provided place of residence for geocoding. HIV prevalence was higher among black (43%) versus white (13% MSM (prevalence ratio (PR) 3.3, 95% confidence interval (CI): 2.5–4.4). Among HIV-positive men, the median CD4 count was significantly lower for black (490 cells/mL) than white (577 cells/mL) MSM; there was no difference in the HIV RNA viral load by race. Black men were younger, more likely to be bisexual and unemployed, had less educational attainment, and reported fewer male sex partners, fewer unprotected anal sex partners, and less non-injection drug use. Black MSM were significantly more likely than white MSM to have rectal chlamydia and gonorrhea, were more likely to have racially concordant partnerships, more likely to have casual (one-time) partners, and less likely to discuss serostatus with partners. The census tracts where black MSM lived had higher rates of poverty and unemployment, and lower median income. They also had lower proportions of male-male households, lower male to female sex ratios, and lower HIV diagnosis rates. Conclusions: Among black and white MSM in Atlanta, disparities in HIV and STI prevalence by race are comparable to those observed nationally. We identified differences between black and white MSM at the individual, dyadic/sexual network, and community levels. The reasons for black/white disparities in HIV prevalence in Atlanta are complex, and will likely require a multilevel framework to understand comprehensively

Corridors of barchan dunes: stability and size selection

Barchans are crescentic dunes propagating on a solid ground. They form dune fields in the shape of elongated corridors in which the size and spacing between dunes are rather well selected. We show that even very realistic models for solitary dunes do not reproduce these corridors. Instead, two instabilities take place. First, barchans receive a sand flux at their back proportional to their width while the sand escapes only from their horns. Large dunes proportionally capture more than they loose sand, while the situation is reversed for small ones: therefore, solitary dunes cannot remain in a steady state. Second, the propagation speed of dunes decreases with the size of the dune: this leads -- through the collision process -- to a coarsening of barchan fields. We show that these phenomena are not specific to the model, but result from general and robust mechanisms. The length scales needed for these instabilities to develop are derived and discussed. They turn out to be much smaller than the dune field length. As a conclusion, there should exist further - yet unknown - mechanisms regulating and selecting the size of dunes.Comment: 13 pages, 13 figures. New version resubmitted to Phys. Rev. E. Pictures of better quality available on reques