Ventilation inhomogeneity in patients with cystic fibrosis measured by electrical impedance tomography

Objective: The distribution of ventilation within the lung is inhomogeneous. We hypothesized that the degree of inhomogeneity in patients with cystic fibrosis (CF) differs from that in healthy subjects. Method: Three adult patients with cystic fibrosis (CF) and two healthy subjects were recruited for this preliminary study. Subjects were asked to breathe in as deep and exhale as hard as possible three times. Electrical impedance tomography (EIT) measurements were performed during spirometry tests. The global inhomogeneity index(GI) was applied to assess the degree of ventilation homogeneity at 25%, 50%, 75% and 100% of maximum inspiratory volume. Results: Airway obstruction was detected in CF patients but not in healthy volunteers during spirometry tests. GI decreased as inspiratory volume increased in CF patients (0.59+/-0.20 at 25% and 0.39+/-0.09 at 100%, p<0.01, mean+/-SD) while GI remained unchanged in healthy subjects (0.40+/-0.05 at 25% and 0.37+/-0.03 at 100%, p=0.31). Conclusion: Degree of ventilation homogeneity appears to vary during forced respiration in CF patients, but not in healthy volunteers. EIT is a practical method to measure the inhomogeneity of ventilation distribution

Spitzer Observations of Interstellar Object 1I/`Oumuamua

1I/`Oumuamua is the first confirmed interstellar body in our Solar System. Here we report on observations of `Oumuamua made with the Spitzer Space Telescope on 2017 November 21--22 (UT). We integrated for 30.2~hours at 4.5 micron (IRAC channel 2). We did not detect the object and place an upper limit on the flux of 0.3 uJy (3sigma). This implies an effective spherical diameter less than [98, 140, 440] meters and albedo greater than [0.2, 0.1, 0.01] under the assumption of low, middle, or high thermal beaming parameter eta, respectively. With an aspect ratio for `Oumuamua of 6:1, these results correspond to dimensions of [240:40, 341:57, 1080:180] meters, respectively. We place upper limits on the amount of dust, CO, and CO2 coming from this object that are lower than previous results; we are unable to constrain the production of other gas species. Both our size and outgassing limits are important because `Oumuamua's trajectory shows non-gravitational accelerations that are sensitive to size and mass and presumably caused by gas emission. We suggest that `Oumuamua may have experienced low-level post-perihelion volatile emission that produced a fresh, bright, icy mantle. This model is consistent with the expected eta value and implied high albedo value for this solution, but, given our strict limits on CO and CO2, requires another gas species --- probably H2O --- to explain the observed non-gravitational acceleration. Our results extend the mystery of `Oumuamua's origin and evolution

Implications of the Small Spin Changes Measured for Large Jupiter-Family Comet Nuclei

Rotational spin-up due to outgassing of comet nuclei has been identified as a possible mechanism for considerable mass-loss and splitting. We report a search for spin changes for three large Jupiter-family comets (JFCs): 14P/Wolf, 143P/Kowal-Mrkos, and 162P/Siding Spring. None of the three comets has detectable period changes, and we set conservative upper limits of 4.2 (14P), 6.6 (143P) and 25 (162P) minutes per orbit. Comparing these results with all eight other JFCs with measured rotational changes, we deduce that none of the observed large JFCs experiences significant spin changes. This suggests that large comet nuclei are less likely to undergo rotationally-driven splitting, and therefore more likely to survive more perihelion passages than smaller nuclei. We find supporting evidence for this hypothesis in the cumulative size distributions of JFCs and dormant comets, as well as in recent numerical studies of cometary orbital dynamics. We added 143P to the sample of 13 other JFCs with known albedos and phase-function slopes. This sample shows a possible correlation of increasing phase-function slopes for larger geometric albedos. Partly based on findings from recent space missions to JFCs, we hypothesise that this correlation corresponds to an evolutionary trend for JFCs. We propose that newly activated JFCs have larger albedos and steeper phase functions, which gradually decrease due to sublimation-driven erosion. If confirmed, this could be used to analyse surface erosion from ground and to distinguish between dormant comets and asteroids

Earth-based detection of the millimetric thermal emission of the nucleus of comet 8P/Tuttle

Little is known about the physical properties of cometary nuclei. Apart from space mission targets, measuring the thermal emission of a nucleus is one of the few means to derive its size, independently of its albedo, and to constrain some of its thermal properties. This emission is difficult to detect from Earth but space telescopes (Infrared Space Observatory, Spitzer Space Telescope, Herschel Space Observatory) allow reliable measurements in the infrared and the sub-millimetre domains. We aim at better characterizing the thermal properties of the nucleus of comet 8P/Tuttle using multi-wavelentgh space- and ground-based observations, in the visible, infrared, and millimetre range. We used the Plateau de Bure Interferometer to measure the millimetre thermal emission of comet 8P/Tuttle at 240 GHz (1.25 mm) and analysed the observations with the shape model derived from Hubble Space Telescope observations and the nucleus size derived from Spitzer Space Telescope observations. We report on the first detection of the millimetre thermal emission of a cometary nucleus since comet C/1995 O1 Hale-Bopp in 1997. Using the two contact spheres shape model derived from Hubble Space Telescope observations, we constrained the thermal properties of the nucleus. Our millimetre observations are best match with: i) a thermal inertia lower than ~10 J K-1 m-2 s-1/2, ii) an emissivity lower than 0.8, indicating a non-negligible contribution of the colder sub-surface layers to the outcoming millimetre flux.Comment: 7 pages. Accepted for publication in Astronomy & Astrophysic

Emerging Capabilities for Detection and Characterization of Near-Earth Objects (NEOs)

Here we describe the status for the detection and characterization of Near- Earth Objects (NEO) with current and future observatories. A summary of the capabilities, limitations, and obtainable NEO parameters is provided. <p/

Molecular preservation by extraction and fixation, mPREF: a method for small molecule biomarker analysis and histology on exactly the same tissue

<p>Abstract</p> <p>Background</p> <p>Histopathology is the standard method for cancer diagnosis and grading to assess aggressiveness in clinical biopsies. Molecular biomarkers have also been described that are associated with cancer aggressiveness, however, the portion of tissue analyzed is often processed in a manner that is destructive to the tissue. We present here a new method for performing analysis of small molecule biomarkers and histology in exactly the same biopsy tissue.</p> <p>Methods</p> <p>Prostate needle biopsies were taken from surgical prostatectomy specimens and first fixed, each in a separate vial, in 2.5 ml of 80% methanol:water. The biopsies were fixed for 24 hrs at room temperature and then removed and post-processed using a non-formalin-based fixative (UMFIX), embedded, and analyzed by hematoxylin and eosin (H&E) and by immunohistochemical (IHC) staining. The retained alcohol pre-fixative was analyzed for small molecule biomarkers by mass spectrometry.</p> <p>Results</p> <p>H&E analysis was successful following the pre-fixation in 80% methanol. The presence or absence of tumor could be readily determined for all 96 biopsies analyzed. A subset of biopsy sections was analyzed by IHC, and cancerous and non-cancerous regions could be readily visualized by PIN4 staining. To demonstrate the suitability for analysis of small molecule biomarkers, 28 of the alcohol extracts were analyzed using a mass spectrometry-based metabolomics platform. All extracts tested yielded successful metabolite profiles. 260 named biochemical compounds were detected in the alcohol extracts. A comparison of the relative levels of compounds in cancer containing <it>vs</it>. non-cancer containing biopsies showed differences for 83 of the compounds. A comparison of the results with prior published reports showed good agreement between the current method and prior reported biomarker discovery methods that involve tissue destructive methods.</p> <p>Conclusions</p> <p>The Molecular Preservation by Extraction and Fixation (mPREF) method allows for the analysis of small molecule biomarkers from exactly the same tissue that is processed for histopathology.</p

Metabolomics: A Novel Approach to Early and Noninvasive Prostate Cancer Detection

Prostate cancer (PCa) is the most commonly diagnosed visceral cancer in men and is responsible for the second highest cancer-related male mortality rate in Western countries, with increasing rates being reported in Korea, Japan, and China. Considering the low sensitivity of prostate-specific antigen (PSA) testing, it is widely agreed that reliable, age-independent markers of the presence, nature, and progression of PCa are required to facilitate diagnosis and timely treatment. Metabolomics or metabonomics has recently emerged as a novel method of PCa detection owing to its ability to monitor changes in the metabolic signature, within biofluids or tissue, that reflect changes in phenotype and function. This review outlines the physiology of prostate tissue and prostatic fluid in health and in malignancy in relation to metabolomics as well as the principles underlying the methods of metabolomic quantification. Promising metabolites, metabolic profiles, and their correlation with the presence and stage of PCa are summarized. Application of metabolomics to biofluids and in vivo quantification as well as the direction of current research in supplementing and improving current methods of detection are discussed. The current debate in the urology literature on sarcosine as a potential biomarker for PCa is reviewed and discussed. Metabolomics promises to be a valuable tool in the early detection of PCa that may enable earlier treatment and improved clinical outcomes