Agriculture in the Fredericksburg area

This paper is a study of agriculture and rural conditions in the Virginia counties of Caroline, Culpeper, Orange, Spotsylvania, and Stafford from 1800 to 1840. These counties are located in the north-central section of the state. The easternmost of the counties, Caroline, is located on the edge of the Tidewater section; Orange and Culpeper, in the Piedmont, extend to the foothills of the Blue Ridge Mountains. The land is generally flat in the east, becomes more rolling as it approaches the mountains. Soils range from sandy loams in Caroline County to the sandy and clay loams with underlying crystalline rock formations in the Piedmont. The soils vary in productivity depending upon the topography, rainfall, texture, and underlying rock formation. Although not as rich as the limestone soils of the valley region, generally the soils of the Fredericksburg area are of moderate fertility

Capitalising on faith? An intergenerational study of social and religious capital among Baby Boomers and Millennials in Britain

This is the final version. Available on open access from SAGE Publications via the DOI in this recordIntergenerational inequalities in economic security, health, and political participation are frequently associated with inequalities in access to social capital. Millennials (those born…) are often regarded as the least civically active generation, suggesting that they have less access to social capital, compared to other generations. Numerous studies have linked the decline of religion with falling social capital, as younger generations are deprived of a valuable source of social interaction; others, however, have claimed the link between the two is spurious because Millennials have developed different ways of interacting with social institutions and each other. Despite various studies exploring links between forms of religious and social capital, the role of religious decline in contributing to the intergenerational inequalities of today remains unclear. This study examines how religious capital is related to social capital for Baby Boomers and Millennials in the UK. Our analysis shows that while lower levels of religious capital are contributing to lower levels of social capital among Millennials, religious activity is also a more effective source of social capital for Millennials than their elders. We discuss possible interpretations of our data, including exploring whether greater religious engagement among Millennials may protect against intergenerational inequality and conflict.Economic and Social Research Council (ESRC

Hospital sink traps as a potential source of the emerging multidrug-resistant pathogen Cupriavidus pauculus: characterization and draft genome sequence of strain MF1

This is the final version. Available on open access from the Microbiology Society via the DOI in this recordData availability: This project has been deposited at DDBJ/ENA/GenBank under the accession JAIQWY000000000 within BioProject PRJNA762702. The version described in this paper is version JAIQWY010000000. The contig sequences can be found under accession numbers JAIQWY010000001–JAIQWY010000005. Raw sequence data have been deposited under accession numbers SRX13365403 and SRX13365404. Four supplementary tables are available with the online version of this article.Introduction. Cupriavidus pauculus is historically found in soil and water but has more recently been reported to cause human infection and death. Hospital sink traps can serve as a niche for bacterial persistence and a platform for horizontal gene transfer, with evidence of dissemination of pathogens in hospital plumbing systems driving nosocomial infection.Gap Statement. This paper presents the first C. pauculus strain isolated from a hospital sink trap. There are only six genome assemblies available on NCBI for C. pauculus; two of these are PacBio/Illumina hybrids. This paper presents the first ONT/Illumina hybrid assembly, with five contigs. The other assemblies available consist of 37, 38, 111 and 227 contigs. This paper also presents data on biofilm formation and lethal dose in Galleria mellonella; there is little published information describing these aspects of virulence.Aim. The aims were to identify the isolate found in a hospital sink trap, characterize its genome, and assess whether it could pose a risk to human health.Methodology. The genome was sequenced, and a hybrid assembly of short and long reads produced. Antimicrobial susceptibility was determined by the broth microdilution method. Virulence was assessed by measuring in vitro biofilm formation compared to Pseudomonas aeruginosa and in vivo lethality in Galleria mellonella larvae.Results. The isolate was confirmed to be a strain of C. pauculus, with a 6.8 Mb genome consisting of 6468 coding sequences and an overall G+C content of 63.9 mol%. The genome was found to contain 12 antibiotic resistance genes, 8 virulence factor genes and 33 metal resistance genes. The isolate can be categorized as resistant to meropenem, amoxicillin, amikacin, gentamicin and colistin, but susceptible to cefotaxime, cefepime, imipenem and ciprofloxacin. Clear biofilm formation was seen in all conditions over 72 h and exceeded that of P. aeruginosa when measured at 37 °C in R2A broth. Lethality in G. mellonella larvae over 48 h was relatively low.Conclusion. The appearance of a multidrug-resistant strain of C. pauculus in a known pathogen reservoir within a clinical setting should be considered concerning. Further work should be completed to compare biofilm formation and in vivo virulence between clinical and environmental strains, to determine how easily environmental strains may establish human infection. Infection control teams and clinicians should be aware of the emerging nature of this pathogen and further work is needed to minimize the impact of contaminated hospital plumbing systems on patient outcomes

Software Lock Mass by Two-Dimensional Minimization of Peptide Mass Errors

Mass accuracy is a key parameter in proteomic experiments, improving specificity, and success rates of peptide identification. Advances in instrumentation now make it possible to routinely obtain high resolution data in proteomic experiments. To compensate for drifts in instrument calibration, a compound of known mass is often employed. This ‘lock mass’ provides an internal mass standard in every spectrum. Here we take advantage of the complexity of typical peptide mixtures in proteomics to eliminate the requirement for a physical lock mass. We find that mass scale drift is primarily a function of the m/z and the elution time dimensions. Using a subset of high confidence peptide identifications from a first pass database search, which effectively substitute for the lock mass, we set up a global mathematical minimization problem. We perform a simultaneous fit in two dimensions using a function whose parameterization is automatically adjusted to the complexity of the analyzed peptide mixture. Mass deviation of the high confidence peptides from their calculated values is then minimized globally as a function of both m/z value and elution time. The resulting recalibration function performs equal or better than adding a lock mass from laboratory air to LTQ-Orbitrap spectra. This ‘software lock mass’ drastically improves mass accuracy compared with mass measurement without lock mass (up to 10-fold), with none of the experimental cost of a physical lock mass, and it integrated into the freely available MaxQuant analysis pipeline (www.maxquant.org)

'So wide and varied': The origins and character of British information science

This paper examines some characteristics of the ‘British School’ of information science. Three main forces driving the development of the new subject in Britain are identified: the documentation movement; special libraries; and the need for better treatment of scientific and technical information. Five characteristics which, taken together, distinguish the early British approach to information science from those adopted elsewhere are identified: its subject-based nature; its broad approach to information and information science; its status as an academic subject with a strong professional remit; its involvement with, but distinction from, information technology; and its involvement with memory institutions. Lessons are drawn for the future development of the information sciences

Plasmid fitness costs are caused by specific genetic conflicts enabling resolution by compensatory mutation

Plasmids play an important role in bacterial genome evolution by transferring genes between lineages. Fitness costs associated with plasmid carriage are expected to be a barrier to gene exchange, but the causes of plasmid fitness costs are poorly understood. Single compensatory mutations are often sufficient to completely ameliorate plasmid fitness costs, suggesting that such costs are caused by specific genetic conflicts rather than generic properties of plasmids, such as their size, metabolic burden, or gene expression level. By combining the results of experimental evolution with genetics and transcriptomics, we show here that fitness costs of 2 divergent large plasmids in Pseudomonas fluorescens are caused by inducing maladaptive expression of a chromosomal tailocin toxin operon. Mutations in single genes unrelated to the toxin operon, and located on either the chromosome or the plasmid, ameliorated the disruption associated with plasmid carriage. We identify one of these compensatory loci, the chromosomal gene PFLU4242, as the key mediator of the fitness costs of both plasmids, with the other compensatory loci either reducing expression of this gene or mitigating its deleterious effects by up-regulating a putative plasmid-borne ParAB operon. The chromosomal mobile genetic element Tn6291, which uses plasmids for transmission, remained up-regulated even in compensated strains, suggesting that mobile genetic elements communicate through pathways independent of general physiological disruption. Plasmid fitness costs caused by specific genetic conflicts are unlikely to act as a long-term barrier to horizontal gene transfer (HGT) due to their propensity for amelioration by single compensatory mutations, helping to explain why plasmids are so common in bacterial genomes.</jats:p