Evaluación de las asignaciones presupuestarias de proyectos de inversión pública del área urbana del cantón Riobamba y su incidencia en el desarrollo local, período 2015-2016/Evaluation of budget allocations for public investment projects in the urban are

Resumen El principal objetivo del presente ensayo es evaluar las asignaciones presupuestarias de los proyectos de inversión pública en el área urbana del Cantón Riobamba y su incidencia en el Desarrollo Local en el período 2015-2016, el presente trabajo de investigación fue correlacionar porque permitió conocer la relación o grado de asociación que existió entre la inversión pública, las asignaciones presupuestarias y el desarrollo local; a través de la aplicación de las variables de análisis estadístico: ANOVA y LEVENE; determinando así, cómo influyó esta relación de variables en mejorar la calidad de vida de la población considerando una correcta visión de desarrollo local en sus dimensiones: económica, social y territorial. De tal forma que se realice una revisión a la gestión realizada en la elaboración, ejecución y evaluación presupuestaria de los recursos económicos provistos por el Gobierno Local y Nacional; su uso, su beneficio y los resultados obtenidos, que permitan un aporte significativo en la evaluación de la inclusión de un adecuado modelo de ordenamiento territorial previsto a través de la Constitución y leyes vigentes para verificar que la ciudadanía se encuentre en un entorno ordenado, organizado y con acceso a un desarrollo local sostenible y sustentable en el tiempo. The main objective of this essay is to evaluate the budget allocations of public investment projects in the urban area of the Riobamba Canton and its impact on Local Development in the period 2015-2016, the present research work was correlated because it allowed to know the relationship or degree of association that existed between public investment, budget allocations and local development; through the application of statistical analysis variables: ANOVA and LEVENE; determining how this relationship of variables influenced to improve the quality of life of the population considering a correct vision of local development in its dimensions: economic, social and territorial. In such a way that a review is made of the management carried out in the preparation, execution and budgetary evaluation of the economic resources provided by the Local and National Government; its use, its benefit and the results obtained, which allow a significant contribution in the evaluation of the inclusion of an adequate model of territorial ordering foreseen through the Constitution and laws in force to verify that citizenship is in an orderly, organized environment and with access to sustainable local development that is sustainable over time. Palabras clave: Ordenamiento Territorial, Territorio, Presupuesto, Desarrollo Local, Evaluación. Keywords: Territorial Planning, Territory, Budget, Local Development, Evaluation

Understanding and controlling the structure and segregation behaviour of AuRh nanocatalysts

Heterogeneous catalysis, which is widely used in the chemical industry, makes a great use of supported late-transition-metal nanoparticles, and bimetallic catalysts often show superior catalytic performances as compared to their single metal counterparts. In order to optimize catalyst efficiency and discover new active combinations, an atomic-level understanding and control of the catalyst structure is desirable. In this work, the structure of catalytically active AuRh bimetallic nanoparticles prepared by colloidal methods and immobilized on rutile titania nanorods was investigated using aberration-corrected scanning transmission electron microscopy. Depending on the applied post-treatment, different types of segregation behaviours were evidenced, ranging from Rh core-Au shell to Janus via Rh ball-Au cup configuration. The stability of these structures was predicted by performing density-functional-theory calculations on unsupported and titania-supported Au-Rh clusters; it can be rationalized from the lower surface and cohesion energies of Au with respect to Rh, and the preferential binding of Rh with the titania support. The bulk-immiscible AuRh/TiO2 system can serve as a model to understand similar supported nanoalloy systems and their synergistic behaviour in catalysis

A computational map of the human-SARS-CoV-2 protein-RNA interactome predicted at single-nucleotide resolution

RNA-binding proteins (RBPs) are critical host factors for viral infection, however, large scale experimental investigation of the binding landscape of human RBPs to viral RNAs is costly and further complicated due to sequence variation between viral strains. To fill this gap, we investigated the role of RBPs in the context of SARS-CoV-2 by constructing the first in silico map of human RBP-viral RNA interactions at nucleotide-resolution using two deep learning methods (pysster and DeepRiPe) trained on data from CLIP-seq experiments on more than 100 human RBPs. We evaluated conservation of RBP binding between six other human pathogenic coronaviruses and identified sites of conserved and differential binding in the UTRs of SARS-CoV-1, SARS-CoV-2 and MERS. We scored the impact of mutations from 11 variants of concern on protein-RNA interaction, identifying a set of gain- and loss-of-binding events, as well as predicted the regulatory impact of putative future mutations. Lastly, we linked RBPs to functional, OMICs and COVID-19 patient data from other studies, and identified MBNL1, FTO and FXR2 RBPs as potential clinical biomarkers. Our results contribute towards a deeper understanding of how viruses hijack host cellular pathways and open new avenues for therapeutic intervention

Diameter-dependent release of a cisplatin pro-drug from small and large functionalized carbon nanotubes

The use of platinum-based chemotherapeutic drugs in cancer therapy still suffers from severe disadvantages, such as lack of appropriate selectivity for tumor tissues and insurgence of multi-drug resistance. Moreover, drug efficacy can be attenuated by several mechanisms such as premature drug inactivation, reduced drug uptake inside cells and increased drug efflux once internalized. The use of functionalized carbon nanotubes (CNTs) as chemotherapeutic drug delivery systems is a promising strategy to overcome such limitations due to their ability to enhance cellular internalization of poorly permeable drugs and thus increase the drug bioavailability at the diseased site, compared to the free drug. Furthermore, the possibility to encapsulate agents in the nanotubes’ inner cavity can protect the drug from early inactivation and their external functionalizable surface is useful for selective targeting. In this study, a hydrophobic platinum(IV) complex was encapsulated within the inner space of two different diameter functionalized multi-walled CNTs (Pt(IV)@CNTs). The behavior of the complexes, compared to the free drug, was investigated on both HeLa human cancer cells and RAW 264.7 murine macrophages. Both CNT samples efficiently induced cell death in HeLa cancer cells 72 hours after the end of exposure to CNTs. Although the larger diameter CNTs were more cytotoxic on HeLa cells compared to both the free drug and the smaller diameter nanotubes, the latter allowed a prolonged release of the encapsulated drug, thus increasing its anticancer efficacy. In contrast, both Pt(IV)@CNT constructs were poorly cytotoxic on macrophages and induced negligible cell activation and no pro-inflammatory cytokine production. Both CNT samples were efficiently internalized by the two types of cells, as demonstrated by transmission electron microscopy observations and flow cytometry analysis. Finally, the platinum levels found in the cells after Pt(IV)@CNT exposure demonstrate that they can promote drug accumulation inside cells in comparison with treatment with the free complex. To conclude, our study shows that CNTs are promising nanocarriers to improve the accumulation of a chemotherapeutic drug and its slow release inside tumor cells, by tuning the CNT diameter, without inducing a high inflammatory response

Non-Invasive Exploration of Neonatal Gastric Epithelium by Using Exfoliated Epithelial Cells

Background & Aims: In preterm infants, exfoliated gastric epithelial cells can be retrieved from aspirates sampled through the naso-gastric feeding tube. Our aims were to determine (1) whether the recovery of exfoliated cells is feasible at any time from birth through the removal of the nasogastric tube, (2) whether they can be grown in culture in vitro, and (3) whether the physiological state of exfoliated cells expressing H+/K+-ATPases reflects that of their counterparts remaining in situ at the surface of the gastric epithelium in neonatal rat pups. Methods: In infants, gastric fluid aspirates were collected weekly after birth or every 3 hours over 24-h periods, and related to clinical parameters (Biocollection PROG/09/18). In rat pups submitted to a single fasting/refeeding cycle, we explored circadian exfoliation with the cellular counter-parts in the gland. All samples were analyzed by confocal imaging and Enzyme-Linked Immunosorbent Assay. Results: Epithelial cells were identified by microscopy using membrane-bound anti-H+/K+ ATPases antibody, assessed for nucleus integrity, and the expression of selected proteins (autophagy, circadian clock). On 34 infants, the H+/K+-ATPasepositive cells were consistently found quiescent, regardless of gestational age and feeding schedule from day-5 of life to the day of removal of the naso-gastric tube. By logistic regression analysis, we did find a positive correlation between the intensity of exfoliation (cellular loss per sample) and the postnatal age (p,0.001). The H+/K+ ATPase-positive cell

L'HYDROPTERE: HOW MULTIDISCIPLINARY SCIENTIFIC RESEARCH MAY HELP BREAK THE SAILING SPEED RECORD

In 2009, l’Hydroptère broke the symbolic barrier of 50 knots and became the world fastest sailing boat over both 500 meters and 1 nautical mile. This major achievement relied on the high skills of the sailing team but also on technical advances of the boat, resulting from the scientific collaboration between the Hydroptère Design Team and the Ecole Polytechnique Fédérale de Lausanne (EPFL). In the present article, we highlight the multidisciplinary research activity performed within EPFL in the course of this collaboration involving aero- and hydrodynamics, materials and structure as well as computer vision. Various foils were tested at reduced scale in a high speed water tunnel, and the results used to validate the numerical simulations. Composite materials, their processing parameters and assembly components were tested. The structural behaviour was also investigated to determine strains and stresses in normal and extreme sailing conditions, taking waves into account, and a combined model was derived for dynamic simulation. Finally, advanced computer vision methods were developed and implemented on the boat to monitor foil immersion and cross beams deformations

The regulatory mechanisms of NG2/CSPG4 expression

Neuron-glial antigen 2 (NG2), also known as chondroitin sulphate proteoglycan 4 (CSPG4), is a surface type I transmembrane core proteoglycan that is crucially involved in cell survival, migration and angiogenesis. NG2 is frequently used as a marker for the identification and characterization of certain cell types, but little is known about the mechanisms regulating its expression. In this review, we provide evidence that the regulation of NG2 expression underlies inflammation and hypoxia and is mediated by methyltransferases, transcription factors, including Sp1, paired box (Pax) 3 and Egr-1, and the microRNA miR129-2. These regulatory factors crucially determine NG2-mediated cellular processes such as glial scar formation in the central nervous system (CNS) or tumor growth and metastasis. Therefore, they are potential targets for the establishment of novel NG2-based therapeutic strategies in the treatment of CNS injuries, cancer and other conditions of these types

The zinc transporter ZIP12 regulates the pulmonary vascular response to chronic hypoxia

The typical response of the adult mammalian pulmonary circulation to a low oxygen environment is vasoconstriction and structural remodelling of pulmonary arterioles, leading to chronic elevation of pulmonary artery pressure (pulmonary hypertension) and right ventricular hypertrophy. Some mammals, however, exhibit genetic resistance to hypoxia-induced pulmonary hypertension1, 2, 3. We used a congenic breeding program and comparative genomics to exploit this variation in the rat and identified the gene Slc39a12 as a major regulator of hypoxia-induced pulmonary vascular remodelling. Slc39a12 encodes the zinc transporter ZIP12. Here we report that ZIP12 expression is increased in many cell types, including endothelial, smooth muscle and interstitial cells, in the remodelled pulmonary arterioles of rats, cows and humans susceptible to hypoxia-induced pulmonary hypertension. We show that ZIP12 expression in pulmonary vascular smooth muscle cells is hypoxia dependent and that targeted inhibition of ZIP12 inhibits the rise in intracellular labile zinc in hypoxia-exposed pulmonary vascular smooth muscle cells and their proliferation in culture. We demonstrate that genetic disruption of ZIP12 expression attenuates the development of pulmonary hypertension in rats housed in a hypoxic atmosphere. This new and unexpected insight into the fundamental role of a zinc transporter in mammalian pulmonary vascular homeostasis suggests a new drug target for the pharmacological management of pulmonary hypertension