Child selfperceptions of general and oral health in Puebla, México

Objetivo: Determinar la auto-percepción de la salud en general y bucal de los niños evaluados. Material y método: Estudio observacional, descriptivo, transversal, homodémico y prolectivo en 235 niños y niñas de 8 a 12 años de edad quienes cumplieron con los criterios de selección; que aceptaran participar en el estudio, cuyos padres firmaron el consentimiento informado. Se preguntó a cada niño sobre el estado de su salud en general y bucal. Resultado: La mayoría de los niños y niñas tienen una mejor auto-percepción de la salud en general con un 82.2% calificada como excelente, muy buena y buena y sólo el 17.9% como regular, mientras que para la salud bucal, sólo el 56.9% la calificó como excelente, muy buena y buena, el 39.6% como regular y el 3.9% como deficiente. Conclusión: La autopercepción de la salud bucal es más deficiente que la autopercepción de la salud en general en los niños mexicanos de 8 a 10 años, lo cual pudiera impactar en las conductas que presentan para mantener dichos estados de salud.Objective: To determine the self-perceived general and oral health of children evaluated. Materials and methods: Observational, descriptive, transversal, and homodemic prolective in 235 children aged 8 to 12 years of age who met the selection criteria, agree to take part in the study whose parents signed informed consent. Each child was asked about the status of your general health and oral. Results: Most children have a better self-perceived health in general, that oral health. Conclusion: We conclude that the information presented makes evident the feelings of the children about how to place their subjective well-being in general and oral health, which affects the behaviors that have to maintain those health states.Facultad de Ciencias Médica

Temporomandibular disorders among Brazilian adolescents: reliability and validity of a screening questionnaire

Temporomandibular disorders (TMD) screeners assume significant item overlap with the screening questionnaire proposed by the American Academy of Orofacial Pain (AAOP). Objective: To test the reliability and validity of the Portuguese version of AAOP questions for TMD screening among adolescents. Material and Methods: Diagnoses from Research Diagnostic Criteria for Temporomandibular Disorders (RDC/TMD) Axis I were used as reference standard. Reliability was evaluated by internal consistency (KR-20) and inter-item correlation. Validity was tested by sensitivity, specificity, predictive values, accuracy and receiver operating characteristic (ROC) curves, the relationship between the true-positive rate (sensitivity) and the false-positive rate (specificity). Test-retest reliability of AAOP questions and intra-examiner reproducibility of RDC/TMD Axis I were tested with kappa statistics. Results: The sample consisted of 1307 Brazilian adolescents (56.8% girls; n=742), with mean age of 12.72 years (12.69 F/12.75 M). According to RDC/TMD, 397 [30.4% (32.7% F/27.3% M)] of adolescents presented TMD, of which 330 [25.2% (27.6% F/22.2% M)] were painful TMD. Because of low consistency, items #8 and #10 of the AAOP questionnaire were excluded. Remaining items (of the long questionnaire version) showed good consistency and validity for three positive responses or more. After logistic regression, items #4, #6, #7 and #9 also showed satisfactory consistency and validity for two or more positive responses (short questionnaire version). Both versions demonstrated excellent specificity (about 90%), but higher sensitivity for detecting painful TMD (78.2%). Better reproducibility was obtained for the short version (k=0.840). Conclusions: The Portuguese version of AAOP questions showed both good reliability and validity for the screening of TMD among adolescents, especially painful TMD, according to RDC/TMD

Prevalencia de signos y síntomas de trastornos temporomandibulares en niños de Puebla, México, evaluados con los criterios de investigación diagnóstica para trastornos temporomandibulares (CDI/TMD)

El objetivo del presente trabajo fue describir la prevalencia de signos y síntomas de los trastornos temporomandibulares (TTM) en niños del estado de Puebla, México. Se realizó un estudio observacional descriptivo. Previo cálculo de tamaño de muestra, se incluyeron niños que cumplieron con criterios previos de selección: inscriptos en escuela primaria oficial, de cualquier sexo, en edades de 8 a 12 años, que aceptaron participar en el estudio y cuyos padres firmaron el consentimiento informado. Se utilizaron los Criterios de Investigación Diagnóstica para los TTM, aplicados por investigador estandarizado (Kappa .90) bajo las mismas condiciones. Se calculó estadística descriptiva con el programa SPSS v15. Se incluyeron 235 niños, 129 (54.9%) mujeres y 106 (45.1%) varones con promedio de edad de 9.31±1.2 años. La prevalencia de signos y síntomas de TTM fue del 33.2% predominantemente musculares (82%), 48.1% presentaron dolor muscular y 19.1% articular. El 63.4% presentó alteraciones en el patrón de apertura bucal, 39.1% presentó ruidos articulares en apertura o cierre y 20.4% a las excursiones mandibulares. Las altas prevalencias de los signos y síntomas relacionadas con los Trastornos Temporomandibulares, particularmente en niños con dentición mixta, demuestran la importancia de la evaluación de los TTM durante este periodo, donde prevalecen los cambios morfológicos asociados al crecimiento y al desarrollo craneofacial.The aim of this work was to describe the prevalence of signs and symptoms of temporomandibular disorders (TMD) in children of the State of Puebla, Mexico. A descriptive observational study was performed. After calculating sample size, children who met the following selection criteria were included: registered at an official elementary school, either sex, ages between 8 and 12 years, who accept to participate in the study and whose parents have signed the informed consent forms. The Research Diagnostic Criteria for Temporomandibular Disorders were used by calibrated researchers (Kappa.90) under the same conditions. Descriptive statistics were applied by using SPSSv15 software. The study included 235 children, 129 (54.9%) female and 106 (45.1%) male, of average age 9.31+1.2 years. Prevalence of signs and symptoms was 33.2%, and predominately muscular (82%), 48.1% showed signs of muscular pain and 19.1% joint pain. 63.4% showed signs of alteration in the mouth opening pattern, 39.1% presented joint sounds on opening or closing the mouth and 20.4% on mandibular excursions. The high prevalence of signs and symptoms related to temporomandibular disorders, in particular in children with mixed dentition, shows the importance of TMD evaluation during this period, when morphological changes associated to growth and craniofacial development prevail.Fil: Moyaho Bernal, Angeles. Benemérita Universidad Autónoma de Puebla; MéxicoFil: Lara Muñoz, María del Carmen. Benemérita Universidad Autónoma de Puebla; MéxicoFil: Espinosa De Santillana, Irene. Benemérita Universidad Autónoma de Puebla; MéxicoFil: Etchegoyen, Graciela Susana. Universidad Nacional de la Plata. Facultad de Cs.médicas. Centro Interdisc.universitario Para la Salud; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentin

Prevalence of signs and symptoms of temporomandibular disorders in children in the state of Puebla, Mexico, evaluated with the research diagnostic criteria for temporomandibular disorders (RDC/TMD)

"The aim of this work was to describe the prevalence of signs and symptoms of temporomandibular disorders (TMD) in children of the State of Puebla, Mexico. A descriptive observational study was performed. After calculating sample size, children who met the following selection criteria were included: registered at an official elementary school, either sex, ages between 8 and 12 years, who accept to participate in the study and whose parents have signed the informed consent forms. The Research Diagnostic Criteria for Temporomandibular Disorders were used by calibrated researchers (Kappa.90) under the same conditions. Descriptive statistics were applied by using SPSSv15 software. The study included 235 children, 129 (54.9%) female and 106 (45.1%) male, of average age 9.31+1.2 years. Prevalence of signs and symptoms was 33.2%, and predominately muscular (82%), 48.1% showed signs of muscular pain and 19.1% joint pain. 63.4% showed signs of alteration in the mouth opening pattern, 39.1% presented joint sounds on opening or closing the mouth and 20.4% on mandibular excursions. The high prevalence of signs and symptoms related to temporomandibular disorders, in particular in children with mixed dentition, shows the importance of TMD evaluation during this period, when morphological changes associated to growth and craniofacial development prevail"

Chemical Changes of Enamel Produced by Sodium Fluoride, Hydroxyapatite, Er:YAG Laser, and Combined Treatments

Occlusal pits and fissures of permanent molars are considered to have higher risk of developing caries. Enamel demineralization can be prevented by applying remineralizing agents, and their absorption increases with prior irradiation. This work evaluates the chemical changes produced by treating occlusal surfaces with sodium fluoride (NaF), hydroxyapatite-NaF-xylitol (HA-NaF-X), Er:YAG laser irradiation (L), and combinations thereof. Fifty enamel samples were randomly assigned to five groups (n=10): NaF, HA-NaF-X, L, L + NaF, and L + HA-NaF-X. The chemical composition of human enamel was evaluated before (BT) and after (AT) treatment using energy-dispersive X-ray spectroscopy (EDS) and expressed in atomic percentages (at%). For combined treatment groups, the products were applied after laser irradiation. The statistical analyses included a paired t-test and ANOVA (p≤0.05). After treatment, a significant increase in F at% was observed in the NaF group (2.71 ± 1.41). The irradiated groups showed significant increases in Ca and P at% and the Ca/P ratio. The highest values occurred for L + NaF (30.44 ± 4.28 Ca at%, 11.97 ± 1.45 P at%, and 2.55 ± 0.22 Ca/P ratio). Er:YAG laser irradiation alone or in combined protocols increased the Ca and P content of dental enamel, in vitro

Use of Hydrogels to Regulate Orthodontic Tooth Movement in Animal Models: A Systematic Review

The objective of this article is to conduct a systematic review of the literature to contrast the existing evidence regarding the use of hydrogels during and after experimental orthodontic treatment in animals. An extensive search was performed through the electronic databases, Medline, Web of Science and Scopus, from December 2020 to April 2021 for in vivo animal studies. A total of 282 studies were reviewed. Eight studies were included for final revision; four studies were conducted in rats, two in rabbits, one study in mice and one study in guinea pigs. The quality assessment of the eight included studies was performed according to the ARRIVE guidelines and the risk of bias was assessed using the Center for Systematic Review of Laboratory Animal Experimentation tool; in four of the eight articles evaluated, a high risk-of-bias rating was obtained in 40% of the criteria evaluated. In the studies reviewed, the hydrogel acted as a carrier, and inhibition (post-treatment retention) or acceleration of orthodontic tooth movement was assessed according to the active substance used in each of the articles. The uses of hydrogels for transporting active substances to regulate the rate of orthodontic tooth movement remains debatable. Future studies are suggested to evaluate the feasibility of hydrogel as a transport method in humans

Poly(3-hydroxybutyrate) graft copolymer dense membranes for human mesenchymal stem cell growth

Background: The use of novel materials as an artificial extracellular matrix for stem cell growth is a current strategy of increasing interest for regenerative medicine. Here, we prepare thermal-remolded membrane scaffolds from poly(3-hydroxybutyrate) grafted with 2-amino-ethyl methacrylate hydrochloride. However, it is unclear whether these membranes are useful for tissue engineering. Results: The mechanical properties, tribology, and morphology of the dense membranes were assessed. The results show that tensile strain at break and roughness of the compressed membrane decrease with increasing graft degree. Moreover, graft copolymer membranes showed lower resistance to scratching, greater degree of swelling and higher brittleness than un-grafted P(3HB) films. Thus, it effectively supports the growth of dermal fibroblast, as demonstrated by epifluorescence microscopy. Conclusions: It is concluded that the developed membrane can be properly used in is the restoration of skin tissue.How to cite: González-Torres M, Sánchez-Sánchez R, Solís-Rosales SG, et al. Poly(3-hydroxybutyrate) graft copolymer dense membranes for human mesenchymal stem cell growth. Electron J Biotechnol 2018, 34; https://doi.org/10.1016/j.ejbt.2018.05.007. Keywords: Artificial extracellular matrix, Dense membranes, Membrane scaffolds, Mesenchymal, Methacrylate, Regenerative medicine, Scaffolds, Stem cell growth, Stem cell growth, Thermal properties, Thermal-remolde