Genetic Divergence Among Accessions Of Cassava (manihot Esculenta Crantz) Sampled In The Tapajós Region, State Of Pará, Using Agronomic Characters And Microsatellite Markers

The aim of this work was to estimate the genetic divergence among accessions of cassava sampled in the Tapajós region in the State of Pará, Brazil, and conserved at the Regional Germplasm Bank of Eastern Amazon, using agronomic descriptors and molecular markers. Twenty-two accessions of cassava were evaluated in the field for two successive years, based on six agronomic descriptors in twelve-months-old plants without a specific experimental design. Accessions were also evaluated with eleven microsatellite loci in an automatic DNA analyser. Descriptive and multivariate statistical analyses were applied. Based on principal components analysis, the character weight of the aerial portion of the plant contributed most to the phenotypical variation. The six traits were used in the analysis of genetic dissimilarity between accessions, and the correlation between matrices generated by morphological and molecular data was estimated. The matrices of genetic dissimilarity were used in the construction of dendrograms using the UPGMA method. We observed a high variation of agronomical descriptors and molecular markers evaluated, which were capable to separate the accessions into distinct groups. A weak positive correlation was detected among the two matrices of genetic distances, which indicates the possibility to explore the genetic diversity using crossings and accessions Amarelinha 36 and Olho roxo 13 are divergent and potentially promising for the generation of heterotic hybrids.3752989300

Membrane-associated proteins of ejaculated sperm from Morada Nova rams

AbstractThe objective was to describe the profile of membrane proteins from sperm of tropically adapted Morada Nova rams (N = 5). Samples from protein-enriched fractions of ejaculated sperm (containing 400 μg of protein) were separated by two-dimensional electrophoresis and respective maps analyzed using PDQuest software (version 7.3.0; Bio-Rad). Proteins were identified using tandem mass spectrometry. Also, membrane proteins were incubated with antibodies against binder of sperm protein (BSP) 1 and bodhesin 2 (Bdh-2), components of vesicular gland secretion. For membrane proteins of ejaculated sperm, an average of 133 ± 4.6 spots were detected per gel, of which, 107 spots were consistently present on all gels. Sixty-eight spots and 37 proteins were identified using mass spectrometry, corresponding to 71.6% of the intensity of all spots detected. Three major spots identified as ram seminal vesicle protein (RSVP) 14 represented approximately 30% of the intensity of all spots. Two of the most intense spots in the gel reacted against anti-BSP1, at 14 kDa. In addition, four low molecular weight spots reacted with anti-Bdh-2 antibodies. Proteins RSVP and Bdh-2 belong to the BSP and spermadhesin families, respectively, and were previously reported as major components of ram seminal proteins. Additional proteins identified in the sperm membrane two-dimensional maps included alpha-2-heparan sulfate-glycoprotein, plasma glutamate carboxypeptidase, arylsulfatase A, cathelicidin, heat shock protein 70 kDa, angiotensin-converting enzyme, leucine aminopeptidase, and clusterin. Some proteins were present as multiple isoforms, such as tubulin (12), alpha-2-heparan sulfate-glycoprotein (5), ATP synthase (5), Bdh-2 (4) and RSVP14 (3). Based on gene ontology analysis, the most common biological processes associated with the membrane proteins were cellular processes (34%), response to stimulus (14%), and metabolic processes (11%). Binding (37%) and catalytic activity (32%) corresponded to the most frequent molecular functions for those proteins. In conclusion, we identified a diverse cohort of components of membrane proteins in ram sperm. Major proteins previously reported in seminal plasma, such as RSVP14 and Bdh-2, were also extracted from sperm membranes. Knowledge of sperm proteins is crucial for elucidating mechanisms underlying their association with sperm function

Four dimensional "old minimal" N=2 supersymmetrization of R^4

We write in superspace the lagrangian containing the fourth power of the Weyl tensor in the "old minimal" d=4, N=2 supergravity, without local SO(2) symmetry. Using gauge completion, we analyze the lagrangian in components. We find out that the auxiliary fields which belong to the Weyl and compensating vector multiplets have derivative terms and therefore cannot be eliminated on-shell. Only the auxiliary fields which belong to the compensating nonlinear multiplet do not get derivatives and could still be eliminated; we check that this is possible in the leading terms of the lagrangian. We compare this result to the similar one of "old minimal" N=1 supergravity and we comment on possible generalizations to other versions of N=1,2 supergravity.Comment: 31 pages, no figures. Minor corrections. Details of the full calculation included as an appendix. Reference adde

Laser printing of silver-based micro-wires in ZrO2 substrate for smart implant applications

Smart implants are endowed with functions of sensing, actuating and control to solve problems that may arise during their use. The assembly of these functions along the implant surface is still a challenge. However, with the advent of 3D printing, it is possible to print on implants’ surface, communication channels or micro-antennas or even sensoric/actuating areas. Hence, a positive impact on the long-term performance of the implants (including hip, dental and knee) may be expected with the proposed approach. Despite titanium and Ti6Al4V titanium alloy are the standard choice for implants fabrication, 3Y-TZP (tetragonal 3% mol yttria-stabilized zirconia) has emerged as a ceramic material suitable to overcome titanium alloy problems, due to its numerous advantages. In this sense, this work is concerned with the ability of printing silver-based communication system in zirconia substrates by using laser technology. For this purpose, micro-cavities were created on ZrO2 substrate, where the silver powder was placed and sintered into them. Through the laser approach, silver-based wires with great quality and low resistivity values were achieved. The flexural strength results showed that the mechanical resistance of zirconia disks was affected by laser micro-wire printing, which decreased as the laser passage was performed. Based on the results, it is believed that the proposed approach seems to be effective for the manufacturing of implants with intrinsic capacities, useful for smart implant applications.This work has been supported by FCT (Fundação para a Ciência e Tecnologia - Portugal) in the scope of the projects UID/EEA/04436/ 2019 and NORTE-01-0145-FEDER-000018-HAMaBICo and Add.Additive_Manufacturing to Portuguese Industry_POCI-01-0247- FEDER-024533. Thank the CNPq (205791/2014-0) and CAPES for the financial support

The first IEEE workshop on the Future of Research Curation and Research Reproducibility

This report describes perspectives from the Workshop on the Future of Research Curation and Research Reproducibility that was collaboratively sponsored by the U.S. National Science Foundation (NSF) and IEEE (Institute of Electrical and Electronics Engineers) in November 2016. The workshop brought together stakeholders including researchers, funders, and notably, leading science, technology, engineering, and mathematics (STEM) publishers. The overarching objective was a deep dive into new kinds of research products and how the costs of creation and curation of these products can be sustainably borne by the agencies, publishers, and researcher communities that were represented by workshop participants.National Science Foundation Award #164101

Eficiência do protocolo Ovsynch em ovelhas da raça Santa Inês

Twenty six Santa Inês ewes were asigned to three treatments to evaluate the efficiency of the Ovsynch protocol. In the treatment 1 - control (n= 8), the estrus was synchronized with sponges containing 60 mg MAP for 14 days. On D14, 300 IU eCG were administered. In treatment 2 (n= 9) the Ovsynch protocol was used: 25 µg of GnRH (D0), 37.5 µg of PGF2a (D7) and 25 µg of GnRH (D9). In treatment 3 (n= 9) the modified Ovsynch protocol was used: the administration of PGF2a and second GnRH as two days early. Estrus detection was accomplished using teaser. All ewes were mated twice with 12 hours of interval. Pregnancy rate (PR) was evaluated by ultrasonography 30 days after the end of mating. Estrus response was of 88.46% on average, and without differences among treatments (p>0.05). The interval for onset of estrus was greater (p0.05). Pregnancy rate was significantly greater (p0,05). O intervalo entre o final do tratamento e o início do estro foi maior (p0,05) entre tratamentos. A TP diferiu entre os grupos (37,5%, 62,5% e 25%, respectivamente. p<0,05). Os resultados mostram eficiência superior do protocolo Ovsynch, sob as restantes condições expe-rimentais

Hypotensive effect and endothelium-dependent vascular action of leaves of Alpinia purpurata (Vieill) K. Schum

The aims of this study were to evaluate the chemical profile, vascular reactivity, and acute hypotensive effect (AHE) of the ethanolic extract of leaves of Alpinia purpurata (Vieill) K. Schum (EEAP). Its chemical profile was evaluated using HPLC-UV, ICP-OES, and colorimetric quantification of total flavonoids and polyphenols. The vascular reactivity of the extract was determined using the mesenteric bed isolated from WKY. AHE dose-response curves were obtained for both EEAP and inorganic material isolated from AP (IAP) in WKY and SHR animals. Cytotoxic and mutagenic safety levels were determined by the micronucleus test. Rutin-like flavonoids were quantified in the EEAP (1.8 ± 0.03%), and the total flavonoid and polyphenol ratios were 4.1 ± 1.8% and 5.1 ± 0.3%, respectively. We observed that the vasodilation action of EEAP was partially mediated by nitric oxide (·NO). The IAP showed the presence of calcium (137.76 ± 4.08 μg mg-1). The EEAP and IAP showed an AHE in WKY and SHR animals. EEAP did not have cytotoxic effects or cause chromosomic alterations. The AHE shown by EEAP could result from its endothelium-dependent vascular action. Rutin-like flavonoids, among other polyphenols, could contribute to these biological activities, and the calcium present in EEAP could act in a synergistic way

Baroreflex sensitivity differs among same strain Wistar rats from the same laboratory

Previous studies showed that a proportion of normotensive Sprague-Dawley rats spontaneously exhibit lower baroreflex sensitivity. However, investigations have not yet been carried out on Wistar rats. We aimed to compare baroreflex sensitivity among rats from the same strain and the same laboratory. Male Wistar normotensive rats (300–400g) were studied. Cannulas were inserted into the abdominal aortic artery through the right femoral artery to measure mean arterial pressure and heart rate. Baroreflex was calculated as the derivative of the variation of heart rate in function of the mean arterial pressure variation (ΔHR/ΔMAP) tested with a depressor dose of sodium nitroprusside (50 µg/kg) and with a pressor dose of phenylephrine (8µg/kg) in the right femoral venous approach through an inserted cannula. We divided the rats into four groups: i) high bradycardic baroreflex, baroreflex gain less than −2 tested with phenylephrine; ii) low bradycardic baroreflex, baroreflex gain between −1 and −2 tested with phenylephrine; iii) high tachycardic baroreflex, baroreflex gain less than −3 tested with sodium nitroprusside; and iv) low tachycardic baroreflex, baroreflex gain between −1 and −3 tested with sodium nitroprusside. Approximately 71% of the rats presented a decrease in bradycardic reflex while around half showed an increase in tachycardic reflex. No significant changes in basal mean arterial pressure and heart rate, tachycardic and bradycardic peak and heart rate range were observed. There was a significant change in baroreflex sensitivity among rats from the same strain and the same laboratory