487 research outputs found
Locking the β\u3csub\u3e3\u3c/sub\u3e Integrin I-like Domain into High and Low Affinity Conformations with Disulfides
Although integrin α subunit I domains exist in multiple conformations, it is controversial whether integrin β subunit I-like domains undergo structurally analogous movements of the α7-helix that are linked to affinity for ligand. Disulfide bonds were introduced into the β3 integrin I-like domain to lock its β6-α7 loop and α7-helix in two distinct conformations. Soluble ligand binding, ligand mimetic mAb binding and cell adhesion studies showed that disulfide-bonded receptor αIIbβ3T329C/A347C was locked in a low affinity state, and dithiothreitol treatment restored the capability of being activated to high affinity binding; by contrast, disulfide-bonded αIIbβ3V332C/M335C was locked in a high affinity state. The results suggest that activation of the β subunit I-like domain is analogous to that of the α subunit I domain, i.e. that axial movement in the C-terminal direction of the α7-helix is linked to rearrangement of the I-like domain metal ion-dependent adhesion site into a high affinity conformation
The distance of M33 and the stellar population in its outskirts
We present deep V,I photometry of two $9.4' x 9.4' field in the outer regions
of the M33 galaxy. We obtain a robust detection of the luminosity of the Red
Giant Branch Tip (I{TRGB}=20.72 +- 0.08) from which we derived a new estimate
of the distance modulus of M33, (m-M)_0=24.64 +- 0.15, corresponding to a
distance D=847 +- 60 Kpc. By comparison of the color and magnitude of the
observed Red Giant Branch stars with ridge lines of template globular clusters
we obtained the photometric metallicity distribution of the considered fields
in three different metallicity scales. The derived metallicity distributions
are very similar over a range of distances from the galactic center 10' <= R <=
33', and are characterized by a well defined peak at [M/H] ~ -0.7 ([Fe/H] ~
-1.0, in the Zinn & West scale) and a weak metal-poor tail reaching [M/H] ~
-2.0. Our observations demonstrate that Red Giant Branch and Asymptotic Giant
Branch stars have a radial distribution that is much more extended than the
young MS stars associated with the star-forming disc.Comment: 10 pages,10 figures,accepted for publication in Astronomy &
Astrophysic
K Corrections For Type Ia Supernovae and a Test for Spatial Variation of the Hubble Constant
Cross-filter K corrections for a sample of "normal" Type Ia supernovae (SNe)
have been calculated for a range of epochs. With appropriate filter choices,
the combined statistical and systematic K correction dispersion of the full
sample lies within 0.05 mag for redshifts z<0.7. This narrow dispersion of the
calculated K correction allows the Type Ia to be used as a cosmological probe.
We use the K corrections with observations of seven SNe at redshifts 0.3 < z
<0.5 to bound the possible difference between the locally measured Hubble
constant (H_L) and the true cosmological Hubble constant (H_0).Comment: 6 pages, 3 Postscript figures, uuencoded uses crckapb.sty and
psfig.sty. To appear in Thermonuclear Supernovae (NATO ASI), eds. R. Canal,
P. Ruiz-LaPuente, and J. Isern. Postscript version is also available at
http://www-supernova.lbl.gov
A Database of Cepheid Distance Moduli and TRGB, GCLF, PNLF and SBF Data Useful for Distance Determinations
We present a compilation of Cepheid distance moduli and data for four
secondary distance indicators that employ stars in the old stellar populations:
the planetary nebula luminosity function (PNLF), the globular cluster
luminosity function (GCLF), the tip of the red giant branch (TRGB), and the
surface brightness fluctuation (SBF) method. The database includes all data
published as of July 15, 1999. The main strength of this compilation resides in
all data being on a consistent and homogeneous system: all Cepheid distances
are derived using the same calibration of the period-luminosity relation, the
treatment of errors is consistent for all indicators, measurements which are
not considered reliable are excluded. As such, the database is ideal for
inter-comparing any of the distance indicators considered, or for deriving a
Cepheid calibration to any secondary distance indicator. Specifically, the
database includes: 1) Cepheid distances, extinctions and metallicities; 2)
apparent magnitudes of the PNLF cutoff; 3) apparent magnitudes and colors of
the turnover of the GCLF (both in the V- and B-bands); 4) apparent magnitudes
of the TRGB (in the I-band) and V-I colors at and 0.5 magnitudes fainter than
the TRGB; 5) apparent surface brightness fluctuation magnitudes I, K', K_short,
and using the F814W filter with the HST/WFPC2. In addition, for every galaxy in
the database we give reddening estimates from DIRBE/IRAS as well as HI maps,
J2000 coordinates, Hubble and T-type morphological classification, apparent
total magnitude in B, and systemic velocity. (Abridged)Comment: Accepted for publication in the Astrophysical Journal Supplement
Series. Because of space limitations, the figures included are low resolution
bitmap images. Original figures can be found at
http://www.astro.ucla.edu/~laura/pub.ht
The HST Key Project on the Extragalactic Distance Scale XXV. A Recalibration of Cepheid Distances to Type Ia Supernovae and the Value of the Hubble Constant
Cepheid-based distances to seven Type Ia supernovae (SNe)-host galaxies have
been derived using the standard HST Key Project on the Extragalactic Distance
Scale pipeline. For the first time, this allows for a transparent comparison of
data accumulated as part of three different HST projects, the Key Project, the
Sandage et al. Type Ia SNe program, and the Tanvir et al. Leo I Group study.
Re-analyzing the Tanvir et al. galaxy and six Sandage et al. galaxies we find a
mean (weighted) offset in true distance moduli of 0.12+/-0.07 mag -- i.e., 6%
in linear distance -- in the sense of reducing the distance scale, or
increasing H0. Adopting the reddening-corrected Hubble relations of Suntzeff et
al. (1999), tied to a zero point based upon SNe~1990N, 1981B, 1998bu, 1989B,
1972E and 1960F and the photometric calibration of Hill et al. (1998), leads to
a Hubble constant of H0=68+/-2(random)+/-5(systematic) km/s/Mpc. Adopting the
Kennicutt et al. (1998) Cepheid period-luminosity-metallicity dependency
decreases the inferred H0 by 4%. The H0 result from Type Ia SNe is now in good
agreement, to within their respective uncertainties, with that from the
Tully-Fisher and surface brightness fluctuation relations.Comment: Accepted for publication in The Astrophysical Journal. 62 pages,
LaTeX, 9 Postscript figures. Also available at
http://casa.colorado.edu/~bgibson/publications.htm
The HST Key Project on the Extragalactic Distance Scale. XXVIII. Combining the Constraints on the Hubble Constant
Since the launch of the Hubble Space Telescope nine years ago, Cepheid
distances to 25 galaxies have been determined for the purpose of calibrating
secondary distance indicators. A variety of these can now be calibrated, and
the accompanying papers by Sakai, Kelson, Ferrarese, and Gibson employ the full
set of 25 galaxies to consider the Tully-Fisher relation, the fundamental plane
of elliptical galaxies, Type Ia supernovae, and surface brightness
fluctuations.
When calibrated with Cepheid distances, each of these methods yields a
measurement of the Hubble constant and a corresponding measurement uncertainty.
We combine these measurements in this paper, together with a model of the
velocity field, to yield the best available estimate of the value of H_0 within
the range of these secondary distance indicators and its uncertainty.
The result is H_0 = 71 +/- 6 km/sec/Mpc. The largest contributor to the
uncertainty of this 67% confidence level result is the distance of the Large
Magellanic Cloud, which has been assumed to be 50 +/- 3 kpc
The Extragalactic Distance Scale Key Project XXVII. A Derivation of the Hubble Constant Using the Fundamental Plane and Dn-Sigma Relations in Leo I, Virgo, and Fornax
Using published photometry and spectroscopy, we construct the fundamental
plane and D_n-Sigma relations in Leo I, Virgo and Fornax. The published Cepheid
P-L relations to spirals in these clusters fixes the relation between angular
size and metric distance for both the fundamental plane and D_n-Sigma
relations. Using the locally calibrated fundamental plane, we infer distances
to a sample of clusters with a mean redshift of cz \approx 6000 \kms, and
derive a value of H_0=78+- 5+- 9 km/s/Mpc (random, systematic) for the local
expansion rate. This value includes a correction for depth effects in the
Cepheid distances to the nearby clusters, which decreased the deduced value of
the expansion rate by 5% +- 5%. If one further adopts the metallicity
correction to the Cepheid PL relation, as derived by the Key Project, the value
of the Hubble constant would decrease by a further 6%+- 4%. These two sources
of systematic error, when combined with a +- 6% error due to the uncertainty in
the distance to the Large Magellanic Cloud, a +- 4% error due to uncertainties
in the WFPC2 calibration, and several small sources of uncertainty in the
fundamental plane analysis, combine to yield a total systematic uncertainty of
+- 11%. We find that the values obtained using either the CMB, or a flow-field
model, for the reference frame of the distant clusters, agree to within 1%. The
Dn-Sigma relation also produces similar results, as expected from the
correlated nature of the two scaling relations. A complete discussion of the
sources of random and systematic error in this determination of the Hubble
constant is also given, in order to facilitate comparison with the other
secondary indicators being used by the Key Project.Comment: 21 pages, 3 figures, Accepted for publication in Ap
Mycoplasma pneumoniae Infection Affects the Serum Levels of Vascular Endothelial Growth Factor and Interleukin-5 in Atopic Children
Purpose: Previous studies have outlined mechanisms by which Mycoplasma pneumonia (M. pneumonia) infection may promote allergic lung inflammation and airway remodeling, and increasing evidence from human studies suggests that atypical bacterial infections contribute to asthma exacerbation,chronic asthma, and disease severity with changes in cytokine expression. The present study evaluated changes in serum levels of vascular endothelial growth factor (VEGF) and interleukin (IL)-5 in atopic children with Mycoplasma pneumoniae pneumonia. Methods: We recruited a total of 72 children with pneumonia. The patients were divided into 4 groups: atopic children with M. pneumonia pneumonia (group I, n=24), nonatopic children with M. pneumonia pneumonia (group II, n=23), atopic children with viral pneumonia (group III, n=13), and non-atopic children with viral pneumonia (group IV, n=12). Serum levels of IL-5, IL-13, VEGF, and tumor necrosis factor-α were measured at admission and at recovery using enzyme-linked immunosorbent assays. Results: Serum levels of VEGF and IL-5 were elevated in group I compared with the other groups at both admission phase and clinical recovery phase. In group I, serum levels of VEGF and IL-5 were higher at recovery phase than at admission phase (VEGF:1,102.2±569.4 vs. 874.9±589.9 pg/mL, respectively; IL-5: 150.5±63.9 vs. 120.2±46.7 pg/mL, respectively). Conclusions: The serum levels of VEGF and IL-5 were more increased in atopic children with M. pneumonia pneumonia than in the other groups. In this group, the serum levels of VEGF and IL-5 were more increased at recovery phase than at admission phase. The results of this study suggest that increases in VEGF and IL-5 may contribute to the development of hypersensitivity during M. pneumonia infection. These cytokines may act through their respective pro-inflammatory pathways to aggravate the allergic status and induce airway hypersensitivity during M. pneumonia pneumonia in atopic children.This study was supported by the 2010 Hanyang University, College of Medicine, Research Fund (HY-2010-MC) and a Research Grant of Alumni of Pediatrics, College of Medicine, Hanyang University
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