Multiplexed Ultrasensitive Determination of Adrenocorticotropin and Cortisol Hormones at a Dual Electrochemical Immunosensor

A novel dual electrochemical immunosensor for the multiplexed determination of adrenocorticotropin (ACTH) and cortisol is reported. Aminophenylboronic acid-modified dual screen-printed carbon electrodes were prepared on which the corresponding ACTH and cortisol antibodies were immobilized. Competitive immunoassays involved biotinylated ACTH and alkaline phosphatase labelled streptavidin, or alkaline phosphatase labelled cortisol. Differential pulse voltammetry upon 1-naphtyl phosphate addition was employed to monitor the affinity reactions. The ranges of linearity were 5.0×10−5−0.1 and 0.1−500 ng/mL for ACTH and cortisol. The usefulness of the dual immunosensor was demonstrated by analyzing certified human serum samples with good recoveries

Synthesis, properties, and some rhodium, iridium, and platinum complexes of a series of bulky m-terphenylphosphine ligands

A series of sterically demanding aryl phosphine ligands (L) bearing terphenyl substituents, PR2Ar′ (R = hydrocarbyl, Ar′ = 2,6-diarylphenyl radical) has been prepared and characterized. The stability of these ligands towards oxidation in the air has been tested, and theoretical and experimental studies aimed to provide information on their electronic and steric properties have been performed. Treatment of the metal dimers [MCl(COD)]2(M = Rh, Ir; COD = 1,5-cyclooctadiene) with ligands PMe2ArXyl2(L1) and PMe2ArDipp2(L5), in a 1:1 metal:ligand ratio, afforded the expected square-planar 16-electron complexes [MCl(COD)(PMe2ArXyl2)] and [MCl(COD)(PMe2ArDipp2)], respectively. These compounds were readily converted into the corresponding dicarbonyl derivatives, [MCl(CO)2(PMe2ArXyl2)] and [MCl(CO)2(PMe2ArDipp2)], respectively. While the expected κ1-P coordination mode of the PR2Ar′ ligands is found for these rhodium and iridium species, the mononuclear Pt(II) derivative obtained by reaction of PtCl2with PMe2ArDipp2has composition [PtCl2(PMe2ArDipp2)], and exhibits a bidentate κ1-P, η1-arene coordination mode involving one of the ipso carbon atoms of a flanking terphenyl aryl ring. The corresponding carbonyl compound [PtCl2(CO)(PMe2ArDipp2)], was generated under a CO atmosphere and exhibits κ1-P coordination mode.Ministerio de Ciencia CTQ2013-42501-P, CTQ2014-51912-REDC, CTQ-2014-52769-C3-3-RJunta de Andalucía FQM-119 P09-FQM-4832, FQM-2126European Union 26722

Evaluating the creative potencial of digital storytelling APPs for Primary Education

This study evaluates the creative potential of different apps (N=20) oriented to the de-sign of digital storytelling (DST), directed to users from 6 to 12 years, using the CREAPP K6-12 instrument, made of 48 indicators related to the 5 dimensions of creativity: flexibility, originality, fluency, product elaboration and problem solving, co-edition and dissemination. After adopting descriptive and multivariate statistical techniques, the results show that 76.6% of these apps have great flexibility, 73.4% favor originality, 72.4% present resources and function-alities endowing them with great fluidity, 68.3% encourage the creation of creative products, 56.5% promote the resolution of problems, yet only 36.3% prevail the co-edition and dissemination of stories in networks. The correlations show that the most flexible app stimulates originality and fluency, propitiates problem solving and promotes the elaboration of creative products. The index of creative potentiality of the apps is calculated, establishing a competitiveness ranking, which offers keys to the Primary teachers to elaborate didactic proposals that promote creativity through the design of DST

Familia y escuela en el desarrollo corporal de adolescentes de los ciclos 4 y 5 de cuatro colegios distritales, en el tema de modificaciones corporales

Tesis (Maestría en Docencia). -- Universidad de la Salle. Facultad de Ciencias de la Educación. Maestría en Docencia, 201

Diseño y optimización de un medio de cultivo a base de melaza de caña para la producción de biomasa a partir de saccharomyces cerevisiae

Objetivo: Diseñar y optimizar un medio a base de melaza de caña suplementado con macronutrienes y micronutrientes que favorezcan la producción de biomasa de Saccharomyces cerevisiae. Metodología: La cepa Saccharomyces cerevisiae INRA fue reconstituida en agar YPG y se conservó en glicerol 20% (v/v). Posteriormente se realizó una cinética preliminar en caldo YPG para conocer el comportamiento de la levadura y realizar una comparación con los resultados obtenidos en los medios a base de melaza de caña. Seguido a esto, se realizó un ensayo enzimático a diferentes concentraciones de invertasa y tiempos de acción para cuantificar los azúcares reductores presentes en la melaza. Luego se realizaron 3 cinéticas en solución de melaza al 10% (p/v), 20% (p/v) y 40% (p/v) para determinar la concentración a la cual se obtenía mayor producción de biomasa en melaza pura. A continuación se realizó el montaje del diseño Plackett Burman (screening primario) con 9 factores: extracto de levadura, sulfato de amonio, fosfato monobásico de potasio, fosfato dibásico de potasio, sulfato de magnesio, cloruro de calcio, cloruro de zinc, tiamina y ácido pantoténico, para determinar la influencia que tiene cada uno de estos sobre la producción de biomasa. Finalmente se realizó un screening secundario comparando el mejor medio con una solución de melaza pura para determinar diferencias en cuanto a la producción de biomasa y demás parámetros medidos. Resultados: De acuerdo al análisis estadístico ANOVA (p menor que 0.05) realizado a los datos obtenidos en el Plackett no existe evidencia estadísticamente significativa para afirmar que algún componente nutricional está afectando directamente la producción de biomasa. Con respecto al screening secundario, no se observaron diferencias estadísticamente significativas en cuanto a la producción de biomasa, sin embargo se observó una diferencia en el pH pero que no afectó la producción y viabilidad celular. Conclusiones: La melaza pura a una concentración de 20% (p/v) representa una buena fuente nutricional para el crecimiento de Saccharomyces cerevisiae ya que cuenta con la mayoría de los macroelementos esenciales para el desarrollo de este microorganismo.Microbiólogo (a) IndustrialPregrad

Familia y escuela en el desarrollo corporal de adolescentes de los ciclos 4 y 5 de cuatro colegios distritales, en el tema de modificaciones corporales

174 p. Libro Electronicoprimera edicionPublicación resultado del programa INCENTIVA, una apuesta para generar un sistema de estímulos y reconocimientos a redes, colectivos, semilleros de investigación y docentes investigadores e innovadore

Magnetic reduced graphene oxide/nickel/platinum nanoparticles micromotors for mycotoxin analysis

Magnetic reduced graphene oxide/nickel/platinum nanoparticles (rGO/Ni/PtNPs) micromotors for mycotoxin analysis in food samples were developed for food-safety diagnosis. While the utilization of self-propelled micromotors in bioassays has led to a fundamentally new approach, mainly due to the greatly enhanced target?receptor contacts owing to their continuous movement around the sample and the associated mixing effect, herein the magnetic properties of rGO/Ni/PtNPs micromotors for mycotoxin analysis are additionally explored. The micromotor-based strategy for targeted mycotoxin biosensing focused on the accurate control of micromotor-based operations: 1) on-the-move capture of free aptamers by exploiting the adsorption (outer rGO layer) and catalytic (inner PtNPs layer) properties and 2) micromotor stopped flow in just 2 min by exploiting the magnetic properties (intermediate Ni layer). This strategy allowed fumonisin B1 determination with high sensitivity (limit of detection: 0.70 ngmL@1) and excellent accuracy (error: 0.05% in certified reference material and quantitative recoveries of 104:4% in beer) even in the presence of concurrent ochratoxin A (105?108:8% in wines). These results confirm the developed approach as an innovative and reliable analytical tool for food-safety monitoring, and confirm the role of micromotors as a new paradigm in analytical chemistry.Ministerio de Economía y CompetitividadComunidad de Madri