Frequencies of deletereus character in the spanish water dog affecting the breed conservation

321 puppies of the Spanish Water Dog Breed have been used to study the frequency of presentation of several deleterious characters between the years 1995 and 1998. The enogmatism and the monorquidism shown frequencies relatively low, but we stand out these characters because their control is very important for the relationship with respect the adaptive capacity of these animals. In the same way the prognatism and the absence of premolars were present with a frequency slightly high, it must take into account in the selection criteria of the breed. By another side, we found a signifficative association between the presence of heath anomalies and slightly high populational levels of inbreeding, and then we have to avoid these breeding practices in the racial lines presenting this defect.Sobre un total de 321 cachorros nacidos de la raza Perro de Agua Español entre los años 1995 y 1998 se estudian las frecuencias de aparición de diferentes caracteres deletéreos. Los caracteres enogmatismo y monorquidia muestran unas frecuencias relativamente bajas, si bien destacamos la importancia de su control ya que su expresión afecta a la capacidad adaptativa de los animales. Asimismo, el prognatismo y la ausencia de premolares se presentan con unas frecuencias moderadamente altas que recomendamos incluir en los criterios de selección de la raza. Por otra parte, encontramos asociación significativa entre la aparición de anomalías cardíacas y niveles relativamente altos de endogamia en la población por lo que se desaconseja la endocría en las líneas raciales que presentan este defecto

Evaluation of a coupled hydrodynamic-closed ecological cycle approach for modelling dissolved oxygen in surface waters

The description of intertwined ecological processes in surface waters requires a holistic approach that accounts for spatially distributed hydrological/water quality processes. This study describes a new approach to model dissolved oxygen (DO) based on linked hydrodynamic and closed nutrient cycle ecological models. Long term datasets from the River Dommel (Netherlands) are used to determine: 1) if this methodology is suitable for modelling DO concentrations, 2) the model sensitivity to various levels of nutrients input, and 3) the DO production and consumption processes and their response to nutrient input changes. Results show that seasonal dynamics of DO are well quantified at long timescales; the sensitivity of DO to different pollutant sources exhibits significant seasonal variation and the largest influences on DO are aeration and mineralization of organic material. The approach demonstrates an ability to consider the impacts of nutrient input and long term vegetation maintenance on ecological quality

Production of structured lipids by acidolysis of an EPA-enriched fish oil and caprylic acid in a packed bed reactor: analysis of three different operation modes.

Structured triacylglycerols (ST) enriched in eicosapentaenoic acid (EPA) in position 2 of the triacylglycerol (TAG) backbone were synthesized by acidolysis of a commercially available EPA-rich oil (EPAX4510, 40% EPA) and caprylic acid (CA), catalyzed by the 1,3-specific immobilized lipase Lipozyme IM. The reaction was carried out in a packed bed reactor (PBR) operating in two ways: (1) by recirculating the reaction mixture from the exit of the bed to the substrate reservoir (discontinuous mode) and (2) in continuous mode, directing the product mixture leaving the PBR to a product reservoir. By operating in these two ways and using a simple kinetic model, representative values for the apparent kinetic constants (k X ) for each fatty acid (native, L i or odd, M) were obtained. The kinetic model assumes that the rate of incorporation of a fatty acid into TAG per amount of enzyme, r X (mole/(h g lipase)) is proportional to the extent of the deviation from the equilibrium for each fatty acid (i.e., the difference of concentration between the fatty acid in the triacylglycerol and the concentration of the same fatty acid in the triacylglycerol once the equilibrium of the acidolysis reaction is reached). The model allows comparing the two operating modes through the processing intensity, defined as m L t/(V[TG] 0 ) and m L /(q[TG] 0 ), for the discontinuous and continuous operation modes, respectively. In discontinuous mode, ST with 59.5% CA and 9.6% EPA were obtained. In contrast, a ST with 51% CA and 19.6% EPA were obtained when using the continuous operation mode. To enhance the CA incorporation when operating in continuous mode, a two-step acidolysis reaction was performed (third operation mode). This continuous two-step process yields a ST with a 64% CA and a 15% EPA. Finally, after purifying the above ST in a preparative silica gel column, impregnated with boric acid, a ST with 66.9% CA and 19.6% EPA was obtained. The analysis by reverse phase and Ag + liquid chromatography of the EPA-enriched ST demonstrated that the CA was placed in positions 1 and 3 and the EPA was occupying position 2 of the final ST

Simulation-based optimization approach for vehicle allocation in a private transport service: A case study

Poor urban planning and traffic congestion lead to excessive delays in workers’ transit times and decrease their quality of life, especially in emerging countries. Several medium and large companies have a need to hire a transport service for their staff. In this type of transportation system, there is a heterogeneous fleet of vehicles, which are assigned to a set of predefined routes. However, the total transport delay can be even greater if the private transportation system is inefficient or not controlled. The approach proposed in this study seeks to optimize the private transport service by defining the best allocation of its fleet to its routes. A mathematical model is proposed to minimize user wait times. This approach is validated using real data obtained from a transport company in Co-lombia. The results demonstrate the quantitative benefits that can be achieved when the proposed approach is implemented, represented by a considerable reduction in user wait times

Resolución de problemas científicos escolares y promoción de competencias de pensamiento científico. ¿Qué piensan los docentes de química en ejercicio?

En el artículo se exponen los resultados de una investigación realizada en el marco del proyecto FONDECYT 1070795, cuyo objetivo consistió en identificar y caracterizar las nociones que tienen los profesores de nivel medio respecto a las competencias de pensamiento científico y solución de problemas, y la manera en que ambos deben trabajarse en la enseñanza de la química. Con el empleo de una muestra intencional de 33 profesores, seleccionados de un total de 117 que se incorporaron a otras fases de la investigación y mediante la administración de un cuestionario especialmente diseñado, se logró poner de manifiesto que en ellos predomina una imagen fragmentada y a veces contradictoria de la solución de problemas y de las competencias de pensamiento científico; dicha representación incorpora aspectos de inestimable valor para la formación del estudiante competente, a la vez que incluye otros que en manera alguna favorecen dicha formación en correspondencia con las exigencias actuales. Es notable que competencias de pensamiento científico y solución de problemas no se constituyan como sistema totalmente coherente en la conceptualización de los profesores investigados. Como dato relevante, se constató la oscilante valoración del carácter subjetivo de las situaciones problémicas y los problemas; junto al papel importante que, en opinión de gran parte de los profesores, desempeña el lenguaje, se le otorga un valor oscilante al trabajo con la teoría por parte de los estudiantes; igualmente resulta difusa, y a veces contradictoria, su aproximación a los procesos de algoritmización.In the article the results of an investigation conducted within the framework of the project FONDECYT 1070795 are exposed. The aims of the research were to identify and characterize the representations and knowledge that teachers in service have, from an average level of education, with respect to the competences of scientific thought and problem solution, and the way in which both should be approached. With the use of an intentional sample of 133, teachers selected from a total of 117 who were incorporated to other phases of the research trough the administration of a questionnaire especially designed, was show the predominance of a very fragmented image, sometimes contradictory, about the solution of problems and about relation between competences of scientific thought; this representation incorporates aspects of great value for the formation of the competent students, and at the same time it includes other aspects that in no way favor this formation, according to the present assessments. It is remarkable that both competences for scientific thought and solution of problems are not constituted as a totality and coherent system in the thinking of the teachers who participated in the research. It should be pointed out that we found data that show an oscillating valuation of the subjective character of the problematic situations and the problem itself; these data show, as well, that teachers give a very important role to language. It is stated that teachers give an oscillating value to the fact that students must deal with theories. It should be noted too that their approaches to the algorithmic processes are very vague, and sometimes contradictory

Distribución espacial de la fructificación del hongo ECM comestible Amanita ponderosa Malençon & R. Heim durante seis años consecutivos en un encinar adehesado de la Sierra de Aracena (Huelva)

Number and location of Amanita ponderosa Malençon & R. Heim sporocarps was followed through six years in a 3 ha holm-oak forest located in the Sierra de Aracena y Picos de Aroche Natural Park (Huelva), at the Southwest of Spain. The data indicated that patches of sporocarp production showed a very similar shape each year, but some spots showed differences seeming crop alternance or mast years. This species only fructifies in spring, being the fruiting period from 6 to 8 weeks, what coincides with the increasing of air-temperature from mid February to April. Differences in fruiting period length and total sporocarp production are related to rainfall and temperature. Results suggest that rainfalls at the end of summer and early autumn are crucial for the next spring fruiting, but rainfalls during fruiting period seem to have less influence in sporocarp production. During the sporocarp production period, it was related to soil temperature.Se ha estudiado durante seis años consecutivos el número y la localización de los esporocarpos de Amanita ponderosa Malençon & R. Heim en una parcela de 3 ha ubicada en un encinar adehesado localizado en el Parque Natural «Sierra de Aracena y Picos de Aroche» (Huelva). Los datos indican que las áreas productoras de setas presentan un tamaño y una forma similares año tras año, aunque algunas zonas específicas muestran variaciones que sugieren la existencia de cierta alternancia o vecería. Esta especie fructifica solamente en primavera durante seis a ocho semanas, desde mediados de febrero hasta abril, cuando comienza a ascender la temperatura media tras el invierno. Las diferencias observadas en la duración del periodo de fructificación y la producción total de setas dependen fundamentalmente de las lluvias y la temperatura. Los resultados sugieren que las lluvias tempranas a finales de verano o principios de otoño son cruciales para la fructificación de la primavera siguiente, y que las lluvias caídas durante el periodo de fructificación tienen una menor influencia. Durante el periodo de fructificación, la producción es muy dependiente de la temperatura del suelo

Fungal microbiota from rain water and pathogenicity of Fusarium species isolated from atmospheric dust and rainfall dust

In order to determine the presence of Fusarium spp. in atmospheric dust and rainfall dust, samples were collected during September 2007, and July, August, and October 2008. The results reveal the prevalence of airborne Fusarium species coming from the atmosphere of the South East coast of Spain. Five different Fusarium species were isolated from the settling dust: Fusarium oxysporum, F. solani, F. equiseti, F. dimerum, and F. proliferatum. Moreover, rainwater samples were obtained during significant rainfall events in January and February 2009. Using the dilution-plate method, 12 fungal genera were identified from these rainwater samples. Specific analyses of the rainwater revealed the presence of three species of Fusarium: F. oxysporum, F. proliferatum and F. equiseti. A total of 57 isolates of Fusarium spp. obtained from both rainwater and atmospheric rainfall dust sampling were inoculated onto melon (Cucumis melo L.) cv. Piñonet and tomato (Lycopersicon esculentum Mill.) cv. San Pedro. These species were chosen because they are the main herbaceous crops in Almeria province. The results presented in this work indicate strongly that spores or propagules of Fusarium are able to cross the continental barrier carried by winds from the Sahara (Africa) to crop or coastal lands in Europe. Results show differences in the pathogenicity of the isolates tested. Both hosts showed root rot when inoculated with different species of Fusarium, although fresh weight measurements did not bring any information about the pathogenicity. The findings presented above are strong indications that long-distance transmission of Fusarium propagules may occur. Diseases caused by species of Fusarium are common in these areas. They were in the past, and are still today, a problem for greenhouses crops in Almería, and many species have been listed as pathogens on agricultural crops in this region. Saharan air masses dominate the Mediterranean regions. The evidence of long distance dispersal of Fusarium spp. by atmospheric dust and rainwater together with their proved pathogenicity must be taken into account in epidemiological studies

Colombian consensus on the diagnosis, treatment, and prevention of candida Spp. disease in children and adults

La Candidiasis Invasora (CI) y la candidemia, como su manifestación más frecuente, se ha convertido en la principal causa de micosis oportunista a nivel hospitalario. Este manuscrito realizado por miembros de la Asociación Colombiana de Infectología (ACIN), tuvo como objetivo proporcionar un conjunto de recomendaciones para manejo, seguimiento y prevención de la CI/candidemia y de la infección candidiásica de mucosas, en población adulta, pediátrica y neonatal, en un entorno hospitalario, incluyendo las unidades hemato-oncológicas y unidades de cuidado crítico. Todos los datos obtenidos mediante una búsqueda exhaustiva, fueron revisados y analizados de manera amplia por todos los miembros del grupo, y las recomendaciones emitidas se elaboraron luego de la evaluación de la literatura científica disponible, y el consenso de todos los especialistas involucrados, reconociendo el problema de la emergencia de las infecciones por Candida Spp. y brindando una correcta orientación a los profesionales de la salud sobre el manejo de pacientes con enfermedad candidiásica, de una forma racional y práctica, enfatizando en la evaluación del paciente, estrategias de diagnóstico, profilaxis, tratamiento empírico, tratamiento dirigido y terapia preventiva.Invasive Candidiasis (IC) and candidemia (as its most frequent manifestation) have become the main cause of opportunistic mycosis at hospital settings. This study, made by members of the Colombian Association of Infectious Diseases (ACIN), was aimed at providing a set of recommendations for the management, follow-up and prevention of IC / candidemia and mucous membrane candida infection in adult, pediatric and neonatal patients in a hospital setting, including the hemato-oncological and critical care units. All the data obtained through an exhaustive search were reviewed and analyzed in a comprehensive manner by all the members of the group, and the recommendations issued are being made after a careful review of the scientific literature available and the consensus of all specialists involved; the emergence of Candida Spp. problem is highlighted and a correct orientation to health professionals regarding the management of patients with candidiasis is provided in a rational and practical way, emphasizing patient evaluation, diagnostic strategies, prophylaxis, empirical treatment, directed treatment and preventative therap

The burden of 14 hr-HPV genotypes in women attending routine cervical cancer screening in 20 states of Mexico: a cross-sectional study

In Mexico, HPV vaccines available immunize against genotypes 16/18 and 16/18/6/11; however, there is limited surveillance about carcinogenic subtypes in different states of the country that allow evaluating the effectiveness of vaccination and cervical cancer screening programs. Here, we report the regional and age-specific prevalence of 14 hr-HPV genotypes as well as their prevalence in abnormal cytology (from ASCUS to cervical cancer) among Mexican women which were undergoing from cervical cancer screening in the Salud Digna clinics in 20 states of the country. This study includes women with social security from the majority of public health institutions (IMSS, ISSSTE, SEMAR, and PEMEX), and women without social security. For cervical cancer screening, we used the SurePath liquid-based cytology and the BD Onclarity HPV Assay. From December 1, 2016, to August 2, 2018, the hr-HPV prevalence among 60,135 women was 24.78%, the most prevalent types were HPV 16 (4.13%), HPV 31 (4.12%) and HPV 51 (3.39%), while HPV 18 (1.70%) was less prevalent among infected women. Interestingly, the genotypes not covered by current vaccines in Mexico were commonly found in precancerous lesions, evidencing their carcinogenic potential, so it is necessary to increase their surveillance and inclusion in cervical cancer screening triage.We gratefully acknowledge to Iromy Meza, Jessica Avitia, and Oswaldo Carrillo for their technical support in obtaining databases during this project. Also, we want to thanks the staff of the Salud Digna clinics and the National Reference Center of Salud Digna for their support during this work. This work was funding by Salud Digna