Phenolic Compounds and Antioxidant Activity of Md2 Pineapple (Ananas comosus L.) Variety at Three Stages of Ripening

MD2 pineapple fruits with different ripening stages (stage 1-green skin, stage 2- 50% yellowing of skin, and stage 3-100% yellowing of skin), were used to determine the total phenolic, total flavonoid content, and condensed tannins using Folin-Ciocalteu, aluminum chloride, and acid butanol assays, respectively. Assessment of the antioxidant activity of the pineapple flesh was performed using ABTS and DPPH radical assays. Total phenolic content, total flavonoids and condensed tannins of the flesh varied among samples with 160.7 to 197.4 mg GAE/ 100 g DW, 7.7 to 14.7 mg CE/ 100 g DW, and 77.1 to 124.2 mg tannin eq./ 100 g DW, respectively. In the peel, the total phenolics, total flavonoids, and condensed tannins showed significant differences among the samples as ripening stages increased. ABTS radical scavenging activity of the flesh samples showed weak correlation against total phenolic, total flavonoids, and condensed tannins but peel samples showed moderate correlation. DPPH radical scavenging activity of the flesh and peel samples showed strong positive correlation with total phenolics, total flavonoids, and condensed tannins. These results suggest that pineapple is a potential source of phytochemicals with antioxidant activities

Student Record Data Useful in Secondary School Curriculum Improvement for Health Education

The purpose of this study was to find recorded data on students\u27 records in a selected Seventh-day Adventist church-sponsored secondary school which could be of acceptable value in curriculum improvement in health education. A survey method of research was chosen with the study of two available records which were: (1) The Medical Examination Records and (2) the Mooney Problem Check List. The Medical Examination Records were filled out by students\u27 private physicians on their first admission to the school; the Mooney Problem Check List were administered by the school on October 24, 1961. These students were from grades nine through twelve and included both sexes whose ages ranged from thirteen to eighteen. The data gathered from the records were tabulated, analyzed and interpreted. The Medical Examination Records showed an apparently healthy adolescence student group with the execution of a small minority with possible remedial defects of the skin, vision, hearing, nose and throat. The three problem categories selected for study from the Mooney Problem Check List indicated varying degrees of deviation in mental and emotional behavior patterns. The outstanding problem that was most disturbing in this student group was found in the area of Social-Psychological Relations, which was, wanting a more pleasing personality. The next highest problem of concern was indicated in the Personal-Psychological Problem category as evidenced by a high response in Forgetting things. The problem of Health and Physical Development although mot as disturbing as the other two problem categories was relatively high which was indicated by a prominent concern over Poor complexion or skin trouble. The analysis of this data has brought into focus some of the physical, mental and emotional health needs of adolescents. Based upon these findings, recommendations wore suggested for improving the curriculum in health by: (1) the school administrator and staff using the data as a beginning assessment to discover physical and emotional needs which might serve as a baseline for a study in health) (2) studying the findings as a part of an in-service education program for teachers might lead to a fuller understanding of students\u27 needs, problems and interests) (3) giving study to the medical record format to discourage sketchy reporting by private physicians; (4) acquainting parents with the findings on records that indicate needs for improving mental health) and (5) implementing a gradual improvement in health teaching in which the item of highest frequency might receive first attention. Realizing that the school is in a strategic position to meet the health needs of the students, it is hoped that the findings of this survey will pave the way for further studies

Effect of Pneumatic Tubing on Regular Insulin Concentration

Class of 2016 Abstract and ReportObjectives: To describe the effect of time spent in pneumatic tube system on the concentrations of bags of regular insulin. Methods: Twelve intravenous bags of regular insulin in normal saline with a concentration of one unit per milliliter were prepared, with six bags acting as the control group and six bags as the experimental group. Bags in the experimental group were transported to stations labeled X, Y, and Z which were at varying distances from the pharmacy. Bags in the control group were walked the same tube stations. Three samples from each bag were analyzed using the ValiMed™ medication validation system before and after transport and the standard deviations (SDs) from the mean were recorded. Results: At baseline there were no statistically significant differences in the standard deviations (SDs) between the control and experimental group (p = 0.1008). SDs after transport compared to baseline SDs produced statistically significant differences (p < 0.005) except for the control group transported to tube station Z (p = 0.0867). Conclusions: The SDs after either transport produced a statistically significant difference when compared to baseline except for one group of insulin bags. This indicates that concentration may not be affected by method of delivery, since statistically significant difference occurred regardless of transport method. It appears to be safe to transport insulin IV infusion bags by pneumatic tube system.This item is part of the Pharmacy Student Research Projects collection, made available by the College of Pharmacy and the University Libraries at the University of Arizona. For more information about items in this collection, please contact Jennifer Martin, Librarian and Clinical Instructor, Pharmacy Practice and Science, [email protected]

Assessing the Effectiveness of the University of Arizona College of Pharmacy's Student-Run Health Screening Events at Reaching Underserved Tucson Populations

Class of 2014 AbstractSpecific Aims: To assess the population that attends The College of Pharmacy’s health fairs and determine which fairs best cater to Tucson communities that benefit the most from free health services. Methods: Data collection occurred throughout two semesters, fall of 2012 and spring of 2013. Information was obtained from screening tools administered at health fairs. Access to insurance, whether patients had previously seen a physician for screened condition, and the amount of referrals were gathered for data analysis. Main Results: Significant differences were found between the health fairs in categories, i.e. patients with/without insurance, had/had not previously seen their physician and were/were not referred. Conclusion: Study results can be used to help in the process of scheduling health fairs for subsequent academic years. A criterion should be generated to make decisions for reconsideration and to better allocate the College’s resources.This item is part of the Pharmacy Student Research Projects collection, made available by the College of Pharmacy and the University Libraries at the University of Arizona. For more information about items in this collection, please contact Jennifer Martin, Librarian and Clinical Instructor, Pharmacy Practice and Science, [email protected]

Evidence for the negative regulation of phytase gene expression in Streptomyces lividans and Streptomyces coelicolor.

International audienceSco7697, a gene encoding a phytase, enzyme able to degrade phytate (myo-inositol 1,2,3,4,5,6-hexakis phosphate), the most abundant phosphorus storing compound in plants is present in the genome of S. coelicolor, a soil born bacteria with a saprophytic lifestyle. The expression of this gene was previously shown to be induced in conditions of Pi limitation by the response regulator PhoP binding to an operator sequence, the PHO box, located upstream of the -35 promoter sequence. A close examination of the promoter region of sco7697 revealed the presence of another putative operator site, a Direct Repeat (DR), located downstream of the -10 promoter sequence. In order to determine whether this DR played a role in regulation of sco7697 expression, different variants of the phytase gene promoter region were transcriptionally fused to the ß-glucuronidase reporter gene (GUS). As expected, deletion of the PHO box led to abolition of sco7697 induction in conditions of Pi limitation. Interestingly, alteration of the DR correlated with a dramatic increase of GUS expression but only when PhoP was present. These results demonstrated that this DR is the site of strong negative regulation by an unknown repressor. The latter would impede the necessary activation of phytase expression by PhoP