All roads lead to auxin: Post-translational regulation of auxin transport by multiple hormonal pathways

Auxin is a key hormonal regulator, that governs plant growth and development in concert with other hormonal pathways. The unique feature of auxin is its polar, cell-to-cell transport that leads to the formation of local auxin maxima and gradients, which coordinate initiation and patterning of plant organs. The molecular machinery mediating polar auxin transport is one of the important points of interaction with other hormones. Multiple hormonal pathways converge at the regulation of auxin transport and form a regulatory network that integrates various developmental and environmental inputs to steer plant development. In this review, we discuss recent advances in understanding the mechanisms that underlie regulation of polar auxin transport by multiple hormonal pathways. Specifically, we focus on the post-translational mechanisms that contribute to fine-tuning of the abundance and polarity of auxin transporters at the plasma membrane and thereby enable rapid modification of the auxin flow to coordinate plant growth and development

A review of deep learning applications for the next generation of cognitive networks

Intelligence capabilities will be the cornerstone in the development of next-generation cognitive networks. These capabilities allow them to observe network conditions, learn from them, and then, using prior knowledge gained, respond to its operating environment to optimize network performance. This study aims to offer an overview of the current state of the art related to the use of deep learning in applications for intelligent cognitive networks that can serve as a reference for future initiatives in this field. For this, a systematic literature review was carried out in three databases, and eligible articles were selected that focused on using deep learning to solve challenges presented by current cognitive networks. As a result, 14 articles were analyzed. The results showed that applying algorithms based on deep learning to optimize cognitive data networks has been approached from different perspectives in recent years and in an experimental way to test its technological feasibility. In addition, its implications for solving fundamental challenges in current wireless networks are discussed

Modulation of plant root growth by nitrogen source-defined regulation of polar auxin transport

Availability of the essential macronutrient nitrogen in soil plays a critical role in plant growth, development, and impacts agricultural productivity. Plants have evolved different strategies for sensing and responding to heterogeneous nitrogen distribution. Modulation of root system architecture, including primary root growth and branching, is among the most essential plant adaptions to ensure adequate nitrogen acquisition. However, the immediate molecular pathways coordinating the adjustment of root growth in response to distinct nitrogen sources, such as nitrate or ammonium, are poorly understood. Here, we show that growth as manifested by cell division and elongation is synchronized by coordinated auxin flux between two adjacent outer tissue layers of the root. This coordination is achieved by nitrate‐dependent dephosphorylation of the PIN2 auxin efflux carrier at a previously uncharacterized phosphorylation site, leading to subsequent PIN2 lateralization and thereby regulating auxin flow between adjacent tissues. A dynamic computer model based on our experimental data successfully recapitulates experimental observations. Our study provides mechanistic insights broadening our understanding of root growth mechanisms in dynamic environments

A precision medicine test predicts clinical response after idarubicin and cytarabine induction therapy in AML patients

Complete remission (CR) after induction therapy is the first treatment goal in acute myeloid leukemia (AML) patients and has prognostic impact. Our purpose is to determine the correlation between the observed CR/CRi rate after idarubicin (IDA) and cytarabine (CYT) 3 + 7 induction and the leukemic chemosensitivity measured by an ex vivo test of drug activity. Bone marrow samples from adult patients with newly diagnosed AML were included in this study. Whole bone marrow samples were incubated for 48 h in well plates containing IDA, CYT, or their combination. Pharmacological response parameters were estimated using population pharmacodynamic models. Patients attaining a CR/CRi with up to two induction cycles of 3 + 7 were classified as responders and the remaining as resistant. A total of 123 patients fulfilled the inclusion criteria and were evaluable for correlation analyses. The strongest clinical predictors were the area under the curve of the concentration response curves of CYT and IDA. The overall accuracy achieved using MaxSpSe criteria to define positivity was 81%, predicting better responder (93%) than non-responder patients (60%). The ex vivo test provides better yet similar information than cytogenetics, but can be provided before treatment representing a valuable in-time addition. After validation in an external cohort, this novel ex vivo test could be useful to select AML patients for 3 + 7 regimen vs. alternative schedules

A922 Sequential measurement of 1 hour creatinine clearance (1-CRCL) in critically ill patients at risk of acute kidney injury (AKI)

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Time to Switch to Second-line Antiretroviral Therapy in Children With Human Immunodeficiency Virus in Europe and Thailand.

Background: Data on durability of first-line antiretroviral therapy (ART) in children with human immunodeficiency virus (HIV) are limited. We assessed time to switch to second-line therapy in 16 European countries and Thailand. Methods: Children aged <18 years initiating combination ART (≥2 nucleoside reverse transcriptase inhibitors [NRTIs] plus nonnucleoside reverse transcriptase inhibitor [NNRTI] or boosted protease inhibitor [PI]) were included. Switch to second-line was defined as (i) change across drug class (PI to NNRTI or vice versa) or within PI class plus change of ≥1 NRTI; (ii) change from single to dual PI; or (iii) addition of a new drug class. Cumulative incidence of switch was calculated with death and loss to follow-up as competing risks. Results: Of 3668 children included, median age at ART initiation was 6.1 (interquartile range (IQR), 1.7-10.5) years. Initial regimens were 32% PI based, 34% nevirapine (NVP) based, and 33% efavirenz based. Median duration of follow-up was 5.4 (IQR, 2.9-8.3) years. Cumulative incidence of switch at 5 years was 21% (95% confidence interval, 20%-23%), with significant regional variations. Median time to switch was 30 (IQR, 16-58) months; two-thirds of switches were related to treatment failure. In multivariable analysis, older age, severe immunosuppression and higher viral load (VL) at ART start, and NVP-based initial regimens were associated with increased risk of switch. Conclusions: One in 5 children switched to a second-line regimen by 5 years of ART, with two-thirds failure related. Advanced HIV, older age, and NVP-based regimens were associated with increased risk of switch

Wounding-induced changes in cellular pressure and localized auxin signalling spatially coordinate restorative divisions in roots

Wound healing in plant tissues, consisting of rigid cell wall-encapsulated cells, represents a considerable challenge and occurs through largely unknown mechanisms distinct from those in animals. Owing to their inability to migrate, plant cells rely on targeted cell division and expansion to regenerate wounds. Strict coordination of these wound-induced responses is essential to ensure efficient, spatially restricted wound healing. Single-cell tracking by live imaging allowed us to gain mechanistic insight into the wound perception and coordination of wound responses after laser-based wounding in Arabidopsis root. We revealed a crucial contribution of the collapse of damaged cells in wound perception and detected an auxin increase specific to cells immediately adjacent to the wound. This localized auxin increase balances wound-induced cell expansion and restorative division rates in a dose-dependent manner, leading to tumorous overproliferation when the canonical TIR1 auxin signaling is disrupted. Auxin and wound-induced turgor pressure changes together also spatially define the activation of key components of regeneration, such as the transcription regulator ERF115. Our observations suggest that the wound signaling involves the sensing of collapse of damaged cells and a local auxin signaling activation to coordinate the downstream transcriptional responses in the immediate wound vicinity

Prioritization-Driven Congestion Control in Networks for the Internet of Medical Things: A Cross-Layer Proposal

Real-life implementation of the Internet of Things (IoT) in healthcare requires sufficient quality of service (QoS) to transmit the collected data successfully. However, unsolved challenges in prioritization and congestion issues limit the functionality of IoT networks by increasing the likelihood of packet loss, latency, and high-power consumption in healthcare systems. This study proposes a priority-based cross-layer congestion control protocol called QCCP, which is managed by communication devices’ transport and medium access control (MAC) layers. Unlike existing methods, the novelty of QCCP is how it estimates and resolves wireless channel congestion because it does not generate control packets, operates in a distributed manner, and only has a one-bit overhead. Furthermore, at the same time, QCCP offers packet scheduling considering each packet’s network load and QoS. The results of the experiments demonstrated that with a 95% confidence level, QCCP achieves sufficient performance to support the QoS requirements for the transmission of health signals. Finally, the comparison study shows that QCCP outperforms other TCP protocols, with 64.31% higher throughput, 18.66% less packet loss, and 47.87% less latency

A biostratigraphical review of the Middle Ordovician shales from NW Spain (Cantabrian and Westasturian-Leonese zones, and northernmost part of the Central Iberian Zone)

La revisión completa de más de un centenar de localidades fosilíferas del Ordovícico Medio situadas en el noroeste del Macizo Hespérico, muestra que el depósito de las pizarras y limolitas oscuras (Formación Luarca y equivalentes), que siguen a las cuarcitas del Arenig, no fue tan uniforme como se consideraba hasta ahora. Las pizarras se sedimentaron esencialmente durante el Oretaniense en la Zona Asturoccidental-Leonesa y en la parte septentrional de la Zona Centroibérica (Dominio del Ollo de Sapo), donde el techo de la unidad se sitúa muy próximo al límite Oretaniense / Dobrotiviense, sin existir ningún yacimiento paleontológico de probada edad dobrotiviense (= "Llandeilo inferior" en sentido clásico). En la Zona Cantábrica, la sedimentación arcillosa se inicia en el Oretaniense superior tardío y prosigue durante el Dobrotiviense. Para el conjunto del área estudiada se caracterizan, o proponen, diversas lagunas estratigráficas de alcance local o regional, y se discuten las circunstancias paleogeográficas generadas por las hipótesis más recientes, que son contrastadas a la luz de los nuevos datos paleoecológicos y paleobiogeográficos. Éstos indican que la sedimentación tuvo lugar en áreas externas y abiertas de la plataforma, relativamente más profundas que en la región surcentroibérica, con áreas de surco donde ingresan elementos mesopelágicos. Desde el punto de vista paleontológico, se reconocieron un total de 97 formas distintas (67 especies presentes en el Oretaniense y 45 en el Dobrotiviense), entre las que destaca la primera aparición conocida de determinadas especies de trilobites y ostrácodos, el registro más moderno de otros, y algunos taxones en común con Avalonia y Baltica, desconocidos hasta el presente en otras áreas del suroeste de EuropaA complete review of more than one hundred Middle Ordovician fossil localities, distributed within the north western part of the Hesperian Massif, shows that the sedimentation of the dark shales (Luarca Fo rmation and equivalents) that overlie Arenig quartzites with Cruziana (the 'Armorican Quartzite' facies) was not as uniform as it has been supposed for the whole NW Spain. These shales were mainly deposited during the Oretanian in the West Asturian-Leonese Zone and in nort h e rn Central-Iberian Zone (Domain of the Ollo de Sapo Antiform). In these zones, the top of the unit is close to the Oretanian/Dobrotivian boundary, without any fossils with proved Dobrotivian age. In the Cantabrian Zone, clay sedimentation started in the latest Oretanian and continued during the Dobrotivian. Several local or regional stratigraphic gaps are proposed and characterized for the whole study area. In addition, the most recent paleog e ographical recons t ructions proposed are discussed, in accordance with new paleoecological and paleobiog e ographical data. These data indicate that sedimentation took place in open shelf areas, relative ly deeper than in the southern Central Iberian shelf, and with trough areas where some mesopelagic elements are recorded. We identified a total of 97 different fossil taxa (67 from Oretanian rocks and 45 from Dobrotiv i a n rocks), remarkable among which are the first known appearance of certain trilobites and ostracodes, the latest record of other taxa, and also the presence of some taxa in common with Avalonia and Baltica, that were prev i o u s ly unknown from any area of SW EuropeMinisterio de Educación y CulturaComunidad de MadridDepto. de Geodinámica, Estratigrafía y PaleontologíaFac. de Ciencias GeológicasTRUEpu

An IoT System for Remote Health Monitoring in Elderly Adults through a Wearable Device and Mobile Application

With the increase in global life expectancy and the advance of technology, the creation of age-friendly environments is a priority in the design of new products for elderly people healthcare. This paper presents a proposal for a real-time health monitoring system of older adults living in geriatric residences. This system was developed to help caregivers to have a better control in monitoring the health of their patients and have closer communication with their patients&#8217; family members. To validate the feasibility and effectiveness of this proposal, a prototype was built, using a biometric bracelet connected to a mobile application, which allows real-time visualization of all the information generated by the sensors (heart rate, body temperature, and blood oxygenation) in the bracelet. Using these data, caregivers can make decisions about the health status of their patients. The evaluation found that the users perceived the system to be easy to learn and use, providing initial evidence that our proposal could improve the quality of the adult&#8217;s healthcare