Population and conservation genetic structure of the Cascades frog, Rana cascadae throughout the species' range

A major goal of conservation biology is to elucidate the population genetic structure in threatened species and assess the relative importance of the evolutionary forces that shape that population genetic structure. I conducted three studies in the declining amphibian Rana cascadae to assess levels of population genetic differentiation and the relative importance of gene flow versus random genetic drift throughout the species' range. In the first study, 1 examined phylogeographic structure on a species-wide geographic scale with both mitochondrial and nuclear molecular markers. I found three mitochondrial groups within R. cascadae that are as divergent at the mitochondrial DNA as sister species. However, I only found two nuclear groups within R. cascadae, suggesting there are two Distinct Population Segments and three Management Units within the species' range. In the second study, I compared sequence data from mtDNA and nuclear DNA of the three R. cascadae mtl)NA groups to several closely related Pacific Northwestern ranid species. I found the surprising result that the mtDNA of R. aurora aurora is more closely related to the mtDNA of' R. cascadae than to the mtDNA of its own subspecies R. aurora drayloni. The nuclear data support the sub-specific relationship between R. aurora aurora and R. aurora draytoni. This result is most likely due to incomplete lineage sorting of ancestral mtDNA alleles. Finally, in the third study, I examined the relative importance of gene flow versus random genetic drift on a fine geographic scale using microsatellite loci. Additionally, I estimated the long-term effective population sizes and genetic neighborhood size for 11 R. cascadae populations. Rana cascadae shows extreme isolation by distance with very little gene flow occurring past a distance of 10 km. Long-term effective population sizes were unrealistically large for current effective population sizes, but the estimates oF genetic neighborhood size are consistent with those expected based on current census population size and genetic neighborhood size in other amphibians. My research suggests Rana cascadae should be managed as three separate groups corresponding to the Olympic Peninsula, the Cascades of Washington and Oregon, and Northern California. Additionally, R. cascadae exhibits extreme isolation by distance with reduced gene flow at distances greater than 10 km, suggesting metapopulation structure is weak, and populations that go extinct are unlikely to be re-colonized quickly despite the presence of nearby R. cascadae populations

Extreme isolation by distance in a montane frog Rana cascadae. Conservation Genetics

Abstract Given the recent interest in declining amphibian populations, it is surprising that there are so few data on genetic drift and gene flow in anuran species. We used seven microsatellite loci to investigate genetic structure and diversity at both large and small geographic scales, and to estimate gene flow in the Cascades frog, Rana cascadae. We sampled 18 sites in a hierarchical design (inter-population distances ranging from 1-670 km) to test for isolation by distance and to determine the geographic scale over which substantial gene flow occurs. Eleven of these sites were sampled as three fine-scale clusters of three, three, and five sites separated by pairwise distances of 1-23 km to estimate number of migrants exchanged per generation via F ST and by a coalescent approach. We found R. cascadae exhibits a strong pattern of isolation by distance over the entire species range, and that there is a sharp drop in migrants exchanged between sites separated by greater than 10 km. These data, in conjunction with results of other recent studies, suggest that montane habitats promote unusually strong genetic isolation among frog populations. We discuss our results in light of future management and conservation of R. cascadae

Structure, Biodiversity, and Historical Biogeography of Nematode Faunas in Holarctic Ruminants: Morphological and Molecular Diagnoses for \u3ci\u3eTeladorsagia boreoarticus\u3c/i\u3e n. sp. (Nemadota: Ostertagiinae), Dimorphic Cryptic Species in Muskoxen (\u3ci\u3eOvibos moschatus\u3c/i\u3e)

Discovery of the ostertagiine nematode Teladorsagia boreoarcticus n. sp. in muskoxen, Ovibos moschatus, from the central Canadian Arctic highlights the paucity of knowledge about the genealogical and numerical diversity of nematode faunas characteristic of artiodactyls at high latitudes across the Holarctic. Teladorsagia boreoarcticus is a dimorphic cryptic species distinguished from Teladorsagia circumcincta/Teladorsagia trifurcata in domestic sheep by a 13% divergence in the ND4 region of mitochondrial DNA, constant differences in the synlophe, and significantly longer esophageal valve, spicules, gubernaculum, and bursa. Teladorsagia boreoarcticus represents an archaic component of the North American fauna and may have a Holarctic distribution in muskoxen and caribou. Recognition of T. boreoarcticus in muskoxen, in part, corroborates hypotheses for the existence of a cryptic species complex of Teladorsagia spp. among Caprinae and Cervidae at high latitudes and indicates the importance of climatological determinants during the late Tertiary and Pleistocene on diversification of the fauna. Also reinforced is the concept of the North American fauna as a mosaic of endemic and introduced species. Discovery of a previously unrecognized species of Teladorsagia has additional implications and clearly indicates that (1) our knowledge is incomplete relative to potentially pathogenic nematodes that could be exchanged among domestic and wild caprines; (2) we do not have sufficient knowledge of the fauna to understand the ecological control mechanisms (limitations) on dissemination and host range; and (3) an understanding of historical and geographical influences on the genealogical diversity and distribution of nematode faunas in domestic and wild ruminants is requisite to define the interface between agricultural and natural ecosystems across the Holarctic

Direct and Indirect Analysis of the Fitness of Chrysochus (Coleoptera: Chrysomelidae) Hybrids

The chrysomelid beetles Chrysochus auratus and C. cobaltinus form a narrow hybrid zone in western North America. We used a combination of direct and indirect analyses to examine the fitness of Chrysochus hybrids. For the direct analyses, we compared the mating frequency, longevity, fecundity and fertility of hybrid females and parentals. For the indirect approach, we tested predictions of multilocus genotype frequencies at a focal site in the hybrid zone, based on the frequencies of mating combinations during the previous generation. Hybrid females produced fewer eggs than did parentals and the eggs they produced in the lab failed to hatch, in contrast to those of parental females. In addition, contrary to predictions that 15.8% of the individuals at the focal site would have multilocus genotypes other than those expected of parentals or F1 individuals, we found no such genotypes at this site. This hybrid zone appears to be an example of a classic tension zone, with endogenous selection against hybrid individuals. We discuss the implications of low hybrid fitness for the evolution of premating barriers in this system, and argue that the integration of direct and indirect approaches is a powerful means of assessing the relative fitness of hybrids, particularly for species in which mate choices are easy to observe in the field

Comparative Analyses Of Effective Population Size Within And Among Species: Ranid Frogs As A Case Study

It has recently become practicable to estimate the effective sizes (N e) of multiple populations within species. Such efforts are valuable for estimating N e in evolutionary modeling and conservation planning. We used microsatellite loci to estimate N e of 90 populations of four ranid frog species (20-26 populations per species, mean n per population = 29). Our objectives were to determine typical values of N e for populations of each species, compare N e estimates among the species, and test for correlations between several geographic variables and N e within species. We used single-sample linkage disequilibrium (LD), approximate Bayesian computation (ABC), and sibship assignment (SA) methods to estimate contemporary N e for each population. Three of the species-Rana pretiosa, R. luteiventris, and R. cascadae- have consistently small effective population sizes (\u3c50). N e in Lithobates pipiens spans a wider range, with some values in the hundreds or thousands. There is a strong east-to-west trend of decreasing N e in L. pipiens. The smaller effective sizes of western populations of this species may be related to habitat fragmentation and population bottlenecking. © 2011 The Author(s). Evolution © 2011 The Society for the Study of Evolution

Rana cascadae microsatellite data

GENEPOP input file

Rana luteiventris microsatellite data

GENEPOP input file

Rana pretiosa microsatellite data

GENEPOP input file

Lithobates (Rana) pipiens microsatellite data

Lithobates (Rana) pipiens microsatellite dat

The Effects of Heterospecific Mating Frequency on the Strength of Cryptic Reproductive Barriers

Heterospecific mating frequency is critical to hybrid zone dynamics and can directly impact the strength of reproductive barriers and patterns of introgression. The effectiveness of post‐mating prezygotic (PMPZ) reproductive barriers, which include reduced fecundity via heterospecific matings and conspecific sperm precedence, may depend on the number, identity and order of mates. Studies of PMPZ barriers suggest that they may be important in many systems, but whether these barriers are effective at realistic heterospecific mating frequencies has not been tested. Here, we evaluate the strength of cryptic reproductive isolation in two leaf beetles (Chrysochus auratus and C. cobaltinus) in the context of a range of heterospecific mating frequencies observed in natural populations. We found both species benefited from multiple matings, but the benefits were greater in C. cobaltinus and extended to heterospecific matings. We found that PMPZ barriers greatly limited hybrid production by C. auratus females with moderate heterospecific mating frequencies, but that their effectiveness diminished at higher heterospecific mating frequencies. In contrast, there was no evidence for PMPZ barriers in C. cobaltinus females at any heterospecific mating frequency. We show that integrating realistic estimates of cryptic isolation with information on relative abundance and heterospecific mating frequency in the field substantially improves our understanding of the strong directional bias in F1 production previously documented in the Chrysochus hybrid zone. Our results demonstrate that heterospecific mating frequency is critical to understanding the impact of cryptic post‐copulatory barriers on hybrid zone structure and dynamics, and that future studies of such barriers should incorporate field‐relevant heterospecific mating frequencies