Prevalence of JAK2 V617F Mutation in Iranian Patients with Myeloproliferative Neoplasms

Background and Aim: Multiple lines of evidence have been suggested that JAK2 is likely the main candidate gene responsible for the pathogenesis of myeloproliferative neoplasms. The V617F mutation in the pseudokinase domain of JAK2 protein has been detected in a majority of patients. We aimed to evaluate the frequency of this somatic missense substitution among Iranian patients with myeloproliferative neoplasms. Methods Peripheral blood samples were collected from patients with myeloproliferative neoplasms across different regions of Iran. The JAK2 V617F mutation was identified by allele-specific PCR. To confirm the PCR results, randomly selected positive and negative samples were sequenced. Results: Among 72 identified patients, 45 (62.5%) were found to harbor JAK2 V617F. The frequencies of the mutation ranged 100% for primary myelofibrosis, 75% for chronic myelogenous leukemia, 67% for polycythemia vera, 62.5% for myelodysplastic/myeloproliferative neoplasms, and 52% for essential thrombocythemia. Our findings revealed that the mutation was more common among men in comparison with women and the correlation between the mutation and gender was statistically significant (p-value<0.01). Additionally, the presence of JAK2 V617F was associated with older ages (p-value =0.009). Conclusion: The JAK2 V617F mutation was detected in 62.5% of patients with myeloproliferative neoplasms. We have shown that this single acquired point mutation was presented in at least half of the patients. Hence, it seems that the identification of JAK2 V617F mutation in myeloproliferative neoplasms can be very effective in disease diagnosing and management. *Corresponding Author: Mohammad Hamid; Email: [email protected] Please cite this article as: Hamid M, Shahbaz Z. Prevalence of JAK2 V617F Mutation in Iranian Patients with Myeloproliferative Neoplasms. Arch Med Lab Sci. 2020;6:1-7 (e5). https://doi.org/10.22037/amls.v6.3275

High order algorithms for numerical solution of fractional differential equations

This document is the Accepted Manuscript version of a published work that appeared in final form in [Advances in Difference Equations]. To access the final edited and published work see http://dx.doi.org/10.1186/s13662-021-03273-4.In this paper, two novel high order numerical algorithms are proposed for solving fractional differential equations where the fractional derivative is considered in the Caputo sense. The total domain is discretized into a set of small subdomains and then the unknown functions are approximated using the piecewise Lagrange interpolation polynomial of degree three and degree four. The detailed error analysis is presented, and it is analytically proven that the proposed algorithms are of orders 4 and 5. The stability of the algorithms is rigorously established and the stability region is also achieved. Numerical examples are provided to check the theoretical results and illustrate the efficiency and applicability of the novel algorithms

INVESTIGATION OF IRREGULAR EFFECTS IN HEIGHT ON CONCRETE STRUCTURES BEHAVIOR COEFFICIENT

Abstract. The behavior of structures at the time of the occurrence of large earthquakes enters thenonresponsive range, and their design requires a nonresponsive analysis, but due to the costly nature of this method and its effect to enhance the effect of nonresponsive behavior and energy dissipation due to hysteresis behavior, The damping and the effect of the excessive strength of the structure transform this reactionary force by the coefficient of reduction of resistance or the coefficient of behavior into the design force. Determining the magnitudes of these coefficients in earthquake regulations is mainly based on performance observations of building systems in past earthquakes and based on engineering judgments. One of the cases that has a significant effect on the behavior of structures is irregularity in height, which according to researchers many expressed their concern about the lack of reasonable behavioral coefficients based on research studies and computational backing and emphasized on thecorrection of these coefficients based on scientific studies. In this study, using finite element software, irregular effects in the defense is studied on the coefficient of behavior of concrete structures studied is. In the present study, 30 flexural concrete frames have been studied regularly and irregularly in structures with 3, 6, 9, 12, 15 floors in elevations with different openings of 4, 6 and 8 meters. The structures were selected for elastic analysis in order to design, model, and load. After this stage, the structures were modeled for non-elastic analysis (nonlinear static analysis with increasing lateral loading) and then the coefficient of structure behavior was calculated. According to the results, in most cases, the coefficient of reduction due to ductility (R μ ) in regular concrete structures at altitude ismore than that of irregular structures, in other words, in structures with the same number of floors and openings, concrete structures regularly, they have better fitting height than irregular structures at elevation. In particular, it can be said that the coefficient of reduction due to irregular structure (Rμ) in irregular structures at elevation is 91% of its value in the structure regularly in height, in this study, with increasing the number of floors from 3 to 6, the resistance coefficient is decreasing further, but from 6 floors to then, in structures 9, 12 and 15, there is no significant change in the resistivity coefficient. Also, the regular or irregularity of the structure did not make any difference in the change in the value of this coefficient. The overall behavior of the structure (R) also affects theregular concrete structures at altitude there is a greater amount than the irregular structures at elevation. The regular structures with the number of floors and the size of the span have a higher coefficient of behavior than the irregular structures at elevation. The results showed that the coefficient of behavior obtained from nonlinear dynamic analysis the linear average is about 15% higher than the values obtained by nonlinear static analysis with making side are increasing and there is little difference between the two methods.Keywords: Structural behavior coefficient -irregularity at height-concrete structures

Today nursing need for emotional intelligence: Integrative review of literature‏

Introduction: Emotional intelligence capabilities play a key role in determining an individual’s success in health care organizations. These skills enable individuals to think properly in difficult conditions and prevent wasting emotions such as anger, anxiety and fear and thus calm themselves and open new gateways for insight and self reflection and creative ideas. Therefore, considering the undeniable role of emotional intelligence in the success of nurses is very important. Aim:The purpose of this study is that emotional intelligence and nursing response What is the use? Method: This study is a systematic review, with using key words education, emotional intelligence and nursing, among about 100 research and review papers in Persian and English in the field of nursing and health, has been used electronic databases CINAHL ،science direct ،Ovid ،Scopus ، SID ، Magiran ،Pubmed , Google scholar and 30 article assessed finally. This study has been in 15- year period from 1997 to 2012. Finally, 30 articles in Persian and English, include keywords, which were peer review published in ISI journals, were selected and assessed. Results: The results of this study show that emotional intelligence training is considered as a revolution and vital need in nursing, because it creates a sustainable guideline for developing creativity in the nurse-client relationship and widens personal and professional limits and leads to the wise control of relationships between the nurse and the patient, and causes the nurse to have a human viewpoint about the patient, Hence, emotional intelligence training should be incorporated in nursing programs. Conclusion: : Analysis of results of studies indicate that emotional intelligence to gain skills, with a tremendous impact in improving services to patients, families and entire communities will not only prevent loss of financial resources But fundamental changes in perspective and attitude of nurses towards patients, and their jobs as a professional skill, creates, can also promote and enhance the profession. Therefore, to gain emotional intelligence skills for nurses, is need for nowadays nursing and requirements of today’s professional activities related to nursing personnel and other employees in the health system. Nursing managers obtain EQ skills, with a tremendous impact in improving the services provided to patients, families and the entire community will not only prevent the waste of financial and human resources professionals is improving

Gingival Melanin Depigmentation Using a Diode 808-nm Laser: A Case Series

Background: Gingival hyperpigmentation is caused by an increase in melanin production or the number of melanocytes in the epithelium, which can impair smile esthetics. The current study aimed to report the experience of using diode laser for gingival depigmentation of three different patients. Case Presentation: Three adult patients are presented with hyperpigmentation in labial gingivae of both jaws. The depigmentation was done using a diode laser at a wavelength of 808 nm, spot diameter of 300 μm, power of 1.8 W, and in continuous mode. All three patients showed proper resolution of hyperpigmented areas in short- and long-term follow-up sessions. However, the recurrence rate was more rapid in heavy smoker patient. Conclusion: As an alternative to other treatment modalities, the 808 nm diode laser can be utilized safely and efficiently in gingival depigmentation, resulting in an acceptable cosmetic outcome

Site selection of rural waste landfill using the AHP model and GIS software (Case study: the central part of Ejroud city)

Background and Aims: Site selection and management of a landfill site is one of the main pillars of sustainable development. Selection of an appropriate site for the burial of rural wastes is of utmost importance in rural areas.  At present there is no coherent management in the field of rural wastes. Selecting the right place for landfilling requires considering several complex factors and thus the need for use of spatial information technologies and their incorporation to other managing and planning issues are raised.Materials and Methods: In this study, nine important affecting parameters in locating the waste burial site including the slope, aspect, elevation, distance to the rural points, distance to the channels, distance to the roads, distance to the faults, erosion, and land use were considered to assess the appropriate site for burial of the rural wastes of the central part of Ejroud city. The identified parameters were compared by incorporating the Analytic Hierarchy Analysis (AHP) method in a pairwise manner and the corresponding weight of each factor, which indicates the effect of that factor, was calculated utilizing the Expert Choice software. All stages of this research were conducted ethically.Results: Through the incorporation of weight layers obtained in the ArcGIS  environment, the final locating map for burial of the rural wastes in the study area was prepared and categorized into 5 classes (completely inappropriate, inappropriate, intermediate, appropriate and completely appropriate).Conclusion: The results showed that the appropriate and completely appropriate zones with 251.1 and 158.1 square kilometers had respectively the highest prefer ability for burial of the wastes in this categorization. Totally, about 41.8% of the total area of the town, was prioritized for the waste burial plans.

Mechanical damage to pinto beans as affected by moisture content and impact energy

 Mechanical damage to seeds due to harvest, handling and other process is an important factor that affects the quality of seeds.  Seed damage results in lower grain value, storability problem, and reducing of seed germination and seedling vigor and subsequent yield of crops.  Tests were conducted to determine the extent of percentage of physical damage (PPD) and percentage of loss in germination (PLG) (physiological damage) of pinto beans due to impact.  The effects of beans moisture content (9.25%, 12.51%, 15.02% and 17.50%, wet basis) and impact energy (0.09 J, 0.19 J and 0.29 J) were determined.  The tests were conducted under laboratory conditions, using an impact test apparatus.  Results showed that effects of moisture content and impact energy on seed damages were significant.  PPD of beans was higher than PLG.  It found that the total damage of beans increased from 54.45% (48.14% PPD and 6.31% PLG) to 73.20% (63.40% PPD and 9.80% PLG) as the impact energy increased from 0.9 to 0.29 J, for all the moisture contents used.  With increasing the moisture content from 9.25% to 17.50%, the mean values of the percentage of loss in germination of beans increased from 0.53 to 15.30%.  However, by increasing in the moisture from 9.25% to 17.50%, the mean values of percentage of physical damage to beans decreased from 92.67% to 21.53%. Keywords: physical damage, reduce in germination, moisture content, impact energy, pinto bean 

KINEMATIC AND KINETIC COMPARISONS BElWEEN SPOT, CROSSOVER AND UPWARD JUMP THROWINGS IN HANDBALL

Throwing is one of the most important skills in handball in which two basic factors are of importance with regard to the efficiency of shots Le. accuracy and velocity. A 2Dimensional analysis of different throws Le. on the spot, with a cross-over step, and with upward jump has been undertaken. Eighteen high-performance handball players took part in this study. The average values of basic parameters of physical characteristics of subjects were: 80.2 ± 6.1 Kg (body mass), 184.8 ± 4 cm (body height), and 19.79 ± 0.63 years of age. The main aim of present study was to establish a valuable kinematic and kinetic comparison between different types of throws in handball. Statistically significant differences were found between maximal ban velocity during throws with cross-over step and ball velocity during other analyzed throws. In addition to the high velocity in this throw, the energy and power consumptions were also higher in magnitude comparing with other throws. The results obtained by this study agreed well with the results reported by other researchers

Care of newborns born to mothers with COVID-19 infection; a review of existing evidence

Background:The novel Coronavirus disease 2019 (COVID-19) pandemic is already wreaking havoc on families and communities\u27 welfare. It is critical to discuss newborn care of infected mothers with COVID-19 based on the latest international guidelines and national guidelines of countries with the highest incidence of COVID-19 cases. Objective:We discuss how to care for a newborn of a suspected or infected mother with COVID-19 using existing evidence. Method:As of 16 April 2020, we reviewed articles and guidelines related to COVID-19 in the reproductive health field, mother, and newborn health. Our review yielded in 10 categories (i) the risk of diagnostic procedures in suspected mothers on fetus/infant health, (ii) the risk of intrauterine or postpartum transmission to the fetus/infant, (iii) appropriate method and delivery time in women with confirmed COVID-19, (iv) umbilical cord clamping and skin to skin contact, (v) clinical manifestations of infected infants, (vi) confirmation of infection in a suspected neonate/infant, (vii) instructions for infant\u27s care and how to feed her/him, (viii) bathing the baby, (ix) the criteria of discharging baby from the hospital, (x) the impact of isolation on the maternal mental health. Results:Our findings showed that the possibility of intrauterine or perinatal transmission of COVID-19 is still questionable and ambiguous. However, what has been agreed upon in the existing texts and guidelines is that the close contact of mother and infant after birth can transmit the virus to the baby through droplets or micro-droplets. Conclusions:Based on our findings, it is recommended to separate the baby from the mother with confirmed (or suspected) COVID-19 infection for at least 2 weeks. Since the motivation and stable situation of mothers allow breastfeeding during the isolation, infected mothers should be taught about breast expression skills, common breast problems, the symptoms of their baby\u27s infection, and the principles of personal hygiene to protect the infant against COVID-19 infection